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Protein

Uridine-cytidine kinase 2

Gene

UCK2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine.

Catalytic activityi

ATP + uridine = ADP + UMP.
ATP + cytidine = ADP + CMP.

Pathwayi: CTP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes CTP from cytidine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uridine-cytidine kinase 2 (UCK2), Uridine kinase (UCK1), Uridine kinase, Uridine-cytidine kinase 1 (UCK1), Uridine-cytidine kinase-like 1 (UCKL1), Uridine kinase (UCK2)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway CTP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from cytidine, the pathway CTP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uridine.
Proteins known to be involved in this subpathway in this organism are:
  1. Uridine-cytidine kinase 2 (UCK2), Uridine kinase (UCK1), Uridine kinase, Uridine-cytidine kinase 1 (UCK1), Uridine-cytidine kinase-like 1 (UCKL1), Uridine kinase (UCK2)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uridine, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84Substrate1
Binding sitei112Substrate1
Binding sitei117Substrate1
Binding sitei166Substrate1
Binding sitei176Substrate1
Binding sitei184Substrate1
Binding sitei213ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 35ATP9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS07003-MONOMER.
ZFISH:HS07003-MONOMER.
BRENDAi2.7.1.48. 2681.
ReactomeiR-HSA-73614. Pyrimidine salvage reactions.
SABIO-RKQ9BZX2.
UniPathwayiUPA00574; UER00637.
UPA00579; UER00640.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridine-cytidine kinase 2 (EC:2.7.1.48)
Short name:
UCK 2
Alternative name(s):
Cytidine monophosphokinase 2
Testis-specific protein TSA903
Uridine monophosphokinase 2
Gene namesi
Name:UCK2
Synonyms:UMPK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:12562. UCK2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi7371.
OpenTargetsiENSG00000143179.
PharmGKBiPA362.

Chemistry databases

ChEMBLiCHEMBL2469.

Polymorphism and mutation databases

BioMutaiUCK2.
DMDMi20455356.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001644552 – 261Uridine-cytidine kinase 2Add BLAST260

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei254PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BZX2.
MaxQBiQ9BZX2.
PaxDbiQ9BZX2.
PeptideAtlasiQ9BZX2.
PRIDEiQ9BZX2.

PTM databases

iPTMnetiQ9BZX2.
PhosphoSitePlusiQ9BZX2.

Expressioni

Tissue specificityi

According to PubMed:8812458; testis-specific. According to PubMed:11306702, placenta-specific.1 Publication

Gene expression databases

BgeeiENSG00000143179.
CleanExiHS_UCK2.
ExpressionAtlasiQ9BZX2. baseline and differential.
GenevisibleiQ9BZX2. HS.

Organism-specific databases

HPAiHPA051286.

Interactioni

Subunit structurei

Homotetramer.2 Publications

Protein-protein interaction databases

BioGridi113217. 10 interactors.
IntActiQ9BZX2. 1 interactor.
STRINGi9606.ENSP00000356853.

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi21 – 26Combined sources6
Helixi33 – 43Combined sources11
Helixi46 – 48Combined sources3
Helixi51 – 53Combined sources3
Beta strandi55 – 60Combined sources6
Helixi61 – 64Combined sources4
Helixi70 – 77Combined sources8
Helixi86 – 88Combined sources3
Helixi91 – 102Combined sources12
Beta strandi107 – 113Combined sources7
Turni114 – 117Combined sources4
Beta strandi118 – 126Combined sources9
Beta strandi130 – 135Combined sources6
Turni137 – 140Combined sources4
Helixi143 – 148Combined sources6
Beta strandi150 – 156Combined sources7
Helixi159 – 173Combined sources15
Helixi178 – 187Combined sources10
Helixi189 – 196Combined sources8
Helixi198 – 203Combined sources6
Beta strandi205 – 209Combined sources5
Helixi211 – 213Combined sources3
Helixi215 – 230Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UDWX-ray2.60A/B1-250[»]
1UEIX-ray2.60A/B1-250[»]
1UEJX-ray2.61A/B1-250[»]
1UFQX-ray2.50A/B/C/D1-250[»]
1UJ2X-ray1.80A/B1-250[»]
1XRJX-ray2.00A/B1-261[»]
ProteinModelPortaliQ9BZX2.
SMRiQ9BZX2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BZX2.

Family & Domainsi

Sequence similaritiesi

Belongs to the uridine kinase family.Curated

Phylogenomic databases

eggNOGiENOG410IMEH. Eukaryota.
COG0572. LUCA.
GeneTreeiENSGT00390000015696.
HOGENOMiHOG000262756.
HOVERGENiHBG023339.
InParanoidiQ9BZX2.
KOiK00876.
OMAiEHTRTTE.
OrthoDBiEOG091G07OR.
PhylomeDBiQ9BZX2.
TreeFamiTF316686.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR006083. PRK/URK.
IPR029925. UCK-2.
IPR000764. Uridine_kinase-like.
[Graphical view]
PANTHERiPTHR10285:SF28. PTHR10285:SF28. 1 hit.
PfamiPF00485. PRK. 1 hit.
[Graphical view]
PRINTSiPR00988. URIDINKINASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00235. udk. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BZX2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGDSEQTLQ NHQQPNGGEP FLIGVSGGTA SGKSSVCAKI VQLLGQNEVD
60 70 80 90 100
YRQKQVVILS QDSFYRVLTS EQKAKALKGQ FNFDHPDAFD NELILKTLKE
110 120 130 140 150
ITEGKTVQIP VYDFVSHSRK EETVTVYPAD VVLFEGILAF YSQEVRDLFQ
160 170 180 190 200
MKLFVDTDAD TRLSRRVLRD ISERGRDLEQ ILSQYITFVK PAFEEFCLPT
210 220 230 240 250
KKYADVIIPR GADNLVAINL IVQHIQDILN GGPSKRQTNG CLNGYTPSRK
260
RQASESSSRP H
Length:261
Mass (Da):29,299
Last modified:June 1, 2001 - v1
Checksum:i71791346F091EBFD
GO
Isoform 2 (identifier: Q9BZX2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-150: Missing.

Show »
Length:111
Mass (Da):12,587
Checksum:iE6688B1B86F432A9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti202K → Q in BAA11349 (PubMed:8812458).Curated1
Sequence conflicti222V → E in BAA11349 (PubMed:8812458).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0142621 – 150Missing in isoform 2. 3 PublicationsAdd BLAST150

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78335 mRNA. Translation: BAA11349.1.
AF236637 mRNA. Translation: AAK14053.1.
BT006860 mRNA. Translation: AAP35506.1.
CR456857 mRNA. Translation: CAG33138.1.
AL451074, AL358115 Genomic DNA. Translation: CAH74066.1.
AL358115, AL451074 Genomic DNA. Translation: CAI15121.1.
BC002906 mRNA. Translation: AAH02906.2.
AB062451 mRNA. Translation: BAB56162.1.
CCDSiCCDS1252.1. [Q9BZX2-1]
RefSeqiNP_036606.2. NM_012474.4. [Q9BZX2-1]
UniGeneiHs.458360.

Genome annotation databases

EnsembliENST00000367879; ENSP00000356853; ENSG00000143179. [Q9BZX2-1]
ENST00000469256; ENSP00000476692; ENSG00000143179. [Q9BZX2-2]
ENST00000470820; ENSP00000476327; ENSG00000143179. [Q9BZX2-2]
GeneIDi7371.
KEGGihsa:7371.
UCSCiuc021pec.2. human. [Q9BZX2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78335 mRNA. Translation: BAA11349.1.
AF236637 mRNA. Translation: AAK14053.1.
BT006860 mRNA. Translation: AAP35506.1.
CR456857 mRNA. Translation: CAG33138.1.
AL451074, AL358115 Genomic DNA. Translation: CAH74066.1.
AL358115, AL451074 Genomic DNA. Translation: CAI15121.1.
BC002906 mRNA. Translation: AAH02906.2.
AB062451 mRNA. Translation: BAB56162.1.
CCDSiCCDS1252.1. [Q9BZX2-1]
RefSeqiNP_036606.2. NM_012474.4. [Q9BZX2-1]
UniGeneiHs.458360.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UDWX-ray2.60A/B1-250[»]
1UEIX-ray2.60A/B1-250[»]
1UEJX-ray2.61A/B1-250[»]
1UFQX-ray2.50A/B/C/D1-250[»]
1UJ2X-ray1.80A/B1-250[»]
1XRJX-ray2.00A/B1-261[»]
ProteinModelPortaliQ9BZX2.
SMRiQ9BZX2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113217. 10 interactors.
IntActiQ9BZX2. 1 interactor.
STRINGi9606.ENSP00000356853.

Chemistry databases

ChEMBLiCHEMBL2469.

PTM databases

iPTMnetiQ9BZX2.
PhosphoSitePlusiQ9BZX2.

Polymorphism and mutation databases

BioMutaiUCK2.
DMDMi20455356.

Proteomic databases

EPDiQ9BZX2.
MaxQBiQ9BZX2.
PaxDbiQ9BZX2.
PeptideAtlasiQ9BZX2.
PRIDEiQ9BZX2.

Protocols and materials databases

DNASUi7371.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367879; ENSP00000356853; ENSG00000143179. [Q9BZX2-1]
ENST00000469256; ENSP00000476692; ENSG00000143179. [Q9BZX2-2]
ENST00000470820; ENSP00000476327; ENSG00000143179. [Q9BZX2-2]
GeneIDi7371.
KEGGihsa:7371.
UCSCiuc021pec.2. human. [Q9BZX2-1]

Organism-specific databases

CTDi7371.
DisGeNETi7371.
GeneCardsiUCK2.
HGNCiHGNC:12562. UCK2.
HPAiHPA051286.
MIMi609329. gene.
neXtProtiNX_Q9BZX2.
OpenTargetsiENSG00000143179.
PharmGKBiPA362.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IMEH. Eukaryota.
COG0572. LUCA.
GeneTreeiENSGT00390000015696.
HOGENOMiHOG000262756.
HOVERGENiHBG023339.
InParanoidiQ9BZX2.
KOiK00876.
OMAiEHTRTTE.
OrthoDBiEOG091G07OR.
PhylomeDBiQ9BZX2.
TreeFamiTF316686.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00637.
UPA00579; UER00640.
BioCyciMetaCyc:HS07003-MONOMER.
ZFISH:HS07003-MONOMER.
BRENDAi2.7.1.48. 2681.
ReactomeiR-HSA-73614. Pyrimidine salvage reactions.
SABIO-RKQ9BZX2.

Miscellaneous databases

ChiTaRSiUCK2. human.
EvolutionaryTraceiQ9BZX2.
GeneWikiiUCK2.
GenomeRNAii7371.
PROiQ9BZX2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000143179.
CleanExiHS_UCK2.
ExpressionAtlasiQ9BZX2. baseline and differential.
GenevisibleiQ9BZX2. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR006083. PRK/URK.
IPR029925. UCK-2.
IPR000764. Uridine_kinase-like.
[Graphical view]
PANTHERiPTHR10285:SF28. PTHR10285:SF28. 1 hit.
PfamiPF00485. PRK. 1 hit.
[Graphical view]
PRINTSiPR00988. URIDINKINASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00235. udk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUCK2_HUMAN
AccessioniPrimary (citable) accession number: Q9BZX2
Secondary accession number(s): Q5VV91
, Q7KZV3, Q92528, Q96KG5, Q9BU42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.