Reviewed, UniProtKB/Swiss-Prot Q9C1W8 (PPN1_SCHPO)

Names and origin

Protein names

Recommended name:
    Endopolyphosphatase
    EC=3.6.1.10

Gene names

Name:	ppn1
ORF Names:	SPBC713.07c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the hydrolysis of inorganic polyphosphate (poly P) chains of many hundreds of phosphate residues into shorter lengths By similarity.
Catalytic activity	Polyphosphate + n H2O = (n+1) oligophosphate.
Subcellular location	Vacuole membrane; Single-pass type II membrane protein.
Post-translational modification	Processing by proteases in the vacuole may be required for activation By similarity.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular response to stress Inferred from expression pattern. Source: GeneDB_SPombe
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	endopolyphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 577	577	Endopolyphosphatase		PRO_0000058549
Regions
	Topological domain	1 – 2	2	Cytoplasmic Potential
	Transmembrane	3 – 23	21	Signal-anchor for type II membrane protein Potential
	Topological domain	24 – 577	554	Vacuolar Potential
	Compositional bias	436 – 443	8	Poly-Lys
	Compositional bias	446 – 454	9	Poly-Lys
	Compositional bias	550 – 553	4	Poly-Lys
Amino acid modifications
	Glycosylation	363	1	N-linked (GlcNAc...) Potential
	Glycosylation	370	1	N-linked (GlcNAc...) Potential
	Glycosylation	375	1	N-linked (GlcNAc...) Potential
	Glycosylation	399	1	N-linked (GlcNAc...) Potential
	Glycosylation	481	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9C1W8-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: D5EC6913A6E89647	FASTA	577	66,026
	10 20 30 40 50 60 MRPSVITVAV LFVQSTWASF AFGNPMSMRN KAHTNDLVNS KGKPLVGRFL HITDMHPDIY 70 80 90 100 110 120 YEKGSTVDHY CHSYDHNSDD TPLGKSKVGY LSPGPGYECD SSPALIDKTL EWLKEHQDDV 130 140 150 160 170 180 LGGIDFILWT GDNSRHDNDN HFPRTQSEIL ASNEDLVNKM IEAFPDVPIV SAIGNNDIYP 190 200 210 220 230 240 HNIMEAGPSS MTRQLAGAWD ALIPYEERHT FEKGSYYLCD VIPDKLAAIS INTLYLSNKN 250 260 270 280 290 300 AAVDGCPDDN LDEPGSLFMR WFKIQLEAYR LKGMKVWLLG HIPPTRGQWY EDCYTSFTDL 310 320 330 340 350 360 LYEFRDIIVG QLYGHMNINH FVFLEFDKLP VDTESYGIKS AQPKYVKSLI DAQYAELPTF 370 380 390 400 410 420 PENLTEEFLN GTVGNYSLAT VGGSIIPEMF PTFRVYEYNI SDIANQLDDR EELTEITSFN 430 440 450 460 470 480 WETLEEQSQS DYEIDKKKKK KKKNNKKKKK NKRKNIKPGP LGPAYVPSLF TPISFKQYFL 490 500 510 520 530 540 NTSNYMDATK DTEISYELLY TSKDSPYNMP DLTVPEYMRL AKRIATCDKP EDPHQFKLQS 550 560 570 GDQNTFRISK KKKPSICPIA YTYLWHAYIG SISDFED

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAC22608.1.
RefSeq	NP_595346.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2541140.
KEGG	spo:SPBC713.07c.
NMPDR	fig|4896.1.peg.1212.
Organism-specific databases
GeneDB_Spombe	SPBC713.07c.
Phylogenomic databases
OMA	Q9C1W8. AISASHP.
Enzyme and pathway databases
BRENDA	3.6.1.10. 653.
Gene expression databases
ArrayExpress	Q9C1W8.
Family and domain databases
InterPro	IPR012358. EndopolyPtase_N1. IPR004843. M-pesterase. [Graphical view]
PANTHER	PTHR10340:SF4. EndopolyPtase_N1. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF027093. EndopolyPtase_N1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPN1_SCHPO

Accession

Primary (citable) accession number: Q9C1W8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)