Q9C500 (WPP2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WPP domain-containing protein 2

Alternative name(s):
MFP1 attachment factor 2

Gene names

Name:	WPP2
Synonyms:	MAF2
Ordered Locus Names:	At1g47200
ORF Names:	F2G19.18, F8G22.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates the mitotic activity in roots. Plays a role with HSP70-1 in facilitating WIT1 nuclear envelope targeting. Ref.5 Ref.9
Subunit structure	Binds to FPP proteins By similarity. Interacts with WAP, WIP1, WIP2 and WIP3 through its WPP domain. Interacts with WIT1 and HSP70-1. Ref.6 Ref.7 Ref.8 Ref.9
Subcellular location	Nucleus envelope. Cytoplasm. Nucleus. Golgi apparatus By similarity. Note: Associated to the nuclear envelope (NE) in undifferentiated cells of the root tip. Associated with the outer NE and the nuclear pores in interphase cells and with the immature cell plate during cytokinesis. In differentiated cells, localized in both cytoplasm and nucleus. Accumulate in speckles of the cytoplasm belonging to the Golgi apparatus By similarity. Appears at the NE as cells reenter the cell cycle during dedifferentiation. Ref.5
Tissue specificity	Expressed in roots, stems, leaves and flowers. Ref.5
Domain	The WPP domain is required for the nuclear envelope localization By similarity.

Ontologies

Keywords
Cellular component	Cytoplasm Golgi apparatus Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lateral root development Inferred from mutant phenotype Ref.5. Source: TAIR mitosis Inferred from mutant phenotype Ref.5. Source: TAIR
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from direct assay PubMed 21166475. Source: TAIR nuclear outer membrane Inferred from direct assay Ref.5. Source: TAIR plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 180	180	WPP domain-containing protein 2		PRO_0000347192
Regions
	Region	44 – 147	104	WPP
Amino acid modifications
	Modified residue	173	1	Phosphoserine Ref.10
Experimental info
	Sequence conflict	172	1	A → G in AAM62902. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9C500 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: F71F090976FA3826	FASTA	180	19,134
	10 20 30 40 50 60 MAETAETINT TISSPPPESE SSTTISAMTD PTSQEAASKD TDLTKEAESE KKPGGISLRI 70 80 90 100 110 120 WPPTQKTRDA VLNRLIETLS TESILSKRYG TLKSDDATTV AKLIEEEAYG VASNAVSSDD 130 140 150 160 170 180 DGIKILELYS KEISKRMLES VKARSNASVG NGSVEDANTD ASEVSKDDAG PASEEEKSEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Arabidopsis WPP-domain proteins are developmentally associated with the nuclear envelope and promote cell division." Patel S., Rose A., Meulia T., Dixit R., Cyr R.J., Meier I. Plant Cell 16:3260-3273(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[6]	"The plant nuclear envelope protein MAF1 has an additional location at the Golgi and binds to a novel Golgi-associated coiled-coil protein." Patel S., Brkljacic J., Gindullis F., Rose A., Meier I. Planta 222:1028-1040(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH WAP.
[7]	"Anchorage of plant RanGAP to the nuclear envelope involves novel nuclear-pore-associated proteins." Xu X.M., Meulia T., Meier I. Curr. Biol. 17:1157-1163(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH WIP1; WIP2 AND WIP3.
[8]	"Two distinct interacting classes of nuclear envelope-associated coiled-coil proteins are required for the tissue-specific nuclear envelope targeting of Arabidopsis RanGAP." Zhao Q., Brkljacic J., Meier I. Plant Cell 20:1639-1651(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH WIT1.
[9]	"WPP-domain proteins mimic the activity of the HSC70-1 chaperone in preventing mistargeting of RanGAP1-anchoring protein WIT1." Brkljacic J., Zhao Q., Meier I. Plant Physiol. 151:142-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH WIT1 AND HSP70-1, FUNCTION.
[10]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-173, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC079677 Genomic DNA. Translation: AAG52640.1. AC083835 Genomic DNA. Translation: AAG50616.1. CP002684 Genomic DNA. Translation: AEE32136.1. AY072148 mRNA. Translation: AAL59970.1. AY096476 mRNA. Translation: AAM20116.1. AY085683 mRNA. Translation: AAM62902.1.
IPI	IPI00529353.
PIR	F96512.
RefSeq	NP_564498.1. NM_103613.2.
UniGene	At.17221.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9C500. 6 interactions.
Proteomic databases
PaxDb	Q9C500.
PRIDE	Q9C500.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G47200.1; AT1G47200.1; AT1G47200.
GeneID	841123.
KEGG	ath:AT1G47200.
Organism-specific databases
TAIR	At1g47200.
Phylogenomic databases
eggNOG	NOG262975.
HOGENOM	HOG000239842.
InParanoid	Q9C500.
OMA	EVSKDDA.
PhylomeDB	Q9C500.
ProtClustDB	CLSN2686648.
Gene expression databases
Genevestigator	Q9C500.
Family and domain databases
InterPro	IPR025265. WPP_dom. [Graphical view]
Pfam	PF13943. WPP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WPP2_ARATH

Accession

Primary (citable) accession number: Q9C500
Secondary accession number(s): Q8LE13

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program