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Protein

Probable E3 ubiquitin-protein ligase TRIM8

Gene

TRIM8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable E3 ubiquitin-protein ligase which may promote proteasomal degradation of SOCS1.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri15 – 56RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri92 – 132B box-type 1Add BLAST41
Zinc fingeri140 – 182B box-type 2Add BLAST43

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • protein homodimerization activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000171206-MONOMER.
ReactomeiR-HSA-877300. Interferon gamma signaling.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable E3 ubiquitin-protein ligase TRIM8 (EC:6.3.2.-)
Alternative name(s):
Glioblastoma-expressed RING finger protein
RING finger protein 27
Tripartite motif-containing protein 8
Gene namesi
Name:TRIM8
Synonyms:GERP, RNF27
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:15579. TRIM8.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • intracellular membrane-bounded organelle Source: HPA
  • nucleoplasm Source: HPA
  • PML body Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi81603.
OpenTargetsiENSG00000171206.
PharmGKBiPA37983.

Polymorphism and mutation databases

BioMutaiTRIM8.
DMDMi18202744.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000562061 – 551Probable E3 ubiquitin-protein ligase TRIM8Add BLAST551

Proteomic databases

EPDiQ9BZR9.
MaxQBiQ9BZR9.
PaxDbiQ9BZR9.
PeptideAtlasiQ9BZR9.
PRIDEiQ9BZR9.

PTM databases

iPTMnetiQ9BZR9.
PhosphoSitePlusiQ9BZR9.

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

BgeeiENSG00000171206.
CleanExiHS_TRIM8.
ExpressionAtlasiQ9BZR9. baseline and differential.
GenevisibleiQ9BZR9. HS.

Organism-specific databases

HPAiHPA023560.
HPA023561.

Interactioni

Subunit structurei

Homodimer. Interacts with SOCS1 (via) SH2 domain and SOCS box (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-2340370,EBI-2340370
UBE2D1P516683EBI-2340370,EBI-743540
UBE2D4Q9Y2X83EBI-2340370,EBI-745527
UBE2L6O149333EBI-2340370,EBI-2129974

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi123541. 54 interactors.
IntActiQ9BZR9. 59 interactors.
STRINGi9606.ENSP00000302120.

Structurei

3D structure databases

ProteinModelPortaliQ9BZR9.
SMRiQ9BZR9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili181 – 249Sequence analysisAdd BLAST69

Domaini

The coiled coil domain is required for homodimerization.
The region immediately C-terminal to the RING motif is sufficient to mediate the interaction with SOCS1.By similarity

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 2 B box-type zinc fingers.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri15 – 56RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri92 – 132B box-type 1Add BLAST41
Zinc fingeri140 – 182B box-type 2Add BLAST43

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITEZ. Eukaryota.
ENOG410ZTPN. LUCA.
GeneTreeiENSGT00850000132354.
HOGENOMiHOG000082538.
HOVERGENiHBG056254.
InParanoidiQ9BZR9.
KOiK12001.
OMAiEDINFMK.
OrthoDBiEOG091G059M.
PhylomeDBiQ9BZR9.
TreeFamiTF333491.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BZR9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAENWKNCFE EELICPICLH VFVEPVQLPC KHNFCRGCIG EAWAKDSGLV
60 70 80 90 100
RCPECNQAYN QKPGLEKNLK LTNIVEKFNA LHVEKPPAAL HCVFCRRGPP
110 120 130 140 150
LPAQKVCLRC EAPCCQSHVQ THLQQPSTAR GHLLVEADDV RAWSCPQHNA
160 170 180 190 200
YRLYHCEAEQ VAVCQYCCYY SGAHQGHSVC DVEIRRNEIR KMLMKQQDRL
210 220 230 240 250
EEREQDIEDQ LYKLESDKRL VEEKVNQLKE EVRLQYEKLH QLLDEDLRQT
260 270 280 290 300
VEVLDKAQAK FCSENAAQAL HLGERMQEAK KLLGSLQLLF DKTEDVSFMK
310 320 330 340 350
NTKSVKILMD RTQTCTSSSL SPTKIGHLNS KLFLNEVAKK EKQLRKMLEG
360 370 380 390 400
PFSTPVPFLQ SVPLYPCGVS SSGAEKRKHS TAFPEASFLE TSSGPVGGQY
410 420 430 440 450
GAAGTASGEG QSGQPLGPCS STQHLVALPG GAQPVHSSPV FPPSQYPNGS
460 470 480 490 500
AAQQPMLPQY GGRKILVCSV DNCYCSSVAN HGGHQPYPRS GHFPWTVPSQ
510 520 530 540 550
EYSHPLPPTP SVPQSLPSLA VRDWLDASQQ PGHQDFYRVY GQPSTKHYVT

S
Length:551
Mass (Da):61,489
Last modified:September 26, 2001 - v2
Checksum:i1FEF89029EB9BACB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti174H → R in AAG53087 (PubMed:11118312).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF281046 mRNA. Translation: AAG53087.1.
AF220034 mRNA. Translation: AAG53488.1.
AL391121 Genomic DNA. No translation available.
CH471066 Genomic DNA. Translation: EAW49680.1.
BC021925 mRNA. Translation: AAH21925.1.
CCDSiCCDS31274.1.
PIRiJC7562.
RefSeqiNP_112174.2. NM_030912.2.
UniGeneiHs.336810.

Genome annotation databases

EnsembliENST00000302424; ENSP00000302120; ENSG00000171206.
GeneIDi81603.
KEGGihsa:81603.
UCSCiuc001kvz.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF281046 mRNA. Translation: AAG53087.1.
AF220034 mRNA. Translation: AAG53488.1.
AL391121 Genomic DNA. No translation available.
CH471066 Genomic DNA. Translation: EAW49680.1.
BC021925 mRNA. Translation: AAH21925.1.
CCDSiCCDS31274.1.
PIRiJC7562.
RefSeqiNP_112174.2. NM_030912.2.
UniGeneiHs.336810.

3D structure databases

ProteinModelPortaliQ9BZR9.
SMRiQ9BZR9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123541. 54 interactors.
IntActiQ9BZR9. 59 interactors.
STRINGi9606.ENSP00000302120.

PTM databases

iPTMnetiQ9BZR9.
PhosphoSitePlusiQ9BZR9.

Polymorphism and mutation databases

BioMutaiTRIM8.
DMDMi18202744.

Proteomic databases

EPDiQ9BZR9.
MaxQBiQ9BZR9.
PaxDbiQ9BZR9.
PeptideAtlasiQ9BZR9.
PRIDEiQ9BZR9.

Protocols and materials databases

DNASUi81603.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302424; ENSP00000302120; ENSG00000171206.
GeneIDi81603.
KEGGihsa:81603.
UCSCiuc001kvz.3. human.

Organism-specific databases

CTDi81603.
DisGeNETi81603.
GeneCardsiTRIM8.
HGNCiHGNC:15579. TRIM8.
HPAiHPA023560.
HPA023561.
MIMi606125. gene.
neXtProtiNX_Q9BZR9.
OpenTargetsiENSG00000171206.
PharmGKBiPA37983.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ITEZ. Eukaryota.
ENOG410ZTPN. LUCA.
GeneTreeiENSGT00850000132354.
HOGENOMiHOG000082538.
HOVERGENiHBG056254.
InParanoidiQ9BZR9.
KOiK12001.
OMAiEDINFMK.
OrthoDBiEOG091G059M.
PhylomeDBiQ9BZR9.
TreeFamiTF333491.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:ENSG00000171206-MONOMER.
ReactomeiR-HSA-877300. Interferon gamma signaling.

Miscellaneous databases

ChiTaRSiTRIM8. human.
GenomeRNAii81603.
PROiQ9BZR9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000171206.
CleanExiHS_TRIM8.
ExpressionAtlasiQ9BZR9. baseline and differential.
GenevisibleiQ9BZR9. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRIM8_HUMAN
AccessioniPrimary (citable) accession number: Q9BZR9
Secondary accession number(s): A6NI31, Q9C028
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: November 30, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.