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Q9C510 (PPA6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Purple acid phosphatase 6

EC=3.1.3.2
Gene names
Name:PAP6
Ordered Locus Names:At1g56360
ORF Names:F13N6.16, F14G9.2
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

Specifically expressed in flowers. Ref.1

Sequence similarities

Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandIron
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdephosphorylation

Inferred from sequence or structural similarity Ref.1. Source: GOC

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionacid phosphatase activity

Inferred from sequence or structural similarity Ref.1. Source: TAIR

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 466446Purple acid phosphatase 6
PRO_0000372811

Regions

Region351 – 3533Substrate binding By similarity

Sites

Active site3241Proton donor By similarity
Metal binding1641Iron By similarity
Metal binding1921Iron By similarity
Metal binding1921Zinc By similarity
Metal binding1951Iron By similarity
Metal binding2291Zinc By similarity
Metal binding3141Zinc By similarity
Metal binding3511Zinc By similarity
Metal binding3531Iron By similarity
Binding site2291Substrate By similarity

Amino acid modifications

Glycosylation881N-linked (GlcNAc...) Potential
Glycosylation1721N-linked (GlcNAc...) Potential
Glycosylation3671N-linked (GlcNAc...) Potential
Glycosylation4241N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict174 – 1752TL → AS in AAW29947. Ref.1
Sequence conflict3571R → G in AAW29947. Ref.1
Sequence conflict4421A → T in AAW29947. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9C510 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: C7743C328422F3FC

FASTA46652,830
        10         20         30         40         50         60 
MKNLVIFAFL FLSITTVING GITSKFVRQA LPSIEMSLDT FPSPGGYNTP EQVHLTQGDH 

        70         80         90        100        110        120 
DGRGMIVSWV TPLNLAGSNV VTYWIATNGS DVKPAKKRAH ASTKSYRFYD YSSGFLHHAT 

       130        140        150        160        170        180 
IKGLEYDTKY IYEVGTDKSV RQFSFTTPPK IGPDVPYTFG IIGDLGQTYA SNETLYHYMS 

       190        200        210        220        230        240 
NPKGQAVLFA GDLSYADDHP NHDQRKWDTW GRFMEPCAAY QPFIFAAGNH EIDFVPNIGE 

       250        260        270        280        290        300 
PHAFKPYTHR YPNAYKASQS TSPLWYSVRR ASAHIIVLSS YSAYGKYTPQ YIWLEQELKN 

       310        320        330        340        350        360 
VNREETPWLI VIVHSPWYNS NNYHYMEGES MRVMFESWLV NSKVDLVLSG HVHAYERSER 

       370        380        390        400        410        420 
ISNIKYNITN GLSSPVKDPN APIYITIGDG GNIEGIANSF VDPQPSYSAY REASFGHAVL 

       430        440        450        460 
EIMNRTHAQY TWHRNQDNEP VAADSIMLHN RHFFPVEEIV SSNIRA 

« Hide

References

« Hide 'large scale' references
[1]"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Arabidopsis ORF Clones."
Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY842022 mRNA. Translation: AAW29947.1.
AC058785 Genomic DNA. Translation: AAG51511.1.
AC069159 Genomic DNA. Translation: AAG50924.1.
CP002684 Genomic DNA. Translation: AEE33383.1.
DQ446366 mRNA. Translation: ABE65716.1.
BT026359 mRNA. Translation: ABH04466.1.
PIRF96605.
RefSeqNP_176033.1. NM_104516.1.
UniGeneAt.51194.

3D structure databases

ProteinModelPortalQ9C510.
SMRQ9C510. Positions 39-458.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ9C510.
PRIDEQ9C510.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G56360.1; AT1G56360.1; AT1G56360.
GeneID842090.
KEGGath:AT1G56360.

Organism-specific databases

TAIRAT1G56360.

Phylogenomic databases

eggNOGCOG1409.
HOGENOMHOG000238330.
InParanoidQ9C510.
OMAYWIATNG.
PhylomeDBQ9C510.
ProtClustDBCLSN2682888.

Enzyme and pathway databases

BioCycARA:AT1G56360-MONOMER.

Gene expression databases

GenevestigatorQ9C510.

Family and domain databases

Gene3D2.60.40.380. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR003961. Fibronectin_type3.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SMARTSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMSSF49363. SSF49363. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPA6_ARATH
AccessionPrimary (citable) accession number: Q9C510
Secondary accession number(s): Q5MAV2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names