Q9C525 (BGL21_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-glucosidase 21
Short name=AtBGLU21
EC=3.2.1.21

Alternative name(s):
Protein PHOSPHATE STARVATION-RESPONSE 3.2

Gene names

Name:	BGLU21
Synonyms:	PSR3.2
Ordered Locus Names:	At1g66270
ORF Names:	T27F4.2, T6J19.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Subcellular location	Endoplasmic reticulum lumen Potential.
Induction	By phosphate starvation in roots. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.
Sequence caution	The sequence AAB64244.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular response to cold Inferred from expression pattern PubMed 19965874. Source: TAIR cellular response to phosphate starvation Inferred from expression pattern Ref.1. Source: UniProtKB cellular response to salt stress Inferred from expression pattern PubMed 19965874. Source: TAIR coumarin metabolic process Inferred from direct assay PubMed 19965874. Source: TAIR
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 22923678. Source: TAIR endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from direct assay PubMed 17432890. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR vacuole Inferred from direct assay PubMed 15539469. Source: TAIR
Molecular_function	beta-glucosidase activity Inferred from direct assay PubMed 19965874. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9C525-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9C525-2)

The sequence of this isoform differs from the canonical sequence as follows:
     149-150: Missing.

Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 524	500	Beta-glucosidase 21		PRO_0000389583
Regions
	Region	475 – 476	2	Substrate binding By similarity
	Motif	521 – 524	4	Prevents secretion from ER Potential
Sites
	Active site	204	1	Proton donor By similarity
	Active site	418	1	Nucleophile By similarity
	Binding site	55	1	Substrate By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	203	1	Substrate By similarity
	Binding site	346	1	Substrate By similarity
	Binding site	468	1	Substrate By similarity
Amino acid modifications
	Glycosylation	61	1	N-linked (GlcNAc...) Potential
	Glycosylation	494	1	N-linked (GlcNAc...) Potential
	Disulfide bond	223 ↔ 230		By similarity
Natural variations
	Alternative sequence	149 – 150	2	Missing in isoform 2.		VSP_038457

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 8848C9751C15DFF9	FASTA	524	59,664
	10 20 30 40 50 60 MALQKFPLMG LLLLLTILVS VTTAVDDPVC PATSKLSRAS FPNGFLFGTA TAAFQVEGAI 70 80 90 100 110 120 NETCRGPALW DIYCRRNPER CSGDHADVAV DFFHRYKEDI QLMKNLNTDA FRLSIAWSRI 130 140 150 160 170 180 FPHGRKEKGV SQAGVQFYHE LIDELLKNGI VPFVTVFHWD TPQDLEDEYG GFLSQNIVKD 190 200 210 220 230 240 FREYADYVFT EYGGKVKNWI TFNEPWVFAH AGYDLGKKAP GRCSRYVPGC EDREGQSGKE 250 260 270 280 290 300 AYLVSHNLLN AHAEAVEVFR QKVKGGKIGI AHSPAWFEPH DLKDSNDAPT VSRVLDFMLG 310 320 330 340 350 360 WHLEPTTSGD YPQIMKDLLG YRLPQFTAAQ KAKLKDSTDF VGLNYYTSTF SNYNEKPDPS 370 380 390 400 410 420 KPSWKQDSLV SWEPKNVDHS AIGSMPLTAA LPVYAKGFRK LLKYIKDKYA NPEIMIMENG 430 440 450 460 470 480 YGDKLGTTDS VDVGTADHNR KYYLQRHLLA MNEAICIDKV RVTGYFVWSL LDNFEWQDGY 490 500 510 520 KNRFGLYYVD FKNNLTRYEK ESAKYYKDFL AQGVRPSALK RDEL
	Isoform 2 [UniParc]. Checksum: 00070A61E760B27A	FASTA	522	59,493
	10 20 30 40 50 60 MALQKFPLMG LLLLLTILVS VTTAVDDPVC PATSKLSRAS FPNGFLFGTA TAAFQVEGAI 70 80 90 100 110 120 NETCRGPALW DIYCRRNPER CSGDHADVAV DFFHRYKEDI QLMKNLNTDA FRLSIAWSRI 130 140 150 160 170 180 FPHGRKEKGV SQAGVQFYHE LIDELLKNVP FVTVFHWDTP QDLEDEYGGF LSQNIVKDFR 190 200 210 220 230 240 EYADYVFTEY GGKVKNWITF NEPWVFAHAG YDLGKKAPGR CSRYVPGCED REGQSGKEAY 250 260 270 280 290 300 LVSHNLLNAH AEAVEVFRQK VKGGKIGIAH SPAWFEPHDL KDSNDAPTVS RVLDFMLGWH 310 320 330 340 350 360 LEPTTSGDYP QIMKDLLGYR LPQFTAAQKA KLKDSTDFVG LNYYTSTFSN YNEKPDPSKP 370 380 390 400 410 420 SWKQDSLVSW EPKNVDHSAI GSMPLTAALP VYAKGFRKLL KYIKDKYANP EIMIMENGYG 430 440 450 460 470 480 DKLGTTDSVD VGTADHNRKY YLQRHLLAMN EAICIDKVRV TGYFVWSLLD NFEWQDGYKN 490 500 510 520 RFGLYYVDFK NNLTRYEKES AKYYKDFLAQ GVRPSALKRD EL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A phosphate-starvation inducible beta-glucosidase gene (psr3.2) isolated from Arabidopsis thaliana is a member of a distinct subfamily of the BGA family." Malboobi M.A., Lefebvre D.D. Plant Mol. Biol. 34:57-68(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1." Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A. Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U72155 Genomic DNA. Translation: AAB64244.1. Sequence problems. AC020665 Genomic DNA. Translation: AAG52157.1. AC066691 Genomic DNA. Translation: AAG51761.1. CP002684 Genomic DNA. Translation: AEE34488.1. CP002684 Genomic DNA. Translation: AEE34489.1. AY045698 mRNA. Translation: AAK74056.1. BT002684 mRNA. Translation: AAO11600.1.
IPI	IPI00526054. IPI00531939.
PIR	G96687.
RefSeq	NP_176801.1. NM_105298.3. NP_849848.1. NM_179517.2.
UniGene	At.18035. At.75574.
3D structure databases
HSSP	HSSP built from PDB template 2CBU based on UniProtKB Q08638.
ProteinModelPortal	Q9C525.
SMR	Q9C525. Positions 34-503.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G66270.1-P.
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Proteomic databases
PaxDb	Q9C525.
PRIDE	Q9C525.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G66270.1; AT1G66270.1; AT1G66270.
GeneID	842944.
KEGG	ath:AT1G66270.
Organism-specific databases
TAIR	At1g66270.
Phylogenomic databases
eggNOG	COG2723.
HOGENOM	HOG000088630.
InParanoid	Q9C525.
KO	K01188.
OMA	ANIVEPR.
PhylomeDB	Q9C525.
ProtClustDB	CLSN2679905.
Gene expression databases
Genevestigator	Q9C525.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10353. PTHR10353. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00572. GLYCOSYL_HYDROL_F1_1. False negative. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGL21_ARATH

Accession

Primary (citable) accession number: Q9C525
Secondary accession number(s): O23656, Q3ECH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program