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Protein

Mitogen-activated protein kinase 18

Gene

MPK18

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitogen-activated protein kinase (MAPK) that is specifically regulated by PHS1 and mediates signaling that regulates cortical microtubule functions, maybe through regulation of microtubule dynamic instability.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation (Probable). Inactivated by phosphatase PHS1.Curated1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54ATPPROSITE-ProRule annotation1
Active sitei151Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR

GO - Biological processi

  • cortical microtubule organization Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 18 (EC:2.7.11.24)
Short name:
AtMPK18
Short name:
MAP kinase 18
Gene namesi
Name:MPK18
Ordered Locus Names:At1g53510
ORF Names:F22G10.12, T3F20.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G53510.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytoplasm Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth condition, but mutant plants show reduced sensitivity to microtubule-disrupting drugs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002458181 – 615Mitogen-activated protein kinase 18Add BLAST615

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei187PhosphothreonineBy similarity1
Modified residuei189PhosphotyrosineBy similarity1
Modified residuei192PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-187 and Tyr-189, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9C5C0.

PTM databases

iPTMnetiQ9C5C0.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

GenevisibleiQ9C5C0. AT.

Interactioni

Subunit structurei

Interacts with PHS1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PHS1Q75QN63EBI-1238534,EBI-2349366

Protein-protein interaction databases

BioGridi27011. 8 interactors.
IntActiQ9C5C0. 19 interactors.
STRINGi3702.AT1G53510.1.

Structurei

3D structure databases

ProteinModelPortaliQ9C5C0.
SMRiQ9C5C0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 316Protein kinasePROSITE-ProRule annotationAdd BLAST292

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi187 – 189TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ9C5C0.
OMAiNTHMAID.
OrthoDBiEOG093605CF.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9C5C0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQNQVKKGT KEMEFFTEYG DANRYRILEV IGKGSYGVVC AAIDTHTGEK
60 70 80 90 100
VAIKKINDVF EHISDALRIL REVKLLRLLR HPDIVEIKSI MLPPSKREFK
110 120 130 140 150
DIYVVFELME SDLHQVIKAN DDLTREHHQF FLYQMLRALK FMHTANVYHR
160 170 180 190 200
DLKPKNILAN ANCKLKVCDF GLARVAFNDT PTTVFWTDYV ATRWYRAPEL
210 220 230 240 250
CGSFFSKYTP AIDVWSIGCI FAEVLTGKPL FPGKSVVHQL ELITDLLGTP
260 270 280 290 300
KSETISGVRN DKARKYLTEM RKKNPVTFSQ KFSKADPLAL RLLQRLLAFD
310 320 330 340 350
PKDRPTPAEA LADPYFKGLS KIEREPSSQQ ISKMEFEFER RRLTKDDIRE
360 370 380 390 400
LIYREILEYH PQLLKDYMSG SEGSNFVYPS AIGHLRQQFT YLEENSSRNG
410 420 430 440 450
PVIPLERKHA SLPRSTVHST VVHSTSQPNL GATDSRRVSF EPSKNGASSA
460 470 480 490 500
GHPSTSAYPT KSIGPPPRVP PSGRPGRVVE SSVSYENGRN LKEAYFRSAV
510 520 530 540 550
SSPHCYFRPN TMTNPENRNI EASSFPPKPQ NPVHQFSPTE PPAATTNQAD
560 570 580 590 600
VETMNHPNPY FQPQLPKTDQ LNNNTHMAID AKLLQAQSQF GPAGAAAVAV
610
AAHRNIGTIS YSAAS
Length:615
Mass (Da):69,352
Last modified:May 31, 2011 - v4
Checksum:i1439ED0B3C82210E
GO

Sequence cautioni

The sequence AAF78438 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG51978 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC018748 Genomic DNA. Translation: AAF78438.1. Sequence problems.
AC024260 Genomic DNA. Translation: AAG51978.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32950.1.
AF360353 mRNA. Translation: AAK28649.2.
BT000870 mRNA. Translation: AAN41270.1.
PIRiC96575.
RefSeqiNP_175756.2. NM_104229.4.
UniGeneiAt.25395.

Genome annotation databases

EnsemblPlantsiAT1G53510.1; AT1G53510.1; AT1G53510.
GeneIDi841786.
GrameneiAT1G53510.1; AT1G53510.1; AT1G53510.
KEGGiath:AT1G53510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC018748 Genomic DNA. Translation: AAF78438.1. Sequence problems.
AC024260 Genomic DNA. Translation: AAG51978.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32950.1.
AF360353 mRNA. Translation: AAK28649.2.
BT000870 mRNA. Translation: AAN41270.1.
PIRiC96575.
RefSeqiNP_175756.2. NM_104229.4.
UniGeneiAt.25395.

3D structure databases

ProteinModelPortaliQ9C5C0.
SMRiQ9C5C0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27011. 8 interactors.
IntActiQ9C5C0. 19 interactors.
STRINGi3702.AT1G53510.1.

PTM databases

iPTMnetiQ9C5C0.

Proteomic databases

PaxDbiQ9C5C0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G53510.1; AT1G53510.1; AT1G53510.
GeneIDi841786.
GrameneiAT1G53510.1; AT1G53510.1; AT1G53510.
KEGGiath:AT1G53510.

Organism-specific databases

TAIRiAT1G53510.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ9C5C0.
OMAiNTHMAID.
OrthoDBiEOG093605CF.

Miscellaneous databases

PROiQ9C5C0.

Gene expression databases

GenevisibleiQ9C5C0. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPK18_ARATH
AccessioniPrimary (citable) accession number: Q9C5C0
Secondary accession number(s): Q9LPG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: May 31, 2011
Last modified: November 30, 2016
This is version 102 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.