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Q9C5C0

- MPK18_ARATH

UniProt

Q9C5C0 - MPK18_ARATH

Protein

Mitogen-activated protein kinase 18

Gene

MPK18

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 4 (31 May 2011)
      Previous versions | rss
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    Functioni

    Mitogen-activated protein kinase (MAPK) that is specifically regulated by PHS1 and mediates signaling that regulates cortical microtubule functions, maybe through regulation of microtubule dynamic instability.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation Probable. Inactivated by phosphatase PHS1.1 PublicationCurated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541ATPPROSITE-ProRule annotation
    Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: TAIR
    3. protein binding Source: IntAct

    GO - Biological processi

    1. cortical microtubule organization Source: TAIR
    2. MAPK cascade Source: GOC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G53510-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 18 (EC:2.7.11.24)
    Short name:
    AtMPK18
    Short name:
    MAP kinase 18
    Gene namesi
    Name:MPK18
    Ordered Locus Names:At1g53510
    ORF Names:F22G10.12, T3F20.17
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G53510.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth condition, but mutant plants show reduced sensitivity to microtubule-disrupting drugs.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 615615Mitogen-activated protein kinase 18PRO_0000245818Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei187 – 1871PhosphothreonineBy similarity
    Modified residuei189 – 1891PhosphotyrosineBy similarity
    Modified residuei192 – 1921PhosphothreonineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-187 and Tyr-189, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9C5C0.
    PRIDEiQ9C5C0.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Gene expression databases

    GenevestigatoriQ9C5C0.

    Interactioni

    Subunit structurei

    Interacts with PHS1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PHS1Q75QN63EBI-1238534,EBI-2349366

    Protein-protein interaction databases

    BioGridi27011. 6 interactions.
    IntActiQ9C5C0. 19 interactions.
    STRINGi3702.AT1G53510.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C5C0.
    SMRiQ9C5C0. Positions 18-384.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 316292Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi187 – 1893TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    InParanoidiQ9C5C0.
    OMAiNTHMAID.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9C5C0-1 [UniParc]FASTAAdd to Basket

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    MQQNQVKKGT KEMEFFTEYG DANRYRILEV IGKGSYGVVC AAIDTHTGEK    50
    VAIKKINDVF EHISDALRIL REVKLLRLLR HPDIVEIKSI MLPPSKREFK 100
    DIYVVFELME SDLHQVIKAN DDLTREHHQF FLYQMLRALK FMHTANVYHR 150
    DLKPKNILAN ANCKLKVCDF GLARVAFNDT PTTVFWTDYV ATRWYRAPEL 200
    CGSFFSKYTP AIDVWSIGCI FAEVLTGKPL FPGKSVVHQL ELITDLLGTP 250
    KSETISGVRN DKARKYLTEM RKKNPVTFSQ KFSKADPLAL RLLQRLLAFD 300
    PKDRPTPAEA LADPYFKGLS KIEREPSSQQ ISKMEFEFER RRLTKDDIRE 350
    LIYREILEYH PQLLKDYMSG SEGSNFVYPS AIGHLRQQFT YLEENSSRNG 400
    PVIPLERKHA SLPRSTVHST VVHSTSQPNL GATDSRRVSF EPSKNGASSA 450
    GHPSTSAYPT KSIGPPPRVP PSGRPGRVVE SSVSYENGRN LKEAYFRSAV 500
    SSPHCYFRPN TMTNPENRNI EASSFPPKPQ NPVHQFSPTE PPAATTNQAD 550
    VETMNHPNPY FQPQLPKTDQ LNNNTHMAID AKLLQAQSQF GPAGAAAVAV 600
    AAHRNIGTIS YSAAS 615
    Length:615
    Mass (Da):69,352
    Last modified:May 31, 2011 - v4
    Checksum:i1439ED0B3C82210E
    GO

    Sequence cautioni

    The sequence AAF78438.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence AAG51978.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC018748 Genomic DNA. Translation: AAF78438.1. Sequence problems.
    AC024260 Genomic DNA. Translation: AAG51978.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE32950.1.
    AF360353 mRNA. Translation: AAK28649.2.
    BT000870 mRNA. Translation: AAN41270.1.
    PIRiC96575.
    RefSeqiNP_175756.2. NM_104229.3.
    UniGeneiAt.25395.

    Genome annotation databases

    EnsemblPlantsiAT1G53510.1; AT1G53510.1; AT1G53510.
    GeneIDi841786.
    KEGGiath:AT1G53510.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC018748 Genomic DNA. Translation: AAF78438.1 . Sequence problems.
    AC024260 Genomic DNA. Translation: AAG51978.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE32950.1 .
    AF360353 mRNA. Translation: AAK28649.2 .
    BT000870 mRNA. Translation: AAN41270.1 .
    PIRi C96575.
    RefSeqi NP_175756.2. NM_104229.3.
    UniGenei At.25395.

    3D structure databases

    ProteinModelPortali Q9C5C0.
    SMRi Q9C5C0. Positions 18-384.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 27011. 6 interactions.
    IntActi Q9C5C0. 19 interactions.
    STRINGi 3702.AT1G53510.1-P.

    Proteomic databases

    PaxDbi Q9C5C0.
    PRIDEi Q9C5C0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G53510.1 ; AT1G53510.1 ; AT1G53510 .
    GeneIDi 841786.
    KEGGi ath:AT1G53510.

    Organism-specific databases

    GeneFarmi 857. 89.
    TAIRi AT1G53510.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    InParanoidi Q9C5C0.
    OMAi NTHMAID.

    Enzyme and pathway databases

    BioCyci ARA:AT1G53510-MONOMER.

    Miscellaneous databases

    PROi Q9C5C0.

    Gene expression databases

    Genevestigatori Q9C5C0.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 13-615.
      Strain: cv. Columbia.
    4. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
      MAPK group
      Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. Cited for: GENE FAMILY.
    6. "Arabidopsis mitogen-activated protein kinase MPK18 mediates cortical microtubule functions in plant cells."
      Walia A., Lee J.S., Wasteneys G., Ellis B.
      Plant J. 59:565-575(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PHS1, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiMPK18_ARATH
    AccessioniPrimary (citable) accession number: Q9C5C0
    Secondary accession number(s): Q9LPG7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: May 31, 2011
    Last modified: October 1, 2014
    This is version 85 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3