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Protein

Apoptosis inhibitor 5

Gene

API5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.3 Publications

GO - Molecular functioni

  • fibroblast growth factor binding Source: UniProtKB
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of fibroblast apoptotic process Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis inhibitor 5
Short name:
API-5
Alternative name(s):
Antiapoptosis clone 11 protein
Short name:
AAC-11
Cell migration-inducing gene 8 protein
Fibroblast growth factor 2-interacting factor
Short name:
FIF
Protein XAGL
Gene namesi
Name:API5
ORF Names:MIG8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:594. API5.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: Mainly nuclear. Can also be cytoplasmic.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • membrane Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • spliceosomal complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi8539.
OpenTargetsiENSG00000166181.
PharmGKBiPA24881.

Polymorphism and mutation databases

BioMutaiAPI5.
DMDMi353526346.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000646342 – 524Apoptosis inhibitor 5Add BLAST523

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei251N6-acetyllysine1 Publication1
Modified residuei399PhosphothreonineCombined sources1
Modified residuei462PhosphoserineCombined sources1
Modified residuei464PhosphoserineCombined sources1
Modified residuei469PhosphoserineBy similarity1
Modified residuei500Omega-N-methylarginineCombined sources1

Post-translational modificationi

Acetylation at Lys-251 impairs antiapoptotic function.1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9BZZ5.
PaxDbiQ9BZZ5.
PeptideAtlasiQ9BZZ5.
PRIDEiQ9BZZ5.

PTM databases

iPTMnetiQ9BZZ5.
PhosphoSitePlusiQ9BZZ5.
SwissPalmiQ9BZZ5.

Expressioni

Tissue specificityi

Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.5 Publications

Gene expression databases

BgeeiENSG00000166181.
CleanExiHS_API5.
ExpressionAtlasiQ9BZZ5. baseline and differential.
GenevisibleiQ9BZZ5. HS.

Organism-specific databases

HPAiHPA026598.
HPA048801.

Interactioni

Subunit structurei

Monomer. Interacts with FGF2 and ACIN1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ACIN1Q9UKV3-32EBI-1048422,EBI-6976596

GO - Molecular functioni

  • fibroblast growth factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi114109. 39 interactors.
IntActiQ9BZZ5. 7 interactors.
MINTiMINT-3086548.
STRINGi9606.ENSP00000431391.

Structurei

Secondary structure

1524
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 16Combined sources13
Helixi18 – 24Combined sources7
Helixi25 – 33Combined sources9
Helixi34 – 36Combined sources3
Helixi39 – 52Combined sources14
Helixi53 – 55Combined sources3
Helixi57 – 59Combined sources3
Helixi60 – 71Combined sources12
Helixi76 – 85Combined sources10
Helixi86 – 89Combined sources4
Helixi95 – 105Combined sources11
Helixi111 – 127Combined sources17
Helixi129 – 142Combined sources14
Helixi145 – 158Combined sources14
Helixi159 – 161Combined sources3
Turni164 – 166Combined sources3
Helixi169 – 182Combined sources14
Helixi188 – 199Combined sources12
Helixi202 – 204Combined sources3
Helixi207 – 221Combined sources15
Turni222 – 224Combined sources3
Beta strandi229 – 231Combined sources3
Helixi232 – 245Combined sources14
Helixi246 – 248Combined sources3
Helixi256 – 265Combined sources10
Helixi267 – 269Combined sources3
Helixi282 – 294Combined sources13
Helixi303 – 315Combined sources13
Helixi340 – 354Combined sources15
Helixi359 – 362Combined sources4
Turni368 – 370Combined sources3
Helixi371 – 395Combined sources25
Helixi402 – 404Combined sources3
Helixi406 – 427Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3U0RX-ray2.50A1-498[»]
3V6AX-ray2.60A1-454[»]
ProteinModelPortaliQ9BZZ5.
SMRiQ9BZZ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 360ARM-like and Heat-like helical repeatsAdd BLAST359
Regioni370 – 391Leucine-zipperAdd BLAST22

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi454 – 475Nuclear localization signal1 PublicationAdd BLAST22

Domaini

Two regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2.

Sequence similaritiesi

Belongs to the API5 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2213. Eukaryota.
ENOG410XSH0. LUCA.
GeneTreeiENSGT00390000010991.
HOGENOMiHOG000239017.
HOVERGENiHBG050542.
InParanoidiQ9BZZ5.
OMAiWHPEDPE.
OrthoDBiEOG091G06NK.
PhylomeDBiQ9BZZ5.
TreeFamiTF324283.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR008383. API5.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
PfamiPF05918. API5. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 4 (identifier: Q9BZZ5-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPTVEELYRN YGILADATEQ VGQHKDAYQV ILDGVKGGTK EKRLAAQFIP
60 70 80 90 100
KFFKHFPELA DSAINAQLDL CEDEDVSIRR QAIKELPQFA TGENLPRVAD
110 120 130 140 150
ILTQLLQTDD SAEFNLVNNA LLSIFKMDAK GTLGGLFSQI LQGEDIVRER
160 170 180 190 200
AIKFLSTKLK TLPDEVLTKE VEELILTESK KVLEDVTGEE FVLFMKILSG
210 220 230 240 250
LKSLQTVSGR QQLVELVAEQ ADLEQTFNPS DPDCVDRLLQ CTRQAVPLFS
260 270 280 290 300
KNVHSTRFVT YFCEQVLPNL GTLTTPVEGL DIQLEVLKLL AEMSSFCGDM
310 320 330 340 350
EKLETNLRKL FDKLLEYMPL PPEEAENGEN AGNEEPKLQF SYVECLLYSF
360 370 380 390 400
HQLGRKLPDF LTAKLNAEKL KDFKIRLQYF ARGLQVYIRQ LRLALQGKTG
410 420 430 440 450
EALKTEENKI KVVALKITNN INVLIKDLFH IPPSYKSTVT LSWKPVQKVE
460 470 480 490 500
IGQKRASEDT TSGSPPKKSS AGPKRDARQI YNPPSGKYSS NLGNFNYEQR
510 520
GAFRGSRGGR GWGTRGNRSR GRLY
Length:524
Mass (Da):59,005
Last modified:October 19, 2011 - v3
Checksum:iA2EA6BE4D8318BB9
GO
Isoform 1 (identifier: Q9BZZ5-1) [UniParc]FASTAAdd to basket
Also known as: FIF-510

The sequence of this isoform differs from the canonical sequence as follows:
     1-72: Missing.
     498-510: EQRGAFRGSRGGR → GERFRLGTRNMRD
     511-524: Missing.

Note: Incomplete sequence.
Show »
Length:438
Mass (Da):49,497
Checksum:i1EEDA88187A989A2
GO
Isoform 2 (identifier: Q9BZZ5-2) [UniParc]FASTAAdd to basket
Also known as: FIF-504

The sequence of this isoform differs from the canonical sequence as follows:
     499-504: QRGAFR → RSLQGK
     505-524: Missing.

Show »
Length:504
Mass (Da):56,770
Checksum:iD4A0D283CDFC4A87
GO
Isoform 3 (identifier: Q9BZZ5-3) [UniParc]FASTAAdd to basket
Also known as: FIF C1

The sequence of this isoform differs from the canonical sequence as follows:
     427-431: DLFHI → AKESQ
     432-510: Missing.

Note: No experimental confirmation available.
Show »
Length:445
Mass (Da):50,310
Checksum:i43B0F4BC4F78FDC3
GO
Isoform 5 (identifier: Q9BZZ5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     24-77: Missing.
     499-504: QRGAFR → RSLQGK
     505-524: Missing.

Note: No experimental confirmation available.
Show »
Length:450
Mass (Da):50,740
Checksum:iD09D2CC020A382CC
GO
Isoform 6 (identifier: Q9BZZ5-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: MPTVEELYRNYGILADATEQVGQ → MKQRWRENGIGK

Note: No experimental confirmation available.
Show »
Length:513
Mass (Da):57,909
Checksum:i11634F148F8009C2
GO

Sequence cautioni

The sequence AAB86528 differs from that shown. Reason: Frameshift at several positions.Curated
The sequence AAB86528 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti371 – 373KDF → HES in AAB86528 (PubMed:9307294).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_021519276P → S.1 Publication1
Natural variantiVAR_021520300M → V.1 PublicationCorresponds to variant rs5743240dbSNPEnsembl.1
Natural variantiVAR_021521493G → S.1 PublicationCorresponds to variant rs2862934dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0419681 – 72Missing in isoform 1. 1 PublicationAdd BLAST72
Alternative sequenceiVSP_0574121 – 23MPTVE…EQVGQ → MKQRWRENGIGK in isoform 6. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_04328924 – 77Missing in isoform 5. 1 PublicationAdd BLAST54
Alternative sequenceiVSP_039761427 – 431DLFHI → AKESQ in isoform 3. 1 Publication5
Alternative sequenceiVSP_039762432 – 510Missing in isoform 3. 1 PublicationAdd BLAST79
Alternative sequenceiVSP_041969498 – 510EQRGA…SRGGR → GERFRLGTRNMRD in isoform 1. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_041970499 – 504QRGAFR → RSLQGK in isoform 2 and isoform 5. 6 Publications6
Alternative sequenceiVSP_041971505 – 524Missing in isoform 2 and isoform 5. 6 PublicationsAdd BLAST20
Alternative sequenceiVSP_041972511 – 524Missing in isoform 1. 1 PublicationAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83857 mRNA. Translation: AAB86528.1. Sequence problems.
Y15906 mRNA. Translation: CAA75867.1.
AF229253 mRNA. Translation: AAK00737.1.
AF229254 mRNA. Translation: AAK00738.1.
AY311389 mRNA. Translation: AAQ76714.1.
BT007093 mRNA. Translation: AAP35756.1.
AY265973 Genomic DNA. Translation: AAO89077.1.
AK294724 mRNA. Translation: BAG57871.1.
AK299288 mRNA. Translation: BAG61304.1.
AK304157 mRNA. Translation: BAG65045.1.
AC087276 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68098.1.
CH471064 Genomic DNA. Translation: EAW68099.1.
BC017709 mRNA. Translation: AAH17709.1.
W40304 mRNA. No translation available.
CCDSiCCDS31465.1. [Q9BZZ5-2]
CCDS44572.1. [Q9BZZ5-4]
CCDS44573.1. [Q9BZZ5-5]
RefSeqiNP_001136402.1. NM_001142930.1. [Q9BZZ5-4]
NP_001136403.1. NM_001142931.1. [Q9BZZ5-5]
NP_001230676.1. NM_001243747.1.
NP_006586.1. NM_006595.3. [Q9BZZ5-2]
UniGeneiHs.435771.

Genome annotation databases

EnsembliENST00000378852; ENSP00000368129; ENSG00000166181. [Q9BZZ5-2]
ENST00000420461; ENSP00000402540; ENSG00000166181. [Q9BZZ5-5]
ENST00000455725; ENSP00000399341; ENSG00000166181. [Q9BZZ5-6]
ENST00000531273; ENSP00000431391; ENSG00000166181. [Q9BZZ5-4]
GeneIDi8539.
KEGGihsa:8539.
UCSCiuc001mxf.3. human. [Q9BZZ5-4]
uc010rfg.2. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83857 mRNA. Translation: AAB86528.1. Sequence problems.
Y15906 mRNA. Translation: CAA75867.1.
AF229253 mRNA. Translation: AAK00737.1.
AF229254 mRNA. Translation: AAK00738.1.
AY311389 mRNA. Translation: AAQ76714.1.
BT007093 mRNA. Translation: AAP35756.1.
AY265973 Genomic DNA. Translation: AAO89077.1.
AK294724 mRNA. Translation: BAG57871.1.
AK299288 mRNA. Translation: BAG61304.1.
AK304157 mRNA. Translation: BAG65045.1.
AC087276 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68098.1.
CH471064 Genomic DNA. Translation: EAW68099.1.
BC017709 mRNA. Translation: AAH17709.1.
W40304 mRNA. No translation available.
CCDSiCCDS31465.1. [Q9BZZ5-2]
CCDS44572.1. [Q9BZZ5-4]
CCDS44573.1. [Q9BZZ5-5]
RefSeqiNP_001136402.1. NM_001142930.1. [Q9BZZ5-4]
NP_001136403.1. NM_001142931.1. [Q9BZZ5-5]
NP_001230676.1. NM_001243747.1.
NP_006586.1. NM_006595.3. [Q9BZZ5-2]
UniGeneiHs.435771.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3U0RX-ray2.50A1-498[»]
3V6AX-ray2.60A1-454[»]
ProteinModelPortaliQ9BZZ5.
SMRiQ9BZZ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114109. 39 interactors.
IntActiQ9BZZ5. 7 interactors.
MINTiMINT-3086548.
STRINGi9606.ENSP00000431391.

PTM databases

iPTMnetiQ9BZZ5.
PhosphoSitePlusiQ9BZZ5.
SwissPalmiQ9BZZ5.

Polymorphism and mutation databases

BioMutaiAPI5.
DMDMi353526346.

Proteomic databases

EPDiQ9BZZ5.
PaxDbiQ9BZZ5.
PeptideAtlasiQ9BZZ5.
PRIDEiQ9BZZ5.

Protocols and materials databases

DNASUi8539.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000378852; ENSP00000368129; ENSG00000166181. [Q9BZZ5-2]
ENST00000420461; ENSP00000402540; ENSG00000166181. [Q9BZZ5-5]
ENST00000455725; ENSP00000399341; ENSG00000166181. [Q9BZZ5-6]
ENST00000531273; ENSP00000431391; ENSG00000166181. [Q9BZZ5-4]
GeneIDi8539.
KEGGihsa:8539.
UCSCiuc001mxf.3. human. [Q9BZZ5-4]
uc010rfg.2. human.

Organism-specific databases

CTDi8539.
DisGeNETi8539.
GeneCardsiAPI5.
HGNCiHGNC:594. API5.
HPAiHPA026598.
HPA048801.
MIMi609774. gene.
neXtProtiNX_Q9BZZ5.
OpenTargetsiENSG00000166181.
PharmGKBiPA24881.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2213. Eukaryota.
ENOG410XSH0. LUCA.
GeneTreeiENSGT00390000010991.
HOGENOMiHOG000239017.
HOVERGENiHBG050542.
InParanoidiQ9BZZ5.
OMAiWHPEDPE.
OrthoDBiEOG091G06NK.
PhylomeDBiQ9BZZ5.
TreeFamiTF324283.

Miscellaneous databases

ChiTaRSiAPI5. human.
GeneWikiiAPI5.
GenomeRNAii8539.
PROiQ9BZZ5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166181.
CleanExiHS_API5.
ExpressionAtlasiQ9BZZ5. baseline and differential.
GenevisibleiQ9BZZ5. HS.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR008383. API5.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
PfamiPF05918. API5. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPI5_HUMAN
AccessioniPrimary (citable) accession number: Q9BZZ5
Secondary accession number(s): B4DGR0
, B4DRJ2, B4E283, D3DR21, O15441, Q9Y4J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: October 19, 2011
Last modified: November 30, 2016
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.