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Protein

E3 ubiquitin-protein ligase TRIM9

Gene

TRIM9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which ubiquitinates itself in cooperation with an E2 enzyme UBE2D2/UBC4 and serves as a targeting signal for proteasomal degradation. May play a role in regulation of neuronal functions and may also participate in the formation or breakdown of abnormal inclusions in neurodegenerative disorders. May act as a regulator of synaptic vesicle exocytosis by controlling the availability of SNAP25 for the SNARE complex formation.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 50RING-typePROSITE-ProRule annotationAdd BLAST41
Zinc fingeri163 – 212B box-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri224 – 266B box-type 2PROSITE-ProRule annotationAdd BLAST43

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • protein homodimerization activity Source: UniProtKB
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM9 (EC:6.3.2.-)
Alternative name(s):
RING finger protein 91
Tripartite motif-containing protein 9
Gene namesi
Name:TRIM9
Synonyms:KIAA0282, RNF91
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:16288. TRIM9.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB
  • cytoskeleton Source: UniProtKB-SubCell
  • dendrite Source: UniProtKB
  • synaptic vesicle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Synapse

Pathology & Biotechi

Organism-specific databases

DisGeNETi114088.
OpenTargetsiENSG00000100505.
PharmGKBiPA38116.

Polymorphism and mutation databases

BioMutaiTRIM9.
DMDMi33516964.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000562081 – 710E3 ubiquitin-protein ligase TRIM9Add BLAST710

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei41PhosphothreonineBy similarity1
Modified residuei44PhosphoserineBy similarity1
Modified residuei46PhosphoserineBy similarity1
Modified residuei49PhosphoserineBy similarity1

Post-translational modificationi

Auto-ubiquitinated. Poly-ubiquitinated in cultured cells, whereas it is monoubiquitinated in vitro.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9C026.
PaxDbiQ9C026.
PeptideAtlasiQ9C026.
PRIDEiQ9C026.

2D gel databases

REPRODUCTION-2DPAGEQ9C026.

PTM databases

iPTMnetiQ9C026.
PhosphoSitePlusiQ9C026.

Expressioni

Tissue specificityi

Brain. Highly expressed in the cerebral cortex (at protein level). Severely decreased in the affected brain areas in Parkinson disease and dementia with Lewy bodies.1 Publication

Gene expression databases

BgeeiENSG00000100505.
CleanExiHS_TRIM9.
GenevisibleiQ9C026. HS.

Interactioni

Subunit structurei

Interacts with SNAP25.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
A8KAD63EBI-720828,EBI-10174974
AP3M1Q9Y2T23EBI-720828,EBI-2371151
BTRCQ9Y2974EBI-720828,EBI-307461
CSNK1DP487303EBI-720828,EBI-751621
EVLQ9UI083EBI-720828,EBI-346653
GFM2Q969S93EBI-720828,EBI-2371750
MUL1Q969V53EBI-720828,EBI-744120
NFKBIDQ8NI383EBI-720828,EBI-10271199
SPG21Q9NZD85EBI-720828,EBI-742688
TSSK3Q96PN83EBI-720828,EBI-3918381
VASPP505523EBI-720828,EBI-748201

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi125280. 44 interactors.
IntActiQ9C026. 31 interactors.
MINTiMINT-1478450.
STRINGi9606.ENSP00000298355.

Structurei

Secondary structure

1710
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi454 – 460Combined sources7
Beta strandi473 – 479Combined sources7
Beta strandi482 – 485Combined sources4
Beta strandi488 – 494Combined sources7
Beta strandi498 – 501Combined sources4
Beta strandi505 – 507Combined sources3
Beta strandi510 – 516Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DB8NMR-A439-534[»]
ProteinModelPortaliQ9C026.
SMRiQ9C026.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9C026.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini374 – 432COSPROSITE-ProRule annotationAdd BLAST59
Domaini440 – 535Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST96
Domaini533 – 702B30.2/SPRYPROSITE-ProRule annotationAdd BLAST170

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili273 – 340Sequence analysisAdd BLAST68

Domaini

The coiled coil domain mediates the interaction with the N-terminal t-SNARE domain of SNAP25.By similarity

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 2 B box-type zinc fingers.PROSITE-ProRule annotation
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 COS domain.PROSITE-ProRule annotation
Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri10 – 50RING-typePROSITE-ProRule annotationAdd BLAST41
Zinc fingeri163 – 212B box-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri224 – 266B box-type 2PROSITE-ProRule annotationAdd BLAST43

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG4367. Eukaryota.
ENOG410XQMV. LUCA.
GeneTreeiENSGT00760000118878.
HOVERGENiHBG062305.
InParanoidiQ9C026.
KOiK10649.
OMAiPIPDFYT.
OrthoDBiEOG091G0PBB.
PhylomeDBiQ9C026.
TreeFamiTF315216.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 1 hit.
3.30.40.10. 2 hits.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003649. Bbox_C.
IPR013320. ConA-like_dom.
IPR017903. COS_domain.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR003877. SPRY_dom.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
SMARTiSM00502. BBC. 1 hit.
SM00336. BBOX. 2 hits.
SM00060. FN3. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS51262. COS. 1 hit.
PS50853. FN3. 1 hit.
PS50119. ZF_BBOX. 2 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9C026-1) [UniParc]FASTAAdd to basket
Also known as: Beta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEMEEELKC PVCGSFYREP IILPCSHNLC QACARNILVQ TPESESPQSH
60 70 80 90 100
RAAGSGVSDY DYLDLDKMSL YSEADSGYGS YGGFASAPTT PCQKSPNGVR
110 120 130 140 150
VFPPAMPPPA THLSPALAPV PRNSCITCPQ CHRSLILDDR GLRGFPKNRV
160 170 180 190 200
LEGVIDRYQQ SKAAALKCQL CEKAPKEATV MCEQCDVFYC DPCRLRCHPP
210 220 230 240 250
RGPLAKHRLV PPAQGRVSRR LSPRKVSTCT DHELENHSMY CVQCKMPVCY
260 270 280 290 300
QCLEEGKHSS HEVKALGAMW KLHKSQLSQA LNGLSDRAKE AKEFLVQLRN
310 320 330 340 350
MVQQIQENSV EFEACLVAQC DALIDALNRR KAQLLARVNK EHEHKLKVVR
360 370 380 390 400
DQISHCTVKL RQTTGLMEYC LEVIKENDPS GFLQISDALI RRVHLTEDQW
410 420 430 440 450
GKGTLTPRMT TDFDLSLDNS PLLQSIHQLD FVQVKASSPV PATPILQLEE
460 470 480 490 500
CCTHNNSATL SWKQPPLSTV PADGYILELD DGNGGQFREV YVGKETMCTV
510 520 530 540 550
DGLHFNSTYN ARVKAFNKTG VSPYSKTLVL QTSEVAWFAF DPGSAHSDII
560 570 580 590 600
LSNDNLTVTC SSYDDRVVLG KTGFSKGIHY WELTVDRYDN HPDPAFGVAR
610 620 630 640 650
MDVMKDVMLG KDDKAWAMYV DNNRSWFMHN NSHTNRTEGG ITKGATIGVL
660 670 680 690 700
LDLNRKNLTF FINDEQQGPI AFDNVEGLFF PAVSLNRNVQ VTLHTGLPVP
710
DFYSSRASIA
Length:710
Mass (Da):79,177
Last modified:June 1, 2001 - v1
Checksum:iAEAB24807C89D0E2
GO
Isoform 4 (identifier: Q9C026-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     436-439: Missing.
     534-534: E → EDTDSEEQTL...QLVDIKKLLA
     692-710: TLHTGLPVPDFYSSRASIA → STLPLRLNSCCWLPVQRLPRAVQSNRREGS

Note: May be due to a competing donor splice site, to exon inclusion and to intron retention.
Show »
Length:802
Mass (Da):89,762
Checksum:i38ABF6696A12C84C
GO
Isoform 5 (identifier: Q9C026-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     535-550: VAWFAFDPGSAHSDII → GKALQQYPSERELRGI
     551-710: Missing.

Note: May be due to intron retention.
Show »
Length:550
Mass (Da):61,346
Checksum:i47ACD9147C1BE651
GO

Sequence cautioni

The sequence AAG53490 differs from that shown. Reason: Frameshift at position 660.Curated
The sequence AAG53492 differs from that shown. Reason: Frameshift at position 655.Curated
The sequence BAA13398 differs from that shown. Reason: Frameshift at position 660.Curated
The sequence BAA13398 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti314A → V in BAB70913 (PubMed:9179496).Curated1
Sequence conflicti384Q → R in BAB70913 (PubMed:9179496).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_016202653L → F.3 PublicationsCorresponds to variant rs2275462dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007922436 – 439Missing in isoform 4. 1 Publication4
Alternative sequenceiVSP_007923534E → EDTDSEEQTLPFPVPSERLP LRRMSPFSSTLNLQPSFPGR SYFDFRSSPHQLSLHSSLQS LNAPGCNFETQSAPYSQLVD IKKLLA in isoform 4. 1 Publication1
Alternative sequenceiVSP_007925535 – 550VAWFA…HSDII → GKALQQYPSERELRGI in isoform 5. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_007926551 – 710Missing in isoform 5. 1 PublicationAdd BLAST160
Alternative sequenceiVSP_007924692 – 710TLHTG…RASIA → STLPLRLNSCCWLPVQRLPR AVQSNRREGS in isoform 4. 1 PublicationAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF220036 mRNA. Translation: AAG53490.1. Frameshift.
AF220037 mRNA. Translation: AAG53491.1.
AF220038 mRNA. Translation: AAG53492.1. Frameshift.
D87458 mRNA. Translation: BAA13398.2. Sequence problems.
AK055388 mRNA. Translation: BAB70913.1.
CH471078 Genomic DNA. Translation: EAW65680.1.
CH471078 Genomic DNA. Translation: EAW65681.1.
CH471078 Genomic DNA. Translation: EAW65682.1.
CH471078 Genomic DNA. Translation: EAW65684.1.
BC013414 mRNA. Translation: AAH13414.1.
BC063872 mRNA. Translation: AAH63872.1.
CCDSiCCDS45105.1. [Q9C026-5]
CCDS9703.1. [Q9C026-1]
RefSeqiNP_055978.4. NM_015163.5. [Q9C026-1]
NP_443210.1. NM_052978.4. [Q9C026-5]
XP_011534691.1. XM_011536389.2. [Q9C026-4]
XP_016876434.1. XM_017020945.1. [Q9C026-1]
UniGeneiHs.654750.

Genome annotation databases

EnsembliENST00000298355; ENSP00000298355; ENSG00000100505. [Q9C026-1]
ENST00000338969; ENSP00000342970; ENSG00000100505. [Q9C026-4]
ENST00000360392; ENSP00000353561; ENSG00000100505. [Q9C026-5]
GeneIDi114088.
KEGGihsa:114088.
UCSCiuc001wyx.5. human. [Q9C026-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF220036 mRNA. Translation: AAG53490.1. Frameshift.
AF220037 mRNA. Translation: AAG53491.1.
AF220038 mRNA. Translation: AAG53492.1. Frameshift.
D87458 mRNA. Translation: BAA13398.2. Sequence problems.
AK055388 mRNA. Translation: BAB70913.1.
CH471078 Genomic DNA. Translation: EAW65680.1.
CH471078 Genomic DNA. Translation: EAW65681.1.
CH471078 Genomic DNA. Translation: EAW65682.1.
CH471078 Genomic DNA. Translation: EAW65684.1.
BC013414 mRNA. Translation: AAH13414.1.
BC063872 mRNA. Translation: AAH63872.1.
CCDSiCCDS45105.1. [Q9C026-5]
CCDS9703.1. [Q9C026-1]
RefSeqiNP_055978.4. NM_015163.5. [Q9C026-1]
NP_443210.1. NM_052978.4. [Q9C026-5]
XP_011534691.1. XM_011536389.2. [Q9C026-4]
XP_016876434.1. XM_017020945.1. [Q9C026-1]
UniGeneiHs.654750.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DB8NMR-A439-534[»]
ProteinModelPortaliQ9C026.
SMRiQ9C026.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125280. 44 interactors.
IntActiQ9C026. 31 interactors.
MINTiMINT-1478450.
STRINGi9606.ENSP00000298355.

PTM databases

iPTMnetiQ9C026.
PhosphoSitePlusiQ9C026.

Polymorphism and mutation databases

BioMutaiTRIM9.
DMDMi33516964.

2D gel databases

REPRODUCTION-2DPAGEQ9C026.

Proteomic databases

MaxQBiQ9C026.
PaxDbiQ9C026.
PeptideAtlasiQ9C026.
PRIDEiQ9C026.

Protocols and materials databases

DNASUi114088.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000298355; ENSP00000298355; ENSG00000100505. [Q9C026-1]
ENST00000338969; ENSP00000342970; ENSG00000100505. [Q9C026-4]
ENST00000360392; ENSP00000353561; ENSG00000100505. [Q9C026-5]
GeneIDi114088.
KEGGihsa:114088.
UCSCiuc001wyx.5. human. [Q9C026-1]

Organism-specific databases

CTDi114088.
DisGeNETi114088.
GeneCardsiTRIM9.
HGNCiHGNC:16288. TRIM9.
MIMi606555. gene.
neXtProtiNX_Q9C026.
OpenTargetsiENSG00000100505.
PharmGKBiPA38116.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4367. Eukaryota.
ENOG410XQMV. LUCA.
GeneTreeiENSGT00760000118878.
HOVERGENiHBG062305.
InParanoidiQ9C026.
KOiK10649.
OMAiPIPDFYT.
OrthoDBiEOG091G0PBB.
PhylomeDBiQ9C026.
TreeFamiTF315216.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiTRIM9. human.
EvolutionaryTraceiQ9C026.
GeneWikiiTRIM9.
GenomeRNAii114088.
PROiQ9C026.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100505.
CleanExiHS_TRIM9.
GenevisibleiQ9C026. HS.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 1 hit.
3.30.40.10. 2 hits.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003649. Bbox_C.
IPR013320. ConA-like_dom.
IPR017903. COS_domain.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR003877. SPRY_dom.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00041. fn3. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
[Graphical view]
SMARTiSM00502. BBC. 1 hit.
SM00336. BBOX. 2 hits.
SM00060. FN3. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS51262. COS. 1 hit.
PS50853. FN3. 1 hit.
PS50119. ZF_BBOX. 2 hits.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRIM9_HUMAN
AccessioniPrimary (citable) accession number: Q9C026
Secondary accession number(s): D3DSB7
, D3DSB8, Q92557, Q96D24, Q96NI4, Q9C025, Q9C027
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 4, 2003
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.