Reviewed, UniProtKB/Swiss-Prot Q9C1K8 (ALG3_NEUCR)

Names and origin

Protein names

Recommended name:
    Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
    EC=2.4.1.130
Alternative name(s):
    Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
    Asparagine-linked glycosylation protein 6

Gene names

Name:	alg-3
ORF Names:	NCU06552

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc(2)-PP-Dol By similarity.
Catalytic activity	Transfers an alpha-D-mannosyl residue from dolichyl-phosphate D-mannose into membrane lipid-linked oligosaccharide.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the ALG3 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase		PRO_0000350929
Regions
	Topological domain	1 – 34	34	Lumenal Potential
	Transmembrane	35 – 55	21	Potential
	Topological domain	56 – 84	29	Cytoplasmic Potential
	Transmembrane	85 – 105	21	Potential
	Topological domain	106 – 111	6	Lumenal Potential
	Transmembrane	112 – 132	21	Potential
	Topological domain	133 – 155	23	Cytoplasmic Potential
	Transmembrane	156 – 176	21	Potential
	Topological domain	177 – 198	22	Lumenal Potential
	Transmembrane	199 – 219	21	Potential
	Topological domain	220	1	Cytoplasmic Potential
	Transmembrane	221 – 241	21	Potential
	Topological domain	242 – 272	31	Lumenal Potential
	Transmembrane	273 – 293	21	Potential
	Topological domain	294 – 333	40	Cytoplasmic Potential
	Transmembrane	334 – 354	21	Potential
	Topological domain	355 – 376	22	Lumenal Potential
	Transmembrane	377 – 397	21	Potential
	Topological domain	398 – 401	4	Cytoplasmic Potential
	Transmembrane	402 – 422	21	Potential
	Topological domain	423 – 442	20	Lumenal Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9C1K8-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: A42F627206CC73D2	FASTA	442	49,480
	10 20 30 40 50 60 MAAPSSRPES NPPLYKQALD FALDVANGRH ALSKLIPPAL FLVDALLCGL IIWKVPYTEI 70 80 90 100 110 120 DWAAYMEQVS QILSGERDYT KVRGGTGPLV YPAAHVYIYT GLYHLTDEGR NILLAQQLFA 130 140 150 160 170 180 GLYMVTLAVV MGCYWQAKAP PYLFPLLTLS KRLHSIFVLR CFNDCFAVLF LWLAIFFFQR 190 200 210 220 230 240 RNWQAGALLY TLGLGVKMTL LLSLPAVGIV LFLGSGSFVT TLQLVATMGL VQILIGVPFL 250 260 270 280 290 300 AHYPTEYLSR AFELSRQFFF KWTVNWRFVG EEIFLSKGFA LTLLALHVLV LGIFITTRWI 310 320 330 340 350 360 KPARKSLVQL ISPVLLAGKP PLTVPEHRAA ARDVTPRYIM TTILSANAVG LLFARSLHYQ 370 380 390 400 410 420 FYAYVAWSTP FLLWRAGLHP VLVYLLWAVH EWAWNVFPST PASSAVVVGV LGVTVAGVWF 430 440 GAREEWEPGM KSSSKKEEAA MR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the pdx-1 (snz-1/sno-1) region of the Neurospora crassa genome. Correlation of pyridoxine-requiring phenotypes with mutations in two structural genes." Bean L.E., Dvorachek W.H. Jr., Braun E.L., Errett A., Saenz G.S., Giles M.D., Werner-Washburne M., Nelson M.A., Natvig D.O. Genetics 157:1067-1075(2001) [PubMed: 11238395] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF309689 Genomic DNA. Translation: AAK07848.1. AABX02000028 Genomic DNA. Translation: EAA33023.1.
RefSeq	XP_962259.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT58. Glycosyltransferase Family 58.
Genome annotation databases
GeneID	3878417.
KEGG	ncr:NCU06552.
Family and domain databases
InterPro	IPR007873. Glycosyltransferase_ALG3. [Graphical view]
PANTHER	PTHR12646. ALG3. 1 hit.
Pfam	PF05208. ALG3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG3_NEUCR

Accession

Primary (citable) accession number: Q9C1K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)