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Q9BZQ6 (EDEM3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ER degradation-enhancing alpha-mannosidase-like protein 3

EC=3.2.1.113
Alternative name(s):
Alpha-1,2-mannosidase EDEM3
Gene names
Name:EDEM3
Synonyms:C1orf22
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length932 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in endoplasmic reticulum-associated degradation (ERAD). Accelerates the glycoprotein ERAD by proteasomes. This process depends on mannose-trimming from the N-glycans. Seems to have alpha 1,2-mannosidase activity By similarity.

Catalytic activity

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Subcellular location

Endoplasmic reticulum lumen By similarity.

Domain

Contains a protease-associated domain (PA) of unknown function.

Sequence similarities

Belongs to the glycosyl hydrolase 47 family.

Contains 1 PA (protease associated) domain.

Sequence caution

The sequence AAG60613.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAG37573.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4141 Potential
Chain42 – 932891ER degradation-enhancing alpha-mannosidase-like protein 3
PRO_0000210323

Regions

Domain674 – 779106PA
Motif929 – 9324Prevents secretion from ER Potential

Sites

Active site1461Proton donor By similarity
Active site2931 By similarity
Active site4051 By similarity

Amino acid modifications

Glycosylation1181N-linked (GlcNAc...) Potential
Glycosylation1951N-linked (GlcNAc...) Ref.6
Glycosylation5041N-linked (GlcNAc...) Potential
Glycosylation5111N-linked (GlcNAc...) Potential
Glycosylation8101N-linked (GlcNAc...) Potential
Glycosylation8141N-linked (GlcNAc...) Potential
Glycosylation9001N-linked (GlcNAc...) Potential

Natural variations

Natural variant8201I → S. Ref.5
Corresponds to variant rs9425343 [ dbSNP | Ensembl ].
VAR_059306

Experimental info

Sequence conflict2761I → T in BAG37573. Ref.1
Sequence conflict318 – 3192HY → VS in AAI05587. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9BZQ6 [UniParc].

Last modified February 5, 2008. Version 2.
Checksum: 3C040FDF48284D36

FASTA932104,664
        10         20         30         40         50         60 
MSEAGGRGCG SPVPQRARWR LVAATAAFCL VSATSVWTAG AEPMSREEKQ KLGNQVLEMF 

        70         80         90        100        110        120 
DHAYGNYMEH AYPADELMPL TCRGRVRGQE PSRGDVDDAL GKFSLTLIDS LDTLVVLNKT 

       130        140        150        160        170        180 
KEFEDAVRKV LRDVNLDNDV VVSVFETNIR VLGGLLGGHS LAIMLKEKGE YMQWYNDELL 

       190        200        210        220        230        240 
QMAKQLGYKL LPAFNTTSGL PYPRINLKFG IRKPEARTGT ETDTCTACAG TLILEFAALS 

       250        260        270        280        290        300 
RFTGATIFEE YARKALDFLW EKRQRSSNLV GVTINIHTGD WVRKDSGVGA GIDSYYEYLL 

       310        320        330        340        350        360 
KAYVLLGDDS FLERFNTHYD AIMRYISQPP LLLDVHIHKP MLNARTWMDA LLAFFPGLQV 

       370        380        390        400        410        420 
LKGDIRPAIE THEMLYQVIK KHNFLPEAFT TDFRVHWAQH PLRPEFAEST YFLYKATGDP 

       430        440        450        460        470        480 
YYLEVGKTLI ENLNKYARVP CGFAAMKDVR TGSHEDRMDS FFLAEMFKYL YLLFADKEDI 

       490        500        510        520        530        540 
IFDIEDYIFT TEAHLLPLWL STTNQSISKK NTTSEYTELD DSNFDWTCPN TQILFPNDPL 

       550        560        570        580        590        600 
YAQSIREPLK NVVDKSCPRG IIRVEESFRS GAKPPLRARD FMATNPEHLE ILKKMGVSLI 

       610        620        630        640        650        660 
HLKDGRVQLV QHAIQAASSI DAEDGLRFMQ EMIELSSQQQ KEQQLPPRAV QIVSHPFFGR 

       670        680        690        700        710        720 
VVLTAGPAQF GLDLSKHKET RGFVASSKPS NGCSELTNPE AVMGKIALIQ RGQCMFAEKA 

       730        740        750        760        770        780 
RNIQNAGAIG GIVIDDNEGS SSDTAPLFQM AGDGKDTDDI KIPMLFLFSK EGSIILDAIR 

       790        800        810        820        830        840 
EYEEVEVLLS DKAKDRDPEM ENEEQPSSEN DSQNQSGEQI SSSSQEVDLV DQESSEENSL 

       850        860        870        880        890        900 
NSHPESLSLA DMDNAASISP SEQTSNPTEN HETTNLNGEC TDLDNQLQEQ SETEEDSNPN 

       910        920        930 
VSWGKKVQPI DSILADWNED IEAFEMMEKD EL 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[2]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Cloning and characterization of 13 novel transcripts and the human RGS8 gene from the 1q25 region encompassing the hereditary prostate cancer (HPC1) locus."
Sood R., Bonner T.I., Malakowska I., Stephan D.A., Robbins C.M., Connors T.D., Morgenbesser S.D., Su K., Faruque M.U., Pinkett H., Graham C., Baxevanis A.D., Klinger K.W., Landes G.M., Trent J.M., Carpten J.D.
Genomics 73:211-222(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 27-932.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 78-319.
Tissue: Lung.
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 553-932, VARIANT SER-820.
Tissue: Testis.
[6]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-195.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK315118 mRNA. Translation: BAG37573.1. Different initiation.
AL096819 Genomic DNA. Translation: CAI21741.1.
AF288393 mRNA. Translation: AAG60613.1. Different initiation.
BC105586 mRNA. Translation: AAI05587.1.
AL117441 mRNA. Translation: CAB55926.1.
PIRT17236.
RefSeqNP_079467.3. NM_025191.3.
UniGeneHs.523811.

3D structure databases

ProteinModelPortalQ9BZQ6.
SMRQ9BZQ6. Positions 56-499, 674-736.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123207. 4 interactions.
IntActQ9BZQ6. 2 interactions.
STRING9606.ENSP00000318147.

Protein family/group databases

CAZyGH47. Glycoside Hydrolase Family 47.

PTM databases

PhosphoSiteQ9BZQ6.

Polymorphism databases

DMDM166897965.

Proteomic databases

PaxDbQ9BZQ6.
PRIDEQ9BZQ6.

Protocols and materials databases

DNASU80267.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000318130; ENSP00000318147; ENSG00000116406.
GeneID80267.
KEGGhsa:80267.
UCSCuc010pok.2. human.

Organism-specific databases

CTD80267.
GeneCardsGC01M184659.
H-InvDBHIX0001417.
HGNCHGNC:16787. EDEM3.
HPAHPA025754.
HPA025755.
MIM610214. gene.
neXtProtNX_Q9BZQ6.
PharmGKBPA38186.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG307610.
HOGENOMHOG000007735.
HOVERGENHBG107835.
InParanoidQ9BZQ6.
KOK10086.
OMADRDPEME.
OrthoDBEOG79W94G.
PhylomeDBQ9BZQ6.
TreeFamTF300807.

Enzyme and pathway databases

ReactomeREACT_17015. Metabolism of proteins.

Gene expression databases

ArrayExpressQ9BZQ6.
BgeeQ9BZQ6.
CleanExHS_EDEM3.
GenevestigatorQ9BZQ6.

Family and domain databases

Gene3D1.50.10.50. 1 hit.
InterProIPR001382. Glyco_hydro_47.
IPR003137. Protease-assoc_domain.
[Graphical view]
PANTHERPTHR11742. PTHR11742. 1 hit.
PfamPF01532. Glyco_hydro_47. 1 hit.
PF02225. PA. 1 hit.
[Graphical view]
PRINTSPR00747. GLYHDRLASE47.
SUPFAMSSF48225. SSF48225. 1 hit.
PROSITEPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSEDEM3. human.
GeneWikiEDEM3.
GenomeRNAi80267.
NextBio70729.
PROQ9BZQ6.
SOURCESearch...

Entry information

Entry nameEDEM3_HUMAN
AccessionPrimary (citable) accession number: Q9BZQ6
Secondary accession number(s): B2RCH6 expand/collapse secondary AC list , Q0VGM5, Q5TEZ0, Q9HCW1, Q9UFV7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: February 5, 2008
Last modified: April 16, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries