Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9C525

- BGL21_ARATH

UniProt

Q9C525 - BGL21_ARATH

Protein

Beta-glucosidase 21

Gene

BGLU21

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Beta-D-glucosidase active on scopolin >> esculin >> 4-MU-glucoside > DIMBOA-glucoside. No activity with pNP-glucoside, oNP-glucoside and sinigrin as substrates.1 Publication

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.1 Publication

    Enzyme regulationi

    Activated upon binding to PBP1 or PBP2.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei55 – 551SubstrateBy similarity
    Binding sitei158 – 1581SubstrateBy similarity
    Binding sitei203 – 2031SubstrateBy similarity
    Active sitei204 – 2041Proton donorBy similarity
    Binding sitei346 – 3461SubstrateBy similarity
    Active sitei418 – 4181NucleophileBy similarity
    Binding sitei468 – 4681SubstrateBy similarity

    GO - Molecular functioni

    1. beta-glucosidase activity Source: TAIR

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. cellular response to cold Source: TAIR
    3. cellular response to phosphate starvation Source: UniProtKB
    4. cellular response to salt stress Source: TAIR
    5. coumarin metabolic process Source: TAIR
    6. response to osmotic stress Source: TAIR
    7. response to salt stress Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT1G66270-MONOMER.
    ARA:GQT-1025-MONOMER.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 21 (EC:3.2.1.21)
    Short name:
    AtBGLU21
    Alternative name(s):
    Protein PHOSPHATE STARVATION-RESPONSE 3.2
    Gene namesi
    Name:BGLU21
    Synonyms:PSR3.2
    Ordered Locus Names:At1g66270
    ORF Names:T27F4.2, T6J19.2
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G66270.

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum Source: TAIR
    2. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    3. membrane Source: TAIR
    4. plasmodesma Source: TAIR
    5. vacuole Source: TAIR

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 524500Beta-glucosidase 21PRO_0000389583Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi223 ↔ 230By similarity
    Glycosylationi494 – 4941N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9C525.
    PRIDEiQ9C525.

    Expressioni

    Tissue specificityi

    Expressed exclusively in roots.1 Publication

    Inductioni

    Up-regulated by cold, 2,4-D, methyl jasmonate and phosphate starvation.2 Publications

    Gene expression databases

    GenevestigatoriQ9C525.

    Interactioni

    Subunit structurei

    Component of the PYK10 complex, at least composed of PYK10/BGLU23, BGLU21, BGLU22, JAL22, JAL23, PBP1/JAL30, PBP2/JAL31, JAL32, JAL33, JAL34, JAL35, GLL22 and GLL23.1 Publication

    Protein-protein interaction databases

    IntActiQ9C525. 1 interaction.
    STRINGi3702.AT1G66270.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9C525.
    SMRiQ9C525. Positions 34-503.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni475 – 4762Substrate bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi521 – 5244Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088630.
    InParanoidiQ9C525.
    KOiK01188.
    OMAiGYTISTR.
    PhylomeDBiQ9C525.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9C525-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MALQKFPLMG LLLLLTILVS VTTAVDDPVC PATSKLSRAS FPNGFLFGTA    50
    TAAFQVEGAI NETCRGPALW DIYCRRNPER CSGDHADVAV DFFHRYKEDI 100
    QLMKNLNTDA FRLSIAWSRI FPHGRKEKGV SQAGVQFYHE LIDELLKNGI 150
    VPFVTVFHWD TPQDLEDEYG GFLSQNIVKD FREYADYVFT EYGGKVKNWI 200
    TFNEPWVFAH AGYDLGKKAP GRCSRYVPGC EDREGQSGKE AYLVSHNLLN 250
    AHAEAVEVFR QKVKGGKIGI AHSPAWFEPH DLKDSNDAPT VSRVLDFMLG 300
    WHLEPTTSGD YPQIMKDLLG YRLPQFTAAQ KAKLKDSTDF VGLNYYTSTF 350
    SNYNEKPDPS KPSWKQDSLV SWEPKNVDHS AIGSMPLTAA LPVYAKGFRK 400
    LLKYIKDKYA NPEIMIMENG YGDKLGTTDS VDVGTADHNR KYYLQRHLLA 450
    MNEAICIDKV RVTGYFVWSL LDNFEWQDGY KNRFGLYYVD FKNNLTRYEK 500
    ESAKYYKDFL AQGVRPSALK RDEL 524
    Length:524
    Mass (Da):59,664
    Last modified:June 1, 2001 - v1
    Checksum:i8848C9751C15DFF9
    GO
    Isoform 2 (identifier: Q9C525-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         149-150: Missing.

    Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.

    Show »
    Length:522
    Mass (Da):59,493
    Checksum:i00070A61E760B27A
    GO

    Sequence cautioni

    The sequence AAB64244.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei149 – 1502Missing in isoform 2. CuratedVSP_038457

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U72155 Genomic DNA. Translation: AAB64244.1. Sequence problems.
    AC020665 Genomic DNA. Translation: AAG52157.1.
    AC066691 Genomic DNA. Translation: AAG51761.1.
    CP002684 Genomic DNA. Translation: AEE34488.1.
    CP002684 Genomic DNA. Translation: AEE34489.1.
    AY045698 mRNA. Translation: AAK74056.1.
    BT002684 mRNA. Translation: AAO11600.1.
    PIRiG96687.
    RefSeqiNP_176801.1. NM_105298.3. [Q9C525-1]
    NP_849848.1. NM_179517.2. [Q9C525-2]
    UniGeneiAt.18035.
    At.75574.

    Genome annotation databases

    EnsemblPlantsiAT1G66270.1; AT1G66270.1; AT1G66270. [Q9C525-1]
    GeneIDi842944.
    KEGGiath:AT1G66270.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U72155 Genomic DNA. Translation: AAB64244.1 . Sequence problems.
    AC020665 Genomic DNA. Translation: AAG52157.1 .
    AC066691 Genomic DNA. Translation: AAG51761.1 .
    CP002684 Genomic DNA. Translation: AEE34488.1 .
    CP002684 Genomic DNA. Translation: AEE34489.1 .
    AY045698 mRNA. Translation: AAK74056.1 .
    BT002684 mRNA. Translation: AAO11600.1 .
    PIRi G96687.
    RefSeqi NP_176801.1. NM_105298.3. [Q9C525-1 ]
    NP_849848.1. NM_179517.2. [Q9C525-2 ]
    UniGenei At.18035.
    At.75574.

    3D structure databases

    ProteinModelPortali Q9C525.
    SMRi Q9C525. Positions 34-503.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9C525. 1 interaction.
    STRINGi 3702.AT1G66270.1-P.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PaxDbi Q9C525.
    PRIDEi Q9C525.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G66270.1 ; AT1G66270.1 ; AT1G66270 . [Q9C525-1 ]
    GeneIDi 842944.
    KEGGi ath:AT1G66270.

    Organism-specific databases

    TAIRi AT1G66270.

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088630.
    InParanoidi Q9C525.
    KOi K01188.
    OMAi GYTISTR.
    PhylomeDBi Q9C525.

    Enzyme and pathway databases

    BioCyci ARA:AT1G66270-MONOMER.
    ARA:GQT-1025-MONOMER.

    Gene expression databases

    Genevestigatori Q9C525.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A phosphate-starvation inducible beta-glucosidase gene (psr3.2) isolated from Arabidopsis thaliana is a member of a distinct subfamily of the BGA family."
      Malboobi M.A., Lefebvre D.D.
      Plant Mol. Biol. 34:57-68(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. Cited for: GENE FAMILY, NOMENCLATURE.
    6. "Antagonistic jacalin-related lectins regulate the size of ER body-type beta-glucosidase complexes in Arabidopsis thaliana."
      Nagano A.J., Fukao Y., Fujiwara M., Nishimura M., Hara-Nishimura I.
      Plant Cell Physiol. 49:969-980(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE PYK10 COMPLEX.
    7. "Scopolin-hydrolyzing beta-glucosidases in roots of Arabidopsis."
      Ahn Y.O., Shimizu B., Sakata K., Gantulga D., Zhou C., Zhou Z., Bevan D.R., Esen A.
      Plant Cell Physiol. 51:132-143(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiBGL21_ARATH
    AccessioniPrimary (citable) accession number: Q9C525
    Secondary accession number(s): O23656, Q3ECH6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3