Exoglucanase-6A precursor - Humicola insolens (Soft-rot fungus)

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Q9C1S9 (GUX6_HUMIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 52. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Exoglucanase-6A
EC=3.2.1.91

Alternative name(s):
1,4-beta-cellobiohydrolase 6A
Avicelase 2
Beta-glucancellobiohydrolase 6A
Exocellobiohydrolase 6A

Gene names

Name:	cel6A
Synonyms:	avi2

Organism

Humicola insolens (Soft-rot fungus)

Taxonomic identifier

34413 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › mitosporic Ascomycota › Humicola

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a central role in the recycling of plant biomass. The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose. Ref.6
Catalytic activity	Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains. Ref.2
Subunit structure	Monomer. Ref.3 Ref.4 Ref.5 Ref.6
Sequence similarities	Belongs to the glycosyl hydrolase 6 (cellulase A) family. Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular_function	cellulose 1,4-beta-cellobiosidase activity Inferred from electronic annotation. Source: EC cellulose binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 16

Potential

Chain

17 – 476

460

Exoglucanase-6A

PRO_0000248843

Regions

Domain

33 – 60

CBM1

Region

200 – 222

Substrate binding loop 1 Ref.3 Ref.4 Ref.5

Region

423 – 461

Substrate binding loop 2 Ref.3 Ref.4 Ref.5

Compositional bias

73 – 78

Poly-Ser

Sites

Active site

252

Proton donor Ref.3 Ref.5

Active site

431

Proton acceptor Probable Ref.3 Ref.4 Ref.5

Binding site

163

Substrate Ref.3 Ref.4 Ref.5

Binding site

165

Substrate Ref.3 Ref.4 Ref.5

Binding site

297

Substrate Ref.3 Ref.4 Ref.5

Binding site

300

Substrate Ref.3 Ref.4 Ref.5

Binding site

336

Substrate Ref.3 Ref.4 Ref.5

Binding site

397

Substrate Ref.3 Ref.4 Ref.5

Binding site

425

Substrate Ref.3 Ref.4 Ref.5

Binding site

429

Substrate Ref.3 Ref.4 Ref.5

Amino acid modifications

Glycosylation

144

O-linked (Man...) Ref.6

Glycosylation

153

O-linked (Man...) Ref.6

Glycosylation

167

N-linked (GlcNAc...) Ref.3 Ref.4 Ref.5 Ref.6

Disulfide bond

33 ↔ 50

Disulfide bond

44 ↔ 60

Secondary structure

476

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q9C1S9 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: D2B0054F1381E653
Show »

FASTA

476

51,276

        10         20         30         40         50         60 
MAKFFLTAAF AAAALAAPVV EERQNCAPTW GQCGGIGFNG PTCCQSGSTC VKQNDWYSQC 

        70         80         90        100        110        120 
LPGSQVTTTS TTSTSSSSTT SRATSTTRTG GVTSITTAPT RTVTIPGGAT TTASYNGNPF 

       130        140        150        160        170        180 
EGVQLWANNY YRSEVHTLAI PQITDPALRA AASAVAEVPS FQWLDRNVTV DTLLVETLSE 

       190        200        210        220        230        240 
IRAANQAGAN PPYAAQIVVY DLPDRDCAAA ASNGEWAIAN NGANNYKGYI NRIREILISF 

       250        260        270        280        290        300 
SDVRTILVIE PDSLANMVTN MNVAKCSGAA STYRELTIYA LKQLDLPHVA MYMDAGHAGW 

       310        320        330        340        350        360 
LGWPANIQPA AELFAKIYED AGKPRAVRGL ATNVANYNAW SISSPPPYTS PNPNYDEKHY 

       370        380        390        400        410        420 
IEAFRPLLEA RGFPAQFIVD QGRSGKQPTG QKEWGHWCNA IGTGFGMRPT ANTGHQYVDA 

       430        440        450        460        470 
FVWVKPGGEC DGTSDTTAAR YDYHCGLEDA LKPAPEAGQW FQAYFEQLLR NANPPF

« Hide

References

[1]	"Cloning and overexpression of the avi2 gene encoding a major cellulase produced by Humicola insolens FERM BP-5977." Moriya T., Watanabe M., Sumida N., Okakura K., Murakami T. Biosci. Biotechnol. Biochem. 67:1434-1437(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FERM BP-5977.
[2]	"Enzymatic properties of cellulases from Humicola insolens." Schuelein M. J. Biotechnol. 57:71-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY.
[3]	"Structural changes of the active site tunnel of Humicola insolens cellobiohydrolase, Cel6A, upon oligosaccharide binding." Varrot A., Schuelein M., Davies G.J. Biochemistry 38:8884-8891(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 115-476 IN COMPLEX WITH GLUCOSE AND CELLOTETRAOSE, ACTIVE SITE, GLYCOSYLATION AT ASN-167.
[4]	"Structure of the Humicola insolens cellobiohydrolase Cel6A D416A mutant in complex with a non-hydrolysable substrate analogue, methyl cellobiosyl-4-thio-beta-cellobioside, at 1.9 A." Varrot A., Frandsen T.P., Driguez H., Davies G.J. Acta Crystallogr. D 58:2201-2204(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 115-476 OF MUTANT ALA-416 IN COMPLEX WITH SUBSTRATE ANALOG, GLYCOSYLATION AT ASN-167.
[5]	"Structural basis for ligand binding and processivity in cellobiohydrolase Cel6A from Humicola insolens." Varrot A., Frandsen T.P., von Ossowski I., Boyer V., Cottaz S., Driguez H., Schuelein M., Davies G.J. Structure 11:855-864(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.11 ANGSTROMS) OF 113-476 OF MUTANT ASN-431 IN COMPLEX WITH SUBSTRATE ANALOGS, GLYCOSYLATION AT ASN-167.
[6]	"Crystal structure of the catalytic core domain of the family 6 cellobiohydrolase II, Cel6A, from Humicola insolens, at 1.92 A resolution." Varrot A., Hastrup S., Schuelein M., Davies G.J. Biochem. J. 337:297-304(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS) OF 117-476, FUNCTION, SUBUNIT, GLYCOSYLATION AT THR-144; SER-153 AND ASN-167.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB048710 Genomic DNA. Translation: BAB39154.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BVW	X-ray	1.92	A	117-476	[»]
1GZ1	X-ray	1.90	A	117-476	[»]
1OC5	X-ray	1.70	A	115-476	[»]
1OC6	X-ray	1.50	A	115-476	[»]
1OC7	X-ray	1.11	A	115-476	[»]
1OCB	X-ray	1.75	A/B	115-476	[»]
1OCJ	X-ray	1.30	A	115-476	[»]
1OCN	X-ray	1.31	A	115-476	[»]
2BVW	X-ray	1.70	A/B	115-476	[»]

ProteinModelPortal

Q9C1S9.

SMR

Q9C1S9. Positions 30-61, 116-476.

ModBase

Search...

Protein family/group databases

CAZy

CBM1. Carbohydrate-Binding Module Family 1.
GH6. Glycoside Hydrolase Family 6.

mycoCLAP

CBH6A_HUMIN.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.20.20.40. 1 hit.

InterPro

IPR016288. Beta_cellobiohydrolase.
IPR000254. Cellulose-bd_dom_fun.
IPR001524. Glyco_hydro_6_CS.
[Graphical view]

Pfam

PF00734. CBM_1. 1 hit.
PF01341. Glyco_hydro_6. 1 hit.
[Graphical view]

PIRSF

PIRSF001100. Beta_cellobiohydrolase. 1 hit.

PRINTS

PR00733. GLHYDRLASE6.

ProDom

PD001821. CBD_fun. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00236. fCBD. 1 hit.
[Graphical view]

SUPFAM

SSF57180. CBD_fun. 1 hit.
SSF51989. Glyco_hydro_6. 1 hit.

PROSITE

PS00562. CBM1_1. 1 hit.
PS51164. CBM1_2. 1 hit.
PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9C1S9.

Entry information

Entry name

GUX6_HUMIN

Accession

Primary (citable) accession number: Q9C1S9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Family and domain databases

Other

Entry information

Relevant documents