Q9C509 (SGPL_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sphingosine-1-phosphate lyase
Short name=S1PL
Short name=SP-lyase
Short name=SPL
EC=4.1.2.27

Alternative name(s):
Sphingosine-1-phosphate aldolase

Gene names

Ordered Locus Names:	At1g27980
ORF Names:	F13K9.8

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis By similarity.
Catalytic activity	Sphinganine 1-phosphate = phosphoethanolamine + palmitaldehyde.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Lipid metabolism; sphingolipid metabolism.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity.
Sequence similarities	Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.
Sequence caution	The sequence AAM62669.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Apoptosis Lipid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW carboxylic acid metabolic process Inferred from electronic annotation. Source: InterPro sphingolipid catabolic process Inferred from mutant phenotype. Source: TAIR
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	carboxy-lyase activity Inferred from electronic annotation. Source: InterPro pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro sphinganine-1-phosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 544	544	Sphingosine-1-phosphate lyase		PRO_0000147017
Regions
	Topological domain	1 – 29	29	Lumenal Potential
	Transmembrane	30 – 50	21	Helical; Signal-anchor for type III membrane protein; Potential
	Topological domain	51 – 544	494	Cytoplasmic Potential
Amino acid modifications
	Modified residue	349	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9C509 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: CC5C9952C2317248	FASTA	544	59,478
	10 20 30 40 50 60 MDSFSYSSMK SMLIQARGSL NSRLSEFEPL VLLLVPLVSL FLAQIIGSVF GVVHEKGLKA 70 80 90 100 110 120 CLIGFIMGLL KMIPGVQNYI DAEKQKVVDQ LQSGSSSKKK NKTEVLPVKG LGVEVLEKME 130 140 150 160 170 180 NEKRNDAIWQ GKCSGTVYIG GAESEGHFSL INQACSMFAH TNPLHIDVFQ SVVRFESEVV 190 200 210 220 230 240 AMTAALLGSK ETASGGQICG NMTSGGTESI VLAVKSSRDY MKYKKGITRP EMIIPESGHS 250 260 270 280 290 300 AYDKAAQYFK IKLWRVPVDK DFRADVKATR RHINRNTIMI VGSAPGFPHG IIDPIEELGQ 310 320 330 340 350 360 LALSYGICFH VDLCLGGFVL PFARKLGYQI PPFDFSVQGV TSISVDVHKY GLAPKGTSTV 370 380 390 400 410 420 LYRNHEIRKH QFVAVTEWSG GLYVSPTIAG SRPGSLVAGA WAAMMSLGEE GYLQNTSKIM 430 440 450 460 470 480 EASKRLEEGV REIHELFVIG KPDMTIVAFG SKALDIFEVN DIMSSKGWHL NALQRPNSIH 490 500 510 520 530 540 ICITLQHVPV VDDFLRDLRE AVETVKANPG PITGGLAPIY GAAGKMPDRG MVNELLVSFM DSQY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 412-544.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC069471 Genomic DNA. Translation: AAG51494.1. CP002684 Genomic DNA. Translation: AEE30898.1. AF360166 mRNA. Translation: AAK25876.1. AY113914 mRNA. Translation: AAM44962.1. AY085442 mRNA. Translation: AAM62669.1. Different initiation.
IPI	IPI00542236.
PIR	C86405.
RefSeq	NP_174119.1. NM_102563.3.
UniGene	At.17690. At.66881.
3D structure databases
ProteinModelPortal	Q9C509.
SMR	Q9C509. Positions 99-544.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9C509.
Proteomic databases
PRIDE	Q9C509.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G27980.1; AT1G27980.1; AT1G27980.
GeneID	839691.
GenomeReviews	Gene locus AT1G27980 in contig CT485782_GR.
KEGG	ath:AT1G27980.
NMPDR	fig|3702.1.peg.3131.
Organism-specific databases
TAIR	At1g27980.
Phylogenomic databases
GeneTree	EPGT00070000030479.
HOGENOM	HBG657947.
InParanoid	Q9C509.
OMA	IIAACWA.
PhylomeDB	Q9C509.
ProtClustDB	CLSN2679351.
Enzyme and pathway databases
BioCyc	ARA:AT1G27980-MONOMER. MetaCyc:AT1G27980-MONOMER.
BRENDA	4.1.2.27. 399.
Gene expression databases
ArrayExpress	Q9C509.
Genevestigator	Q9C509.
GermOnline	AT1G27980. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002129. PyrdxlP-dep_de-COase. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
KO	K01634.
PANTHER	PTHR11999. Pyridoxal_deC. 1 hit.
Pfam	PF00282. Pyridoxal_deC. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00392. DDC_GAD_HDC_YDC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SGPL_ARATH

Accession

Primary (citable) accession number: Q9C509
Secondary accession number(s): Q8LEF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
Last sequence update:	June 1, 2001
Last modified:	November 16, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program