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Protein

Apoptosis inhibitor 5

Gene

API5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.3 Publications

GO - Molecular functioni

  • fibroblast growth factor binding Source: UniProtKB
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of fibroblast apoptotic process Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis inhibitor 5
Short name:
API-5
Alternative name(s):
Antiapoptosis clone 11 protein
Short name:
AAC-11
Cell migration-inducing gene 8 protein
Fibroblast growth factor 2-interacting factor
Short name:
FIF
Protein XAGL
Gene namesi
Name:API5
ORF Names:MIG8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:594. API5.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: Mainly nuclear. Can also be cytoplasmic.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • membrane Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • spliceosomal complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24881.

Polymorphism and mutation databases

BioMutaiAPI5.
DMDMi353526346.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 524523Apoptosis inhibitor 5PRO_0000064634Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei251 – 2511N6-acetyllysine1 Publication
Modified residuei399 – 3991PhosphothreonineCombined sources
Modified residuei462 – 4621PhosphoserineCombined sources
Modified residuei464 – 4641PhosphoserineCombined sources
Modified residuei469 – 4691PhosphoserineBy similarity

Post-translational modificationi

Acetylation at Lys-251 impairs antiapoptotic function.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BZZ5.
MaxQBiQ9BZZ5.
PaxDbiQ9BZZ5.
PeptideAtlasiQ9BZZ5.
PRIDEiQ9BZZ5.

PTM databases

iPTMnetiQ9BZZ5.
PhosphoSiteiQ9BZZ5.
SwissPalmiQ9BZZ5.

Expressioni

Tissue specificityi

Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.5 Publications

Gene expression databases

BgeeiENSG00000166181.
CleanExiHS_API5.
ExpressionAtlasiQ9BZZ5. baseline and differential.
GenevisibleiQ9BZZ5. HS.

Organism-specific databases

HPAiHPA026598.
HPA048801.

Interactioni

Subunit structurei

Monomer. Interacts with FGF2 and ACIN1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ACIN1Q9UKV3-32EBI-1048422,EBI-6976596

GO - Molecular functioni

  • fibroblast growth factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi114109. 38 interactions.
IntActiQ9BZZ5. 6 interactions.
MINTiMINT-3086548.
STRINGi9606.ENSP00000431391.

Structurei

Secondary structure

1
524
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1613Combined sources
Helixi18 – 247Combined sources
Helixi25 – 339Combined sources
Helixi34 – 363Combined sources
Helixi39 – 5214Combined sources
Helixi53 – 553Combined sources
Helixi57 – 593Combined sources
Helixi60 – 7112Combined sources
Helixi76 – 8510Combined sources
Helixi86 – 894Combined sources
Helixi95 – 10511Combined sources
Helixi111 – 12717Combined sources
Helixi129 – 14214Combined sources
Helixi145 – 15814Combined sources
Helixi159 – 1613Combined sources
Turni164 – 1663Combined sources
Helixi169 – 18214Combined sources
Helixi188 – 19912Combined sources
Helixi202 – 2043Combined sources
Helixi207 – 22115Combined sources
Turni222 – 2243Combined sources
Beta strandi229 – 2313Combined sources
Helixi232 – 24514Combined sources
Helixi246 – 2483Combined sources
Helixi256 – 26510Combined sources
Helixi267 – 2693Combined sources
Helixi282 – 29413Combined sources
Helixi303 – 31513Combined sources
Helixi340 – 35415Combined sources
Helixi359 – 3624Combined sources
Turni368 – 3703Combined sources
Helixi371 – 39525Combined sources
Helixi402 – 4043Combined sources
Helixi406 – 42722Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3U0RX-ray2.50A1-498[»]
3V6AX-ray2.60A1-454[»]
ProteinModelPortaliQ9BZZ5.
SMRiQ9BZZ5. Positions 2-446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 360359ARM-like and Heat-like helical repeatsAdd
BLAST
Regioni370 – 39122Leucine-zipperAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi454 – 47522Nuclear localization signal1 PublicationAdd
BLAST

Domaini

Two regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2.

Sequence similaritiesi

Belongs to the API5 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2213. Eukaryota.
ENOG410XSH0. LUCA.
GeneTreeiENSGT00390000010991.
HOGENOMiHOG000239017.
HOVERGENiHBG050542.
InParanoidiQ9BZZ5.
OMAiWHPEDPE.
OrthoDBiEOG091G06NK.
PhylomeDBiQ9BZZ5.
TreeFamiTF324283.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR008383. API5.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
PfamiPF05918. API5. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 4 (identifier: Q9BZZ5-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPTVEELYRN YGILADATEQ VGQHKDAYQV ILDGVKGGTK EKRLAAQFIP
60 70 80 90 100
KFFKHFPELA DSAINAQLDL CEDEDVSIRR QAIKELPQFA TGENLPRVAD
110 120 130 140 150
ILTQLLQTDD SAEFNLVNNA LLSIFKMDAK GTLGGLFSQI LQGEDIVRER
160 170 180 190 200
AIKFLSTKLK TLPDEVLTKE VEELILTESK KVLEDVTGEE FVLFMKILSG
210 220 230 240 250
LKSLQTVSGR QQLVELVAEQ ADLEQTFNPS DPDCVDRLLQ CTRQAVPLFS
260 270 280 290 300
KNVHSTRFVT YFCEQVLPNL GTLTTPVEGL DIQLEVLKLL AEMSSFCGDM
310 320 330 340 350
EKLETNLRKL FDKLLEYMPL PPEEAENGEN AGNEEPKLQF SYVECLLYSF
360 370 380 390 400
HQLGRKLPDF LTAKLNAEKL KDFKIRLQYF ARGLQVYIRQ LRLALQGKTG
410 420 430 440 450
EALKTEENKI KVVALKITNN INVLIKDLFH IPPSYKSTVT LSWKPVQKVE
460 470 480 490 500
IGQKRASEDT TSGSPPKKSS AGPKRDARQI YNPPSGKYSS NLGNFNYEQR
510 520
GAFRGSRGGR GWGTRGNRSR GRLY
Length:524
Mass (Da):59,005
Last modified:October 19, 2011 - v3
Checksum:iA2EA6BE4D8318BB9
GO
Isoform 1 (identifier: Q9BZZ5-1) [UniParc]FASTAAdd to basket
Also known as: FIF-510

The sequence of this isoform differs from the canonical sequence as follows:
     1-72: Missing.
     498-510: EQRGAFRGSRGGR → GERFRLGTRNMRD
     511-524: Missing.

Note: Incomplete sequence.
Show »
Length:438
Mass (Da):49,497
Checksum:i1EEDA88187A989A2
GO
Isoform 2 (identifier: Q9BZZ5-2) [UniParc]FASTAAdd to basket
Also known as: FIF-504

The sequence of this isoform differs from the canonical sequence as follows:
     499-504: QRGAFR → RSLQGK
     505-524: Missing.

Show »
Length:504
Mass (Da):56,770
Checksum:iD4A0D283CDFC4A87
GO
Isoform 3 (identifier: Q9BZZ5-3) [UniParc]FASTAAdd to basket
Also known as: FIF C1

The sequence of this isoform differs from the canonical sequence as follows:
     427-431: DLFHI → AKESQ
     432-510: Missing.

Note: No experimental confirmation available.
Show »
Length:445
Mass (Da):50,310
Checksum:i43B0F4BC4F78FDC3
GO
Isoform 5 (identifier: Q9BZZ5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     24-77: Missing.
     499-504: QRGAFR → RSLQGK
     505-524: Missing.

Note: No experimental confirmation available.
Show »
Length:450
Mass (Da):50,740
Checksum:iD09D2CC020A382CC
GO
Isoform 6 (identifier: Q9BZZ5-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: MPTVEELYRNYGILADATEQVGQ → MKQRWRENGIGK

Note: No experimental confirmation available.
Show »
Length:513
Mass (Da):57,909
Checksum:i11634F148F8009C2
GO

Sequence cautioni

The sequence AAB86528 differs from that shown. Reason: Frameshift at several positions. Curated
The sequence AAB86528 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti371 – 3733KDF → HES in AAB86528 (PubMed:9307294).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti276 – 2761P → S.1 Publication
VAR_021519
Natural varianti300 – 3001M → V.1 Publication
Corresponds to variant rs5743240 [ dbSNP | Ensembl ].
VAR_021520
Natural varianti493 – 4931G → S.1 Publication
Corresponds to variant rs2862934 [ dbSNP | Ensembl ].
VAR_021521

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7272Missing in isoform 1. 1 PublicationVSP_041968Add
BLAST
Alternative sequencei1 – 2323MPTVE…EQVGQ → MKQRWRENGIGK in isoform 6. 1 PublicationVSP_057412Add
BLAST
Alternative sequencei24 – 7754Missing in isoform 5. 1 PublicationVSP_043289Add
BLAST
Alternative sequencei427 – 4315DLFHI → AKESQ in isoform 3. 1 PublicationVSP_039761
Alternative sequencei432 – 51079Missing in isoform 3. 1 PublicationVSP_039762Add
BLAST
Alternative sequencei498 – 51013EQRGA…SRGGR → GERFRLGTRNMRD in isoform 1. 1 PublicationVSP_041969Add
BLAST
Alternative sequencei499 – 5046QRGAFR → RSLQGK in isoform 2 and isoform 5. 6 PublicationsVSP_041970
Alternative sequencei505 – 52420Missing in isoform 2 and isoform 5. 6 PublicationsVSP_041971Add
BLAST
Alternative sequencei511 – 52414Missing in isoform 1. 1 PublicationVSP_041972Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83857 mRNA. Translation: AAB86528.1. Sequence problems.
Y15906 mRNA. Translation: CAA75867.1.
AF229253 mRNA. Translation: AAK00737.1.
AF229254 mRNA. Translation: AAK00738.1.
AY311389 mRNA. Translation: AAQ76714.1.
BT007093 mRNA. Translation: AAP35756.1.
AY265973 Genomic DNA. Translation: AAO89077.1.
AK294724 mRNA. Translation: BAG57871.1.
AK299288 mRNA. Translation: BAG61304.1.
AK304157 mRNA. Translation: BAG65045.1.
AC087276 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68098.1.
CH471064 Genomic DNA. Translation: EAW68099.1.
BC017709 mRNA. Translation: AAH17709.1.
W40304 mRNA. No translation available.
CCDSiCCDS31465.1. [Q9BZZ5-2]
CCDS44572.1. [Q9BZZ5-4]
CCDS44573.1. [Q9BZZ5-5]
RefSeqiNP_001136402.1. NM_001142930.1. [Q9BZZ5-4]
NP_001136403.1. NM_001142931.1. [Q9BZZ5-5]
NP_001230676.1. NM_001243747.1.
NP_006586.1. NM_006595.3. [Q9BZZ5-2]
UniGeneiHs.435771.

Genome annotation databases

EnsembliENST00000378852; ENSP00000368129; ENSG00000166181. [Q9BZZ5-2]
ENST00000420461; ENSP00000402540; ENSG00000166181. [Q9BZZ5-5]
ENST00000455725; ENSP00000399341; ENSG00000166181. [Q9BZZ5-6]
ENST00000531273; ENSP00000431391; ENSG00000166181. [Q9BZZ5-4]
GeneIDi8539.
KEGGihsa:8539.
UCSCiuc001mxf.3. human. [Q9BZZ5-4]
uc010rfg.2. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83857 mRNA. Translation: AAB86528.1. Sequence problems.
Y15906 mRNA. Translation: CAA75867.1.
AF229253 mRNA. Translation: AAK00737.1.
AF229254 mRNA. Translation: AAK00738.1.
AY311389 mRNA. Translation: AAQ76714.1.
BT007093 mRNA. Translation: AAP35756.1.
AY265973 Genomic DNA. Translation: AAO89077.1.
AK294724 mRNA. Translation: BAG57871.1.
AK299288 mRNA. Translation: BAG61304.1.
AK304157 mRNA. Translation: BAG65045.1.
AC087276 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68098.1.
CH471064 Genomic DNA. Translation: EAW68099.1.
BC017709 mRNA. Translation: AAH17709.1.
W40304 mRNA. No translation available.
CCDSiCCDS31465.1. [Q9BZZ5-2]
CCDS44572.1. [Q9BZZ5-4]
CCDS44573.1. [Q9BZZ5-5]
RefSeqiNP_001136402.1. NM_001142930.1. [Q9BZZ5-4]
NP_001136403.1. NM_001142931.1. [Q9BZZ5-5]
NP_001230676.1. NM_001243747.1.
NP_006586.1. NM_006595.3. [Q9BZZ5-2]
UniGeneiHs.435771.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3U0RX-ray2.50A1-498[»]
3V6AX-ray2.60A1-454[»]
ProteinModelPortaliQ9BZZ5.
SMRiQ9BZZ5. Positions 2-446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114109. 38 interactions.
IntActiQ9BZZ5. 6 interactions.
MINTiMINT-3086548.
STRINGi9606.ENSP00000431391.

PTM databases

iPTMnetiQ9BZZ5.
PhosphoSiteiQ9BZZ5.
SwissPalmiQ9BZZ5.

Polymorphism and mutation databases

BioMutaiAPI5.
DMDMi353526346.

Proteomic databases

EPDiQ9BZZ5.
MaxQBiQ9BZZ5.
PaxDbiQ9BZZ5.
PeptideAtlasiQ9BZZ5.
PRIDEiQ9BZZ5.

Protocols and materials databases

DNASUi8539.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000378852; ENSP00000368129; ENSG00000166181. [Q9BZZ5-2]
ENST00000420461; ENSP00000402540; ENSG00000166181. [Q9BZZ5-5]
ENST00000455725; ENSP00000399341; ENSG00000166181. [Q9BZZ5-6]
ENST00000531273; ENSP00000431391; ENSG00000166181. [Q9BZZ5-4]
GeneIDi8539.
KEGGihsa:8539.
UCSCiuc001mxf.3. human. [Q9BZZ5-4]
uc010rfg.2. human.

Organism-specific databases

CTDi8539.
GeneCardsiAPI5.
HGNCiHGNC:594. API5.
HPAiHPA026598.
HPA048801.
MIMi609774. gene.
neXtProtiNX_Q9BZZ5.
PharmGKBiPA24881.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2213. Eukaryota.
ENOG410XSH0. LUCA.
GeneTreeiENSGT00390000010991.
HOGENOMiHOG000239017.
HOVERGENiHBG050542.
InParanoidiQ9BZZ5.
OMAiWHPEDPE.
OrthoDBiEOG091G06NK.
PhylomeDBiQ9BZZ5.
TreeFamiTF324283.

Miscellaneous databases

ChiTaRSiAPI5. human.
GeneWikiiAPI5.
GenomeRNAii8539.
PROiQ9BZZ5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166181.
CleanExiHS_API5.
ExpressionAtlasiQ9BZZ5. baseline and differential.
GenevisibleiQ9BZZ5. HS.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR008383. API5.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
PfamiPF05918. API5. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPI5_HUMAN
AccessioniPrimary (citable) accession number: Q9BZZ5
Secondary accession number(s): B4DGR0
, B4DRJ2, B4E283, D3DR21, O15441, Q9Y4J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: October 19, 2011
Last modified: September 7, 2016
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.