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Q9C585 (UBP8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 8

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 8
Short name=AtUBP8
Ubiquitin thioesterase 8
Ubiquitin-specific-processing protease 8
Gene names
Name:UBP8
Ordered Locus Names:At5g22030
ORF Names:T6G21.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length871 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 DUSP domain.

Contains 1 USP domain.

Sequence caution

The sequence AED92970.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AED92971.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BAE98798.1 differs from that shown. Reason: Probable cloning artifact leading to an insertion into the sequence.

The sequence CAC34496.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 871871Ubiquitin carboxyl-terminal hydrolase 8
PRO_0000313035

Regions

Domain4 – 9996DUSP
Domain279 – 869591USP

Sites

Active site2881Nucleophile By similarity
Active site8281Proton acceptor By similarity

Experimental info

Sequence conflict4141N → P in AAG42753. Ref.4
Sequence conflict6181E → K in BAE98798. Ref.3
Sequence conflict7031L → F in AAG42753. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9C585 [UniParc].

Last modified September 1, 2009. Version 2.
Checksum: A669206C1AA3C5F6

FASTA87198,171
        10         20         30         40         50         60 
MNGTSPDESP DSTTQRIDSF NGEQRVYFVP LRWWKDAQDS MPSESVEKRE ILYTASCGSS 

        70         80         90        100        110        120 
YGGPMKLINN IFNSDILFDL RREGDALQNG ETGEASVSGR DFALVSSDMW LQALKWYHDD 

       130        140        150        160        170        180 
KNTEKGVKSF SAGGVDRGDV YPVQLRLSVL QETNSLAVKI CKKDNSVECF RRACKIFSLD 

       190        200        210        220        230        240 
SEQLRIWDIS GQTTLFFESD VSNSKDCQQQ ADQEILLELQ IYGLSDSIKL KESKKEDGST 

       250        260        270        280        290        300 
QQTNGITNGM NGGTVFRFGR SNSLSFLGKA GEAGTLGLTG LQNLGNTCFM NSSLQCLAHT 

       310        320        330        340        350        360 
PKLVDFFLGE YSKEINLDNP LGMKGEIALA FGDLLRSLWA PGASTVAPRT FKAKLARFAP 

       370        380        390        400        410        420 
QFSGFNQHDS QELLAFLLDG LHEDLNRVKN KPYVEAKDGD GRPDAEVADE YWRNHVARND 

       430        440        450        460        470        480 
SIIVDVCQGQ YKSTLVCPIC KKVSVMFDPF MYLSLPLPCT SMRTMDLTVM SADGSSLPIP 

       490        500        510        520        530        540 
LTVNVPKFGK FEDLHKALVT ACSLPEEETL LVTEVYNNRI IRFLEEPTDS LTLIRDGDKL 

       550        560        570        580        590        600 
VVYRLKKDAN NSPLIVYMHQ KLEEQFISGK SSPTWKAFGI PLVSRLCDVE NGSDVENLYL 

       610        620        630        640        650        660 
KLLSSFKMPT EFFTENLENP TEEEATDKTD TDGTTSVEDT NSTDVKETTE SLPDPVLRLY 

       670        680        690        700        710        720 
LTDDRGNSIE AEMLKEKPVN KSKRLNVLAR WPVKELDVYD TCLLSSLPEV SKSGTKRPQE 

       730        740        750        760        770        780 
SVSLFKCLEA FLTEEPLGPD DMWYCPGCKE HRQAIKKLDL WRLPEILVIH LKRFSYSRFM 

       790        800        810        820        830        840 
KNKLEAYVDF PLDNLDLSSY ISYKNGQTTY RYMLYAISNH YGSMGGGHYT AYVHHGGDRW 

       850        860        870 
YDFDDSHVHQ ISQEKIKTSA AYVLFYKRLV D 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 402-871, GENE FAMILY ORGANIZATION, NOMENCLATURE.
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL589883 Genomic DNA. Translation: CAC34496.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92970.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92971.1. Sequence problems.
AK226691 mRNA. Translation: BAE98798.1. Sequence problems.
AF302662 mRNA. Translation: AAG42753.1.
RefSeqNP_568411.4. NM_122126.5.
NP_851052.4. NM_180721.4.
UniGeneAt.23027.

3D structure databases

ProteinModelPortalQ9C585.
SMRQ9C585. Positions 277-505, 721-871.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC19.A01.

Proteomic databases

PaxDbQ9C585.
PRIDEQ9C585.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID832263.
KEGGath:AT5G22030.

Organism-specific databases

TAIRAT5G22030.

Phylogenomic databases

eggNOGCOG5560.
InParanoidQ9C585.
KOK11835.
PhylomeDBQ9C585.

Enzyme and pathway databases

BioCycARA:AT5G22030-MONOMER.
ARA:GQT-1231-MONOMER.

Gene expression databases

GenevestigatorQ9C585.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP8_ARATH
AccessionPrimary (citable) accession number: Q9C585
Secondary accession number(s): F4K8E0, Q0WVQ0, Q9FPT2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 1, 2009
Last modified: April 16, 2014
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names