Q9C136 (G3P1_RHIRA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 1
Short name=GAPDH 1
EC=1.2.1.12

Gene names

Name:

GPD1

Organism

Rhizomucor racemosus (Mucor circinelloides f. lusitanicus)

Taxonomic identifier

4841 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Mucor

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Induction	Highly expressed under anaerobic growth conditions in the presence of glucose and the expression is highly regulated in response to carbon source and/or anaerobiosis. Ref.1
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Glyceraldehyde-3-phosphate dehydrogenase 1		PRO_0000145576
Regions
	Nucleotide binding	12 – 13	2	NAD By similarity
	Region	150 – 152	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	210 – 211	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	151	1	Nucleophile By similarity
	Binding site	34	1	NAD By similarity
	Binding site	79	1	NAD; via carbonyl oxygen By similarity
	Binding site	181	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	233	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	315	1	NAD By similarity
	Site	178	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9C136 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 74441099459B79A7	FASTA	337	35,340
	10 20 30 40 50 60 MVVQVGINGF GRIGRIVLRA TESNKDVQVV AINDPFIPLD YMVYMLKYDT VHGRFDGSVE 70 80 90 100 110 120 AKDGKLVVNG HAIAVSAERD PTSIPWGSAG ADYVVESTGV FTTTEAASAH LKGGAKKVII 130 140 150 160 170 180 SAPSADAPMF VCGVNLEAYK SEYKVISNAS CTTNCLAPLA KVINDNFGIA DGLMTTVHAT 190 200 210 220 230 240 TATQKTVDGP SHKDWRGGRA AAANIIPSST GAAKAVGKVI PALNGKLTGM AFRVPTPDVS 250 260 270 280 290 300 VVDLTVNLSK GASYDEIKQA IKKASETTMK GVLGYTSDAV VSSDFVGEVC SSVFDAAAGI 310 320 330 QLTPTFVKLI AWYDNEYGYS NRVVDLLVHA AKVDGAL

References

[1]	"Cloning of glyceraldehyde-3-phosphate dehydrogenase-encoding genes in Mucor circinelloides (Syn. racemosus) and use of the gpd1 promoter for recombinant protein production." Wolff A.M., Arnau J. Fungal Genet. Biol. 35:21-29(2002) [PubMed: 11860262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION. Strain: ATCC 90680 / R7B.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ293012 Genomic DNA. Translation: CAC37403.1.
3D structure databases
ProteinModelPortal	Q9C136.
SMR	Q9C136. Positions 4-336.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P1_RHIRA

Accession

Primary (citable) accession number: Q9C136

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	June 1, 2001
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program