Q9BZM1 (PG12A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Group XIIA secretory phospholipase A2
Short name=GXII sPLA2
Short name=sPLA2-XII
EC=3.1.1.4

Alternative name(s):
Phosphatidylcholine 2-acylhydrolase 12A

Gene names

Name:	PLA2G12A
Synonyms:	PLA2G12
ORF Names:	FKSG38, UNQ2519/PRO6012

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl-phosphatidylcholine or -phosphatidylethanolamine. Ref.5
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted. Cytoplasm Ref.5.
Tissue specificity	Abundantly expressed in heart, skeletal muscle, kidney, liver and pancreas.
Sequence similarities	Belongs to the phospholipase A2 family.
Mass spectrometry	Molecular mass is 18702.6±0.5 Da from positions 22 - 189. Determined by MALDI. Ref.1

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cytoplasm Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Non-traceable author statement Ref.1. Source: UniProtKB
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent phospholipase A2 activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Probable
	Chain	23 – 189	167	Group XIIA secretory phospholipase A2		PRO_0000022770
Sites
	Active site	110	1	By similarity
	Active site	125	1	By similarity
	Metal binding	88	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	90	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	92	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	111	1	Calcium By similarity
Experimental info
	Sequence conflict	94 – 95	2	Missing in AAG50289. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9BZM1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7979881D6E9223A4	FASTA	189	21,067
	10 20 30 40 50 60 MALLSRPALT LLLLLMAAVV RCQEQAQTTD WRATLKTIRN GVHKIDTYLN AALDLLGGED 70 80 90 100 110 120 GLCQYKCSDG SKPFPRYGYK PSPPNGCGSP LFGVHLNIGI PSLTKCCNQH DRCYETCGKS 130 140 150 160 170 180 KNDCDEEFQY CLSKICRDVQ KTLGLTQHVQ ACETTVELLF DSVIHLGCKP YLDSQRAACR CHYEEKTDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and recombinant expression of a structurally novel human secreted phospholipase A2." Gelb M.H., Valentin E., Ghomashchi F., Lazdunski M., Lambeau G. J. Biol. Chem. 275:39823-39826(2000) [PubMed: 11031251] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, MASS SPECTROMETRY.
[2]	"Identification of FKSG38, a novel gene located on human chromosome 4q25." Wang Y.-G., Gong L. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[5]	"Cellular arachidonate-releasing function of novel classes of secretory phospholipase A2s (groups III and XII)." Murakami M., Masuda S., Shimbara S., Bezzine S., Lazdunski M., Lambeau G., Gelb M.H., Matsukura S., Kokubu F., Adachi M., Kudo I. J. Biol. Chem. 278:10657-10667(2003) [PubMed: 12522102] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF306567 mRNA. Translation: AAG50243.1. AF332892 mRNA. Translation: AAG50289.1. AY359024 mRNA. Translation: AAQ89383.1. BC017218 mRNA. Translation: AAH17218.1.
IPI	IPI00017095.
RefSeq	NP_110448.2. NM_030821.4.
UniGene	Hs.389452.
3D structure databases
ProteinModelPortal	Q9BZM1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BZM1. 9 interactions.
STRING	Q9BZM1.
Polymorphism databases
DMDM	20143881.
Proteomic databases
PeptideAtlas	Q9BZM1.
PRIDE	Q9BZM1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000243501; ENSP00000243501; ENSG00000123739.
GeneID	81579.
KEGG	hsa:81579.
UCSC	uc003hzp.1. human.
Organism-specific databases
CTD	81579.
GeneCards	GC04M110631.
H-InvDB	HIX0004437.
HGNC	HGNC:18554. PLA2G12A.
HPA	HPA035909.
MIM	611652. gene.
neXtProt	NX_Q9BZM1.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00390000008798.
HOGENOM	HBG714426.
HOVERGEN	HBG053577.
InParanoid	Q9BZM1.
OMA	TQHVQAC.
OrthoDB	EOG4MPHR9.
PhylomeDB	Q9BZM1.
Gene expression databases
ArrayExpress	Q9BZM1.
Bgee	Q9BZM1.
CleanEx	HS_PLA2G12A.
Genevestigator	Q9BZM1.
GermOnline	ENSG00000123739. Homo sapiens.
Family and domain databases
InterPro	IPR016090. PLipase_A2. IPR013090. PLipase_A2_AS. IPR010711. PLipase_A2_secretory_G12. [Graphical view]
Gene3D	G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
KO	K01047.
PANTHER	PTHR12824. PLA2G12. 1 hit.
Pfam	PF06951. PLA2G12. 1 hit. [Graphical view]
SUPFAM	SSF48619. PhospholipaseA2. 1 hit.
PROSITE	PS00119. PA2_ASP. False negative. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	71900.
SOURCE	Search...

Entry information

Entry name

PG12A_HUMAN

Accession

Primary (citable) accession number: Q9BZM1
Secondary accession number(s): Q9BZ89

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.