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Protein

Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase

Gene

alg-3

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc(2)-PP-Dol.By similarity

Catalytic activityi

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT58. Glycosyltransferase Family 58.

Names & Taxonomyi

Protein namesi
Recommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase (EC:2.4.1.258)
Alternative name(s):
Asparagine-linked glycosylation protein 6
Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
Gene namesi
Name:alg-3
ORF Names:NCU06552
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 4, Linkage Group IV

Organism-specific databases

EuPathDBiFungiDB:NCU06552.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3434LumenalSequence analysisAdd
BLAST
Transmembranei35 – 5521HelicalSequence analysisAdd
BLAST
Topological domaini56 – 8429CytoplasmicSequence analysisAdd
BLAST
Transmembranei85 – 10521HelicalSequence analysisAdd
BLAST
Topological domaini106 – 1116LumenalSequence analysis
Transmembranei112 – 13221HelicalSequence analysisAdd
BLAST
Topological domaini133 – 15523CytoplasmicSequence analysisAdd
BLAST
Transmembranei156 – 17621HelicalSequence analysisAdd
BLAST
Topological domaini177 – 19822LumenalSequence analysisAdd
BLAST
Transmembranei199 – 21921HelicalSequence analysisAdd
BLAST
Topological domaini220 – 2201CytoplasmicSequence analysis
Transmembranei221 – 24121HelicalSequence analysisAdd
BLAST
Topological domaini242 – 27231LumenalSequence analysisAdd
BLAST
Transmembranei273 – 29321HelicalSequence analysisAdd
BLAST
Topological domaini294 – 33340CytoplasmicSequence analysisAdd
BLAST
Transmembranei334 – 35421HelicalSequence analysisAdd
BLAST
Topological domaini355 – 37622LumenalSequence analysisAdd
BLAST
Transmembranei377 – 39721HelicalSequence analysisAdd
BLAST
Topological domaini398 – 4014CytoplasmicSequence analysis
Transmembranei402 – 42221HelicalSequence analysisAdd
BLAST
Topological domaini423 – 44220LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 442442Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferasePRO_0000350929Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the ALG3 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000237555.
InParanoidiQ9C1K8.
KOiK03845.
OMAiDWKAYMD.
OrthoDBiEOG092C26UZ.

Family and domain databases

InterProiIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERiPTHR12646. PTHR12646. 1 hit.
PfamiPF05208. ALG3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9C1K8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPSSRPES NPPLYKQALD FALDVANGRH ALSKLIPPAL FLVDALLCGL
60 70 80 90 100
IIWKVPYTEI DWAAYMEQVS QILSGERDYT KVRGGTGPLV YPAAHVYIYT
110 120 130 140 150
GLYHLTDEGR NILLAQQLFA GLYMVTLAVV MGCYWQAKAP PYLFPLLTLS
160 170 180 190 200
KRLHSIFVLR CFNDCFAVLF LWLAIFFFQR RNWQAGALLY TLGLGVKMTL
210 220 230 240 250
LLSLPAVGIV LFLGSGSFVT TLQLVATMGL VQILIGVPFL AHYPTEYLSR
260 270 280 290 300
AFELSRQFFF KWTVNWRFVG EEIFLSKGFA LTLLALHVLV LGIFITTRWI
310 320 330 340 350
KPARKSLVQL ISPVLLAGKP PLTVPEHRAA ARDVTPRYIM TTILSANAVG
360 370 380 390 400
LLFARSLHYQ FYAYVAWSTP FLLWRAGLHP VLVYLLWAVH EWAWNVFPST
410 420 430 440
PASSAVVVGV LGVTVAGVWF GAREEWEPGM KSSSKKEEAA MR
Length:442
Mass (Da):49,480
Last modified:June 1, 2001 - v1
Checksum:iA42F627206CC73D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF309689 Genomic DNA. Translation: AAK07848.1.
CM002239 Genomic DNA. Translation: EAA33023.1.
RefSeqiXP_962259.1. XM_957166.2.

Genome annotation databases

EnsemblFungiiEFNCRT00000006403; EFNCRP00000006395; EFNCRG00000006392.
GeneIDi3878417.
KEGGincr:NCU06552.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF309689 Genomic DNA. Translation: AAK07848.1.
CM002239 Genomic DNA. Translation: EAA33023.1.
RefSeqiXP_962259.1. XM_957166.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT58. Glycosyltransferase Family 58.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFNCRT00000006403; EFNCRP00000006395; EFNCRG00000006392.
GeneIDi3878417.
KEGGincr:NCU06552.

Organism-specific databases

EuPathDBiFungiDB:NCU06552.

Phylogenomic databases

HOGENOMiHOG000237555.
InParanoidiQ9C1K8.
KOiK03845.
OMAiDWKAYMD.
OrthoDBiEOG092C26UZ.

Enzyme and pathway databases

UniPathwayiUPA00378.

Family and domain databases

InterProiIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
PANTHERiPTHR12646. PTHR12646. 1 hit.
PfamiPF05208. ALG3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG3_NEUCR
AccessioniPrimary (citable) accession number: Q9C1K8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.