Reviewed, UniProtKB/Swiss-Prot Q9C510 (PPA6_ARATH)

Names and origin

Protein names

Recommended name:
    Purple acid phosphatase 6
    EC=3.1.3.2

Gene names

Name:	PAP6
Ordered Locus Names:	At1g56360
ORF Names:	F13N6.16, F14G9.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Specifically expressed in flowers. Ref.1
Sequence similarities	Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 466	446	Purple acid phosphatase 6		PRO_0000372811
Regions
	Region	351 – 353	3	Substrate binding By similarity
Sites
	Active site	324	1	Proton donor By similarity
	Metal binding	164	1	Iron By similarity
	Metal binding	192	1	Iron By similarity
	Metal binding	192	1	Zinc By similarity
	Metal binding	195	1	Iron By similarity
	Metal binding	229	1	Zinc By similarity
	Metal binding	314	1	Zinc By similarity
	Metal binding	351	1	Zinc By similarity
	Metal binding	353	1	Iron By similarity
	Binding site	229	1	Substrate By similarity
Amino acid modifications
	Glycosylation	88	1	N-linked (GlcNAc...) Potential
	Glycosylation	172	1	N-linked (GlcNAc...) Potential
	Glycosylation	367	1	N-linked (GlcNAc...) Potential
	Glycosylation	424	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	174 – 175	2	TL → AS in AAW29947. Ref.1
	Sequence conflict	357	1	R → G in AAW29947. Ref.1
	Sequence conflict	442	1	A → T in AAW29947. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9C510-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: C7743C328422F3FC	FASTA	466	52,830
	10 20 30 40 50 60 MKNLVIFAFL FLSITTVING GITSKFVRQA LPSIEMSLDT FPSPGGYNTP EQVHLTQGDH 70 80 90 100 110 120 DGRGMIVSWV TPLNLAGSNV VTYWIATNGS DVKPAKKRAH ASTKSYRFYD YSSGFLHHAT 130 140 150 160 170 180 IKGLEYDTKY IYEVGTDKSV RQFSFTTPPK IGPDVPYTFG IIGDLGQTYA SNETLYHYMS 190 200 210 220 230 240 NPKGQAVLFA GDLSYADDHP NHDQRKWDTW GRFMEPCAAY QPFIFAAGNH EIDFVPNIGE 250 260 270 280 290 300 PHAFKPYTHR YPNAYKASQS TSPLWYSVRR ASAHIIVLSS YSAYGKYTPQ YIWLEQELKN 310 320 330 340 350 360 VNREETPWLI VIVHSPWYNS NNYHYMEGES MRVMFESWLV NSKVDLVLSG HVHAYERSER 370 380 390 400 410 420 ISNIKYNITN GLSSPVKDPN APIYITIGDG GNIEGIANSF VDPQPSYSAY REASFGHAVL 430 440 450 460 EIMNRTHAQY TWHRNQDNEP VAADSIMLHN RHFFPVEEIV SSNIRA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower." Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D. Plant Mol. Biol. 59:581-594(2005) [PubMed: 16244908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF Clones." Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation." Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D. J. Biol. Chem. 277:27772-27781(2002) [PubMed: 12021284] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY842022 mRNA. Translation: AAW29947.1. AC058785 Genomic DNA. Translation: AAG51511.1. AC069159 Genomic DNA. Translation: AAG50924.1. DQ446366 mRNA. Translation: ABE65716.1. BT026359 mRNA. Translation: ABH04466.1.
IPI	IPI00519222.
PIR	F96605.
RefSeq	NP_176033.1.
UniGene	At.51194
3D structure databases
HSSP	HSSP built from PDB template 4KBP based on UniProtKB P80366.
ModBase	Search...
Proteomic databases
PRIDE	Q9C510.
Genome annotation databases
GeneID	842090.
GenomeReviews	Gene locus AT1G56360 in contig CT485782_GR.
KEGG	ath:AT1G56360.
NMPDR	fig|3702.1.peg.5187.
Organism-specific databases
TAIR	At1g56360.
Phylogenomic databases
OMA	PLWYSVR.
Gene expression databases
Genevestigator	Q9C510.
Family and domain databases
InterPro	IPR003961. FN_III. IPR004843. M-pesterase. IPR015914. Purple_acid_Pase_N. [Graphical view]
Gene3D	G3DSA:2.60.40.380. Purple_acid_Pase_N. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
SMART	SM00060. FN3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPA6_ARATH

Accession

Primary (citable) accession number: Q9C510
Secondary accession number(s): Q5MAV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	June 1, 2001
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)