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Q9C4Z5 (AQPM_METTM) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Aquaporin AqpM
Gene names
Name:aqpM
Ordered Locus Names:MTBMA_c05510
OrganismMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP]
Taxonomic identifier79929 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Channel that permits osmotically driven movement of water in both directions. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity. Exhibits also a transient but reproducible increase in the initial glycerol flux.

Subunit structure

Homotetramer.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Domain

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similarities

Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
   DomainRepeat
Transmembrane
Transmembrane helix
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processtransmembrane transport

Inferred from electronic annotation. Source: InterPro

water transport

Inferred from electronic annotation. Source: InterPro

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiontransporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 246246Aquaporin AqpM
PRO_0000064006

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 3221Helical; Potential
Topological domain33 – 5523Extracellular Potential
Transmembrane56 – 7621Helical; Potential
Topological domain77 – 10327Cytoplasmic Potential
Transmembrane104 – 12421Helical; Potential
Topological domain125 – 14521Extracellular Potential
Transmembrane146 – 16621Helical; Potential
Topological domain167 – 1726Cytoplasmic Potential
Transmembrane173 – 19321Helical; Potential
Topological domain194 – 21724Extracellular Potential
Transmembrane218 – 23821Helical; Potential
Topological domain239 – 2468Cytoplasmic Potential
Motif82 – 843NPA 1
Motif199 – 2013NPA 2

Experimental info

Sequence conflict2171N → D no nucleotide entry Ref.2
Sequence conflict2231P → S no nucleotide entry Ref.2

Secondary structure

1 .......................... 246
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9C4Z5 [UniParc].

Last modified November 30, 2010. Version 4.
Checksum: B6A31126A66257D6

FASTA24625,357
        10         20         30         40         50         60 
MVSLTKRCIA EFIGTFILVF FGAGSAAVTL MIASGGTSPN PFNIGIGLLG GLGDWVAIGL 

        70         80         90        100        110        120 
AFGFAIAASI YALGNISGCH INPAVTIGLW SVKKFPGREV VPYIIAQLLG AAFGSFIFLQ 

       130        140        150        160        170        180 
CAGIGAATVG GLGATAPFPG ISYWQAMLAE VVGTFLLMIT IMGIAVDERA PKGFAGIIIG 

       190        200        210        220        230        240 
LTVAGIITTL GNISGSSLNP ARTFGPYLND MIFAGTNLWN YYPIYVIGPI VGAVLAALTY 


QYLTSE

« Hide

References

« Hide 'large scale' references
[1]"Rapid amplification of a water channel-like gene and its flanking sequences from the Methanothermobacter marburgensis genome using a single primer PCR strategy."
Ding X., Kitagawa Y.
J. Biosci. Bioeng. 92:488-491(2001) [PubMed: 16233136] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[2]Kozono D., Ding X., Iwasaki I., Meng X., Kamagata Y., Agre P., Kitagawa Y.
Submitted (JUL-2004) to UniProtKB
Cited for: SEQUENCE REVISION TO 51; 217 AND 223.
[3]"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
J. Bacteriol. 192:5850-5851(2010) [PubMed: 20802048] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[4]"Functional expression and characterization of an archaeal aquaporin. AqpM from Methanothermobacter marburgensis."
Kozono D., Ding X., Iwasaki I., Meng X., Kamagata Y., Agre P., Kitagawa Y.
J. Biol. Chem. 278:10649-10656(2003) [PubMed: 12519768] [Abstract]
Cited for: CHARACTERIZATION.
Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB055880 Genomic DNA. Translation: BAB32660.1.
CP001710 Genomic DNA. Translation: ADL58146.1.
RefSeqYP_003849459.1. NC_014408.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2EVUX-ray2.30A1-246[»]
2F2BX-ray1.68A1-246[»]
ModBaseSearch...

Protein family/group databases

TCDB1.A.8.13.2. major intrinsic protein (MIP) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID9704259.
GenomeReviewsGene locus MTBMA_c05510 in contig CP001710_GR.
KEGGmmg:MTBMA_c05510.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAYFPIYVI.

Family and domain databases

InterProIPR012269. Aquaporin.
IPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
Gene3DG3DSA:1.20.1080.10. MIP. 1 hit.
KOK02440.
PANTHERPTHR19139. MIP. 1 hit.
PfamPF00230. MIP. 1 hit.
[Graphical view]
PRINTSPR00783. MINTRINSICP.
SUPFAMSSF81338. MIP. 1 hit.
TIGRFAMsTIGR00861. MIP. 1 hit.
PROSITEPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAQPM_METTM
AccessionPrimary (citable) accession number: Q9C4Z5
Secondary accession number(s): D9PV98
Entry history
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: November 30, 2010
Last modified: November 16, 2011
This is version 69 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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