Q9BZR6 (RTN4R_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Reticulon-4 receptor

Alternative name(s):
Nogo receptor
Short name=NgR
Nogo-66 receptor

Gene names

Name:	RTN4R
Synonyms:	NOGOR
ORF Names:	UNQ330/PRO526

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for RTN4, OMG and MAG. Mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system. Acts in conjunction with RTN4 and LIGO1 in regulating neuronal precursor cell motility during cortical development By similarity. Ref.9 Ref.12
Subunit structure	Homomultimer. Interacts with LINGO1. Interacts with KIAA0319L. Ref.10 Ref.11 Ref.12 Ref.15
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Tissue specificity	Widespread in the brain but highest levels in the gray matter. Low levels in heart and kidney not expressed in oligodendrocytes (white matter).
Sequence similarities	Belongs to the Nogo receptor family. Contains 8 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Leucine-rich repeat Repeat Signal
Molecular function	Receptor
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	axonogenesis Non-traceable author statement Ref.1. Source: UniProtKB negative regulation of axonogenesis Traceable author statement. Source: Reactome nerve growth factor receptor signaling pathway Traceable author statement. Source: Reactome
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW cell surface Inferred from direct assay PubMed 20463223. Source: DFLAT endoplasmic reticulum Inferred from direct assay. Source: LIFEdb plasma membrane Traceable author statement. Source: Reactome
Molecular_function	receptor activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
KIAA0319L	Q8IZA0	4	EBI-5240240,EBI-5240269

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

26

Ref.8

Chain

27 – 447

421

Reticulon-4 receptor

PRO_0000022253

Propeptide

448 – 473

26

Removed in mature form Potential

PRO_0000022254

Regions

Domain

27 – 57

31

LRRNT

Repeat

58 – 79

22

LRR 1

Repeat

82 – 103

22

LRR 2

Repeat

106 – 128

23

LRR 3

Repeat

131 – 152

22

LRR 4

Repeat

155 – 176

22

LRR 5

Repeat

179 – 200

22

LRR 6

Repeat

203 – 224

22

LRR 7

Repeat

227 – 248

22

LRR 8

Domain

260 – 311

52

LRRCT

Compositional bias

435 – 442

8

Poly-Gly

Amino acid modifications

Lipidation

447

1

GPI-anchor amidated serine Potential

Glycosylation

82

1

N-linked (GlcNAc...) Ref.17

Glycosylation

179

1

N-linked (GlcNAc...) Ref.17

Disulfide bond

27 ↔ 33

Ref.16 Ref.17

Disulfide bond

31 ↔ 43

Ref.16 Ref.17

Disulfide bond

264 ↔ 287

Ref.16 Ref.17

Disulfide bond

266 ↔ 309

Ref.16 Ref.17

Secondary structure

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473

Helix Strand Turn

Details...

Beta strand

32 – 34

3

Beta strand

36 – 38

3

Beta strand

40 – 42

3

Beta strand

60 – 63

4

Turn

74 – 79

6

Beta strand

85 – 87

3

Turn

98 – 103

6

Beta strand

109 – 111

3

Turn

123 – 128

6

Beta strand

134 – 136

3

Turn

147 – 152

6

Beta strand

158 – 160

3

Turn

171 – 176

6

Beta strand

182 – 184

3

Turn

195 – 200

6

Beta strand

206 – 208

3

Turn

219 – 224

6

Beta strand

230 – 232

3

Helix

243 – 246

4

Beta strand

254 – 256

3

Helix

266 – 268

3

Helix

269 – 277

9

Beta strand

280 – 282

3

Beta strand

286 – 290

5

Helix

291 – 293

3

Helix

298 – 300

3

Helix

303 – 305

3

Sequences

	Sequence		Length	Mass (Da)
	Q9BZR6 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: CA5624B24C584702	FASTA	473	50,708
	10 20 30 40 50 60 MKRASAGGSR LLAWVLWLQA WQVAAPCPGA CVCYNEPKVT TSCPQQGLQA VPVGIPAASQ 70 80 90 100 110 120 RIFLHGNRIS HVPAASFRAC RNLTILWLHS NVLARIDAAA FTGLALLEQL DLSDNAQLRS 130 140 150 160 170 180 VDPATFHGLG RLHTLHLDRC GLQELGPGLF RGLAALQYLY LQDNALQALP DDTFRDLGNL 190 200 210 220 230 240 THLFLHGNRI SSVPERAFRG LHSLDRLLLH QNRVAHVHPH AFRDLGRLMT LYLFANNLSA 250 260 270 280 290 300 LPTEALAPLR ALQYLRLNDN PWVCDCRARP LWAWLQKFRG SSSEVPCSLP QRLAGRDLKR 310 320 330 340 350 360 LAANDLQGCA VATGPYHPIW TGRATDEEPL GLPKCCQPDA ADKASVLEPG RPASAGNALK 370 380 390 400 410 420 GRVPPGDSPP GNGSGPRHIN DSPFGTLPGS AEPPLTAVRP EGSEPPGFPT SGPRRRPGCS 430 440 450 460 470 RKNRTRSHCR LGQAGSGGGG TGDSEGSGAL PSLTCSLTPL GLALVLWTVL GPC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a receptor mediating Nogo-66 inhibition of axonal regeneration." Fournier A.E., GrandPre T., Strittmatter S.M. Nature 409:341-346(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence of human chromosome 22." Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M. , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H. Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[8]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-41.
[9]	"Nogo-66 receptor antagonist peptide promotes axonal regeneration." GrandPre T., Li S., Strittmatter S.M. Nature 417:547-551(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Oligodendrocyte-myelin glycoprotein is a Nogo receptor ligand that inhibits neurite outgrowth." Wang K.C., Koprivica V., Kim J.A., Sivasankaran R., Guo Y., Neve R.L., He Z. Nature 417:941-944(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH OMG.
[11]	"Myelin-associated glycoprotein as a functional ligand for the Nogo-66 receptor." Liu B.P., Fournier A., GrandPre T., Strittmatter S.M. Science 297:1190-1193(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MAG.
[12]	"LINGO-1 is a component of the Nogo-66 receptor/p75 signaling complex." Mi S., Lee X., Shao Z., Thill G., Ji B., Relton J., Levesque M., Allaire N., Perrin S., Sands B., Crowell T., Cate R.L., McCoy J.M., Pepinsky R.B. Nat. Neurosci. 7:221-228(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH LINGO1.
[13]	"It takes more than two to Nogo." Woolf C.J., Bloechlinger S. Science 297:1132-1134(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[14]	"Nogos and the Nogo-66 receptor: factors inhibiting CNS neuron regeneration." Ng C.E.L., Tang B.L. J. Neurosci. Res. 67:559-565(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[15]	"Dyslexia-associated kiaa0319-like protein interacts with axon guidance receptor nogo receptor 1." Poon M.W., Tsang W.H., Chan S.O., Li H.M., Ng H.K., Waye M.M. Cell. Mol. Neurobiol. 31:27-35(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH KIAA0319L.
[16]	"Structure and axon outgrowth inhibitor binding of the Nogo-66 receptor and related proteins." Barton W.A., Liu B.P., Tzvetkova D., Jeffrey P.D., Fournier A.E., Sah D., Cate R., Strittmatter S.M., Nikolov D.B. EMBO J. 22:3291-3302(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 27-311, DISULFIDE BONDS.
[17]	"Structure of the Nogo receptor ectodomain: a recognition module implicated in myelin inhibition." He X.L., Bazan J.F., McDermott G., Park J.B., Wang K., Tessier-Lavigne M., He Z., Garcia K.C. Neuron 38:177-185(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.52 ANGSTROMS) OF 26-310, DISULFIDE BONDS, GLYCOSYLATION AT ASN-82 AND ASN-179.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Nerve regrowth: nipped by a no-go - Issue 69 of April 2006

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF283463 mRNA. Translation: AAG53612.1.
AL834449 mRNA. Translation: CAD39109.1.
AY358297 mRNA. Translation: AAQ88664.1.
CR456360 mRNA. Translation: CAG30246.1.
AC058790 Genomic DNA. No translation available.
AC007663 Genomic DNA. No translation available.
CH471176 Genomic DNA. Translation: EAX02975.1.
CH471176 Genomic DNA. Translation: EAX02976.1.
BC011787 mRNA. Translation: AAH11787.1.

IPI

IPI00289204.

RefSeq

NP_075380.1. NM_023004.5.

UniGene

Hs.30868.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OZN	X-ray	1.52	A	26-310	[»]
1P8T	X-ray	3.20	A	27-311	[»]

ProteinModelPortal

Q9BZR6.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9BZR6. 1 interaction.

STRING

9606.ENSP00000043402.

Polymorphism databases

DMDM

25453267.

Proteomic databases

PaxDb

Q9BZR6.

PRIDE

Q9BZR6.

Protocols and materials databases

DNASU

65078.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000043402; ENSP00000043402; ENSG00000040608.

GeneID

65078.

KEGG

hsa:65078.

UCSC

uc002zru.3. human.

Organism-specific databases

CTD

65078.

GeneCards

GC22M020234.

HGNC

HGNC:18601. RTN4R.

HPA

CAB012443.

MIM

605566. gene.

neXtProt

NX_Q9BZR6.

PharmGKB

PA38600.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG4886.

HOGENOM

HOG000116109.

HOVERGEN

HBG063707.

InParanoid

Q9BZR6.

KO

K16659.

PhylomeDB

Q9BZR6.

Enzyme and pathway databases

Pathway_Interaction_DB

p75ntrpathway. p75(NTR)-mediated signaling.

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

Bgee

Q9BZR6.

CleanEx

HS_RTN4R.

Genevestigator

Q9BZR6.

GermOnline

ENSG00000040608. Homo sapiens.

Family and domain databases

InterPro

IPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRR-contain_N.
[Graphical view]

SMART

SM00369. LRR_TYP. 2 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]

PROSITE

PS51450. LRR. 6 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9BZR6.

GenomeRNAi

65078.

NextBio

67268.

SOURCE

Search...

Entry information

Entry name

RTN4R_HUMAN

Accession

Primary (citable) accession number: Q9BZR6
Secondary accession number(s): D3DX28

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.