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Protein

Minichromosome loss protein 1

Gene

mcl1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in regulating DNA replication complexes. Acts as a regulator of post DNA replication initiation. Associates with chromatin during G1 and S phases of mitosis. Required for the transcriptional repression of the outer repeats of the centromeric region. Acts as a polymerase alpha replication accessory factor and is important for S-phase DNA damage survival. Plays a role in lagging-strand synthesis and Ozaki fragment processing, in addition to DNA repair.4 Publications

GO - Biological processi

  • cellular response to DNA damage stimulus Source: PomBase
  • chromatin silencing at centromere Source: PomBase
  • chromatin silencing at centromere central core Source: PomBase
  • DNA repair Source: UniProtKB-KW
  • Okazaki fragment processing involved in mitotic DNA replication Source: PomBase
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Minichromosome loss protein 1
Alternative name(s):
DNA polymerase alpha accessory factor Mcl1
Gene namesi
Name:mcl1
Synonyms:slr3
ORF Names:SPAPB1E7.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAPB1E7.02c.
PomBaseiSPAPB1E7.02c. mcl1.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric core domain Source: GOC
  • nuclear chromatin Source: PomBase
  • nuclear chromosome Source: PomBase
  • nucleoplasm Source: PomBase
  • nucleus Source: PomBase
  • protein complex Source: PomBase
  • replication fork Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 815815Minichromosome loss protein 1PRO_0000051073Add
BLAST

Proteomic databases

MaxQBiQ9C107.

Interactioni

Subunit structurei

Interacts with pof3 and pol1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
pof3O749912EBI-1203666,EBI-1153554
pol1P2804010EBI-1203666,EBI-455823

Protein-protein interaction databases

BioGridi279809. 261 interactions.
IntActiQ9C107. 3 interactions.
MINTiMINT-4702222.

Structurei

3D structure databases

ProteinModelPortaliQ9C107.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati11 – 5040WD 1Add
BLAST
Repeati53 – 9038WD 2Add
BLAST
Repeati93 – 13240WD 3Add
BLAST
Repeati135 – 17440WD 4Add
BLAST
Repeati228 – 26740WD 5Add
BLAST
Repeati517 – 55337WD 6Add
BLAST

Sequence similaritiesi

Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000209337.
InParanoidiQ9C107.
KOiK11274.
OMAiEWTYELP.
OrthoDBiEOG7W41N8.
PhylomeDBiQ9C107.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR022100. Mcl1_mid.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF12341. Mcl1_mid. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9C107-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGNRLVPRY AHTDGLTRLA YTRDGKFLLT VGSNQVIRKF QVGSDEEPDS
60 70 80 90 100
IDNHQDPITG IAVAENYFCT CSEDATVCVY PIDSPTEHTL LARTTLPIRD
110 120 130 140 150
VAYSVDGNWI AIASDETAVK VVSSTDSSQI FSLRPAKASN KHVTYSPNGN
160 170 180 190 200
FLAVSSCNGI LYFYDTQTRE LIKFLTNTIA SLEAESEICS KAAWHPKNGT
210 220 230 240 250
FAVASTDHFV SVISPDDWLP LYKLLPKENH SGVTDISWSS NGMYIAASFK
260 270 280 290 300
KGGILIWDTQ SHEVVVELPY STVVALAWQP FENVLSFTTN QGILYSCPDV
310 320 330 340 350
IPKSILKEEN DPTKPLTSSK SKNRTSKELD DLFGSDDEQS QNVNDLDGNS
360 370 380 390 400
ANEENEFINH DGLDSSLDLD GDSYMVDEND LNLAKKRKQK ALIDRTTTIE
410 420 430 440 450
NGSSKRRLLQ ASIHKPVHTG STPWQGNRRY LCLNLVGFIW TVQQDAEHNT
460 470 480 490 500
ITVEFHDETT HRKYHFVDDQ KFEMACLDHE GALYASPATE SSPGVIYYKA
510 520 530 540 550
HVDWSRKSEW AMALPMENES PVTISLSSSV VLVCTSAGYV RVFSRQGFPI
560 570 580 590 600
SIHRSKHLPF VACSSFQDTI ITIANDGLSS DGNSRLVYSI EDISRDEMLQ
610 620 630 640 650
TGDGVALPPQ GTLESVFFSD VGDPYIYDST GVLLVLMHWR IPGQAKWIPV
660 670 680 690 700
LDTNELERRK SRQESYWPVT VADNQFHCIL LKGASRYPYF PRPMFTEFDF
710 720 730 740 750
RIPCNTNNPD ASTSVPVLEE LQLRNKLFLT LLEDSIGDGD VTEDEKISIA
760 770 780 790 800
RLEANIDKAL LQLIQKACLE ERIERVYELT KTLRRTTSIA AAQKIALHHS
810
LTNVAEKIGN LLSNV
Length:815
Mass (Da):90,986
Last modified:June 1, 2001 - v1
Checksum:iD3AD3EF2D7997C54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAC36919.1.
RefSeqiNP_594128.1. NM_001019552.2.

Genome annotation databases

EnsemblFungiiSPAPB1E7.02c.1; SPAPB1E7.02c.1:pep; SPAPB1E7.02c.
GeneIDi2543387.
KEGGispo:SPAPB1E7.02c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAC36919.1.
RefSeqiNP_594128.1. NM_001019552.2.

3D structure databases

ProteinModelPortaliQ9C107.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279809. 261 interactions.
IntActiQ9C107. 3 interactions.
MINTiMINT-4702222.

Proteomic databases

MaxQBiQ9C107.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAPB1E7.02c.1; SPAPB1E7.02c.1:pep; SPAPB1E7.02c.
GeneIDi2543387.
KEGGispo:SPAPB1E7.02c.

Organism-specific databases

EuPathDBiFungiDB:SPAPB1E7.02c.
PomBaseiSPAPB1E7.02c. mcl1.

Phylogenomic databases

HOGENOMiHOG000209337.
InParanoidiQ9C107.
KOiK11274.
OMAiEWTYELP.
OrthoDBiEOG7W41N8.
PhylomeDBiQ9C107.

Miscellaneous databases

PROiQ9C107.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR022100. Mcl1_mid.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF12341. Mcl1_mid. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "mcl1+, the Schizosaccharomyces pombe homologue of CTF4, is important for chromosome replication, cohesion, and segregation."
    Williams D.R., McIntosh J.R.
    Eukaryot. Cell 1:758-773(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Genetic and physical interactions between Schizosaccharomyces pombe Mcl1 and Rad2, Dna2 and DNA polymerase alpha: evidence for a multifunctional role of Mcl1 in DNA replication and repair."
    Tsutsui Y., Morishita T., Natsume T., Yamashita K., Iwasaki H., Yamao F., Shinagawa H.
    Curr. Genet. 48:34-43(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH POL1.
  4. "Mcl1p is a polymerase alpha replication accessory factor important for S-phase DNA damage survival."
    Williams D.R., McIntosh J.R.
    Eukaryot. Cell 4:166-177(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH POL1.
  5. "Fission yeast Mcl1 interacts with SCF(Pof3) and is required for centromere formation."
    Mamnun Y.M., Katayama S., Toda T.
    Biochem. Biophys. Res. Commun. 350:125-130(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH POF3.
  6. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMCL1_SCHPO
AccessioniPrimary (citable) accession number: Q9C107
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.