Q9C0E4 (GRIP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate receptor-interacting protein 2
Short name=GRIP-2

Gene names

Name:	GRIP2
Synonyms:	KIAA1719

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1043 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons By similarity.
Subunit structure	Interacts with EFNB1, EFNB3, GRIA2, GRIA3, CSPG4 and GRIPAP1. Can form homomultimers and heteromultimers with GRIP1 By similarity. Interacts with the C-terminal tail of PRLHR. Ref.5
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Domain	PDZ 5 mediates the C-terminal binding of GRIA2 and GRIA3. PDZ 6 mediates interaction with the PDZ recognition motif of EFNB1. PDZ 7 mediates interaction with CSPG4 By similarity.
Sequence similarities	Belongs to the GRIP2 family. Contains 7 PDZ (DHR) domains.
Sequence caution	The sequence BAB21810.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	synaptic transmission Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome plasma membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9C0E4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9C0E4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     858-873: SPAPGPAREEGFWRMF → RHPVSPLSTPSHLPLF
     874-1043: Missing.

Note: Due to intron retention. No experimental confirmation available.

Isoform 3 (identifier: Q9C0E4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MLCGLSRETPGE → QEWKRKWNSAFPSFSP
     135-170: APENNPRIISKTVDVSLYKEGNSFGFVLRGGAHEDG → GGCPWTGNFLHCLGCGWTRLPPHQGLGGRDHARWSG
     171-1043: Missing.

Note: Incomplete sequence. Due to intron retention. No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1043

1043

Glutamate receptor-interacting protein 2

PRO_0000083852

Regions

Domain

48 – 131

84

PDZ 1

Domain

148 – 234

87

PDZ 2

Domain

248 – 332

85

PDZ 3

Domain

456 – 545

90

PDZ 4

Domain

557 – 641

85

PDZ 5

Domain

656 – 738

83

PDZ 6

Domain

941 – 1023

83

PDZ 7

Natural variations

Alternative sequence

1 – 12

12

MLCGL…ETPGE → QEWKRKWNSAFPSFSP in isoform 3.

VSP_009754

Alternative sequence

135 – 170

36

APENN…AHEDG → GGCPWTGNFLHCLGCGWTRL PPHQGLGGRDHARWSG in isoform 3.

VSP_009755

Alternative sequence

171 – 1043

873

Missing in isoform 3.

VSP_009756

Alternative sequence

858 – 873

16

SPAPG…FWRMF → RHPVSPLSTPSHLPLF in isoform 2.

VSP_009757

Alternative sequence

874 – 1043

170

Missing in isoform 2.

VSP_009758

Experimental info

Sequence conflict

850

1

G → E Ref.4

Secondary structure

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1043

Helix Strand Turn

Details...

Beta strand

243 – 252

10

Beta strand

262 – 266

5

Beta strand

269 – 271

3

Beta strand

273 – 278

6

Helix

284 – 288

5

Beta strand

296 – 300

5

Helix

310 – 318

9

Beta strand

321 – 328

8

Beta strand

451 – 459

9

Turn

463 – 465

3

Beta strand

469 – 472

4

Beta strand

477 – 480

4

Beta strand

486 – 490

5

Helix

495 – 498

4

Beta strand

509 – 511

3

Beta strand

514 – 516

3

Helix

521 – 532

12

Turn

533 – 535

3

Beta strand

537 – 543

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 29, 2004. Version 3. Checksum: 26E384310D706B1F	FASTA	1,043	112,501
	10 20 30 40 50 60 MLCGLSRETP GEADDGPYSK GGKDAGGADV SLACRRQSIP EEFRGITVVE LIKKEGSTLG 70 80 90 100 110 120 LTISGGTDKD GKPRVSNLRP GGLAARSDLL NIGDYIRSVN GIHLTRLRHD EIITLLKNVG 130 140 150 160 170 180 ERVVLEVEYE LPPPAPENNP RIISKTVDVS LYKEGNSFGF VLRGGAHEDG HKSRPLVLTY 190 200 210 220 230 240 VRPGGPADRE GSLKVGDRLL SVDGIPLHGA SHATALATLR QCSHEALFQV EYDVATPDTV 250 260 270 280 290 300 ANASGPLMVE IVKTPGSALG ISLTTTSLRN KSVITIDRIK PASVVDRSGA LHPGDHILSI 310 320 330 340 350 360 DGTSMEHCSL LEATKLLASI SEKVRLEILP VPQSQRPLRP SEAVKVQRSE QLHRWDPCVP 370 380 390 400 410 420 SCHSPRPGHC RMPTWATPAG QDQSRSLSST PFSSPTLNHA FSCNNPSTLP RGSQPMSPRT 430 440 450 460 470 480 TMGRRRQRRR EHKSSLSLAS STVGPGGQIV HTETTEVVLC GDPLSGFGLQ LQGGIFATET 490 500 510 520 530 540 LSSPPLVCFI EPDSPAERCG LLQVGDRVLS INGIATEDGT MEEANQLLRD AALAHKVVLE 550 560 570 580 590 600 VEFDVAESVI PSSGTFHVKL PKKRSVELGI TISSASRKRG EPLIISDIKK GSVAHRTGTL 610 620 630 640 650 660 EPGDKLLAID NIRLDNCPME DAVQILRQCE DLVKLKIRKD EDNSDELETT GAVSYTVELK 670 680 690 700 710 720 RYGGPLGITI SGTEEPFDPI VISGLTKRGL AERTGAIHVG DRILAINNVS LKGRPLSEAI 730 740 750 760 770 780 HLLQVAGETV TLKIKKQLDR PLLPRKSGSL SETSDADEDP ADALKGGLPA ARFSPAVPSV 790 800 810 820 830 840 DSAVESWDSS ATEGGFGGPG SYTPQAAARG TTPQERRPGW LRGSPPPTEP RRTSYTPTPA 850 860 870 880 890 900 DESFPEEEEG DDWEPPTSPA PGPAREEGFW RMFGEALEDL ESCGQSELLR ELEASIMTGT 910 920 930 940 950 960 VQRVALEGRP GHRPWQRGRE VRASPAEMEE LLLPTPLEMH KVTLHKDPMR HDFGFSVSDG 970 980 990 1000 1010 1020 LLEKGVYVHT VRPDGPAHRG GLQPFDRVLQ VNHVRTRDFD CCLAVPLLAE AGDVLELIIS 1030 1040 RKPHTAHSSR APRSPGPSSP RML
	Isoform 2 [UniParc]. Checksum: 46682AF4EC2D544E	FASTA	873	93,624
	10 20 30 40 50 60 MLCGLSRETP GEADDGPYSK GGKDAGGADV SLACRRQSIP EEFRGITVVE LIKKEGSTLG 70 80 90 100 110 120 LTISGGTDKD GKPRVSNLRP GGLAARSDLL NIGDYIRSVN GIHLTRLRHD EIITLLKNVG 130 140 150 160 170 180 ERVVLEVEYE LPPPAPENNP RIISKTVDVS LYKEGNSFGF VLRGGAHEDG HKSRPLVLTY 190 200 210 220 230 240 VRPGGPADRE GSLKVGDRLL SVDGIPLHGA SHATALATLR QCSHEALFQV EYDVATPDTV 250 260 270 280 290 300 ANASGPLMVE IVKTPGSALG ISLTTTSLRN KSVITIDRIK PASVVDRSGA LHPGDHILSI 310 320 330 340 350 360 DGTSMEHCSL LEATKLLASI SEKVRLEILP VPQSQRPLRP SEAVKVQRSE QLHRWDPCVP 370 380 390 400 410 420 SCHSPRPGHC RMPTWATPAG QDQSRSLSST PFSSPTLNHA FSCNNPSTLP RGSQPMSPRT 430 440 450 460 470 480 TMGRRRQRRR EHKSSLSLAS STVGPGGQIV HTETTEVVLC GDPLSGFGLQ LQGGIFATET 490 500 510 520 530 540 LSSPPLVCFI EPDSPAERCG LLQVGDRVLS INGIATEDGT MEEANQLLRD AALAHKVVLE 550 560 570 580 590 600 VEFDVAESVI PSSGTFHVKL PKKRSVELGI TISSASRKRG EPLIISDIKK GSVAHRTGTL 610 620 630 640 650 660 EPGDKLLAID NIRLDNCPME DAVQILRQCE DLVKLKIRKD EDNSDELETT GAVSYTVELK 670 680 690 700 710 720 RYGGPLGITI SGTEEPFDPI VISGLTKRGL AERTGAIHVG DRILAINNVS LKGRPLSEAI 730 740 750 760 770 780 HLLQVAGETV TLKIKKQLDR PLLPRKSGSL SETSDADEDP ADALKGGLPA ARFSPAVPSV 790 800 810 820 830 840 DSAVESWDSS ATEGGFGGPG SYTPQAAARG TTPQERRPGW LRGSPPPTEP RRTSYTPTPA 850 860 870 DESFPEEEEG DDWEPPTRHP VSPLSTPSHL PLF
	Isoform 3 [UniParc]. Checksum: A76F1C4FF8B51B74	FASTA	174	18,938
	10 20 30 40 50 60 QEWKRKWNSA FPSFSPADDG PYSKGGKDAG GADVSLACRR QSIPEEFRGI TVVELIKKEG 70 80 90 100 110 120 STLGLTISGG TDKDGKPRVS NLRPGGLAAR SDLLNIGDYI RSVNGIHLTR LRHDEIITLL 130 140 150 160 170 KNVGERVVLE VEYELPPPGG CPWTGNFLHC LGCGWTRLPP HQGLGGRDHA RWSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O. DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"The nucleotide sequence of a long cDNA clone isolated from human spleen." Jikuya H., Takano J., Nomura N., Kikuno R., Nagase T., Ohara O. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Spleen.
[4]	"Characterization of long cDNA clones from human adult spleen." Hattori A., Okumura K., Nagase T., Kikuno R., Hirosawa M., Ohara O. DNA Res. 7:357-366(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 543-1043 (ISOFORM 2). Tissue: Spleen.
[5]	"The carboxyl terminus of the prolactin-releasing peptide receptor interacts with PDZ domain proteins involved in alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor clustering." Lin S.H.S., Arai A.C., Wang Z., Nothacker H.-P., Civelli O. Mol. Pharmacol. 60:916-923(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PRLHR.
[6]	"Solution structure of the 3rd and the 4th PDZ domain of GRIP2." RIKEN structural genomics initiative (RSGI) Submitted (MAY-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 238-341 AND 446-544.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB051506 mRNA. Translation: BAB21810.2. Different initiation.
AK074145 mRNA. Translation: BAB84971.1.
AK024507 mRNA. Translation: BAB15797.1.

IPI

IPI00180230.
IPI00383056.
IPI00737140.

RefSeq

NP_001073892.2. NM_001080423.2.

UniGene

Hs.517819.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1V62	NMR	-	A	238-341	[»]
1X5R	NMR	-	A	446-544	[»]

ProteinModelPortal

Q9C0E4.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9C0E4. 4 interactions.

MINT

MINT-1785790.

STRING

9606.ENSP00000392703.

PTM databases

PhosphoSite

Q9C0E4.

Polymorphism databases

DMDM

48474948.

Proteomic databases

PaxDb

Q9C0E4.

PRIDE

Q9C0E4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

80852.

KEGG

hsa:80852.

UCSC

uc003byv.1. human.
uc021wtn.1. human.

Organism-specific databases

CTD

80852.

GeneCards

GC03M014505.

H-InvDB

HIX0003085.

HGNC

HGNC:23841. GRIP2.

neXtProt

NX_Q9C0E4.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG297474.

HOVERGEN

HBG051841.

InParanoid

Q9C0E4.

Enzyme and pathway databases

Reactome

REACT_13685. Neuronal System.

Gene expression databases

CleanEx

HS_GRIP2.

Genevestigator

Q9C0E4.

GermOnline

ENSG00000144596. Homo sapiens.

Family and domain databases

InterPro

IPR001478. PDZ.
[Graphical view]

Pfam

PF00595. PDZ. 7 hits.
[Graphical view]

SMART

SM00228. PDZ. 7 hits.
[Graphical view]

SUPFAM

SSF50156. PDZ. 7 hits.

PROSITE

PS50106. PDZ. 7 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9C0E4.

GenomeRNAi

80852.

NextBio

71288.

Entry information

Entry name

GRIP2_HUMAN

Accession

Primary (citable) accession number: Q9C0E4
Secondary accession number(s): Q8TEH9, Q9H7H3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	March 29, 2004
Last modified:	May 1, 2013
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.