Q9C2H9 (SYMM_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable methionine--tRNA ligase, mitochondrial
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

ORF Names:

93G11.130, NCU03759

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 622	622	Probable methionine--tRNA ligase, mitochondrial		PRO_0000139271
Regions
	Motif	67 – 79	13	"HIGH" region
	Motif	366 – 370	5	"KMSKS" region
Sites
	Binding site	369	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9C2H9 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 45E81CBA3ABDAE4E	FASTA	622	70,623
	10 20 30 40 50 60 MDVLRTASAR RTFKWQAAGI LARTSWVCRS CRSQFVAPKA ARRTLATSTT TTTPTAPDNK 70 80 90 100 110 120 PFYVTTPIFY VNASPHVGHM YSMVLGDVLK RWQTLKGNQA ILCTGTDEHG TKVQRAAIAN 130 140 150 160 170 180 DMDPKQFCDL NSAKFQELAA ACRIDYDRFM RTTDQDHVEA VKHFWLLLKE KGLIYEAKHE 190 200 210 220 230 240 GWYCVSDECF YPESQLEKRQ DPFTGEVYVA SIESGNKVEW IEEKNYHFRM TALKDQLLEF 250 260 270 280 290 300 YKNNPDWIVP QTRMNQVVDW VTNNLEDLSI SRPVSRLSWG IRVPDDESQT IYVWVDALIN 310 320 330 340 350 360 YITMAGYPYW PPGREHLGGW PVDVHVIGKD ILRFHCIYWP ALLLALDLPL PKRILSHAHW 370 380 390 400 410 420 TMEKKKMSKS IGNVVNPFYA MERFGIDTMR FYMIHNGGIA NDADYSNDWL TVEYKKHLQN 430 440 450 460 470 480 GVGNLSARVT RPKQWSLRRA VQSYQDGNML LQSTGEQIND SCVEHITRLD KLASTIDNEM 490 500 510 520 530 540 LQLNPSHALQ KIMGLIGETN AFISNAEPWV IVKKQDSEAL VDRIILTAGE SLRIAGILLQ 550 560 570 580 590 600 PFMPDKAAQL LDLLGVENHN RTFHHARPLV DATYGTAFRT FGKEGIFPPP ILDDIKGMGP 610 620 DAGSKKHSSG NKPSSGNKKP TA

References

[1]	"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL513443 Genomic DNA. Translation: CAC28665.1. AABX02000011 Genomic DNA. Translation: EAA31870.1.
RefSeq	XP_961106.1. XM_956013.1.
UniGene	Ncr.15905.
3D structure databases
ProteinModelPortal	Q9C2H9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9C2H9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000003450; EFNCRP00000003450; EFNCRG00000003446.
GeneID	3877271.
KEGG	ncr:NCU03759.
NMPDR	fig|5141.1.peg.994.
Phylogenomic databases
eggNOG	fuNOG04628.
GeneTree	EFGT00050000003465.
OMA	GTYPVFC.
OrthoDB	EOG4TB7KT.
Enzyme and pathway databases
BioCyc	NCRA-XX3-01:NCRA-XX3-01-007507-MONOMER.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR014758. Met-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01874.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. MetG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYMM_NEUCR

Accession

Primary (citable) accession number: Q9C2H9
Secondary accession number(s): Q7RV17

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program