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Q9C0Y4

- AGLU_SCHPO

UniProt

Q9C0Y4 - AGLU_SCHPO

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Protein

Alpha-glucosidase

Gene
agl1, agl, SPAPB24D3.10c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes malto-oligosaccharides, but has a low activity toward soluble starch.

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei481 – 4811Nucleophile
Active sitei484 – 4841
Active sitei647 – 6471Proton donor

GO - Molecular functioni

  1. alpha-glucosidase activity Source: PomBase
  2. carbohydrate binding Source: InterPro
  3. maltose alpha-glucosidase activity Source: PomBase
  4. starch alpha-glucosidase activity Source: PomBase

GO - Biological processi

  1. cellular polysaccharide catabolic process Source: PomBase
  2. maltose catabolic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.
mycoCLAPiAGL31A_SCHPO.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-glucosidase (EC:3.2.1.20)
Alternative name(s):
Maltase
Gene namesi
Name:agl1
Synonyms:agl
ORF Names:SPAPB24D3.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAPB24D3.10c.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi218 – 2181D → N: Almost total loss of activity. 1 Publication
Mutagenesisi287 – 2871D → N: No loss of activity. 1 Publication
Mutagenesisi355 – 3551D → E or N: Almost total loss of activity. 1 Publication
Mutagenesisi481 – 4811D → A, E or N: Loss of activity. 1 Publication
Mutagenesisi484 – 4841E → A or Q: Loss of activity. 1 Publication
Mutagenesisi484 – 4841E → D: No loss of activity. 1 Publication
Mutagenesisi647 – 6471D → A, E or N: Loss of activity. 1 Publication
Mutagenesisi676 – 6761D → N: No loss of activity. 1 Publication
Mutagenesisi714 – 7141E → Q: No loss of activity. 1 Publication
Mutagenesisi877 – 8771D → N: Almost total loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Chaini25 – 969945Alpha-glucosidasePRO_0000018581Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi37 – 371N-linked (GlcNAc...) Reviewed prediction
Glycosylationi67 – 671N-linked (GlcNAc...) Reviewed prediction
Glycosylationi99 – 991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi116 – 1161N-linked (GlcNAc...) Reviewed prediction
Glycosylationi139 – 1391N-linked (GlcNAc...) Reviewed prediction
Glycosylationi146 – 1461N-linked (GlcNAc...) Reviewed prediction
Glycosylationi209 – 2091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi245 – 2451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi249 – 2491N-linked (GlcNAc...) Reviewed prediction
Glycosylationi331 – 3311N-linked (GlcNAc...) Reviewed prediction
Glycosylationi406 – 4061N-linked (GlcNAc...) Reviewed prediction
Glycosylationi429 – 4291N-linked (GlcNAc...) Reviewed prediction
Glycosylationi462 – 4621N-linked (GlcNAc...) Reviewed prediction
Glycosylationi470 – 4701N-linked (GlcNAc...) Reviewed prediction
Glycosylationi520 – 5201N-linked (GlcNAc...) Reviewed prediction
Glycosylationi523 – 5231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi589 – 5891N-linked (GlcNAc...) Reviewed prediction
Glycosylationi648 – 6481N-linked (GlcNAc...) Reviewed prediction
Glycosylationi801 – 8011N-linked (GlcNAc...) Reviewed prediction
Glycosylationi810 – 8101N-linked (GlcNAc...) Reviewed prediction
Glycosylationi821 – 8211N-linked (GlcNAc...) Reviewed prediction
Glycosylationi885 – 8851N-linked (GlcNAc...) Reviewed prediction
Glycosylationi915 – 9151N-linked (GlcNAc...) Reviewed prediction
Glycosylationi934 – 9341N-linked (GlcNAc...) Reviewed prediction
Glycosylationi942 – 9421N-linked (GlcNAc...) Reviewed prediction
Glycosylationi954 – 9541N-linked (GlcNAc...) Reviewed prediction
Glycosylationi966 – 9661N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9C0Y4.

Interactioni

Protein-protein interaction databases

MINTiMINT-4702058.
STRINGi4896.SPAPB24D3.10c-1.

Structurei

3D structure databases

ProteinModelPortaliQ9C0Y4.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1501.
HOGENOMiHOG000041175.
OrthoDBiEOG77T1CZ.
PhylomeDBiQ9C0Y4.

Family and domain databases

InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9C0Y4-1 [UniParc]FASTAAdd to Basket

« Hide

MMISTAYQSL FLTALFSAIS IAVGNVYQTL NVIGDRNVTI PTNGIPQRLS    50
VYDPYRGVNC QGYQAVNISE SQNGVTAYLA LLGEPCYAYG TDYPLLFLNV 100
TYEEADRVHI SIKDANNTQF QFTSRKDLWD APLYSPSYNN TNLLYNFSYN 150
ANPFEFWVTR KSDGEVLFDT RGQKLVFEDQ YIELTTNMVE NYNLYGLAET 200
IHGLRLGNNL TRTFWANDEP SPVDQNMYGS HPYYLEQRYK ADGINSTLNE 250
TTYTSSSHGV LMLTANGMDV LLRQDYLQYR MIGGVIDLFV YSGSTESPKE 300
TVKQFVQSIG KPAMHQYWTL GYHSCRWGYT NITEIMDVRQ NYIDADIPVE 350
TFWSDIDYME KYRDFTVDPV SYSKSDMQTF FSDLVSNHQH YVPIIDAAIY 400
AANPYNHTDD SYYPYYAGVE KDIFLKNPNG SIYIGAVWPG FTAFPDFTNP 450
DVVDYWKDCL INLTYAFGSN GTVPFSGIWT DMNEPSSFCV GSCGSAMIDL 500
NPAEPLTGIS KQYSIPEGFN VSNVTEYSSA YSASLSNYYA TATSSVFQIV 550
SPTATPLGLK PDYNIDWPPY AINNEQGNHD IANHIVSPNA TTHDGTQRYD 600
IFNMYGYGET KVSYAALTQI SPNERPFILS RSTFLGSGVY GAHWLGDNHS 650
LWSNMFFSIS GMIVFNMMGI PMVGADVCGF LGDSDEELCS RWMAMGAFSP 700
FYRNHNNIYQ ISQEPYTWSS VAEASRRAMY IRYSLLPYWY TIMAKASQDG 750
TPALRALFVE FPNDPTLADV DRQFMVGDSL LVTPVLEPNV EYVQGVFPGD 800
NSTVWYDWYN HTEIVRQYNE NVTLYAPLEH INVAIRGGSV LPMQQPSLTT 850
YESRQNPFNL LVALDRDGSA TGELYLDDGV SIELNATLSV SFTFSDGVLS 900
AVPTGSYEVS QPLANVTILG LTESPSSITL NGQNVSSFQY SNDTEELLIT 950
GLQNITSSGA FANSWNLTL 969
Length:969
Mass (Da):108,686
Last modified:August 29, 2001 - v2
Checksum:iF3122E2CFA551C25
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301L → F in CAC36906. 1 Publication
Sequence conflicti220 – 2201P → A in BAB43946. 1 Publication
Sequence conflicti507 – 5071T → V in BAB43946. 1 Publication
Sequence conflicti566 – 5661D → N in BAB43946. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAC36906.1.
AB045751 mRNA. Translation: BAB43946.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CU329670 Genomic DNA. Translation: CAC36906.1 .
AB045751 mRNA. Translation: BAB43946.1 .

3D structure databases

ProteinModelPortali Q9C0Y4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4702058.
STRINGi 4896.SPAPB24D3.10c-1.

Protein family/group databases

CAZyi GH31. Glycoside Hydrolase Family 31.
mycoCLAPi AGL31A_SCHPO.

Proteomic databases

MaxQBi Q9C0Y4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

PomBasei SPAPB24D3.10c.

Phylogenomic databases

eggNOGi COG1501.
HOGENOMi HOG000041175.
OrthoDBi EOG77T1CZ.
PhylomeDBi Q9C0Y4.

Miscellaneous databases

NextBioi 20804509.

Family and domain databases

InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF01055. Glyco_hydro_31. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Carboxyl group of residue Asp647 as possible proton donor in catalytic reaction of alpha-glucosidase from Schizosaccharomyces pombe."
    Okuyama M., Okuno A., Shimizu N., Mori H., Kimura A., Chiba S.
    Eur. J. Biochem. 268:2270-2280(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 25-36; 175-194; 375-395 AND 427-451, MUTAGENESIS OF ASP-218; ASP-287; ASP-355; ASP-481; GLU-484; ASP-647; ASP-676; GLU-714 AND ASP-877.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiAGLU_SCHPO
AccessioniPrimary (citable) accession number: Q9C0Y4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: August 29, 2001
Last modified: September 3, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi