Q9C0J8 (WDR33_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
pre-mRNA 3' end processing protein WDR33

Alternative name(s):
WD repeat-containing protein 33
WD repeat-containing protein WDC146

Gene names

Name:	WDR33
Synonyms:	WDC146

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for both cleavage and polyadenylation of pre-mRNA 3' ends. Ref.11
Subunit structure	Component of the cleavage and polyadenylation specificity factor (CPSF) module of the pre-mRNA 3' end processing complex. Interacts with CPSF3/CPSF73. Ref.11
Subcellular location	Nucleus Ref.1 Ref.11.
Tissue specificity	Most highly expressed in testis. Ref.1
Sequence similarities	Contains 1 collagen-like domain. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Collagen Repeat WD repeat
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	postreplication repair Non-traceable author statement Ref.1. Source: UniProtKB spermatogenesis Non-traceable author statement Ref.1. Source: UniProtKB
Cellular component	collagen Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9C0J8-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9C0J8-2) The sequence of this isoform differs from the canonical sequence as follows:      209-326: SFSPTDNKFA...IRNLKEELQV → RFIHNIPFSV...YFIPNKEFSL      327-1336: Missing.
Sequence annotation (Features)
	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
	Sequence conflict	274	1	R → Q in BAD97039. Ref.3
	Sequence conflict	306	1	F → S in AAH05401. Ref.6

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 1336	1335	pre-mRNA 3' end processing protein WDR33		PRO_0000051382
Regions
	Repeat	117 – 156	40	WD 1
	Repeat	159 – 198	40	WD 2
	Repeat	200 – 239	40	WD 3
	Repeat	242 – 283	42	WD 4
	Repeat	286 – 325	40	WD 5
	Repeat	329 – 369	41	WD 6
	Repeat	373 – 412	40	WD 7
	Domain	618 – 770	153	Collagen-like
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.10
	Modified residue	7	1	Phosphoserine Ref.8 Ref.10
	Modified residue	46	1	N6-acetyllysine Ref.12
	Modified residue	1210	1	Phosphoserine Ref.9
Natural variations
	Alternative sequence	209 – 326	118	SFSPT…EELQV → RFIHNIPFSVVPIVMVKLFS KCILGAEMHGLCQFLGNFLH PINTIFFFVFTHSPFCWHLS EVVLSRYQPLQYVRDVLSAA FCTGFLFSFMINNVYTLFLF IIYCVRQEYFIPNKEFSL in isoform 2.		VSP_041333
	Alternative sequence	327 – 1336	1010	Missing in isoform 2.		VSP_041334
	Natural variant	33	1	A → S. Corresponds to variant rs11557686 [ dbSNP | Ensembl ].		VAR_046717
	Natural variant	711	1	P → R. Corresponds to variant rs12615078 [ dbSNP | Ensembl ].		VAR_053427
Experimental info
	Sequence conflict	113	1	T → A in BAD97039. Ref.3
	Sequence conflict	715	1	G → S in BAB32435. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 14, 2008. Version 2. Checksum: DE45F510F93BB783	FASTA	1,336	145,891
	10 20 30 40 50 60 MATEIGSPPR FFHMPRFQHQ APRQLFYKRP DFAQQQAMQQ LTFDGKRMRK AVNRKTIDYN 70 80 90 100 110 120 PSVIKYLENR IWQRDQRDMR AIQPDAGYYN DLVPPIGMLN NPMNAVTTKF VRTSTNKVKC 130 140 150 160 170 180 PVFVVRWTPE GRRLVTGASS GEFTLWNGLT FNFETILQAH DSPVRAMTWS HNDMWMLTAD 190 200 210 220 230 240 HGGYVKYWQS NMNNVKMFQA HKEAIREASF SPTDNKFATC SDDGTVRIWD FLRCHEERIL 250 260 270 280 290 300 RGHGADVKCV DWHPTKGLVV SGSKDSQQPI KFWDPKTGQS LATLHAHKNT VMEVKLNLNG 310 320 330 340 350 360 NWLLTASRDH LCKLFDIRNL KEELQVFRGH KKEATAVAWH PVHEGLFASG GSDGSLLFWH 370 380 390 400 410 420 VGVEKEVGGM EMAHEGMIWS LAWHPLGHIL CSGSNDHTSK FWTRNRPGDK MRDRYNLNLL 430 440 450 460 470 480 PGMSEDGVEY DDLEPNSLAV IPGMGIPEQL KLAMEQEQMG KDESNEIEMT IPGLDWGMEE 490 500 510 520 530 540 VMQKDQKKVP QKKVPYAKPI PAQFQQAWMQ NKVPIPAPNE VLNDRKEDIK LEEKKKTQAE 550 560 570 580 590 600 IEQEMATLQY TNPQLLEQLK IERLAQKQVE QIQPPPSSGT PLLGPQPFPG QGPMSQIPQG 610 620 630 640 650 660 FQQPHPSQQM PMNMAQMGPP GPQGQFRPPG PQGQMGPQGP PLHQGGGGPQ GFMGPQGPQG 670 680 690 700 710 720 PPQGLPRPQD MHGPQGMQRH PGPHGPLGPQ GPPGPQGSSG PQGHMGPQGP PGPQGHIGPQ 730 740 750 760 770 780 GPPGPQGHLG PQGPPGTQGM QGPPGPRGMQ GPPHPHGIQG GPGSQGIQGP VSQGPLMGLN 790 800 810 820 830 840 PRGMQGPPGP RENQGPAPQG MIMGHPPQEM RGPHPPGGLL GHGPQEMRGP QEIRGMQGPP 850 860 870 880 890 900 PQGSMLGPPQ ELRGPPGSQS QQGPPQGSLG PPPQGGMQGP PGPQGQQNPA RGPHPSQGPI 910 920 930 940 950 960 PFQQQKTPLL GDGPRAPFNQ EGQSTGPPPL IPGLGQQGAQ GRIPPLNPGQ GPGPNKGDSR 970 980 990 1000 1010 1020 GPPNHHMGPM SERRHEQSGG PEHGPERGPF RGGQDCRGPP DRRGPHPDFP DDFSRPDDFH 1030 1040 1050 1060 1070 1080 PDKRFGHRLR EFEGRGGPLP QEEKWRRGGP GPPFPPDHRE FSEGDGRGAA RGPPGAWEGR 1090 1100 1110 1120 1130 1140 RPGDERFPRD PEDPRFRGRR EESFRRGAPP RHEGRAPPRG RDGFPGPEDF GPEENFDASE 1150 1160 1170 1180 1190 1200 EAARGRDLRG RGRGTPRGGR KGLLPTPDEF PRFEGGRKPD SWDGNREPGP GHEHFRDTPR 1210 1220 1230 1240 1250 1260 PDHPPHDGHS PASRERSSSL QGMDMASLPP RKRPWHDGPG TSEHREMEAP GGPSEDRGGK 1270 1280 1290 1300 1310 1320 GRGGPGPAQR VPKSGRSSSL DGEHHDGYHR DEPFGGPPGS GTPSRGGRSG SNWGRGSNMN 1330 SGPPRRGASR GGGRGR
	Isoform 2 [UniParc]. Checksum: 1F1F704B877F7904	FASTA	326	38,294
	10 20 30 40 50 60 MATEIGSPPR FFHMPRFQHQ APRQLFYKRP DFAQQQAMQQ LTFDGKRMRK AVNRKTIDYN 70 80 90 100 110 120 PSVIKYLENR IWQRDQRDMR AIQPDAGYYN DLVPPIGMLN NPMNAVTTKF VRTSTNKVKC 130 140 150 160 170 180 PVFVVRWTPE GRRLVTGASS GEFTLWNGLT FNFETILQAH DSPVRAMTWS HNDMWMLTAD 190 200 210 220 230 240 HGGYVKYWQS NMNNVKMFQA HKEAIREARF IHNIPFSVVP IVMVKLFSKC ILGAEMHGLC 250 260 270 280 290 300 QFLGNFLHPI NTIFFFVFTH SPFCWHLSEV VLSRYQPLQY VRDVLSAAFC TGFLFSFMIN 310 320 NVYTLFLFII YCVRQEYFIP NKEFSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel WD40 repeat protein, WDC146, highly expressed during spermatogenesis in a stage-specific manner." Ito S., Sakai A., Nomura T., Miki Y., Ouchida M., Sasaki J., Shimizu K. Biochem. Biophys. Res. Commun. 280:656-663(2001) [PubMed: 11162572] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Placenta.
[3]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Small intestine.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain and Pancreas.
[7]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1187-1336 (ISOFORM 1). Tissue: Melanoma.
[8]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1210, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[11]	"Molecular architecture of the human pre-mRNA 3' processing complex." Shi Y., Di Giammartino D.C., Taylor D., Sarkeshik A., Rice W.J., Yates J.R. III, Frank J., Manley J.L. Mol. Cell 33:365-376(2009) [PubMed: 19217410] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE 3' PRE-MRNA END PROCESSING COMPLEX, SUBCELLULAR LOCATION, INTERACTION WITH CPSF3.
[12]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-46, MASS SPECTROMETRY.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB044749 mRNA. Translation: BAB32435.1. AK002156 mRNA. Translation: BAA92113.1. AK223319 mRNA. Translation: BAD97039.1. AC006011 Genomic DNA. Translation: AAX82033.1. CH471103 Genomic DNA. Translation: EAW95342.1. CH471103 Genomic DNA. Translation: EAW95343.1. BC005401 mRNA. Translation: AAH05401.1. Different termination. BC013990 mRNA. Translation: AAH13990.1. AL834365 mRNA. Translation: CAH10688.1.
IPI	IPI00106567. IPI00385811.
RefSeq	NP_001006623.1. NM_001006622.2. NP_060853.3. NM_018383.4.
UniGene	Hs.554831.
3D structure databases
ProteinModelPortal	Q9C0J8.
SMR	Q9C0J8. Positions 117-411.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9C0J8. 13 interactions.
PTM databases
PhosphoSite	Q9C0J8.
Polymorphism databases
DMDM	209572695.
Proteomic databases
PRIDE	Q9C0J8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000322313; ENSP00000325377; ENSG00000136709.
GeneID	55339.
KEGG	hsa:55339.
Organism-specific databases
CTD	55339.
GeneCards	GC02M128557.
HGNC	HGNC:25651. WDR33.
HPA	HPA026897.
neXtProt	NX_Q9C0J8.
PharmGKB	PA134943440.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07493.
GeneTree	ENSGT00540000070914.
HOGENOM	HBG125697.
HOVERGEN	HBG054623.
InParanoid	Q9C0J8.
OMA	GGPQGFM.
OrthoDB	EOG4V6ZG7.
PhylomeDB	Q9C0J8.
Gene expression databases
ArrayExpress	Q9C0J8.
Bgee	Q9C0J8.
CleanEx	HS_WDR33.
Genevestigator	Q9C0J8.
GermOnline	ENSG00000136709. Homo sapiens.
Family and domain databases
InterPro	IPR008160. Collagen. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
KO	K15542.
Pfam	PF01391. Collagen. 2 hits. PF00400. WD40. 6 hits. [Graphical view]
SMART	SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. 6 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WDR33_HUMAN

Accession

Primary (citable) accession number: Q9C0J8
Secondary accession number(s): Q05DP8 , Q53FG9, Q587J1, Q69YF7, Q9NUL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.