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Protein

Kinetochore protein mis15

Gene

mis15

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The Sim4 complex is required for loading the DASH complex onto the kinetochore via interaction with dad1. Has a role in the maintenance of core chromatin structure and kinetochore function. Located in the inner regions of the centromeres and is essential for sister chromatid segregation during the G1/S phase following mitosis. Required for correct chromosome segregation where it has a role in the formation and/or maintenance of specialized chromatin at the centromere.4 Publications

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • CENP-A containing chromatin organization Source: PomBase
  • centromere complex assembly Source: InterPro
  • mitotic sister chromatid segregation Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein mis15
Alternative name(s):
Sim4 complex subunit mis15
Gene namesi
Name:mis15
ORF Names:pi022, SPBP22H7.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

PomBaseiSPBP22H7.09c. mis15.

Subcellular locationi

GO - Cellular componenti

  • condensed chromosome, centromeric region Source: PomBase
  • condensed nuclear chromosome kinetochore Source: PomBase
  • Mis6-Sim4 complex Source: PomBase
  • mitotic spindle pole body Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 409409Kinetochore protein mis15PRO_0000116774Add
BLAST

Proteomic databases

MaxQBiQ9C0W0.

Interactioni

Subunit structurei

Component of the Sim4 complex which consists of sim4, mal2, mis6, mis15, mis17, fta1, fta2, fta3, fta4, fta5, fta6 and fta7. Interacts with mis6 and mis17.4 Publications

Protein-protein interaction databases

BioGridi277833. 9 interactions.
IntActiQ9C0W0. 2 interactions.
MINTiMINT-4701879.

Family & Domainsi

Phylogenomic databases

InParanoidiQ9C0W0.
KOiK11551.
OMAiDCQLIVD.
OrthoDBiEOG7DVDMF.
PhylomeDBiQ9C0W0.

Family and domain databases

InterProiIPR027715. CENP-N.
IPR007902. Chl4/mis15/CENP-N.
[Graphical view]
PANTHERiPTHR12309:SF12. PTHR12309:SF12. 1 hit.
PfamiPF05238. CENP-N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9C0W0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTELSSNIR IKHDKKIQKL LNRFPRDFLV KLCVEWIQKQ TYPPNAKDIN
60 70 80 90 100
LEDMLDDEEW NPEAFYKNVP KSMLKRSIIH RMLVYDWPNG FYLGQIAQLE
110 120 130 140 150
ILALAHGFVS MRWTASKVHH SAEKTVLPNP LVFLELLKSE LESIFVYHTY
160 170 180 190 200
ISRHETLPIT FIRLVLWDSK RPTALHTYPS SKQIFYLGLM DDSDVLLHNI
210 220 230 240 250
FLRNDVCHSL FLQCLSRLLY RLKAGSALRP IDLVSKNLTT FCTNVGVNKE
260 270 280 290 300
ANALGAWQIY AKNLVDRSPL DTRPILSDDN SSLIADSTQN CEKHREMAIQ
310 320 330 340 350
RRFGDTHSQV LDKLLITLDH DYIEKSTEKD KVLEENVESY NPTEQRPLVV
360 370 380 390 400
MQLRGQHILE GLKDICRQDA LDPFTMPSYL TGETGLSILY VRDGLVLQRQ

ESLGQQEIN
Length:409
Mass (Da):47,394
Last modified:March 23, 2010 - v3
Checksum:i60938B407FB66897
GO

Sequence cautioni

The sequence BAA21401.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA21401.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004535 Genomic DNA. Translation: BAA21401.1. Sequence problems.
CU329671 Genomic DNA. Translation: CAM11335.2.
RefSeqiXP_001713131.2. XM_001713079.2.

Genome annotation databases

EnsemblFungiiSPBP22H7.09c.1; SPBP22H7.09c.1:pep; SPBP22H7.09c.
GeneIDi2541321.
KEGGispo:SPBP22H7.09c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004535 Genomic DNA. Translation: BAA21401.1. Sequence problems.
CU329671 Genomic DNA. Translation: CAM11335.2.
RefSeqiXP_001713131.2. XM_001713079.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277833. 9 interactions.
IntActiQ9C0W0. 2 interactions.
MINTiMINT-4701879.

Proteomic databases

MaxQBiQ9C0W0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBP22H7.09c.1; SPBP22H7.09c.1:pep; SPBP22H7.09c.
GeneIDi2541321.
KEGGispo:SPBP22H7.09c.

Organism-specific databases

PomBaseiSPBP22H7.09c. mis15.

Phylogenomic databases

InParanoidiQ9C0W0.
KOiK11551.
OMAiDCQLIVD.
OrthoDBiEOG7DVDMF.
PhylomeDBiQ9C0W0.

Miscellaneous databases

NextBioi20802431.
PROiQ9C0W0.

Family and domain databases

InterProiIPR027715. CENP-N.
IPR007902. Chl4/mis15/CENP-N.
[Graphical view]
PANTHERiPTHR12309:SF12. PTHR12309:SF12. 1 hit.
PfamiPF05238. CENP-N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A 38 kb segment containing the cdc2 gene from the left arm of fission yeast chromosome II: sequence analysis and characterization of the genomic DNA and cDNAs encoded on the segment."
    Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K., Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y., Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M.
    Yeast 16:71-80(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres."
    Hayashi T., Fujita Y., Iwasaki O., Adachi Y., Takahashi K., Yanagida M.
    Cell 118:715-729(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MIS6 AND MIS17, SUBCELLULAR LOCATION, REVISION OF GENE MODEL.
  4. "Molecular analysis of kinetochore architecture in fission yeast."
    Liu X., McLeod I., Anderson S., Yates J.R. III, He X.
    EMBO J. 24:2919-2930(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE SIM4 COMPLEX.
  5. "Two distinct pathways responsible for the loading of CENP-A to centromeres in the fission yeast cell cycle."
    Takahashi K., Takayama Y., Masuda F., Kobayashi Y., Saitoh S.
    Philos. Trans. R. Soc. Lond., B, Biol. Sci. 360:595-606(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Fta2, an essential fission yeast kinetochore component, interacts closely with the conserved Mal2 protein."
    Kerres A., Jakopec V., Beuter C., Karig I., Pohlmann J., Pidoux A., Allshire R., Fleig U.
    Mol. Biol. Cell 17:4167-4178(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE SIM4 COMPLEX.
  7. "Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe."
    Hayashi A., Asakawa H., Haraguchi T., Hiraoka Y.
    Mol. Biol. Cell 17:5173-5184(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. "Mis17 is a regulatory module of the Mis6-Mal2-Sim4 centromere complex that is required for the recruitment of CenH3/CENP-A in fission yeast."
    Shiroiwa Y., Hayashi T., Fujita Y., Villar-Briones A., Ikai N., Takeda K., Ebe M., Yanagida M.
    PLoS ONE 6:E17761-E17761(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SIM4 COMPLEX.

Entry informationi

Entry nameiMIS15_SCHPO
AccessioniPrimary (citable) accession number: Q9C0W0
Secondary accession number(s): A1IU53, O13613
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: March 23, 2010
Last modified: April 13, 2016
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.