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Q9C5C0

- MPK18_ARATH

UniProt

Q9C5C0 - MPK18_ARATH

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Protein
Mitogen-activated protein kinase 18
Gene
MPK18, At1g53510, F22G10.12, T3F20.17
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mitogen-activated protein kinase (MAPK) that is specifically regulated by PHS1 and mediates signaling that regulates cortical microtubule functions, maybe through regulation of microtubule dynamic instability.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation Inferred. Inactivated by phosphatase PHS1.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATP By similarity
Active sitei151 – 1511Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 399ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: TAIR
  3. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. MAPK cascade Source: GOC
  2. cortical microtubule organization Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G53510-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 18 (EC:2.7.11.24)
Short name:
AtMPK18
Short name:
MAP kinase 18
Gene namesi
Name:MPK18
Ordered Locus Names:At1g53510
ORF Names:F22G10.12, T3F20.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G53510.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth condition, but mutant plants show reduced sensitivity to microtubule-disrupting drugs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 615615Mitogen-activated protein kinase 18
PRO_0000245818Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei187 – 1871Phosphothreonine By similarity
Modified residuei189 – 1891Phosphotyrosine By similarity
Modified residuei192 – 1921Phosphothreonine By similarity

Post-translational modificationi

Dually phosphorylated on Thr-187 and Tyr-189, which activates the enzyme By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9C5C0.
PRIDEiQ9C5C0.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

GenevestigatoriQ9C5C0.

Interactioni

Subunit structurei

Interacts with PHS1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PHS1Q75QN63EBI-1238534,EBI-2349366

Protein-protein interaction databases

BioGridi27011. 6 interactions.
IntActiQ9C5C0. 19 interactions.
STRINGi3702.AT1G53510.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9C5C0.
SMRiQ9C5C0. Positions 18-384.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 316292Protein kinase
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi187 – 1893TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ9C5C0.
OMAiNTHMAID.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9C5C0-1 [UniParc]FASTAAdd to Basket

« Hide

MQQNQVKKGT KEMEFFTEYG DANRYRILEV IGKGSYGVVC AAIDTHTGEK    50
VAIKKINDVF EHISDALRIL REVKLLRLLR HPDIVEIKSI MLPPSKREFK 100
DIYVVFELME SDLHQVIKAN DDLTREHHQF FLYQMLRALK FMHTANVYHR 150
DLKPKNILAN ANCKLKVCDF GLARVAFNDT PTTVFWTDYV ATRWYRAPEL 200
CGSFFSKYTP AIDVWSIGCI FAEVLTGKPL FPGKSVVHQL ELITDLLGTP 250
KSETISGVRN DKARKYLTEM RKKNPVTFSQ KFSKADPLAL RLLQRLLAFD 300
PKDRPTPAEA LADPYFKGLS KIEREPSSQQ ISKMEFEFER RRLTKDDIRE 350
LIYREILEYH PQLLKDYMSG SEGSNFVYPS AIGHLRQQFT YLEENSSRNG 400
PVIPLERKHA SLPRSTVHST VVHSTSQPNL GATDSRRVSF EPSKNGASSA 450
GHPSTSAYPT KSIGPPPRVP PSGRPGRVVE SSVSYENGRN LKEAYFRSAV 500
SSPHCYFRPN TMTNPENRNI EASSFPPKPQ NPVHQFSPTE PPAATTNQAD 550
VETMNHPNPY FQPQLPKTDQ LNNNTHMAID AKLLQAQSQF GPAGAAAVAV 600
AAHRNIGTIS YSAAS 615
Length:615
Mass (Da):69,352
Last modified:May 31, 2011 - v4
Checksum:i1439ED0B3C82210E
GO

Sequence cautioni

The sequence AAF78438.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence AAG51978.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC018748 Genomic DNA. Translation: AAF78438.1. Sequence problems.
AC024260 Genomic DNA. Translation: AAG51978.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32950.1.
AF360353 mRNA. Translation: AAK28649.2.
BT000870 mRNA. Translation: AAN41270.1.
PIRiC96575.
RefSeqiNP_175756.2. NM_104229.3.
UniGeneiAt.25395.

Genome annotation databases

EnsemblPlantsiAT1G53510.1; AT1G53510.1; AT1G53510.
GeneIDi841786.
KEGGiath:AT1G53510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC018748 Genomic DNA. Translation: AAF78438.1 . Sequence problems.
AC024260 Genomic DNA. Translation: AAG51978.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE32950.1 .
AF360353 mRNA. Translation: AAK28649.2 .
BT000870 mRNA. Translation: AAN41270.1 .
PIRi C96575.
RefSeqi NP_175756.2. NM_104229.3.
UniGenei At.25395.

3D structure databases

ProteinModelPortali Q9C5C0.
SMRi Q9C5C0. Positions 18-384.
ModBasei Search...

Protein-protein interaction databases

BioGridi 27011. 6 interactions.
IntActi Q9C5C0. 19 interactions.
STRINGi 3702.AT1G53510.1-P.

Proteomic databases

PaxDbi Q9C5C0.
PRIDEi Q9C5C0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G53510.1 ; AT1G53510.1 ; AT1G53510 .
GeneIDi 841786.
KEGGi ath:AT1G53510.

Organism-specific databases

GeneFarmi 857. 89.
TAIRi AT1G53510.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi Q9C5C0.
OMAi NTHMAID.

Enzyme and pathway databases

BioCyci ARA:AT1G53510-MONOMER.

Miscellaneous databases

PROi Q9C5C0.

Gene expression databases

Genevestigatori Q9C5C0.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 13-615.
    Strain: cv. Columbia.
  4. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. Cited for: GENE FAMILY.
  6. "Arabidopsis mitogen-activated protein kinase MPK18 mediates cortical microtubule functions in plant cells."
    Walia A., Lee J.S., Wasteneys G., Ellis B.
    Plant J. 59:565-575(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PHS1, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMPK18_ARATH
AccessioniPrimary (citable) accession number: Q9C5C0
Secondary accession number(s): Q9LPG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: May 31, 2011
Last modified: June 11, 2014
This is version 84 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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