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Protein

Ubiquitin carboxyl-terminal hydrolase 8

Gene

UBP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei288NucleophilePROSITE-ProRule annotation1
Active sitei828Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Protein family/group databases

MEROPSiC19.A01

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 8 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 8
Short name:
AtUBP8
Ubiquitin thioesterase 8
Ubiquitin-specific-processing protease 8
Gene namesi
Name:UBP8
Ordered Locus Names:At5g22030
ORF Names:T6G21.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G22030
TAIRilocus:505006627 AT5G22030

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003130351 – 871Ubiquitin carboxyl-terminal hydrolase 8Add BLAST871

Proteomic databases

PRIDEiQ9C585

PTM databases

iPTMnetiQ9C585

Expressioni

Gene expression databases

ExpressionAtlasiQ9C585 baseline and differential

Structurei

3D structure databases

ProteinModelPortaliQ9C585
SMRiQ9C585
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 99DUSPAdd BLAST96
Domaini279 – 869USPAdd BLAST591

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Phylogenomic databases

InParanoidiQ9C585
PhylomeDBiQ9C585

Family and domain databases

InterProiView protein in InterPro
IPR001394 Peptidase_C19_UCH
IPR028134 USP4
IPR018200 USP_CS
IPR028889 USP_dom
PANTHERiPTHR43913 PTHR43913, 1 hit
PfamiView protein in Pfam
PF00443 UCH, 1 hit
PROSITEiView protein in PROSITE
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q9C585-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGTSPDESP DSTTQRIDSF NGEQRVYFVP LRWWKDAQDS MPSESVEKRE
60 70 80 90 100
ILYTASCGSS YGGPMKLINN IFNSDILFDL RREGDALQNG ETGEASVSGR
110 120 130 140 150
DFALVSSDMW LQALKWYHDD KNTEKGVKSF SAGGVDRGDV YPVQLRLSVL
160 170 180 190 200
QETNSLAVKI CKKDNSVECF RRACKIFSLD SEQLRIWDIS GQTTLFFESD
210 220 230 240 250
VSNSKDCQQQ ADQEILLELQ IYGLSDSIKL KESKKEDGST QQTNGITNGM
260 270 280 290 300
NGGTVFRFGR SNSLSFLGKA GEAGTLGLTG LQNLGNTCFM NSSLQCLAHT
310 320 330 340 350
PKLVDFFLGE YSKEINLDNP LGMKGEIALA FGDLLRSLWA PGASTVAPRT
360 370 380 390 400
FKAKLARFAP QFSGFNQHDS QELLAFLLDG LHEDLNRVKN KPYVEAKDGD
410 420 430 440 450
GRPDAEVADE YWRNHVARND SIIVDVCQGQ YKSTLVCPIC KKVSVMFDPF
460 470 480 490 500
MYLSLPLPCT SMRTMDLTVM SADGSSLPIP LTVNVPKFGK FEDLHKALVT
510 520 530 540 550
ACSLPEEETL LVTEVYNNRI IRFLEEPTDS LTLIRDGDKL VVYRLKKDAN
560 570 580 590 600
NSPLIVYMHQ KLEEQFISGK SSPTWKAFGI PLVSRLCDVE NGSDVENLYL
610 620 630 640 650
KLLSSFKMPT EFFTENLENP TEEEATDKTD TDGTTSVEDT NSTDVKETTE
660 670 680 690 700
SLPDPVLRLY LTDDRGNSIE AEMLKEKPVN KSKRLNVLAR WPVKELDVYD
710 720 730 740 750
TCLLSSLPEV SKSGTKRPQE SVSLFKCLEA FLTEEPLGPD DMWYCPGCKE
760 770 780 790 800
HRQAIKKLDL WRLPEILVIH LKRFSYSRFM KNKLEAYVDF PLDNLDLSSY
810 820 830 840 850
ISYKNGQTTY RYMLYAISNH YGSMGGGHYT AYVHHGGDRW YDFDDSHVHQ
860 870
ISQEKIKTSA AYVLFYKRLV D
Length:871
Mass (Da):98,171
Last modified:September 1, 2009 - v2
Checksum:iA669206C1AA3C5F6
GO

Sequence cautioni

The sequence AED92970 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAE98798 differs from that shown. Probable cloning artifact leading to an insertion into the sequence.Curated
The sequence CAC34496 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti414N → P in AAG42753 (PubMed:11115897).Curated1
Sequence conflicti618E → K in BAE98798 (Ref. 3) Curated1
Sequence conflicti703L → F in AAG42753 (PubMed:11115897).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL589883 Genomic DNA Translation: CAC34496.1 Sequence problems.
CP002688 Genomic DNA Translation: AED92970.1 Sequence problems.
CP002688 Genomic DNA Translation: AED92971.2
AK226691 mRNA Translation: BAE98798.1 Sequence problems.
AF302662 mRNA Translation: AAG42753.1
RefSeqiNP_001318617.1, NM_001343718.1
NP_568411.4, NM_122126.5
UniGeneiAt.23027

Genome annotation databases

EnsemblPlantsiAT5G22030.1; AT5G22030.1; AT5G22030
GeneIDi832263
GrameneiAT5G22030.1; AT5G22030.1; AT5G22030
KEGGiath:AT5G22030

Similar proteinsi

Entry informationi

Entry nameiUBP8_ARATH
AccessioniPrimary (citable) accession number: Q9C585
Secondary accession number(s): F4K8E0, Q0WVQ0, Q9FPT2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 1, 2009
Last modified: May 23, 2018
This is version 89 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health