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Q9C585

- UBP8_ARATH

UniProt

Q9C585 - UBP8_ARATH

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Protein

Ubiquitin carboxyl-terminal hydrolase 8

Gene

UBP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei288 – 2881NucleophilePROSITE-ProRule annotation
Active sitei828 – 8281Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciARA:AT5G22030-MONOMER.
ARA:GQT-1231-MONOMER.

Protein family/group databases

MEROPSiC19.A01.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 8 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 8
Short name:
AtUBP8
Ubiquitin thioesterase 8
Ubiquitin-specific-processing protease 8
Gene namesi
Name:UBP8
Ordered Locus Names:At5g22030
ORF Names:T6G21.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G22030.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 871871Ubiquitin carboxyl-terminal hydrolase 8PRO_0000313035Add
BLAST

Proteomic databases

PaxDbiQ9C585.
PRIDEiQ9C585.

Expressioni

Gene expression databases

GenevestigatoriQ9C585.

Structurei

3D structure databases

ProteinModelPortaliQ9C585.
SMRiQ9C585. Positions 277-505, 721-871.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 9996DUSPAdd
BLAST
Domaini279 – 869591USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 DUSP domain.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5560.
InParanoidiQ9C585.
KOiK11835.
PhylomeDBiQ9C585.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9C585-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNGTSPDESP DSTTQRIDSF NGEQRVYFVP LRWWKDAQDS MPSESVEKRE
60 70 80 90 100
ILYTASCGSS YGGPMKLINN IFNSDILFDL RREGDALQNG ETGEASVSGR
110 120 130 140 150
DFALVSSDMW LQALKWYHDD KNTEKGVKSF SAGGVDRGDV YPVQLRLSVL
160 170 180 190 200
QETNSLAVKI CKKDNSVECF RRACKIFSLD SEQLRIWDIS GQTTLFFESD
210 220 230 240 250
VSNSKDCQQQ ADQEILLELQ IYGLSDSIKL KESKKEDGST QQTNGITNGM
260 270 280 290 300
NGGTVFRFGR SNSLSFLGKA GEAGTLGLTG LQNLGNTCFM NSSLQCLAHT
310 320 330 340 350
PKLVDFFLGE YSKEINLDNP LGMKGEIALA FGDLLRSLWA PGASTVAPRT
360 370 380 390 400
FKAKLARFAP QFSGFNQHDS QELLAFLLDG LHEDLNRVKN KPYVEAKDGD
410 420 430 440 450
GRPDAEVADE YWRNHVARND SIIVDVCQGQ YKSTLVCPIC KKVSVMFDPF
460 470 480 490 500
MYLSLPLPCT SMRTMDLTVM SADGSSLPIP LTVNVPKFGK FEDLHKALVT
510 520 530 540 550
ACSLPEEETL LVTEVYNNRI IRFLEEPTDS LTLIRDGDKL VVYRLKKDAN
560 570 580 590 600
NSPLIVYMHQ KLEEQFISGK SSPTWKAFGI PLVSRLCDVE NGSDVENLYL
610 620 630 640 650
KLLSSFKMPT EFFTENLENP TEEEATDKTD TDGTTSVEDT NSTDVKETTE
660 670 680 690 700
SLPDPVLRLY LTDDRGNSIE AEMLKEKPVN KSKRLNVLAR WPVKELDVYD
710 720 730 740 750
TCLLSSLPEV SKSGTKRPQE SVSLFKCLEA FLTEEPLGPD DMWYCPGCKE
760 770 780 790 800
HRQAIKKLDL WRLPEILVIH LKRFSYSRFM KNKLEAYVDF PLDNLDLSSY
810 820 830 840 850
ISYKNGQTTY RYMLYAISNH YGSMGGGHYT AYVHHGGDRW YDFDDSHVHQ
860 870
ISQEKIKTSA AYVLFYKRLV D
Length:871
Mass (Da):98,171
Last modified:September 1, 2009 - v2
Checksum:iA669206C1AA3C5F6
GO

Sequence cautioni

The sequence AED92970.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AED92971.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence BAE98798.1 differs from that shown. Reason: Probable cloning artifact leading to an insertion into the sequence.Curated
The sequence CAC34496.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti414 – 4141N → P in AAG42753. (PubMed:11115897)Curated
Sequence conflicti618 – 6181E → K in BAE98798. 1 PublicationCurated
Sequence conflicti703 – 7031L → F in AAG42753. (PubMed:11115897)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL589883 Genomic DNA. Translation: CAC34496.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92970.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92971.1. Sequence problems.
AK226691 mRNA. Translation: BAE98798.1. Sequence problems.
AF302662 mRNA. Translation: AAG42753.1.
RefSeqiNP_568411.4. NM_122126.5.
NP_851052.4. NM_180721.4.
UniGeneiAt.23027.

Genome annotation databases

GeneIDi832263.
KEGGiath:AT5G22030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL589883 Genomic DNA. Translation: CAC34496.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED92970.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED92971.1 . Sequence problems.
AK226691 mRNA. Translation: BAE98798.1 . Sequence problems.
AF302662 mRNA. Translation: AAG42753.1 .
RefSeqi NP_568411.4. NM_122126.5.
NP_851052.4. NM_180721.4.
UniGenei At.23027.

3D structure databases

ProteinModelPortali Q9C585.
SMRi Q9C585. Positions 277-505, 721-871.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C19.A01.

Proteomic databases

PaxDbi Q9C585.
PRIDEi Q9C585.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 832263.
KEGGi ath:AT5G22030.

Organism-specific databases

TAIRi AT5G22030.

Phylogenomic databases

eggNOGi COG5560.
InParanoidi Q9C585.
KOi K11835.
PhylomeDBi Q9C585.

Enzyme and pathway databases

BioCyci ARA:AT5G22030-MONOMER.
ARA:GQT-1231-MONOMER.

Gene expression databases

Genevestigatori Q9C585.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 402-871, GENE FAMILY ORGANIZATION, NOMENCLATURE.
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBP8_ARATH
AccessioniPrimary (citable) accession number: Q9C585
Secondary accession number(s): F4K8E0, Q0WVQ0, Q9FPT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 1, 2009
Last modified: October 1, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3