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Q9C525 (BGL21_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 21

Short name=AtBGLU21
EC=3.2.1.21
Alternative name(s):
Protein PHOSPHATE STARVATION-RESPONSE 3.2
Gene names
Name:BGLU21
Synonyms:PSR3.2
Ordered Locus Names:At1g66270
ORF Names:T27F4.2, T6J19.2
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Subcellular location

Endoplasmic reticulum lumen Potential.

Induction

By phosphate starvation in roots. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Sequence caution

The sequence AAB64244.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9C525-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9C525-2)

The sequence of this isoform differs from the canonical sequence as follows:
     149-150: Missing.
Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 524500Beta-glucosidase 21
PRO_0000389583

Regions

Region475 – 4762Substrate binding By similarity
Motif521 – 5244Prevents secretion from ER Potential

Sites

Active site2041Proton donor By similarity
Active site4181Nucleophile By similarity
Binding site551Substrate By similarity
Binding site1581Substrate By similarity
Binding site2031Substrate By similarity
Binding site3461Substrate By similarity
Binding site4681Substrate By similarity

Amino acid modifications

Glycosylation611N-linked (GlcNAc...) Potential
Glycosylation4941N-linked (GlcNAc...) Potential
Disulfide bond223 ↔ 230 By similarity

Natural variations

Alternative sequence149 – 1502Missing in isoform 2.
VSP_038457

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 8848C9751C15DFF9

FASTA52459,664
        10         20         30         40         50         60 
MALQKFPLMG LLLLLTILVS VTTAVDDPVC PATSKLSRAS FPNGFLFGTA TAAFQVEGAI 

        70         80         90        100        110        120 
NETCRGPALW DIYCRRNPER CSGDHADVAV DFFHRYKEDI QLMKNLNTDA FRLSIAWSRI 

       130        140        150        160        170        180 
FPHGRKEKGV SQAGVQFYHE LIDELLKNGI VPFVTVFHWD TPQDLEDEYG GFLSQNIVKD 

       190        200        210        220        230        240 
FREYADYVFT EYGGKVKNWI TFNEPWVFAH AGYDLGKKAP GRCSRYVPGC EDREGQSGKE 

       250        260        270        280        290        300 
AYLVSHNLLN AHAEAVEVFR QKVKGGKIGI AHSPAWFEPH DLKDSNDAPT VSRVLDFMLG 

       310        320        330        340        350        360 
WHLEPTTSGD YPQIMKDLLG YRLPQFTAAQ KAKLKDSTDF VGLNYYTSTF SNYNEKPDPS 

       370        380        390        400        410        420 
KPSWKQDSLV SWEPKNVDHS AIGSMPLTAA LPVYAKGFRK LLKYIKDKYA NPEIMIMENG 

       430        440        450        460        470        480 
YGDKLGTTDS VDVGTADHNR KYYLQRHLLA MNEAICIDKV RVTGYFVWSL LDNFEWQDGY 

       490        500        510        520 
KNRFGLYYVD FKNNLTRYEK ESAKYYKDFL AQGVRPSALK RDEL 

« Hide

Isoform 2 [UniParc].

Checksum: 00070A61E760B27A
Show »

FASTA52259,493

References

« Hide 'large scale' references
[1]"A phosphate-starvation inducible beta-glucosidase gene (psr3.2) isolated from Arabidopsis thaliana is a member of a distinct subfamily of the BGA family."
Malboobi M.A., Lefebvre D.D.
Plant Mol. Biol. 34:57-68(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1."
Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.
Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U72155 Genomic DNA. Translation: AAB64244.1. Sequence problems.
AC020665 Genomic DNA. Translation: AAG52157.1.
AC066691 Genomic DNA. Translation: AAG51761.1.
CP002684 Genomic DNA. Translation: AEE34488.1.
CP002684 Genomic DNA. Translation: AEE34489.1.
AY045698 mRNA. Translation: AAK74056.1.
BT002684 mRNA. Translation: AAO11600.1.
PIRG96687.
RefSeqNP_176801.1. NM_105298.3.
NP_849848.1. NM_179517.2.
UniGeneAt.18035.
At.75574.

3D structure databases

ProteinModelPortalQ9C525.
SMRQ9C525. Positions 34-503.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9C525. 1 interaction.
STRING3702.AT1G66270.1-P.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbQ9C525.
PRIDEQ9C525.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G66270.1; AT1G66270.1; AT1G66270. [Q9C525-1]
GeneID842944.
KEGGath:AT1G66270.

Organism-specific databases

TAIRAT1G66270.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088630.
InParanoidQ9C525.
KOK01188.
OMAPRREQST.
PhylomeDBQ9C525.
ProtClustDBCLSN2679905.

Enzyme and pathway databases

BioCycARA:AT1G66270-MONOMER.
ARA:GQT-1025-MONOMER.

Gene expression databases

GenevestigatorQ9C525.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00014. ER_TARGET. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGL21_ARATH
AccessionPrimary (citable) accession number: Q9C525
Secondary accession number(s): O23656, Q3ECH6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names