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Q9BZM4

- N2DL3_HUMAN

UniProt

Q9BZM4 - N2DL3_HUMAN

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Protein

NKG2D ligand 3

Gene
ULBP3, N2DL3, RAET1N
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Ligand for the KLRK1/NKG2D receptor, together with at least ULBP1 and ULBP2. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to KLRK1/NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. Has lower affinity for KLRK1/NKG2D compared to ULBP1 and ULBP2 and induces weaker signaling responses than does ULBP2 or ULBP1.1 Publication

GO - Molecular functioni

  1. antigen binding Source: RefGenome
  2. natural killer cell lectin-like receptor binding Source: UniProtKB

GO - Biological processi

  1. antigen processing and presentation Source: RefGenome
  2. natural killer cell activation Source: UniProtKB
  3. natural killer cell mediated cytotoxicity Source: UniProtKB
  4. regulation of immune response Source: Reactome
  5. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Enzyme and pathway databases

ReactomeiREACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Names & Taxonomyi

Protein namesi
Recommended name:
NKG2D ligand 3
Short name:
N2DL-3
Short name:
NKG2DL3
Alternative name(s):
ALCAN-gamma
Retinoic acid early transcript 1N
Gene namesi
Name:ULBP3
Synonyms:N2DL3, RAET1N
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 6

Organism-specific databases

HGNCiHGNC:14895. ULBP3.

Subcellular locationi

Cell membrane; Lipid-anchorGPI-anchor 1 Publication

GO - Cellular componenti

  1. anchored component of plasma membrane Source: UniProtKB
  2. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37917.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Reviewed predictionAdd
BLAST
Chaini30 – 217188NKG2D ligand 3PRO_0000019019Add
BLAST
Propeptidei218 – 24427Removed in mature form Reviewed predictionPRO_0000019020Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi37 – 371N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi51 ↔ 67
Disulfide bondi128 ↔ 191
Lipidationi217 – 2171GPI-anchor amidated glycine Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ9BZM4.
PaxDbiQ9BZM4.
PRIDEiQ9BZM4.

Expressioni

Gene expression databases

BgeeiQ9BZM4.
CleanExiHS_ULBP3.
GenevestigatoriQ9BZM4.

Organism-specific databases

HPAiHPA046558.
HPA063007.

Interactioni

Subunit structurei

Does not bind to beta2-microglobulin. Interacts with human cytomegalovirus/HHV-5 protein UL142.2 Publications

Protein-protein interaction databases

IntActiQ9BZM4. 1 interaction.
STRINGi9606.ENSP00000253335.

Structurei

Secondary structure

1
244
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi32 – 4312
Beta strandi50 – 578
Beta strandi60 – 667
Turni67 – 704
Beta strandi83 – 853
Helixi86 – 10722
Beta strandi121 – 13010
Beta strandi137 – 15317
Turni154 – 1574
Beta strandi158 – 1636
Helixi164 – 1663
Helixi167 – 1759
Helixi177 – 18812
Helixi190 – 20617

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KCGX-ray2.60C30-207[»]
ProteinModelPortaliQ9BZM4.
SMRiQ9BZM4. Positions 30-207.

Miscellaneous databases

EvolutionaryTraceiQ9BZM4.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni30 – 11889MHC class I alpha-1 likeAdd
BLAST
Regioni119 – 20991MHC class I alpha-2 likeAdd
BLAST

Sequence similaritiesi

Belongs to the MHC class I family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG75885.
HOGENOMiHOG000070028.
HOVERGENiHBG052564.
InParanoidiQ9BZM4.
KOiK07986.
OMAiWGKQLEM.
OrthoDBiEOG7HB59Z.
PhylomeDBiQ9BZM4.
TreeFamiTF341724.

Family and domain databases

Gene3Di3.30.500.10. 1 hit.
InterProiIPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
[Graphical view]
PfamiPF00129. MHC_I. 1 hit.
[Graphical view]
SUPFAMiSSF54452. SSF54452. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BZM4-1 [UniParc]FASTAAdd to Basket

« Hide

MAAAASPAIL PRLAILPYLL FDWSGTGRAD AHSLWYNFTI IHLPRHGQQW    50
CEVQSQVDQK NFLSYDCGSD KVLSMGHLEE QLYATDAWGK QLEMLREVGQ 100
RLRLELADTE LEDFTPSGPL TLQVRMSCEC EADGYIRGSW QFSFDGRKFL 150
LFDSNNRKWT VVHAGARRMK EKWEKDSGLT TFFKMVSMRD CKSWLRDFLM 200
HRKKRLEPTA PPTMAPGLAQ PKAIATTLSP WSFLIILCFI LPGI 244
Length:244
Mass (Da):27,949
Last modified:June 1, 2001 - v1
Checksum:iBAF07895640B9901
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF304379 mRNA. Translation: AAK13083.1.
AB052908 mRNA. Translation: BAB61050.1.
AK315275 mRNA. Translation: BAG37687.1.
AL355497 Genomic DNA. Translation: CAH74128.1.
CH471051 Genomic DNA. Translation: EAW47772.1.
BC098127 mRNA. Translation: AAH98127.1.
AF346596 mRNA. Translation: AAN40839.1.
AY027538 mRNA. Translation: AAK13049.1.
CCDSiCCDS5225.1.
RefSeqiNP_078794.1. NM_024518.1.
XP_005267194.1. XM_005267137.2.
UniGeneiHs.326556.

Genome annotation databases

EnsembliENST00000367339; ENSP00000356308; ENSG00000131019.
ENST00000438272; ENSP00000403562; ENSG00000131019.
GeneIDi79465.
KEGGihsa:79465.
UCSCiuc003qns.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF304379 mRNA. Translation: AAK13083.1 .
AB052908 mRNA. Translation: BAB61050.1 .
AK315275 mRNA. Translation: BAG37687.1 .
AL355497 Genomic DNA. Translation: CAH74128.1 .
CH471051 Genomic DNA. Translation: EAW47772.1 .
BC098127 mRNA. Translation: AAH98127.1 .
AF346596 mRNA. Translation: AAN40839.1 .
AY027538 mRNA. Translation: AAK13049.1 .
CCDSi CCDS5225.1.
RefSeqi NP_078794.1. NM_024518.1.
XP_005267194.1. XM_005267137.2.
UniGenei Hs.326556.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KCG X-ray 2.60 C 30-207 [» ]
ProteinModelPortali Q9BZM4.
SMRi Q9BZM4. Positions 30-207.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9BZM4. 1 interaction.
STRINGi 9606.ENSP00000253335.

Proteomic databases

MaxQBi Q9BZM4.
PaxDbi Q9BZM4.
PRIDEi Q9BZM4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000367339 ; ENSP00000356308 ; ENSG00000131019 .
ENST00000438272 ; ENSP00000403562 ; ENSG00000131019 .
GeneIDi 79465.
KEGGi hsa:79465.
UCSCi uc003qns.3. human.

Organism-specific databases

CTDi 79465.
GeneCardsi GC06M150477.
HGNCi HGNC:14895. ULBP3.
HPAi HPA046558.
HPA063007.
MIMi 605699. gene.
neXtProti NX_Q9BZM4.
PharmGKBi PA37917.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG75885.
HOGENOMi HOG000070028.
HOVERGENi HBG052564.
InParanoidi Q9BZM4.
KOi K07986.
OMAi WGKQLEM.
OrthoDBi EOG7HB59Z.
PhylomeDBi Q9BZM4.
TreeFami TF341724.

Enzyme and pathway databases

Reactomei REACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Miscellaneous databases

EvolutionaryTracei Q9BZM4.
GeneWikii ULBP3.
GenomeRNAii 79465.
NextBioi 68421.
PROi Q9BZM4.
SOURCEi Search...

Gene expression databases

Bgeei Q9BZM4.
CleanExi HS_ULBP3.
Genevestigatori Q9BZM4.

Family and domain databases

Gene3Di 3.30.500.10. 1 hit.
InterProi IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
[Graphical view ]
Pfami PF00129. MHC_I. 1 hit.
[Graphical view ]
SUPFAMi SSF54452. SSF54452. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "ULBPs, novel MHC class I-related molecules, bind to CMV glycoprotein UL16 and stimulate NK cytotoxicity through the NKG2D receptor."
    Cosman D., Mullberg J., Sutherland C.L., Chin W., Armitage R., Fanslow W., Kubin M., Chalupny N.J.
    Immunity 14:123-133(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GPI-ANCHOR.
  2. Onda H., Shintani Y., Ohkubo S., Ogi K.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Interactions of human NKG2D with its ligands MICA, MICB, and homologs of the mouse RAE-1 protein family."
    Steinle A., Li P., Morris D.L., Groh V., Lanier L.L., Strong R.K., Spies T.
    Immunogenetics 53:279-287(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-244, INTERACTION WITH KLRK1.
  8. "A cluster of ten novel MHC class I related genes on human chromosome 6q24.2-q25.3."
    Radosavljevic M., Cuillerier B., Wilson M.J., Clement O., Wicker S., Gilfillan S., Beck S., Trowsdale J., Bahram S.
    Genomics 79:114-123(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-244.
  9. "UL16-binding proteins, novel MHC class I-related proteins, bind to NKG2D and activate multiple signaling pathways in primary NK cells."
    Sutherland C.L., Chalupny N.J., Schooley K., VandenBos T., Kubin M., Cosman D.
    J. Immunol. 168:671-679(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A LIGAND FOR KLRK1.
  10. "NKG2D ligands: unconventional MHC class I-like molecules exploited by viruses and cancer."
    Cerwenka A., Lanier L.L.
    Tissue Antigens 61:335-343(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  11. "Intracellular sequestration of the NKG2D ligand ULBP3 by human cytomegalovirus."
    Bennett N.J., Ashiru O., Morgan F.J., Pang Y., Okecha G., Eagle R.A., Trowsdale J., Sissons J.G., Wills M.R.
    J. Immunol. 185:1093-1102(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HHV-5 PROTEIN UL142.
  12. "Conformational plasticity revealed by the cocrystal structure of NKG2D and its class I MHC-like ligand ULBP3."
    Radaev S., Rostro B., Brooks A.G., Colonna M., Sun P.D.
    Immunity 15:1039-1049(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 30-207 IN COMPLEX WITH THE EXTRACELLULAR DOMAIN OF KLRK1.

Entry informationi

Entry nameiN2DL3_HUMAN
AccessioniPrimary (citable) accession number: Q9BZM4
Secondary accession number(s): Q5VY82, Q8IZX5, Q8TE75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: June 1, 2001
Last modified: September 3, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The ULBPs are unusual members of the extended MHC class I superfamily, because they do not contain the alpha 3 domain and they lack a transmembrane domain. They are unlikely to present peptides.
The region from chromosome 6q24.1-6q25.3 contains as many as 10 ULBP-related sequences, many of which are pseudogenes.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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