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Q9BZM4 (N2DL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NKG2D ligand 3

Short name=N2DL-3
Short name=NKG2DL3
Alternative name(s):
ALCAN-gamma
Retinoic acid early transcript 1N
Gene names
Name:ULBP3
Synonyms:N2DL3, RAET1N
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length244 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ligand for the KLRK1/NKG2D receptor, together with at least ULBP1 and ULBP2. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to KLRK1/NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. Has lower affinity for KLRK1/NKG2D compared to ULBP1 and ULBP2 and induces weaker signaling responses than does ULBP2 or ULBP1. Ref.9

Subunit structure

Does not bind to beta2-microglobulin. Interacts with human cytomegalovirus/HHV-5 protein UL142. Ref.7 Ref.11

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor Ref.1.

Miscellaneous

The ULBPs are unusual members of the extended MHC class I superfamily, because they do not contain the alpha 3 domain and they lack a transmembrane domain. They are unlikely to present peptides.

The region from chromosome 6q24.1-6q25.3 contains as many as 10 ULBP-related sequences, many of which are pseudogenes.

Sequence similarities

Belongs to the MHC class I family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 217188NKG2D ligand 3
PRO_0000019019
Propeptide218 – 24427Removed in mature form Potential
PRO_0000019020

Regions

Region30 – 11889MHC class I alpha-1 like
Region119 – 20991MHC class I alpha-2 like

Amino acid modifications

Lipidation2171GPI-anchor amidated glycine Potential
Glycosylation371N-linked (GlcNAc...) Potential
Disulfide bond51 ↔ 67
Disulfide bond128 ↔ 191

Secondary structure

....................... 244
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9BZM4 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: BAF07895640B9901

FASTA24427,949
        10         20         30         40         50         60 
MAAAASPAIL PRLAILPYLL FDWSGTGRAD AHSLWYNFTI IHLPRHGQQW CEVQSQVDQK 

        70         80         90        100        110        120 
NFLSYDCGSD KVLSMGHLEE QLYATDAWGK QLEMLREVGQ RLRLELADTE LEDFTPSGPL 

       130        140        150        160        170        180 
TLQVRMSCEC EADGYIRGSW QFSFDGRKFL LFDSNNRKWT VVHAGARRMK EKWEKDSGLT 

       190        200        210        220        230        240 
TFFKMVSMRD CKSWLRDFLM HRKKRLEPTA PPTMAPGLAQ PKAIATTLSP WSFLIILCFI 


LPGI 

« Hide

References

« Hide 'large scale' references
[1]"ULBPs, novel MHC class I-related molecules, bind to CMV glycoprotein UL16 and stimulate NK cytotoxicity through the NKG2D receptor."
Cosman D., Mullberg J., Sutherland C.L., Chin W., Armitage R., Fanslow W., Kubin M., Chalupny N.J.
Immunity 14:123-133(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GPI-ANCHOR.
[2]Onda H., Shintani Y., Ohkubo S., Ogi K.
Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[4]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"Interactions of human NKG2D with its ligands MICA, MICB, and homologs of the mouse RAE-1 protein family."
Steinle A., Li P., Morris D.L., Groh V., Lanier L.L., Strong R.K., Spies T.
Immunogenetics 53:279-287(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-244, INTERACTION WITH KLRK1.
[8]"A cluster of ten novel MHC class I related genes on human chromosome 6q24.2-q25.3."
Radosavljevic M., Cuillerier B., Wilson M.J., Clement O., Wicker S., Gilfillan S., Beck S., Trowsdale J., Bahram S.
Genomics 79:114-123(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-244.
[9]"UL16-binding proteins, novel MHC class I-related proteins, bind to NKG2D and activate multiple signaling pathways in primary NK cells."
Sutherland C.L., Chalupny N.J., Schooley K., VandenBos T., Kubin M., Cosman D.
J. Immunol. 168:671-679(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A LIGAND FOR KLRK1.
[10]"NKG2D ligands: unconventional MHC class I-like molecules exploited by viruses and cancer."
Cerwenka A., Lanier L.L.
Tissue Antigens 61:335-343(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[11]"Intracellular sequestration of the NKG2D ligand ULBP3 by human cytomegalovirus."
Bennett N.J., Ashiru O., Morgan F.J., Pang Y., Okecha G., Eagle R.A., Trowsdale J., Sissons J.G., Wills M.R.
J. Immunol. 185:1093-1102(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HHV-5 PROTEIN UL142.
[12]"Conformational plasticity revealed by the cocrystal structure of NKG2D and its class I MHC-like ligand ULBP3."
Radaev S., Rostro B., Brooks A.G., Colonna M., Sun P.D.
Immunity 15:1039-1049(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 30-207 IN COMPLEX WITH THE EXTRACELLULAR DOMAIN OF KLRK1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF304379 mRNA. Translation: AAK13083.1.
AB052908 mRNA. Translation: BAB61050.1.
AK315275 mRNA. Translation: BAG37687.1.
AL355497 Genomic DNA. Translation: CAH74128.1.
CH471051 Genomic DNA. Translation: EAW47772.1.
BC098127 mRNA. Translation: AAH98127.1.
AF346596 mRNA. Translation: AAN40839.1.
AY027538 mRNA. Translation: AAK13049.1.
CCDSCCDS5225.1.
RefSeqNP_078794.1. NM_024518.1.
XP_005267194.1. XM_005267137.2.
UniGeneHs.326556.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1KCGX-ray2.60C30-207[»]
ProteinModelPortalQ9BZM4.
SMRQ9BZM4. Positions 30-207.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9BZM4. 1 interaction.
STRING9606.ENSP00000253335.

Proteomic databases

MaxQBQ9BZM4.
PaxDbQ9BZM4.
PRIDEQ9BZM4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000367339; ENSP00000356308; ENSG00000131019.
ENST00000438272; ENSP00000403562; ENSG00000131019.
GeneID79465.
KEGGhsa:79465.
UCSCuc003qns.3. human.

Organism-specific databases

CTD79465.
GeneCardsGC06M150477.
HGNCHGNC:14895. ULBP3.
HPAHPA046558.
HPA063007.
MIM605699. gene.
neXtProtNX_Q9BZM4.
PharmGKBPA37917.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG75885.
HOGENOMHOG000070028.
HOVERGENHBG052564.
InParanoidQ9BZM4.
KOK07986.
OMAWGKQLEM.
OrthoDBEOG7HB59Z.
PhylomeDBQ9BZM4.
TreeFamTF341724.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

BgeeQ9BZM4.
CleanExHS_ULBP3.
GenevestigatorQ9BZM4.

Family and domain databases

Gene3D3.30.500.10. 1 hit.
InterProIPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
[Graphical view]
PfamPF00129. MHC_I. 1 hit.
[Graphical view]
SUPFAMSSF54452. SSF54452. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ9BZM4.
GeneWikiULBP3.
GenomeRNAi79465.
NextBio68421.
PROQ9BZM4.
SOURCESearch...

Entry information

Entry nameN2DL3_HUMAN
AccessionPrimary (citable) accession number: Q9BZM4
Secondary accession number(s): Q5VY82, Q8IZX5, Q8TE75
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM