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Q9C525

- BGL21_ARATH

UniProt

Q9C525 - BGL21_ARATH

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Protein
Beta-glucosidase 21
Gene
BGLU21, PSR3.2, At1g66270, T27F4.2, T6J19.2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551Substrate By similarity
Binding sitei158 – 1581Substrate By similarity
Binding sitei203 – 2031Substrate By similarity
Active sitei204 – 2041Proton donor By similarity
Binding sitei346 – 3461Substrate By similarity
Active sitei418 – 4181Nucleophile By similarity
Binding sitei468 – 4681Substrate By similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: TAIR

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cellular response to cold Source: TAIR
  3. cellular response to phosphate starvation Source: UniProtKB
  4. cellular response to salt stress Source: TAIR
  5. coumarin metabolic process Source: TAIR
  6. response to osmotic stress Source: TAIR
  7. response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT1G66270-MONOMER.
ARA:GQT-1025-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 21 (EC:3.2.1.21)
Short name:
AtBGLU21
Alternative name(s):
Protein PHOSPHATE STARVATION-RESPONSE 3.2
Gene namesi
Name:BGLU21
Synonyms:PSR3.2
Ordered Locus Names:At1g66270
ORF Names:T27F4.2, T6J19.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G66270.

Subcellular locationi

Endoplasmic reticulum lumen Reviewed prediction

GO - Cellular componenti

  1. endoplasmic reticulum Source: TAIR
  2. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  3. membrane Source: TAIR
  4. plasmodesma Source: TAIR
  5. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed prediction
Add
BLAST
Chaini25 – 524500Beta-glucosidase 21
PRO_0000389583Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi61 – 611N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi223 ↔ 230 By similarity
Glycosylationi494 – 4941N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9C525.
PRIDEiQ9C525.

Expressioni

Inductioni

By phosphate starvation in roots.1 Publication

Gene expression databases

GenevestigatoriQ9C525.

Interactioni

Protein-protein interaction databases

IntActiQ9C525. 1 interaction.
STRINGi3702.AT1G66270.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9C525.
SMRiQ9C525. Positions 34-503.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni475 – 4762Substrate binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi521 – 5244Prevents secretion from ER Reviewed prediction

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiQ9C525.
KOiK01188.
OMAiGYTISTR.
PhylomeDBiQ9C525.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9C525-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MALQKFPLMG LLLLLTILVS VTTAVDDPVC PATSKLSRAS FPNGFLFGTA    50
TAAFQVEGAI NETCRGPALW DIYCRRNPER CSGDHADVAV DFFHRYKEDI 100
QLMKNLNTDA FRLSIAWSRI FPHGRKEKGV SQAGVQFYHE LIDELLKNGI 150
VPFVTVFHWD TPQDLEDEYG GFLSQNIVKD FREYADYVFT EYGGKVKNWI 200
TFNEPWVFAH AGYDLGKKAP GRCSRYVPGC EDREGQSGKE AYLVSHNLLN 250
AHAEAVEVFR QKVKGGKIGI AHSPAWFEPH DLKDSNDAPT VSRVLDFMLG 300
WHLEPTTSGD YPQIMKDLLG YRLPQFTAAQ KAKLKDSTDF VGLNYYTSTF 350
SNYNEKPDPS KPSWKQDSLV SWEPKNVDHS AIGSMPLTAA LPVYAKGFRK 400
LLKYIKDKYA NPEIMIMENG YGDKLGTTDS VDVGTADHNR KYYLQRHLLA 450
MNEAICIDKV RVTGYFVWSL LDNFEWQDGY KNRFGLYYVD FKNNLTRYEK 500
ESAKYYKDFL AQGVRPSALK RDEL 524
Length:524
Mass (Da):59,664
Last modified:June 1, 2001 - v1
Checksum:i8848C9751C15DFF9
GO
Isoform 2 (identifier: Q9C525-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     149-150: Missing.

Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.

Show »
Length:522
Mass (Da):59,493
Checksum:i00070A61E760B27A
GO

Sequence cautioni

The sequence AAB64244.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei149 – 1502Missing in isoform 2.
VSP_038457

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U72155 Genomic DNA. Translation: AAB64244.1. Sequence problems.
AC020665 Genomic DNA. Translation: AAG52157.1.
AC066691 Genomic DNA. Translation: AAG51761.1.
CP002684 Genomic DNA. Translation: AEE34488.1.
CP002684 Genomic DNA. Translation: AEE34489.1.
AY045698 mRNA. Translation: AAK74056.1.
BT002684 mRNA. Translation: AAO11600.1.
PIRiG96687.
RefSeqiNP_176801.1. NM_105298.3. [Q9C525-1]
NP_849848.1. NM_179517.2. [Q9C525-2]
UniGeneiAt.18035.
At.75574.

Genome annotation databases

EnsemblPlantsiAT1G66270.1; AT1G66270.1; AT1G66270. [Q9C525-1]
GeneIDi842944.
KEGGiath:AT1G66270.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U72155 Genomic DNA. Translation: AAB64244.1 . Sequence problems.
AC020665 Genomic DNA. Translation: AAG52157.1 .
AC066691 Genomic DNA. Translation: AAG51761.1 .
CP002684 Genomic DNA. Translation: AEE34488.1 .
CP002684 Genomic DNA. Translation: AEE34489.1 .
AY045698 mRNA. Translation: AAK74056.1 .
BT002684 mRNA. Translation: AAO11600.1 .
PIRi G96687.
RefSeqi NP_176801.1. NM_105298.3. [Q9C525-1 ]
NP_849848.1. NM_179517.2. [Q9C525-2 ]
UniGenei At.18035.
At.75574.

3D structure databases

ProteinModelPortali Q9C525.
SMRi Q9C525. Positions 34-503.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9C525. 1 interaction.
STRINGi 3702.AT1G66270.1-P.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbi Q9C525.
PRIDEi Q9C525.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G66270.1 ; AT1G66270.1 ; AT1G66270 . [Q9C525-1 ]
GeneIDi 842944.
KEGGi ath:AT1G66270.

Organism-specific databases

TAIRi AT1G66270.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088630.
InParanoidi Q9C525.
KOi K01188.
OMAi GYTISTR.
PhylomeDBi Q9C525.

Enzyme and pathway databases

BioCyci ARA:AT1G66270-MONOMER.
ARA:GQT-1025-MONOMER.

Gene expression databases

Genevestigatori Q9C525.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A phosphate-starvation inducible beta-glucosidase gene (psr3.2) isolated from Arabidopsis thaliana is a member of a distinct subfamily of the BGA family."
    Malboobi M.A., Lefebvre D.D.
    Plant Mol. Biol. 34:57-68(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGL21_ARATH
AccessioniPrimary (citable) accession number: Q9C525
Secondary accession number(s): O23656, Q3ECH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: June 1, 2001
Last modified: May 14, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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