Q9BZR8 (B2L14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Apoptosis facilitator Bcl-2-like protein 14
Short name=Bcl2-L-14

Alternative name(s):
Apoptosis regulator Bcl-G

Gene names

Name:	BCL2L14
Synonyms:	BCLG

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in apoptosis.
Subcellular location	Cytoplasm Ref.1. Isoform 1: Cytoplasm › cytosol. Note: Diffusely distributed throughout the cytosol. Ref.1 Isoform 2: Endomembrane system. Note: Predominantly localized to cytosolic organelles. Ref.1
Tissue specificity	Isoform 1 is widely expressed. Isoform 2 is testis-specific. Ref.1
Post-translational modification	Phosphorylated by MELK, leading to inhibit its pro-apoptotic function. Ref.6 Ref.7
Sequence similarities	Belongs to the Bcl-2 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from direct assay Ref.1Ref.6. Source: UniProtKB regulation of apoptotic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay Ref.1. Source: UniProtKB endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell intracellular organelle Inferred from direct assay Ref.1. Source: UniProtKB membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein kinase binding Inferred from physical interaction Ref.6. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MELK	Q14680	4	EBI-1385773,EBI-1046702

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BZR8-1)

Also known as: Bcl-Gl; Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BZR8-2)

Also known as: Bcl-Gs; Short;

The sequence of this isoform differs from the canonical sequence as follows:
     227-252: LKKDKALMGHFQDGLSYSVFKTITDQ → DTAFIPIPLVDTSIQGFPQDGLMACI
     253-327: Missing.

Isoform 3 (identifier: Q9BZR8-3)

Also known as: Median;

The sequence of this isoform differs from the canonical sequence as follows:
     227-276: LKKDKALMGH...AQGFKAALVI → EKEWEESAKT...LTPPGFSEHN
     277-327: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Apoptosis facilitator Bcl-2-like protein 14		PRO_0000143075
Regions
	Motif	212 – 226	15	BH3
	Motif	308 – 315	8	BH2
Amino acid modifications
	Modified residue	152	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	227 – 276	50	LKKDK…AALVI → EKEWEESAKTKIKMALLWRS SGGGPRVLSIPEAFMKNSFT LTPPGFSEHN in isoform 3.		VSP_012241
	Alternative sequence	227 – 252	26	LKKDK…TITDQ → DTAFIPIPLVDTSIQGFPQD GLMACI in isoform 2.		VSP_012239
	Alternative sequence	253 – 327	75	Missing in isoform 2.		VSP_012240
	Alternative sequence	277 – 327	51	Missing in isoform 3.		VSP_012242

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Bcl-Gl) (Long) [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 155D43BA91BFB3EA	FASTA	327	36,598
	10 20 30 40 50 60 MCSTSGCDLE EIPLDDDDLN TIEFKILAYY TRHHVFKSTP ALFSPKLLRT RSLSQRGLGN 70 80 90 100 110 120 CSANESWTEV SWPCRNSQSS EKAINLGKKK SSWKAFFGVV EKEDSQSTPA KVSAQGQRTL 130 140 150 160 170 180 EYQDSHSQQW SRCLSNVEQC LEHEAVDPKV ISIANRVAEI VYSWPPPQAT QAGGFKSKEI 190 200 210 220 230 240 FVTEGLSFQL QGHVPVASSS KKDEEEQILA KIVELLKYSG DQLERKLKKD KALMGHFQDG 250 260 270 280 290 300 LSYSVFKTIT DQVLMGVDPR GESEVKAQGF KAALVIDVTA KLTAIDNHPM NRVLGFGTKY 310 320 LKENFSPWIQ QHGGWEKILG ISHEEVD
	Isoform 2 (Bcl-Gs) (Short) [UniParc]. Checksum: 87D2E5123EFCB9E4	FASTA	252	28,090
	10 20 30 40 50 60 MCSTSGCDLE EIPLDDDDLN TIEFKILAYY TRHHVFKSTP ALFSPKLLRT RSLSQRGLGN 70 80 90 100 110 120 CSANESWTEV SWPCRNSQSS EKAINLGKKK SSWKAFFGVV EKEDSQSTPA KVSAQGQRTL 130 140 150 160 170 180 EYQDSHSQQW SRCLSNVEQC LEHEAVDPKV ISIANRVAEI VYSWPPPQAT QAGGFKSKEI 190 200 210 220 230 240 FVTEGLSFQL QGHVPVASSS KKDEEEQILA KIVELLKYSG DQLERKDTAF IPIPLVDTSI 250 QGFPQDGLMA CI
	Isoform 3 (Median) [UniParc]. Checksum: 81559A7190F5598E	FASTA	276	30,948
	10 20 30 40 50 60 MCSTSGCDLE EIPLDDDDLN TIEFKILAYY TRHHVFKSTP ALFSPKLLRT RSLSQRGLGN 70 80 90 100 110 120 CSANESWTEV SWPCRNSQSS EKAINLGKKK SSWKAFFGVV EKEDSQSTPA KVSAQGQRTL 130 140 150 160 170 180 EYQDSHSQQW SRCLSNVEQC LEHEAVDPKV ISIANRVAEI VYSWPPPQAT QAGGFKSKEI 190 200 210 220 230 240 FVTEGLSFQL QGHVPVASSS KKDEEEQILA KIVELLKYSG DQLERKEKEW EESAKTKIKM 250 260 270 ALLWRSSGGG PRVLSIPEAF MKNSFTLTPP GFSEHN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bcl-G, a novel pro-apoptotic member of the Bcl-2 family." Guo B., Godzik A., Reed J.C. J. Biol. Chem. 276:2780-2785(2001) [PubMed: 11054413] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[2]	"Mutational and expression analysis of the chromosome 12p candidate tumor suppressor genes in pre-B acute lymphoblastic leukemia." Montpetit A., Larose J., Boily G., Langlois S., Trudel N., Sinnett D. Leukemia 18:1499-1504(2004) [PubMed: 15284860] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Trachea.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Pancreas.
[6]	"Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family." Lin M.L., Park J.H., Nishidate T., Nakamura Y., Katagiri T. Breast Cancer Res. 9:R17-R17(2007) [PubMed: 17280616] [Abstract] Cited for: PHOSPHORYLATION BY MELK.
[7]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-152, MASS SPECTROMETRY. Tissue: Platelet.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF281254 mRNA. Translation: AAG59793.1. AF281255 mRNA. Translation: AAG59794.1. AY040274 mRNA. Translation: AAK72109.1. AK292995 mRNA. Translation: BAF85684.1. CH471094 Genomic DNA. Translation: EAW96247.1. BC025778 mRNA. Translation: AAH25778.1.
IPI	IPI00006162. IPI00032174. IPI00045049.
RefSeq	NP_110393.1. NM_030766.1. NP_620048.1. NM_138722.1. NP_620049.1. NM_138723.1.
UniGene	Hs.210343.
3D structure databases
ProteinModelPortal	Q9BZR8.
SMR	Q9BZR8. Positions 205-321.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BZR8. 1 interaction.
STRING	Q9BZR8.
PTM databases
PhosphoSite	Q9BZR8.
Polymorphism databases
DMDM	56748611.
Proteomic databases
PRIDE	Q9BZR8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000308721; ENSP00000309132; ENSG00000121380. ENST00000396367; ENSP00000379653; ENSG00000121380.
GeneID	79370.
KEGG	hsa:79370.
UCSC	uc001rac.1. human. uc001rae.1. human. uc001raf.1. human.
Organism-specific databases
CTD	79370.
GeneCards	GC12P012115.
HGNC	HGNC:16657. BCL2L14.
HPA	HPA040665.
MIM	606126. gene.
neXtProt	NX_Q9BZR8.
PharmGKB	PA134870762.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00390000007293.
HOGENOM	HBG126648.
HOVERGEN	HBG050649.
InParanoid	Q9BZR8.
OMA	KKDKALM.
PhylomeDB	Q9BZR8.
Gene expression databases
ArrayExpress	Q9BZR8.
Bgee	Q9BZR8.
CleanEx	HS_BCL2L14.
Genevestigator	Q9BZR8.
GermOnline	ENSG00000121380. Homo sapiens.
Family and domain databases
InterPro	IPR002475. Bcl2-like_apoptosis. IPR000712. Bcl2_BH. [Graphical view]
SMART	SM00337. BCL. 1 hit. [Graphical view]
PROSITE	PS50062. BCL2_FAMILY. 1 hit. PS01258. BH2. False negative. PS01259. BH3. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	68351.
SOURCE	Search...

Entry information

Entry name

B2L14_HUMAN

Accession

Primary (citable) accession number: Q9BZR8
Secondary accession number(s): A8KAD0, Q96QR5, Q9BZR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.