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Protein

Acidic mammalian chitinase

Gene

CHIA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding.4 Publications

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei140Proton donorCurated1
Binding sitei141ChitooligosaccharideBy similarity1
Binding sitei360ChitooligosaccharideBy similarity1

GO - Molecular functioni

  • carbohydrate binding Source: UniProtKB
  • chitinase activity Source: UniProtKB
  • chitin binding Source: UniProtKB
  • kinase binding Source: UniProtKB
  • lysozyme activity Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cell wall chitin metabolic process Source: UniProtKB
  • chitin catabolic process Source: UniProtKB
  • chitin metabolic process Source: UniProtKB
  • digestion Source: UniProtKB
  • immune response Source: UniProtKB
  • polysaccharide catabolic process Source: UniProtKB-KW
  • polysaccharide digestion Source: Reactome
  • positive regulation of chemokine secretion Source: UniProtKB
  • production of molecular mediator involved in inflammatory response Source: UniProtKB
  • response to fungus Source: UniProtKB

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processApoptosis, Carbohydrate metabolism, Chitin degradation, Immunity, Inflammatory response, Polysaccharide degradation
LigandChitin-binding

Enzyme and pathway databases

BRENDAi3.2.1.14 2681
ReactomeiR-HSA-189085 Digestion of dietary carbohydrate

Protein family/group databases

CAZyiCBM14 Carbohydrate-Binding Module Family 14
GH18 Glycoside Hydrolase Family 18

Names & Taxonomyi

Protein namesi
Recommended name:
Acidic mammalian chitinase (EC:3.2.1.14)
Short name:
AMCase
Alternative name(s):
Lung-specific protein TSA1902
Gene namesi
Name:CHIA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000134216.18
HGNCiHGNC:17432 CHIA
MIMi606080 gene
neXtProtiNX_Q9BZP6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi138D → A: Loss of chitinase activity. No effect on protection against apoptosis or on AKT1 activation. 1 Publication1

Organism-specific databases

DisGeNETi27159
OpenTargetsiENSG00000134216
PharmGKBiPA142672117

Chemistry databases

ChEMBLiCHEMBL1293197
GuidetoPHARMACOLOGYi2982

Polymorphism and mutation databases

BioMutaiCHIA
DMDMi37999771

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21By similarityAdd BLAST21
ChainiPRO_000001194422 – 476Acidic mammalian chitinaseAdd BLAST455

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 51PROSITE-ProRule annotation1 Publication
Disulfide bondi49 ↔ 394PROSITE-ProRule annotation1 Publication
Disulfide bondi307 ↔ 372PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9BZP6
PeptideAtlasiQ9BZP6
PRIDEiQ9BZP6

PTM databases

iPTMnetiQ9BZP6
PhosphoSitePlusiQ9BZP6

Expressioni

Tissue specificityi

Detected in lung epithelial cells from asthma patients (at protein level). Highly expressed in stomach. Detected at lower levels in lung.2 Publications

Inductioni

Up-regulated in lung epithelial cells from asthma patients.1 Publication

Gene expression databases

BgeeiENSG00000134216
ExpressionAtlasiQ9BZP6 baseline and differential
GenevisibleiQ9BZP6 HS

Organism-specific databases

HPAiHPA059193

Interactioni

Subunit structurei

Interacts with EGFR.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TBC1D5Q926094EBI-14357960,EBI-742381

GO - Molecular functioni

  • kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi11803918 interactors.
IntActiQ9BZP6 2 interactors.
STRINGi9606.ENSP00000341828

Chemistry databases

BindingDBiQ9BZP6

Structurei

Secondary structure

1476
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 29Combined sources7
Helixi32 – 34Combined sources3
Helixi37 – 39Combined sources3
Helixi43 – 45Combined sources3
Turni48 – 50Combined sources3
Beta strandi52 – 62Combined sources11
Beta strandi65 – 67Combined sources3
Helixi73 – 82Combined sources10
Helixi83 – 85Combined sources3
Beta strandi91 – 97Combined sources7
Helixi99 – 101Combined sources3
Helixi104 – 110Combined sources7
Helixi113 – 130Combined sources18
Beta strandi133 – 138Combined sources6
Helixi151 – 173Combined sources23
Beta strandi179 – 184Combined sources6
Helixi188 – 194Combined sources7
Helixi197 – 203Combined sources7
Beta strandi205 – 209Combined sources5
Helixi217 – 219Combined sources3
Helixi236 – 240Combined sources5
Helixi243 – 252Combined sources10
Helixi257 – 259Combined sources3
Beta strandi260 – 275Combined sources16
Beta strandi284 – 288Combined sources5
Turni293 – 295Combined sources3
Beta strandi300 – 302Combined sources3
Helixi303 – 311Combined sources9
Beta strandi315 – 319Combined sources5
Turni320 – 323Combined sources4
Beta strandi324 – 329Combined sources6
Beta strandi332 – 335Combined sources4
Helixi339 – 351Combined sources13
Beta strandi355 – 360Combined sources6
Helixi362 – 364Combined sources3
Beta strandi367 – 369Combined sources3
Turni370 – 372Combined sources3
Helixi378 – 386Combined sources9
Helixi392 – 394Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YBTX-ray2.22A/B/C/D/E/F21-398[»]
2YBUX-ray2.25A/B/C/D/E/F21-398[»]
3FXYX-ray2.00A/B/C/D22-408[»]
3FY1X-ray1.70A/B22-408[»]
3RM4X-ray1.90A/B22-408[»]
3RM8X-ray1.80A/B22-408[»]
3RM9X-ray2.10A/B22-408[»]
3RMEX-ray1.80A/B22-408[»]
ProteinModelPortaliQ9BZP6
SMRiQ9BZP6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BZP6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini427 – 476Chitin-binding type-2PROSITE-ProRule annotationAdd BLAST50

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 71Chitooligosaccharide bindingCurated2
Regioni97 – 100Chitooligosaccharide bindingCurated4
Regioni210 – 213Chitooligosaccharide bindingCurated4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi415 – 420Poly-Ser6

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2806 Eukaryota
COG3325 LUCA
GeneTreeiENSGT00550000074323
HOVERGENiHBG011684
InParanoidiQ9BZP6
KOiK01183
OMAiTYSPNFA
OrthoDBiEOG091G014W
PhylomeDBiQ9BZP6
TreeFamiTF315610

Family and domain databases

Gene3Di3.10.50.101 hit
InterProiView protein in InterPro
IPR002557 Chitin-bd_dom
IPR036508 Chitin-bd_dom_sf
IPR011583 Chitinase_II
IPR029070 Chitinase_insertion_sf
IPR001223 Glyco_hydro18_cat
IPR001579 Glyco_hydro_18_chit_AS
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF01607 CBM_14, 1 hit
PF00704 Glyco_hydro_18, 1 hit
SMARTiView protein in SMART
SM00494 ChtBD2, 1 hit
SM00636 Glyco_18, 1 hit
SUPFAMiSSF51445 SSF51445, 2 hits
SSF54556 SSF54556, 1 hit
SSF57625 SSF57625, 1 hit
PROSITEiView protein in PROSITE
PS50940 CHIT_BIND_II, 1 hit
PS01095 CHITINASE_18, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BZP6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTKLILLTGL VLILNLQLGS AYQLTCYFTN WAQYRPGLGR FMPDNIDPCL
60 70 80 90 100
CTHLIYAFAG RQNNEITTIE WNDVTLYQAF NGLKNKNSQL KTLLAIGGWN
110 120 130 140 150
FGTAPFTAMV STPENRQTFI TSVIKFLRQY EFDGLDFDWE YPGSRGSPPQ
160 170 180 190 200
DKHLFTVLVQ EMREAFEQEA KQINKPRLMV TAAVAAGISN IQSGYEIPQL
210 220 230 240 250
SQYLDYIHVM TYDLHGSWEG YTGENSPLYK YPTDTGSNAY LNVDYVMNYW
260 270 280 290 300
KDNGAPAEKL IVGFPTYGHN FILSNPSNTG IGAPTSGAGP AGPYAKESGI
310 320 330 340 350
WAYYEICTFL KNGATQGWDA PQEVPYAYQG NVWVGYDNIK SFDIKAQWLK
360 370 380 390 400
HNKFGGAMVW AIDLDDFTGT FCNQGKFPLI STLKKALGLQ SASCTAPAQP
410 420 430 440 450
IEPITAAPSG SGNGSGSSSS GGSSGGSGFC AVRANGLYPV ANNRNAFWHC
460 470
VNGVTYQQNC QAGLVFDTSC DCCNWA
Length:476
Mass (Da):52,271
Last modified:June 1, 2001 - v1
Checksum:i92B27BAD2F7EB4CC
GO
Isoform 2 (identifier: Q9BZP6-2) [UniParc]FASTAAdd to basket
Also known as: TSA1902-L

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.

Show »
Length:368
Mass (Da):40,139
Checksum:iFC2558C4CEA48609
GO
Isoform 3 (identifier: Q9BZP6-3) [UniParc]FASTAAdd to basket
Also known as: TSA1902-S

The sequence of this isoform differs from the canonical sequence as follows:
     1-161: Missing.

Show »
Length:315
Mass (Da):33,906
Checksum:i61D614709B577CAD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203Y → C in AAX81431 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06303045N → D Increased chitinase activity; when associated with N-47 and M-61. 1 PublicationCorresponds to variant dbSNP:rs41282492Ensembl.1
Natural variantiVAR_06303147D → N Increased chitinase activity; when associated with D-45 and M-61. 1 PublicationCorresponds to variant dbSNP:rs41282494Ensembl.1
Natural variantiVAR_06303261R → M Increased chitinase activity; when associated with D-45 and N-47. 1 PublicationCorresponds to variant dbSNP:rs41282496Ensembl.1
Natural variantiVAR_049192102G → R1 PublicationCorresponds to variant dbSNP:rs3818822Ensembl.1
Natural variantiVAR_063033125K → R1 PublicationCorresponds to variant dbSNP:rs61756687Ensembl.1
Natural variantiVAR_033730324V → G. Corresponds to variant dbSNP:rs2256721Ensembl.1
Natural variantiVAR_049193339I → V2 PublicationsCorresponds to variant dbSNP:rs2275253Ensembl.1
Natural variantiVAR_049194354F → S1 PublicationCorresponds to variant dbSNP:rs2275254Ensembl.1
Natural variantiVAR_049195377F → L. Corresponds to variant dbSNP:rs36011905Ensembl.1
Natural variantiVAR_049196432V → G2 PublicationsCorresponds to variant dbSNP:rs2256721Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0086341 – 161Missing in isoform 3. 2 PublicationsAdd BLAST161
Alternative sequenceiVSP_0086351 – 108Missing in isoform 2. 3 PublicationsAdd BLAST108

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025008 mRNA Translation: BAA86980.1
AB025009 mRNA Translation: BAA86981.1
AF290004 mRNA Translation: AAG60019.1
AY789444 mRNA Translation: AAX81431.1
AY789445 mRNA Translation: AAX81432.1
AL513202 Genomic DNA No translation available.
AL356387 Genomic DNA No translation available.
BC047336 mRNA Translation: AAH47336.2
BC036339 mRNA Translation: AAH36339.2
BC106910 mRNA Translation: AAI06911.1
CCDSiCCDS41368.1 [Q9BZP6-1]
CCDS58017.1 [Q9BZP6-3]
CCDS832.1 [Q9BZP6-2]
RefSeqiNP_001035713.1, NM_001040623.2 [Q9BZP6-3]
NP_001244930.1, NM_001258001.1 [Q9BZP6-2]
NP_001244931.1, NM_001258002.1 [Q9BZP6-3]
NP_001244932.1, NM_001258003.1 [Q9BZP6-2]
NP_001244933.1, NM_001258004.1 [Q9BZP6-3]
NP_001244934.1, NM_001258005.1 [Q9BZP6-3]
NP_068569.2, NM_021797.3 [Q9BZP6-2]
NP_970615.2, NM_201653.3 [Q9BZP6-1]
XP_006710640.1, XM_006710577.3 [Q9BZP6-3]
XP_016856536.1, XM_017001047.1 [Q9BZP6-3]
XP_016856537.1, XM_017001048.1 [Q9BZP6-2]
UniGeneiHs.128814

Genome annotation databases

EnsembliENST00000343320; ENSP00000341828; ENSG00000134216 [Q9BZP6-1]
ENST00000353665; ENSP00000338970; ENSG00000134216 [Q9BZP6-3]
ENST00000369740; ENSP00000358755; ENSG00000134216 [Q9BZP6-1]
ENST00000430615; ENSP00000391132; ENSG00000134216 [Q9BZP6-2]
ENST00000451398; ENSP00000390476; ENSG00000134216 [Q9BZP6-3]
ENST00000483391; ENSP00000436946; ENSG00000134216 [Q9BZP6-3]
GeneIDi27159
KEGGihsa:27159
UCSCiuc001eas.5 human [Q9BZP6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCHIA_HUMAN
AccessioniPrimary (citable) accession number: Q9BZP6
Secondary accession number(s): Q32W79
, Q32W80, Q3B866, Q5U5Z5, Q5VUV4, Q86UD8, Q9ULY3, Q9ULY4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome