Q9C1W9 (DNLI3_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase 3
EC=6.5.1.1

Alternative name(s):
DNA ligase III
Polydeoxyribonucleotide synthase [ATP] 3

Gene names

Name:	adl1
Synonyms:	lig3
ORF Names:	SPBC713.06

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	774 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Subcellular location	Nucleus Ref.2.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA biosynthetic process Inferred by curator. Source: GeneDB_Spombe DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 774	774	DNA ligase 3		PRO_0000372334
Sites
	Active site	433	1	N6-AMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9C1W9 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 9D69F9EA510F78FF	FASTA	774	87,587
	10 20 30 40 50 60 MPPKKRMKNG SSLKSTSKKG EKSRNIITIQ DLFSKREAQL TDTPNKLLTD HDQSASDYAY 70 80 90 100 110 120 ALKLQQLFDS ENQATAPEKL PKDVIIPEEE YHTDTFNVVK ESNDKPKENL VTSEECKASF 130 140 150 160 170 180 FSTDSVNKDS TIDYDALQKD PLTYVKSCRA RFVSKDTKSF SYSSLANTFS LISSTKSRIR 190 200 210 220 230 240 IVTLLTNFLL TLLYADPDSL IATVWLCTNS IAPNFYGKNL GVGPAMYSKA LKEVCGITAS 250 260 270 280 290 300 ALKNLWNKYG DPGDVAFEAK VSVRTLSRPE PLTIKKVYST LLKIADSNGN GAQNRKLELT 310 320 330 340 350 360 KFLLISSNAE EVRYIGRSIM QNLRIGAVQN TMLASLSKAF FIFDNQNEIF NFNSDSLQQQ 370 380 390 400 410 420 FRQGEEIVKQ SFFQVPDYNI LVATLLREGI ENLKDNMSIR PGIPVKPMLG SITKNLQHML 430 440 450 460 470 480 ERLTDHNFSC EFKYDGQRAQ IHCDRLGNIK IFSRHLEEIT GRFPDVIEVA QLALKHSCDF 490 500 510 520 530 540 IIEGELVAID KSNGQILDFQ KLSTRERKKV TVADITIDVC VFVFDIMFCD GKSCLQMPLI 550 560 570 580 590 600 ERRRMFFEHF NLIPNRFQFV SSLETNEEQS IQEFFSLAIT NKCEGLMVKV LNGTNSKFPS 610 620 630 640 650 660 TYEPDKRGEG WIKVKQDYDD EFESLDLVPI GAWYGNGRKA GWFSPILLAV YNPDTGAYEA 670 680 690 700 710 720 VCKCMSGFSD QFYKELTQKY SLESGNSSLK PIYNFCETGK VTPQIYFAPQ EVWEIKGAQI 730 740 750 760 770 TSSPAYKAAL GLIQDDRGLS IRFPRFIRVR SDKGPEDAST NSILADMYMK QLNT

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAC22607.1.
RefSeq	NP_595345.1. NM_001021253.1.
3D structure databases
HSSP	HSSP built from PDB template 1X9N based on UniProtKB P18858.
ProteinModelPortal	Q9C1W9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9C1W9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC713.06.1; SPBC713.06.1:pep; SPBC713.06.
GeneID	2541111.
GenomeReviews	Gene locus adl1 in contig CU329671_GR.
KEGG	spo:SPBC713.06.
NMPDR	fig|4896.1.peg.1211.
Organism-specific databases
GeneDB_Spombe	SPBC713.06.
Phylogenomic databases
eggNOG	fuNOG06949.
GeneTree	EFGT00050000002597.
HOGENOM	HBG570821.
OMA	EPQEVWE.
OrthoDB	EOG4SFDF2.
Gene expression databases
ArrayExpress	Q9C1W9.
Family and domain databases
InterPro	IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR000014. PAS. [Graphical view]
Gene3D	G3DSA:1.10.3260.10. DNA_ligase_ATP-dep_N. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K10747.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SMART	SM00091. PAS. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. False negative. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI3_SCHPO

Accession

Primary (citable) accession number: Q9C1W9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	June 1, 2001
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program