Q9C0C9 (UBE2O_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 O
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein O
Ubiquitin-conjugating enzyme E2 of 230 kDa
Short name=Ubiquitin-conjugating enzyme E2-230K
Ubiquitin-protein ligase O

Gene names

Name:	UBE2O
Synonyms:	KIAA1734

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1292 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Tissue specificity	Predominantly expressed in skeletal muscle and heart. Ref.7
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.
Sequence caution	The sequence AAH22237.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH25977.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH36820.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH51868.2 differs from that shown. Reason: Erroneous initiation. The sequence BAB14320.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14948.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB15313.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB21825.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Polymorphism
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1292	1292	Ubiquitin-conjugating enzyme E2 O		PRO_0000280637
Regions
	Coiled coil	812 – 882	71	Potential
	Compositional bias	2 – 43	42	Ala-rich
Sites
	Active site	1040	1	Glycyl thioester intermediate By similarity
Amino acid modifications
	Modified residue	87	1	Phosphoserine Ref.10 Ref.11
	Modified residue	89	1	Phosphoserine Ref.10 Ref.11
	Modified residue	399	1	Phosphoserine Ref.10 Ref.11
	Modified residue	401	1	Phosphoserine Ref.10 Ref.11
	Modified residue	435	1	Phosphothreonine Ref.10
	Modified residue	441	1	Phosphoserine Ref.10
	Modified residue	836	1	Phosphoserine Ref.10 Ref.11 Ref.12
	Modified residue	838	1	Phosphothreonine Ref.9 Ref.10 Ref.11
	Modified residue	839	1	Phosphoserine Ref.9 Ref.11
	Modified residue	841	1	Phosphothreonine Ref.9
	Modified residue	896	1	Phosphoserine Ref.8
Natural variations
	Natural variant	1207	1	G → S. Ref.1 Ref.4 Ref.5 Ref.6 Corresponds to variant rs3803739 [ dbSNP | Ensembl ].		VAR_031184
Experimental info
	Sequence conflict	50	1	S → F in BAB21825. Ref.1
	Sequence conflict	675	1	E → EQ in BAB15313. Ref.6
	Sequence conflict	1047	1	W → C in AAH22237. Ref.4
	Sequence conflict	1143	1	E → D in AAH25977. Ref.4
	Sequence conflict	1150	1	A → T in BAB14320. Ref.6
	Sequence conflict	1261	1	L → P in BAB14320. Ref.6
	Sequence conflict	1290	1	E → G in BAB14948. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q9C0C9 [UniParc]. Last modified October 14, 2008. Version 3. Checksum: C9DF6395A32E9AB0	FASTA	1,292	141,293
	10 20 30 40 50 60 MADPAAPTPA APAPAQAPAP APEAVPAPAA APVPAPAPAS DSASGPSSDS GPEAGSQRLL 70 80 90 100 110 120 FSHDLVSGRY RGSVHFGLVR LIHGEDSDSE GEEEGRGSSG CSEAGGAGHE EGRASPLRRG 130 140 150 160 170 180 YVRVQWYPEG VKQHVKETKL KLEDRSVVPR DVVRHMRSTD SQCGTVIDVN IDCAVKLIGT 190 200 210 220 230 240 NCIIYPVNSK DLQHIWPFMY GDYIAYDCWL GKVYDLKNQI ILKLSNGARC SMNTEDGAKL 250 260 270 280 290 300 YDVCPHVSDS GLFFDDSYGF YPGQVLIGPA KIFSSVQWLS GVKPVLSTKS KFRVVVEEVQ 310 320 330 340 350 360 VVELKVTWIT KSFCPGGTDS VSPPPSVITQ ENLGRVKRLG CFDHAQRQLG ERCLYVFPAK 370 380 390 400 410 420 VEPAKIAWEC PEKNCAQGEG SMAKKVKRLL KKQVVRIMSC SPDTQCSRDH SMEDPDKKGE 430 440 450 460 470 480 SKTKSEAESA SPEETPDGSA SPVEMQDEGA EEPHEAGEQL PPFLLKEGRD DRLHSAEQDA 490 500 510 520 530 540 DDEAADDTDD TSSVTSSASS TTSSQSGSGT SRKKSIPLSI KNLKRKHKRK KNKITRDFKP 550 560 570 580 590 600 GDRVAVEVVT TMTSADVMWQ DGSVECNIRS NDLFPVHHLD NNEFCPGDFV VDKRVQSCPD 610 620 630 640 650 660 PAVYGVVQSG DHIGRTCMVK WFKLRPSGDD VELIGEEEDV SVYDIADHPD FRFRTTDIVI 670 680 690 700 710 720 RIGNTEDGAP HKEDEPSVGQ VARVDVSSKV EVVWADNSKT IILPQHLYNI ESEIEESDYD 730 740 750 760 770 780 SVEGSTSGAS SDEWEDDSDS WETDNGLVED EHPKIEEPPI PPLEQPVAPE DKGVVISEEA 790 800 810 820 830 840 ATAAVQGAVA MAAPMAGLME KAGKDGPPKS FRELKEAIKI LESLKNMTVE QLLTGSPTSP 850 860 870 880 890 900 TVEPEKPTRE KKFLDDIKKL QENLKKTLDN VAIVEEEKME AVPDVERKED KPEGQSPVKA 910 920 930 940 950 960 EWPSETPVLC QQCGGKPGVT FTSAKGEVFS VLEFAPSNHS FKKIEFQPPE AKKFFSTVRK 970 980 990 1000 1010 1020 EMALLATSLP EGIMVKTFED RMDLFSALIK GPTRTPYEDG LYLFDIQLPN IYPAVPPHFC 1030 1040 1050 1060 1070 1080 YLSQCSGRLN PNLYDNGKVC VSLLGTWIGK GTERWTSKSS LLQVLISIQG LILVNEPYYN 1090 1100 1110 1120 1130 1140 EAGFDSDRGL QEGYENSRCY NEMALIRVVQ SMTQLVRRPP EVFEQEIRQH FSTGGWRLVN 1150 1160 1170 1180 1190 1200 RIESWLETHA LLEKAQALPN GVPKASSSPE PPAVAELSDS GQQEPEDGGP APGEASQGSD 1210 1220 1230 1240 1250 1260 SEGGAQGLAS ASRDHTDQTS ETAPDASVPP SVKPKKRRKS YRSFLPEKSG YPDIGFPLFP 1270 1280 1290 LSKGFIKSIR GVLTQFRAAL LEAGMPECTE DK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Hattori A., Kondo Y., Okumura K., Ohara O. DNA Res. 7:347-355(2000) [PubMed: 11214970] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT SER-1207. Tissue: Brain.
[2]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 270-1292, VARIANT SER-1207. Tissue: Brain and Skin.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 338-1292, VARIANT SER-1207. Tissue: Melanoma.
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 448-1292, VARIANT SER-1207.
[7]	"Identification, tissue expression, and chromosomal position of a novel gene encoding human ubiquitin-conjugating enzyme E2-230k." Yokota T., Nagai H., Harada H., Mine N., Terada Y., Fujiwara H., Yabe A., Miyazaki K., Emi M. Gene 267:95-100(2001) [PubMed: 11311559] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[8]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-896, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[9]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-838; SER-839 AND THR-841, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87; SER-89; SER-399; SER-401; THR-435; SER-441; SER-836 AND THR-838, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87; SER-89; SER-399; SER-401; SER-836; THR-838 AND SER-839, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[12]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-836, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB051521 mRNA. Translation: BAB21825.1. Different initiation. AC090699 Genomic DNA. No translation available. CH471099 Genomic DNA. Translation: EAW89396.1. BC004525 mRNA. Translation: AAH04525.2. BC022237 mRNA. Translation: AAH22237.1. Different initiation. BC025977 mRNA. Translation: AAH25977.1. Different initiation. BC036820 mRNA. Translation: AAH36820.1. Different initiation. BC051868 mRNA. Translation: AAH51868.2. Different initiation. AL832432 mRNA. Translation: CAH10644.1. AK022940 mRNA. Translation: BAB14320.1. Different initiation. AK024657 mRNA. Translation: BAB14948.1. Different initiation. AK025999 mRNA. Translation: BAB15313.1. Different initiation.
IPI	IPI00783378.
RefSeq	NP_071349.3. NM_022066.3.
UniGene	Hs.16130.
3D structure databases
HSSP	HSSP built from PDB template 1ZDN based on UniProtKB Q16763.
ProteinModelPortal	Q9C0C9.
SMR	Q9C0C9. Positions 936-1150.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9C0C9. 19 interactions.
STRING	Q9C0C9.
PTM databases
PhosphoSite	Q9C0C9.
Polymorphism databases
DMDM	209572710.
Proteomic databases
PRIDE	Q9C0C9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000319380; ENSP00000323687; ENSG00000175931.
GeneID	63893.
KEGG	hsa:63893.
Organism-specific databases
CTD	63893.
GeneCards	GC17M074385.
H-InvDB	HIX0020035.
HGNC	HGNC:29554. UBE2O.
neXtProt	NX_Q9C0C9.
PharmGKB	PA142670651.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17746.
GeneTree	ENSGT00590000082815.
HOVERGEN	HBG080116.
InParanoid	Q9C0C9.
OMA	KSGYPDI.
OrthoDB	EOG45DWP7.
PhylomeDB	Q9C0C9.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	Q9C0C9.
Bgee	Q9C0C9.
CleanEx	HS_UBE2O.
Genevestigator	Q9C0C9.
Family and domain databases
InterPro	IPR000608. UBQ-conjugat_E2. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
KO	K10581.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. False negative. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBE2O_HUMAN

Accession

Primary (citable) accession number: Q9C0C9
Secondary accession number(s): A6NDU5 , Q69YP4, Q6PIZ2, Q86UA4, Q8N425, Q8TBN1, Q9BSW1, Q9H6E6, Q9H7E4, Q9H9B2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	October 14, 2008
Last modified:	January 25, 2012
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.