Q9C0K7 (STRAB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
STE20-related kinase adapter protein beta
Short name=STRAD beta

Alternative name(s):
Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein
CALS-21
ILP-interacting protein
Pseudokinase ALS2CR2

Gene names

Name:	STRADB
Synonyms:	ALS2CR2, ILPIP
ORF Names:	PRO1038

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation By similarity. Ref.7
Subunit structure	Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity. Interacts with BIRC4/XIAP. These two proteins are likely to coexist in a complex with TAK1, TRAF6, TAB1 and TAB2. Ref.2 Ref.7
Subcellular location	Nucleus. Cytoplasm Ref.7.
Tissue specificity	Highly expressed in heart, skeletal muscle, testis, liver and colon. Ref.1 Ref.2
Domain	The protein kinase domain is predicted to be catalytically inactive.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 protein kinase domain.
Caution	Ser-184 is present instead of the conserved Asp which is expected to be an active site residue.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	JNK cascade Inferred from electronic annotation. Source: Compara activation of protein kinase activity Inferred from direct assay Ref.7. Source: UniProtKB cell cycle arrest Traceable author statement. Source: Reactome cell morphogenesis Inferred from electronic annotation. Source: Compara insulin receptor signaling pathway Traceable author statement. Source: Reactome negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara protein export from nucleus Inferred from direct assay Ref.7. Source: UniProtKB
Cellular_component	cytosol Traceable author statement. Source: Reactome nucleus Inferred from direct assay Ref.7. Source: UniProtKB
Molecular_function	ATP binding Non-traceable author statement. Source: UniProtKB transferase activity, transferring phosphorus-containing groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAB39	Q9Y376	5	EBI-306893,EBI-306905
STK11	Q15831	6	EBI-306893,EBI-306838

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9C0K7-1)

Also known as: ILPIP-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9C0K7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     372-377: MKEESQ → PYFEFL
     378-418: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9C0K7-3)

Also known as: ILPIP-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-138: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	STE20-related kinase adapter protein beta		PRO_0000085617
Regions
	Domain	58 – 369	312	Protein kinase
	Nucleotide binding	64 – 72	9	ATP By similarity
Sites
	Binding site	89	1	ATP By similarity
Natural variations
	Alternative sequence	1 – 138	138	Missing in isoform 3.		VSP_016625
	Alternative sequence	372 – 377	6	MKEESQ → PYFEFL in isoform 2.		VSP_016623
	Alternative sequence	378 – 418	41	Missing in isoform 2.		VSP_016624
	Natural variant	155	1	G → E in a metastatic melanoma sample; somatic mutation. Ref.8		VAR_041335
	Natural variant	386	1	P → L. Ref.8 Corresponds to variant rs35636836 [ dbSNP | Ensembl ].		VAR_041336

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (ILPIP-alpha) [UniParc]. Last modified June 1, 2001. Version 1. Checksum: A63ED6CCE1E18C96	FASTA	418	47,026
	10 20 30 40 50 60 MSLLDCFCTS RTQVESLRPE KQSETSIHQY LVDEPTLSWS RPSTRASEVL CSTNVSHYEL 70 80 90 100 110 120 QVEIGRGFDN LTSVHLARHT PTGTLVTIKI TNLENCNEER LKALQKAVIL SHFFRHPNIT 130 140 150 160 170 180 TYWTVFTVGS WLWVISPFMA YGSASQLLRT YFPEGMSETL IRNILFGAVR GLNYLHQNGC 190 200 210 220 230 240 IHRSIKASHI LISGDGLVTL SGLSHLHSLV KHGQRHRAVY DFPQFSTSVQ PWLSPELLRQ 250 260 270 280 290 300 DLHGYNVKSD IYSVGITACE LASGQVPFQD MHRTQMLLQK LKGPPYSPLD ISIFPQSESR 310 320 330 340 350 360 MKNSQSGVDS GIGESVLVSS GTHTVNSDRL HTPSSKTFSP AFFSLVQLCL QQDPEKRPSA 370 380 390 400 410 SSLLSHVFFK QMKEESQDSI LSLLPPAYNK PSISLPPVLP WTEPECDFPD EKDSYWEF
	Isoform 2 [UniParc]. Checksum: C869F81FDED0839A	FASTA	377	42,371
	10 20 30 40 50 60 MSLLDCFCTS RTQVESLRPE KQSETSIHQY LVDEPTLSWS RPSTRASEVL CSTNVSHYEL 70 80 90 100 110 120 QVEIGRGFDN LTSVHLARHT PTGTLVTIKI TNLENCNEER LKALQKAVIL SHFFRHPNIT 130 140 150 160 170 180 TYWTVFTVGS WLWVISPFMA YGSASQLLRT YFPEGMSETL IRNILFGAVR GLNYLHQNGC 190 200 210 220 230 240 IHRSIKASHI LISGDGLVTL SGLSHLHSLV KHGQRHRAVY DFPQFSTSVQ PWLSPELLRQ 250 260 270 280 290 300 DLHGYNVKSD IYSVGITACE LASGQVPFQD MHRTQMLLQK LKGPPYSPLD ISIFPQSESR 310 320 330 340 350 360 MKNSQSGVDS GIGESVLVSS GTHTVNSDRL HTPSSKTFSP AFFSLVQLCL QQDPEKRPSA 370 SSLLSHVFFK QPYFEFL
	Isoform 3 (ILPIP-beta) [UniParc]. Checksum: 9D433EAA77D5CF08	FASTA	280	31,219
	10 20 30 40 50 60 MAYGSASQLL RTYFPEGMSE TLIRNILFGA VRGLNYLHQN GCIHRSIKAS HILISGDGLV 70 80 90 100 110 120 TLSGLSHLHS LVKHGQRHRA VYDFPQFSTS VQPWLSPELL RQDLHGYNVK SDIYSVGITA 130 140 150 160 170 180 CELASGQVPF QDMHRTQMLL QKLKGPPYSP LDISIFPQSE SRMKNSQSGV DSGIGESVLV 190 200 210 220 230 240 SSGTHTVNSD RLHTPSSKTF SPAFFSLVQL CLQQDPEKRP SASSLLSHVF FKQMKEESQD 250 260 270 280 SILSLLPPAY NKPSISLPPV LPWTEPECDF PDEKDSYWEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of three novel genes, ALS2CR1, ALS2CR2, and ALS2CR3, in the juvenile amyotrophic lateral sclerosis (ALS2) critical region at chromosome 2q33-q34: candidate genes for ALS2." Hadano S., Yanagisawa Y., Skaug J., Fichter K., Nasir J., Martindale D., Koop B.F., Scherer S.W., Nicholson D.W., Rouleau G.A., Ikeda J.-E., Hayden M.R. Genomics 71:200-213(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]	"ILPIP, a novel anti-apoptotic protein that enhances XIAP-mediated activation of JNK1 and protection against apoptosis." Sanna M.G., Da Silva Correia J., Luo Y., Chuang B., Paulson L.M., Nguyen B., Deveraux Q.L., Ulevitch R.J. J. Biol. Chem. 277:30454-30462(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), TISSUE SPECIFICITY, INTERACTION WITH BIRC4.
[3]	"Functional prediction of the coding sequences of 121 new genes deduced by analysis of cDNA clones from human fetal liver." Zhang C., Yu Y., Zhang S., Wei H., Zhou G., Ouyang S., Luo L., Bi J., Liu M., He F. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Fetal liver.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Placenta.
[7]	"MO25alpha/beta interact with STRADalpha/beta enhancing their ability to bind, activate and localize LKB1 in the cytoplasm." Boudeau J., Baas A.F., Deak M., Morrice N.A., Kieloch A., Schutkowski M., Prescott A.R., Clevers H.C., Alessi D.R. EMBO J. 22:5102-5114(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH STK11/LKB1 AND CAB39.
[8]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] GLU-155 AND LEU-386.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB038950 mRNA. Translation: BAB32500.1. AY093697 mRNA. Translation: AAM19143.1. AF116618 mRNA. Translation: AAF71042.1. AK027637 mRNA. Translation: BAB55254.1. AC007282 Genomic DNA. Translation: AAY14692.1. BC008302 mRNA. Translation: AAH08302.1. BC090871 mRNA. Translation: AAH90871.1.
IPI	IPI00032099. IPI00657901. IPI00658041.
RefSeq	NP_001193793.1. NM_001206864.1. NP_061041.2. NM_018571.5.
UniGene	Hs.652338.
3D structure databases
ProteinModelPortal	Q9C0K7.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9C0K7. 4 interactions.
MINT	MINT-7969373.
STRING	9606.ENSP00000194530.
PTM databases
PhosphoSite	Q9C0K7.
Polymorphism databases
DMDM	74762722.
Proteomic databases
PaxDb	Q9C0K7.
PRIDE	Q9C0K7.
Protocols and materials databases
DNASU	55437.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000194530; ENSP00000194530; ENSG00000082146. ENST00000392249; ENSP00000376080; ENSG00000082146. ENST00000539670; ENSP00000443981; ENSG00000082146.
GeneID	55437.
KEGG	hsa:55437.
UCSC	uc002uyd.4. human. uc021vvb.1. human.
Organism-specific databases
CTD	55437.
GeneCards	GC02P202252.
HGNC	HGNC:13205. STRADB.
HPA	HPA026549. HPA028520.
MIM	607333. gene.
neXtProt	NX_Q9C0K7.
PharmGKB	PA24743.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000005157.
HOVERGEN	HBG055069.
InParanoid	Q9C0K7.
OMA	QYLVDEP.
PhylomeDB	Q9C0K7.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction. REACT_11163. Activated AMPK stimulates fatty-acid oxidation in muscle.
SignaLink	Q9C0K7.
Gene expression databases
ArrayExpress	Q9C0K7.
Bgee	Q9C0K7.
CleanEx	HS_STRADB.
Genevestigator	Q9C0K7.
GermOnline	ENSG00000082146. Homo sapiens.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	55437.
NextBio	59838.
SOURCE	Search...

Entry information

Entry name

STRAB_HUMAN

Accession

Primary (citable) accession number: Q9C0K7
Secondary accession number(s): Q5BKY7, Q9P1L0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	June 1, 2001
Last modified:	May 29, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.