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Protein

Fructose-bisphosphate aldolase

Gene

FBA1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (RAG2)
  3. ATP-dependent 6-phosphofructokinase subunit alpha (PFK1), ATP-dependent 6-phosphofructokinase subunit beta (PFK2)
  4. Fructose-bisphosphate aldolase (FBA1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63Glyceraldehyde 3-phosphateBy similarity1
Active sitei110Proton donorBy similarity1
Metal bindingi111Zinc 1; catalyticBy similarity1
Metal bindingi145Zinc 2By similarity1
Metal bindingi175Zinc 2By similarity1
Metal bindingi227Zinc 1; catalyticBy similarity1
Binding sitei228Dihydroxyacetone phosphate; via amide nitrogenBy similarity1
Metal bindingi266Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:FBA1
Ordered Locus Names:KLLA0E07546g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome E

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001787581 – 361Fructose-bisphosphate aldolaseAdd BLAST361

Proteomic databases

PRIDEiQ9C2U0

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi284590.XP_454290.1

Structurei

3D structure databases

ProteinModelPortaliQ9C2U0
SMRiQ9C2U0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni267 – 269Dihydroxyacetone phosphate bindingBy similarity3
Regioni288 – 291Dihydroxyacetone phosphate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4153 Eukaryota
COG0191 LUCA
HOGENOMiHOG000227794
InParanoidiQ9C2U0
KOiK01624
OMAiELCKDCI
OrthoDBiEOG092C385G

Family and domain databases

CDDicd00946 FBP_aldolase_IIA, 1 hit
Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR000771 FBA_II
IPR006411 Fruct_bisP_bact
PANTHERiPTHR30559 PTHR30559, 1 hit
PfamiView protein in Pfam
PF01116 F_bP_aldolase, 1 hit
PIRSFiPIRSF001359 F_bP_aldolase_II, 1 hit
TIGRFAMsiTIGR00167 cbbA, 1 hit
TIGR01520 FruBisAldo_II_A, 1 hit
PROSITEiView protein in PROSITE
PS00602 ALDOLASE_CLASS_II_1, 1 hit
PS00806 ALDOLASE_CLASS_II_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9C2U0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAQDVLTRK TGVIVGDDVK ALFDYAKEHK FAIPAINVTS SSTVVAALEA
60 70 80 90 100
ARDNKSPIIL QTSNGGAAYF AGKGVSNEGQ NASIRGSIAA AHYIRSIAPA
110 120 130 140 150
YGIPVVLHTD HCAKKLLPWF DGMLKADEEY FAKHGEPLFS SHMLDLSEET
160 170 180 190 200
DEENIGLCVK YFTRMAKIHQ WLEMEIGITG GEEDGVNNEG TSNDKLYTTP
210 220 230 240 250
ETVFSVHEAL SKISPNFSIA SAFGNVHGVY KIAAALKPEL LGTFQDYAAK
260 270 280 290 300
QLNKKAEDKP LYLVFHGGSG SSTKDFHTAI DFGVVKVNLD TDCQFAYLSG
310 320 330 340 350
IRDYVLNKKD YLMTPVGNPT GEDSPNKKYY DPRVWVREGE KTMSKRITQA
360
LEIFRTKGAL E
Length:361
Mass (Da):39,600
Last modified:October 1, 2002 - v2
Checksum:iDCF0BEC26FF953D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ272114 Genomic DNA Translation: CAC29023.2
CR382125 Genomic DNA Translation: CAG99377.1
RefSeqiXP_454290.1, XM_454290.1

Genome annotation databases

EnsemblFungiiCAG99377; CAG99377; KLLA0_E07569g
GeneIDi2894094
KEGGikla:KLLA0E07569g

Similar proteinsi

Entry informationi

Entry nameiALF_KLULA
AccessioniPrimary (citable) accession number: Q9C2U0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: October 1, 2002
Last modified: May 23, 2018
This is version 97 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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