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Protein

Mitogen-activated protein kinase 18

Gene

MPK18

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitogen-activated protein kinase (MAPK) that is specifically regulated by PHS1 and mediates signaling that regulates cortical microtubule functions, maybe through regulation of microtubule dynamic instability.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation (Probable). Inactivated by phosphatase PHS1.Curated1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATPPROSITE-ProRule annotation
Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: TAIR

GO - Biological processi

  1. cortical microtubule organization Source: TAIR
  2. MAPK cascade Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G53510-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 18 (EC:2.7.11.24)
Short name:
AtMPK18
Short name:
MAP kinase 18
Gene namesi
Name:MPK18
Ordered Locus Names:At1g53510
ORF Names:F22G10.12, T3F20.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G53510.

Subcellular locationi

  1. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth condition, but mutant plants show reduced sensitivity to microtubule-disrupting drugs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 615615Mitogen-activated protein kinase 18PRO_0000245818Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei187 – 1871PhosphothreonineBy similarity
Modified residuei189 – 1891PhosphotyrosineBy similarity
Modified residuei192 – 1921PhosphothreonineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-187 and Tyr-189, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9C5C0.
PRIDEiQ9C5C0.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

GenevestigatoriQ9C5C0.

Interactioni

Subunit structurei

Interacts with PHS1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PHS1Q75QN63EBI-1238534,EBI-2349366

Protein-protein interaction databases

BioGridi27011. 6 interactions.
IntActiQ9C5C0. 19 interactions.
STRINGi3702.AT1G53510.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9C5C0.
SMRiQ9C5C0. Positions 18-384.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 316292Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi187 – 1893TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ9C5C0.
OMAiNTHMAID.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9C5C0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQNQVKKGT KEMEFFTEYG DANRYRILEV IGKGSYGVVC AAIDTHTGEK
60 70 80 90 100
VAIKKINDVF EHISDALRIL REVKLLRLLR HPDIVEIKSI MLPPSKREFK
110 120 130 140 150
DIYVVFELME SDLHQVIKAN DDLTREHHQF FLYQMLRALK FMHTANVYHR
160 170 180 190 200
DLKPKNILAN ANCKLKVCDF GLARVAFNDT PTTVFWTDYV ATRWYRAPEL
210 220 230 240 250
CGSFFSKYTP AIDVWSIGCI FAEVLTGKPL FPGKSVVHQL ELITDLLGTP
260 270 280 290 300
KSETISGVRN DKARKYLTEM RKKNPVTFSQ KFSKADPLAL RLLQRLLAFD
310 320 330 340 350
PKDRPTPAEA LADPYFKGLS KIEREPSSQQ ISKMEFEFER RRLTKDDIRE
360 370 380 390 400
LIYREILEYH PQLLKDYMSG SEGSNFVYPS AIGHLRQQFT YLEENSSRNG
410 420 430 440 450
PVIPLERKHA SLPRSTVHST VVHSTSQPNL GATDSRRVSF EPSKNGASSA
460 470 480 490 500
GHPSTSAYPT KSIGPPPRVP PSGRPGRVVE SSVSYENGRN LKEAYFRSAV
510 520 530 540 550
SSPHCYFRPN TMTNPENRNI EASSFPPKPQ NPVHQFSPTE PPAATTNQAD
560 570 580 590 600
VETMNHPNPY FQPQLPKTDQ LNNNTHMAID AKLLQAQSQF GPAGAAAVAV
610
AAHRNIGTIS YSAAS
Length:615
Mass (Da):69,352
Last modified:May 31, 2011 - v4
Checksum:i1439ED0B3C82210E
GO

Sequence cautioni

The sequence AAF78438.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAG51978.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC018748 Genomic DNA. Translation: AAF78438.1. Sequence problems.
AC024260 Genomic DNA. Translation: AAG51978.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32950.1.
AF360353 mRNA. Translation: AAK28649.2.
BT000870 mRNA. Translation: AAN41270.1.
PIRiC96575.
RefSeqiNP_175756.2. NM_104229.3.
UniGeneiAt.25395.

Genome annotation databases

EnsemblPlantsiAT1G53510.1; AT1G53510.1; AT1G53510.
GeneIDi841786.
KEGGiath:AT1G53510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC018748 Genomic DNA. Translation: AAF78438.1. Sequence problems.
AC024260 Genomic DNA. Translation: AAG51978.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32950.1.
AF360353 mRNA. Translation: AAK28649.2.
BT000870 mRNA. Translation: AAN41270.1.
PIRiC96575.
RefSeqiNP_175756.2. NM_104229.3.
UniGeneiAt.25395.

3D structure databases

ProteinModelPortaliQ9C5C0.
SMRiQ9C5C0. Positions 18-384.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27011. 6 interactions.
IntActiQ9C5C0. 19 interactions.
STRINGi3702.AT1G53510.1-P.

Proteomic databases

PaxDbiQ9C5C0.
PRIDEiQ9C5C0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G53510.1; AT1G53510.1; AT1G53510.
GeneIDi841786.
KEGGiath:AT1G53510.

Organism-specific databases

GeneFarmi857. 89.
TAIRiAT1G53510.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ9C5C0.
OMAiNTHMAID.

Enzyme and pathway databases

BioCyciARA:AT1G53510-MONOMER.

Miscellaneous databases

PROiQ9C5C0.

Gene expression databases

GenevestigatoriQ9C5C0.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 13-615.
    Strain: cv. Columbia.
  4. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. Cited for: GENE FAMILY.
  6. "Arabidopsis mitogen-activated protein kinase MPK18 mediates cortical microtubule functions in plant cells."
    Walia A., Lee J.S., Wasteneys G., Ellis B.
    Plant J. 59:565-575(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PHS1, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMPK18_ARATH
AccessioniPrimary (citable) accession number: Q9C5C0
Secondary accession number(s): Q9LPG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: May 31, 2011
Last modified: March 4, 2015
This is version 88 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.