SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9C4Z5

- AQPM_METTM

UniProt

Q9C4Z5 - AQPM_METTM

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Aquaporin AqpM
Gene
aqpM, MTBMA_c05510
Organism
Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Channel that permits osmotically driven movement of water in both directions. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity. Exhibits also a transient but reproducible increase in the initial glycerol flux.

GO - Molecular functioni

  1. transporter activity Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    BioCyciMMAR79929:GH5J-554-MONOMER.

    Protein family/group databases

    TCDBi1.A.8.13.2. the major intrinsic protein (mip) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aquaporin AqpM
    Gene namesi
    Name:aqpM
    Ordered Locus Names:MTBMA_c05510
    OrganismiMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
    Taxonomic identifieri79929 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000000345: Chromosome

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111Cytoplasmic Reviewed prediction
    Add
    BLAST
    Transmembranei12 – 3221Helical; Reviewed prediction
    Add
    BLAST
    Topological domaini33 – 5523Extracellular Reviewed prediction
    Add
    BLAST
    Transmembranei56 – 7621Helical; Reviewed prediction
    Add
    BLAST
    Topological domaini77 – 10327Cytoplasmic Reviewed prediction
    Add
    BLAST
    Transmembranei104 – 12421Helical; Reviewed prediction
    Add
    BLAST
    Topological domaini125 – 14521Extracellular Reviewed prediction
    Add
    BLAST
    Transmembranei146 – 16621Helical; Reviewed prediction
    Add
    BLAST
    Topological domaini167 – 1726Cytoplasmic Reviewed prediction
    Transmembranei173 – 19321Helical; Reviewed prediction
    Add
    BLAST
    Topological domaini194 – 21724Extracellular Reviewed prediction
    Add
    BLAST
    Transmembranei218 – 23821Helical; Reviewed prediction
    Add
    BLAST
    Topological domaini239 – 2468Cytoplasmic Reviewed prediction

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 246246Aquaporin AqpM
    PRO_0000064006Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 3330
    Turni46 – 516
    Helixi52 – 7322
    Turni74 – 763
    Helixi83 – 919
    Helixi97 – 993
    Helixi100 – 12223
    Helixi125 – 1284
    Helixi131 – 1333
    Helixi143 – 16422
    Helixi175 – 19420
    Helixi200 – 21415
    Helixi219 – 2235
    Helixi224 – 24320

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2EVUX-ray2.30A1-246[»]
    2F2BX-ray1.68A1-246[»]
    ProteinModelPortaliQ9C4Z5.

    Miscellaneous databases

    EvolutionaryTraceiQ9C4Z5.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi82 – 843NPA 1
    Motifi199 – 2013NPA 2

    Domaini

    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOGENOMiHOG000288286.
    KOiK02440.
    OMAiIYVIGPI.

    Family and domain databases

    Gene3Di1.20.1080.10. 1 hit.
    InterProiIPR023271. Aquaporin-like.
    IPR000425. MIP.
    IPR022357. MIP_CS.
    [Graphical view]
    PANTHERiPTHR19139. PTHR19139. 1 hit.
    PfamiPF00230. MIP. 1 hit.
    [Graphical view]
    PRINTSiPR00783. MINTRINSICP.
    SUPFAMiSSF81338. SSF81338. 1 hit.
    TIGRFAMsiTIGR00861. MIP. 1 hit.
    PROSITEiPS00221. MIP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9C4Z5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVSLTKRCIA EFIGTFILVF FGAGSAAVTL MIASGGTSPN PFNIGIGLLG    50
    GLGDWVAIGL AFGFAIAASI YALGNISGCH INPAVTIGLW SVKKFPGREV 100
    VPYIIAQLLG AAFGSFIFLQ CAGIGAATVG GLGATAPFPG ISYWQAMLAE 150
    VVGTFLLMIT IMGIAVDERA PKGFAGIIIG LTVAGIITTL GNISGSSLNP 200
    ARTFGPYLND MIFAGTNLWN YYPIYVIGPI VGAVLAALTY QYLTSE 246
    Length:246
    Mass (Da):25,357
    Last modified:November 30, 2010 - v4
    Checksum:iB6A31126A66257D6
    GO

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti217 – 2171N → D no nucleotide entry 1 Publication
    Sequence conflicti223 – 2231P → S no nucleotide entry 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB055880 Genomic DNA. Translation: BAB32660.1.
    CP001710 Genomic DNA. Translation: ADL58146.1.
    RefSeqiYP_003849459.1. NC_014408.1.

    Genome annotation databases

    EnsemblBacteriaiADL58146; ADL58146; MTBMA_c05510.
    GeneIDi9704259.
    KEGGimmg:MTBMA_c05510.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB055880 Genomic DNA. Translation: BAB32660.1 .
    CP001710 Genomic DNA. Translation: ADL58146.1 .
    RefSeqi YP_003849459.1. NC_014408.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2EVU X-ray 2.30 A 1-246 [» ]
    2F2B X-ray 1.68 A 1-246 [» ]
    ProteinModelPortali Q9C4Z5.
    ModBasei Search...

    Protein family/group databases

    TCDBi 1.A.8.13.2. the major intrinsic protein (mip) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADL58146 ; ADL58146 ; MTBMA_c05510 .
    GeneIDi 9704259.
    KEGGi mmg:MTBMA_c05510.

    Phylogenomic databases

    HOGENOMi HOG000288286.
    KOi K02440.
    OMAi IYVIGPI.

    Enzyme and pathway databases

    BioCyci MMAR79929:GH5J-554-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q9C4Z5.

    Family and domain databases

    Gene3Di 1.20.1080.10. 1 hit.
    InterProi IPR023271. Aquaporin-like.
    IPR000425. MIP.
    IPR022357. MIP_CS.
    [Graphical view ]
    PANTHERi PTHR19139. PTHR19139. 1 hit.
    Pfami PF00230. MIP. 1 hit.
    [Graphical view ]
    PRINTSi PR00783. MINTRINSICP.
    SUPFAMi SSF81338. SSF81338. 1 hit.
    TIGRFAMsi TIGR00861. MIP. 1 hit.
    PROSITEi PS00221. MIP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Rapid amplification of a water channel-like gene and its flanking sequences from the Methanothermobacter marburgensis genome using a single primer PCR strategy."
      Ding X., Kitagawa Y.
      J. Biosci. Bioeng. 92:488-491(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
    2. Kozono D., Ding X., Iwasaki I., Meng X., Kamagata Y., Agre P., Kitagawa Y.
      Submitted (JUL-2004) to UniProtKB
      Cited for: SEQUENCE REVISION TO 51; 217 AND 223.
    3. "Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
      Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
      J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
    4. "Functional expression and characterization of an archaeal aquaporin. AqpM from Methanothermobacter marburgensis."
      Kozono D., Ding X., Iwasaki I., Meng X., Kamagata Y., Agre P., Kitagawa Y.
      J. Biol. Chem. 278:10649-10656(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

    Entry informationi

    Entry nameiAQPM_METTM
    AccessioniPrimary (citable) accession number: Q9C4Z5
    Secondary accession number(s): D9PV98
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 3, 2003
    Last sequence update: November 30, 2010
    Last modified: July 9, 2014
    This is version 84 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi