Aquaporin AqpM - Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg)

Contribute

Send feedback

Read comments (?) or add your own

Q9C4Z5 (AQPM_METTM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 78. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Aquaporin AqpM

Gene names

Name:	aqpM
Ordered Locus Names:	MTBMA_c05510

Organism

Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP]

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter ›

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Channel that permits osmotically driven movement of water in both directions. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity. Exhibits also a transient but reproducible increase in the initial glycerol flux.
Subunit structure	Homotetramer.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 246

246

Aquaporin AqpM

PRO_0000064006

Regions

Topological domain

1 – 11

Cytoplasmic Potential

Transmembrane

12 – 32

Helical; Potential

Topological domain

33 – 55

Extracellular Potential

Transmembrane

56 – 76

Helical; Potential

Topological domain

77 – 103

Cytoplasmic Potential

Transmembrane

104 – 124

Helical; Potential

Topological domain

125 – 145

Extracellular Potential

Transmembrane

146 – 166

Helical; Potential

Topological domain

167 – 172

Cytoplasmic Potential

Transmembrane

173 – 193

Helical; Potential

Topological domain

194 – 217

Extracellular Potential

Transmembrane

218 – 238

Helical; Potential

Topological domain

239 – 246

Cytoplasmic Potential

Motif

82 – 84

NPA 1

Motif

199 – 201

NPA 2

Experimental info

Sequence conflict

217

N → D no nucleotide entry Ref.2

Sequence conflict

223

P → S no nucleotide entry Ref.2

Secondary structure

246

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q9C4Z5 [UniParc].

Last modified November 30, 2010. Version 4.
Checksum: B6A31126A66257D6
Show »

FASTA

246

25,357

        10         20         30         40         50         60 
MVSLTKRCIA EFIGTFILVF FGAGSAAVTL MIASGGTSPN PFNIGIGLLG GLGDWVAIGL 

        70         80         90        100        110        120 
AFGFAIAASI YALGNISGCH INPAVTIGLW SVKKFPGREV VPYIIAQLLG AAFGSFIFLQ 

       130        140        150        160        170        180 
CAGIGAATVG GLGATAPFPG ISYWQAMLAE VVGTFLLMIT IMGIAVDERA PKGFAGIIIG 

       190        200        210        220        230        240 
LTVAGIITTL GNISGSSLNP ARTFGPYLND MIFAGTNLWN YYPIYVIGPI VGAVLAALTY 


QYLTSE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rapid amplification of a water channel-like gene and its flanking sequences from the Methanothermobacter marburgensis genome using a single primer PCR strategy." Ding X., Kitagawa Y. J. Biosci. Bioeng. 92:488-491(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[2]	Kozono D., Ding X., Iwasaki I., Meng X., Kamagata Y., Agre P., Kitagawa Y. Submitted (JUL-2004) to UniProtKB Cited for: SEQUENCE REVISION TO 51; 217 AND 223.
[3]	"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism." Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K. J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[4]	"Functional expression and characterization of an archaeal aquaporin. AqpM from Methanothermobacter marburgensis." Kozono D., Ding X., Iwasaki I., Meng X., Kamagata Y., Agre P., Kitagawa Y. J. Biol. Chem. 278:10649-10656(2003) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB055880 Genomic DNA. Translation: BAB32660.1.
CP001710 Genomic DNA. Translation: ADL58146.1.

RefSeq

YP_003849459.1. NC_014408.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EVU	X-ray	2.30	A	1-246	[»]
2F2B	X-ray	1.68	A	1-246	[»]

ModBase

Search...

Protein family/group databases

TCDB

1.A.8.13.2. major intrinsic protein (MIP) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

ADL58146; ADL58146; MTBMA_c05510.

GeneID

9704259.

KEGG

mmg:MTBMA_c05510.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HOG000288286.

K02440.

OMA

EVVITSM.

Enzyme and pathway databases

BioCyc

MMAR79929:GH5J-558-MONOMER.

Family and domain databases

Gene3D

1.20.1080.10. 1 hit.

InterPro

IPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]

PANTHER

PTHR19139. PTHR19139. 1 hit.

Pfam

PF00230. MIP. 1 hit.
[Graphical view]

PRINTS

PR00783. MINTRINSICP.

SUPFAM

SSF81338. MIP. 1 hit.

TIGRFAMs

TIGR00861. MIP. 1 hit.

PROSITE

PS00221. MIP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9C4Z5.

Entry information

Entry name

AQPM_METTM

Accession

Primary (citable) accession number: Q9C4Z5
Secondary accession number(s): D9PV98

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 3, 2003
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 78 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents