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Q9C585

- UBP8_ARATH

UniProt

Q9C585 - UBP8_ARATH

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Protein

Ubiquitin carboxyl-terminal hydrolase 8

Gene
UBP8, At5g22030, T6G21.4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei288 – 2881Nucleophile By similarity
Active sitei828 – 8281Proton acceptor By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciARA:AT5G22030-MONOMER.
ARA:GQT-1231-MONOMER.

Protein family/group databases

MEROPSiC19.A01.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 8 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 8
Short name:
AtUBP8
Ubiquitin thioesterase 8
Ubiquitin-specific-processing protease 8
Gene namesi
Name:UBP8
Ordered Locus Names:At5g22030
ORF Names:T6G21.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G22030.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 871871Ubiquitin carboxyl-terminal hydrolase 8PRO_0000313035Add
BLAST

Proteomic databases

PaxDbiQ9C585.
PRIDEiQ9C585.

Expressioni

Gene expression databases

GenevestigatoriQ9C585.

Structurei

3D structure databases

ProteinModelPortaliQ9C585.
SMRiQ9C585. Positions 277-505, 721-871.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 9996DUSPAdd
BLAST
Domaini279 – 869591USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 1 DUSP domain.
Contains 1 USP domain.

Phylogenomic databases

eggNOGiCOG5560.
InParanoidiQ9C585.
KOiK11835.
PhylomeDBiQ9C585.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9C585-1 [UniParc]FASTAAdd to Basket

« Hide

MNGTSPDESP DSTTQRIDSF NGEQRVYFVP LRWWKDAQDS MPSESVEKRE    50
ILYTASCGSS YGGPMKLINN IFNSDILFDL RREGDALQNG ETGEASVSGR 100
DFALVSSDMW LQALKWYHDD KNTEKGVKSF SAGGVDRGDV YPVQLRLSVL 150
QETNSLAVKI CKKDNSVECF RRACKIFSLD SEQLRIWDIS GQTTLFFESD 200
VSNSKDCQQQ ADQEILLELQ IYGLSDSIKL KESKKEDGST QQTNGITNGM 250
NGGTVFRFGR SNSLSFLGKA GEAGTLGLTG LQNLGNTCFM NSSLQCLAHT 300
PKLVDFFLGE YSKEINLDNP LGMKGEIALA FGDLLRSLWA PGASTVAPRT 350
FKAKLARFAP QFSGFNQHDS QELLAFLLDG LHEDLNRVKN KPYVEAKDGD 400
GRPDAEVADE YWRNHVARND SIIVDVCQGQ YKSTLVCPIC KKVSVMFDPF 450
MYLSLPLPCT SMRTMDLTVM SADGSSLPIP LTVNVPKFGK FEDLHKALVT 500
ACSLPEEETL LVTEVYNNRI IRFLEEPTDS LTLIRDGDKL VVYRLKKDAN 550
NSPLIVYMHQ KLEEQFISGK SSPTWKAFGI PLVSRLCDVE NGSDVENLYL 600
KLLSSFKMPT EFFTENLENP TEEEATDKTD TDGTTSVEDT NSTDVKETTE 650
SLPDPVLRLY LTDDRGNSIE AEMLKEKPVN KSKRLNVLAR WPVKELDVYD 700
TCLLSSLPEV SKSGTKRPQE SVSLFKCLEA FLTEEPLGPD DMWYCPGCKE 750
HRQAIKKLDL WRLPEILVIH LKRFSYSRFM KNKLEAYVDF PLDNLDLSSY 800
ISYKNGQTTY RYMLYAISNH YGSMGGGHYT AYVHHGGDRW YDFDDSHVHQ 850
ISQEKIKTSA AYVLFYKRLV D 871
Length:871
Mass (Da):98,171
Last modified:September 1, 2009 - v2
Checksum:iA669206C1AA3C5F6
GO

Sequence cautioni

The sequence BAE98798.1 differs from that shown. Reason: Probable cloning artifact leading to an insertion into the sequence.
The sequence AED92970.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence AED92971.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAC34496.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti414 – 4141N → P in AAG42753. 1 Publication
Sequence conflicti618 – 6181E → K in BAE98798. 1 Publication
Sequence conflicti703 – 7031L → F in AAG42753. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL589883 Genomic DNA. Translation: CAC34496.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92970.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92971.1. Sequence problems.
AK226691 mRNA. Translation: BAE98798.1. Sequence problems.
AF302662 mRNA. Translation: AAG42753.1.
RefSeqiNP_568411.4. NM_122126.5.
NP_851052.4. NM_180721.4.
UniGeneiAt.23027.

Genome annotation databases

GeneIDi832263.
KEGGiath:AT5G22030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL589883 Genomic DNA. Translation: CAC34496.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED92970.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED92971.1 . Sequence problems.
AK226691 mRNA. Translation: BAE98798.1 . Sequence problems.
AF302662 mRNA. Translation: AAG42753.1 .
RefSeqi NP_568411.4. NM_122126.5.
NP_851052.4. NM_180721.4.
UniGenei At.23027.

3D structure databases

ProteinModelPortali Q9C585.
SMRi Q9C585. Positions 277-505, 721-871.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C19.A01.

Proteomic databases

PaxDbi Q9C585.
PRIDEi Q9C585.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 832263.
KEGGi ath:AT5G22030.

Organism-specific databases

TAIRi AT5G22030.

Phylogenomic databases

eggNOGi COG5560.
InParanoidi Q9C585.
KOi K11835.
PhylomeDBi Q9C585.

Enzyme and pathway databases

BioCyci ARA:AT5G22030-MONOMER.
ARA:GQT-1231-MONOMER.

Gene expression databases

Genevestigatori Q9C585.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 402-871, GENE FAMILY ORGANIZATION, NOMENCLATURE.
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBP8_ARATH
AccessioniPrimary (citable) accession number: Q9C585
Secondary accession number(s): F4K8E0, Q0WVQ0, Q9FPT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 1, 2009
Last modified: September 3, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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