Reviewed, UniProtKB/Swiss-Prot Q9C4Z5 (AQPM_METTM)

Names and origin

Protein names

Recommended name:
    Aquaporin aqpM

Gene names

Name:

aqpM

Organism

Methanobacterium thermoautotrophicum (strain Marburg / DSM 2133)

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Channel that permits osmotically driven movement of water in both directions. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity. Exhibits also a transient but reproducible increase in the initial glycerol flux.
Subunit structure	Homotetramer.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane
Technical term	3D-structure
Gene Ontology (GO)
Biological process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 246

246

Aquaporin aqpM

PRO_0000064006

Regions

Topological domain

1 – 11

11

Cytoplasmic Potential

Transmembrane

12 – 32

21

Potential

Topological domain

33 – 55

23

Extracellular Potential

Transmembrane

56 – 76

21

Potential

Topological domain

77 – 103

27

Cytoplasmic Potential

Transmembrane

104 – 124

21

Potential

Topological domain

125 – 145

21

Extracellular Potential

Transmembrane

146 – 166

21

Potential

Topological domain

167 – 172

6

Cytoplasmic Potential

Transmembrane

173 – 193

21

Potential

Topological domain

194 – 217

24

Extracellular Potential

Transmembrane

218 – 238

21

Potential

Topological domain

239 – 246

8

Cytoplasmic Potential

Motif

82 – 84

3

NPA 1

Motif

199 – 201

3

NPA 2

Secondary structure

1

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.

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.

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246

Helix Strand Turn

Details...

Helix

4 – 33

30

Beta strand

45 – 47

3

Turn

48 – 51

4

Helix

52 – 77

26

Helix

83 – 90

8

Turn

91 – 93

3

Helix

97 – 122

26

Helix

125 – 128

4

Helix

131 – 133

3

Helix

143 – 164

22

Helix

175 – 194

20

Helix

200 – 212

13

Beta strand

213 – 215

3

Turn

219 – 223

5

Helix

224 – 243

20

Sequences

	Sequence		Length	Mass (Da)
	Q9C4Z5-1 [UniParc]. Last modified October 11, 2004. Version 3. Checksum: E6901126A668F77E	FASTA	246	25,348
	10 20 30 40 50 60 MVSLTKRCIA EFIGTFILVF FGAGSAAVTL MIASGGTSPN PFNIGIGLLG GLGDWVAIGL 70 80 90 100 110 120 AFGFAIAASI YALGNISGCH INPAVTIGLW SVKKFPGREV VPYIIAQLLG AAFGSFIFLQ 130 140 150 160 170 180 CAGIGAATVG GLGATAPFPG ISYWQAMLAE VVGTFLLMIT IMGIAVDERA PKGFAGIIIG 190 200 210 220 230 240 LTVAGIITTL GNISGSSLNP ARTFGPYLND MIFAGTDLWN YYSIYVIGPI VGAVLAALTY QYLTSE

References

[1]	"Rapid amplification of a water channel-like gene and its flanking sequences from the Methanothermobacter marburgensis genome using a single primer PCR strategy." Ding X., Kitagawa Y. J. Biosci. Bioeng. 92:488-491(2001) [PubMed: 16233136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Kozono D., Ding X., Iwasaki I., Meng X., Kamagata Y., Agre P., Kitagawa Y. Submitted (JUL-2004) to UniProtKB Cited for: SEQUENCE REVISION TO 51; 217 AND 223.
[3]	"Functional expression and characterization of an archaeal aquaporin. AqpM from Methanothermobacter marburgensis." Kozono D., Ding X., Iwasaki I., Meng X., Kamagata Y., Agre P., Kitagawa Y. J. Biol. Chem. 278:10649-10656(2003) [PubMed: 12519768] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB055880 Genomic DNA. Translation: BAB32660.1. Sequence problems.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EVU	X-ray	2.30	A	1-246	[»]
2F2B	X-ray	1.68	A	1-246	[»]

ModBase

Search...

Protein family/group databases

TCDB

1.A.8.13.2. major intrinsic protein (MIP) family.

Family and domain databases

InterPro

IPR012269. Aquaporin.
IPR000425. MIP.
[Graphical view]

Gene3D

G3DSA:1.20.1080.10. MIP. 1 hit.

PANTHER

PTHR19139. MIP. 1 hit.

Pfam

PF00230. MIP. 1 hit.
[Graphical view]

PRINTS

PR00783. MINTRINSICP.

TIGRFAMs

TIGR00861. MIP. 1 hit.

PROSITE

PS00221. MIP. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AQPM_METTM

Accession

Primary (citable) accession number: Q9C4Z5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 3, 2003
Last sequence update:	October 11, 2004
Last modified:	February 9, 2010
This is version 54 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)