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Q9C585

- UBP8_ARATH

UniProt

Q9C585 - UBP8_ARATH

Protein

Ubiquitin carboxyl-terminal hydrolase 8

Gene

UBP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 2 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei288 – 2881NucleophilePROSITE-ProRule annotation
    Active sitei828 – 8281Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciARA:AT5G22030-MONOMER.
    ARA:GQT-1231-MONOMER.

    Protein family/group databases

    MEROPSiC19.A01.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 8 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 8
    Short name:
    AtUBP8
    Ubiquitin thioesterase 8
    Ubiquitin-specific-processing protease 8
    Gene namesi
    Name:UBP8
    Ordered Locus Names:At5g22030
    ORF Names:T6G21.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G22030.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 871871Ubiquitin carboxyl-terminal hydrolase 8PRO_0000313035Add
    BLAST

    Proteomic databases

    PaxDbiQ9C585.
    PRIDEiQ9C585.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9C585.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C585.
    SMRiQ9C585. Positions 277-505, 721-871.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 9996DUSPAdd
    BLAST
    Domaini279 – 869591USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 DUSP domain.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5560.
    InParanoidiQ9C585.
    KOiK11835.
    PhylomeDBiQ9C585.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9C585-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNGTSPDESP DSTTQRIDSF NGEQRVYFVP LRWWKDAQDS MPSESVEKRE    50
    ILYTASCGSS YGGPMKLINN IFNSDILFDL RREGDALQNG ETGEASVSGR 100
    DFALVSSDMW LQALKWYHDD KNTEKGVKSF SAGGVDRGDV YPVQLRLSVL 150
    QETNSLAVKI CKKDNSVECF RRACKIFSLD SEQLRIWDIS GQTTLFFESD 200
    VSNSKDCQQQ ADQEILLELQ IYGLSDSIKL KESKKEDGST QQTNGITNGM 250
    NGGTVFRFGR SNSLSFLGKA GEAGTLGLTG LQNLGNTCFM NSSLQCLAHT 300
    PKLVDFFLGE YSKEINLDNP LGMKGEIALA FGDLLRSLWA PGASTVAPRT 350
    FKAKLARFAP QFSGFNQHDS QELLAFLLDG LHEDLNRVKN KPYVEAKDGD 400
    GRPDAEVADE YWRNHVARND SIIVDVCQGQ YKSTLVCPIC KKVSVMFDPF 450
    MYLSLPLPCT SMRTMDLTVM SADGSSLPIP LTVNVPKFGK FEDLHKALVT 500
    ACSLPEEETL LVTEVYNNRI IRFLEEPTDS LTLIRDGDKL VVYRLKKDAN 550
    NSPLIVYMHQ KLEEQFISGK SSPTWKAFGI PLVSRLCDVE NGSDVENLYL 600
    KLLSSFKMPT EFFTENLENP TEEEATDKTD TDGTTSVEDT NSTDVKETTE 650
    SLPDPVLRLY LTDDRGNSIE AEMLKEKPVN KSKRLNVLAR WPVKELDVYD 700
    TCLLSSLPEV SKSGTKRPQE SVSLFKCLEA FLTEEPLGPD DMWYCPGCKE 750
    HRQAIKKLDL WRLPEILVIH LKRFSYSRFM KNKLEAYVDF PLDNLDLSSY 800
    ISYKNGQTTY RYMLYAISNH YGSMGGGHYT AYVHHGGDRW YDFDDSHVHQ 850
    ISQEKIKTSA AYVLFYKRLV D 871
    Length:871
    Mass (Da):98,171
    Last modified:September 1, 2009 - v2
    Checksum:iA669206C1AA3C5F6
    GO

    Sequence cautioni

    The sequence BAE98798.1 differs from that shown. Reason: Probable cloning artifact leading to an insertion into the sequence.
    The sequence AED92970.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence AED92971.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAC34496.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti414 – 4141N → P in AAG42753. (PubMed:11115897)Curated
    Sequence conflicti618 – 6181E → K in BAE98798. 1 PublicationCurated
    Sequence conflicti703 – 7031L → F in AAG42753. (PubMed:11115897)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL589883 Genomic DNA. Translation: CAC34496.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED92970.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED92971.1. Sequence problems.
    AK226691 mRNA. Translation: BAE98798.1. Sequence problems.
    AF302662 mRNA. Translation: AAG42753.1.
    RefSeqiNP_568411.4. NM_122126.5.
    NP_851052.4. NM_180721.4.
    UniGeneiAt.23027.

    Genome annotation databases

    GeneIDi832263.
    KEGGiath:AT5G22030.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL589883 Genomic DNA. Translation: CAC34496.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED92970.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED92971.1 . Sequence problems.
    AK226691 mRNA. Translation: BAE98798.1 . Sequence problems.
    AF302662 mRNA. Translation: AAG42753.1 .
    RefSeqi NP_568411.4. NM_122126.5.
    NP_851052.4. NM_180721.4.
    UniGenei At.23027.

    3D structure databases

    ProteinModelPortali Q9C585.
    SMRi Q9C585. Positions 277-505, 721-871.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C19.A01.

    Proteomic databases

    PaxDbi Q9C585.
    PRIDEi Q9C585.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 832263.
    KEGGi ath:AT5G22030.

    Organism-specific databases

    TAIRi AT5G22030.

    Phylogenomic databases

    eggNOGi COG5560.
    InParanoidi Q9C585.
    KOi K11835.
    PhylomeDBi Q9C585.

    Enzyme and pathway databases

    BioCyci ARA:AT5G22030-MONOMER.
    ARA:GQT-1231-MONOMER.

    Gene expression databases

    Genevestigatori Q9C585.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
      Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
      Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 402-871, GENE FAMILY ORGANIZATION, NOMENCLATURE.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUBP8_ARATH
    AccessioniPrimary (citable) accession number: Q9C585
    Secondary accession number(s): F4K8E0, Q0WVQ0, Q9FPT2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 70 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3