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Q9BZL4

- PP12C_HUMAN

UniProt

Q9BZL4 - PP12C_HUMAN

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Protein

Protein phosphatase 1 regulatory subunit 12C

Gene

PPP1R12C

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulates myosin phosphatase activity.1 Publication

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 12C
Alternative name(s):
Protein phosphatase 1 myosin-binding subunit of 85 kDa
Short name:
Protein phosphatase 1 myosin-binding subunit p85
Gene namesi
Name:PPP1R12CImported
Synonyms:LENG3, MBS851 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:14947. PPP1R12C.

Subcellular locationi

Cytoplasm 2 Publications
Note: Along actomyosin filaments and stress fibers.2 Publications

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33619.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 782781Protein phosphatase 1 regulatory subunit 12CPRO_0000315863Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei560 – 5601Phosphothreonine; by CDC42BP and ROCK21 Publication
Modified residuei604 – 6041Phosphoserine1 Publication

Post-translational modificationi

Phosphorylation at Thr-560 is essential for its interaction with PPP1CB.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9BZL4.
PaxDbiQ9BZL4.
PRIDEiQ9BZL4.

PTM databases

PhosphoSiteiQ9BZL4.

Expressioni

Tissue specificityi

Ubiquitously expressed. Highly expressed in heart.1 Publication

Gene expression databases

BgeeiQ9BZL4.
CleanExiHS_PPP1R12C.
ExpressionAtlasiQ9BZL4. baseline.
GenevestigatoriQ9BZL4.

Organism-specific databases

HPAiHPA043532.

Interactioni

Subunit structurei

PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12C mediates binding to myosin. Interacts via its N-terminus with PPP1CB. Interacts with IL16. Interacts with the coiled-coil domain of MPRIP.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SMAD3P840222EBI-721802,EBI-347161

Protein-protein interaction databases

BioGridi120144. 9 interactions.
IntActiQ9BZL4. 9 interactions.
MINTiMINT-1488862.
STRINGi9606.ENSP00000263433.

Structurei

3D structure databases

ProteinModelPortaliQ9BZL4.
SMRiQ9BZL4. Positions 16-315, 527-578.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati100 – 12930ANK 1Sequence AnalysisAdd
BLAST
Repeati133 – 16230ANK 2Sequence AnalysisAdd
BLAST
Repeati226 – 25530ANK 3Sequence AnalysisAdd
BLAST
Repeati259 – 28830ANK 4Sequence AnalysisAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili297 – 32933Sequence AnalysisAdd
BLAST
Coiled coili681 – 782102Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi473 – 52351Pro-richSequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 4 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00760000119141.
HOVERGENiHBG104929.
InParanoidiQ9BZL4.
KOiK17457.
OMAiPDGGFRK.
OrthoDBiEOG7060Q5.
PhylomeDBiQ9BZL4.
TreeFamiTF105543.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017401. Pase-1_reg_su_12A/B/C_euk.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PIRSFiPIRSF038141. PP1_12ABC_vert. 1 hit.
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 13 Publications (identifier: Q9BZL4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGEDGPAAG PGAAAAAARE RRREQLRQWG ARAGAEPGPG ERRARTVRFE
60 70 80 90 100
RAAEFLAACA GGDLDEARLM LRAADPGPGA ELDPAAPPPA RAVLDSTNAD
110 120 130 140 150
GISALHQACI DENLEVVRFL VEQGATVNQA DNEGWTPLHV AASCGYLDIA
160 170 180 190 200
RYLLSHGANI AAVNSDGDLP LDLAESDAME GLLKAEIARR GVDVEAAKRA
210 220 230 240 250
EEELLLHDTR CWLNGGAMPE ARHPRTGASA LHVAAAKGYI EVMRLLLQAG
260 270 280 290 300
YDPELRDGDG WTPLHAAAHW GVEDACRLLA EHGGGMDSLT HAGQRPCDLA
310 320 330 340 350
DEEVLSLLEE LARKQEDLRN QKEASQSRGQ EPQAPSSSKH RRSSVCRLSS
360 370 380 390 400
REKISLQDLS KERRPGGAGG PPIQDEDEGE EGPTEPPPAE PRTLNGVSSP
410 420 430 440 450
PHPSPKSPVQ LEEAPFSRRF GLLKTGSSGA LGPPERRTAE GAPGAGLQRS
460 470 480 490 500
ASSSWLEGTS TQAKELRLAR ITPTPSPKLP EPSVLSEVTK PPPCLENSSP
510 520 530 540 550
PSRIPEPESP AKPNVPTAST APPADSRDRR RSYQMPVRDE ESESQRKARS
560 570 580 590 600
RLMRQSRRST QGVTLTDLKE AEKAAGKAPE SEKPAQSLDP SRRPRVPGVE
610 620 630 640 650
NSDSPAQRAE APDGQGPGPQ AAREHRKVGK EWRGPAEGEE AEPADRSQES
660 670 680 690 700
STLEGGPSAR RQRWQRDLNP EPEPESEEPD GGFRTLYAEL RRENERLREA
710 720 730 740 750
LTETTLRLAQ LKVELERATQ RQERFAERPA LLELERFERR ALERKAAELE
760 770 780
EELKALSDLR ADNQRLKDEN AALIRVISKL SK
Length:782
Mass (Da):84,881
Last modified:June 1, 2001 - v1
Checksum:i677E7EB1B62C2CC8
GO
Isoform 21 Publication (identifier: Q9BZL4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     576-638: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:719
Mass (Da):78,234
Checksum:i6E800246823EC0C4
GO
Isoform 3 (identifier: Q9BZL4-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     410-410: Missing.
     609-609: Missing.

Note: No experimental confirmation available.

Show »
Length:780
Mass (Da):84,682
Checksum:iCD10D87B6E68FE49
GO
Isoform 4 (identifier: Q9BZL4-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     609-609: Missing.

Note: No experimental confirmation available.

Show »
Length:781
Mass (Da):84,810
Checksum:iEE25D3C5CF1E4D6F
GO

Sequence cautioni

The sequence AF211968 differs from that shown. Reason: Unspliced mRNA.
The sequence BAB15651.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC85179.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti438 – 4381T → I in AAH08030. (PubMed:15489334)Curated
Sequence conflicti527 – 5271R → W in BAB15651. (PubMed:14702039)Curated
Sequence conflicti537 – 5371V → A in BAC85179. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti419 – 4191R → C.
Corresponds to variant rs35849605 [ dbSNP | Ensembl ].
VAR_048310

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei410 – 4101Missing in isoform 3. 2 PublicationsVSP_030750
Alternative sequencei576 – 63863Missing in isoform 2. 1 PublicationVSP_052643Add
BLAST
Alternative sequencei609 – 6091Missing in isoform 3 and isoform 4. 3 PublicationsVSP_030751

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF312028 mRNA. Translation: AAG60045.1.
AB209452 mRNA. Translation: BAD92689.1.
AK027086 mRNA. Translation: BAB15651.1. Different initiation.
AK129529 mRNA. Translation: BAC85179.1. Different initiation.
AF211968 mRNA. No translation available.
AL137618 mRNA. Translation: CAB70844.1.
BC008030 mRNA. Translation: AAH08030.1.
CCDSiCCDS12916.1. [Q9BZL4-1]
PIRiT46318.
RefSeqiNP_001258547.1. NM_001271618.1. [Q9BZL4-3]
NP_060077.1. NM_017607.3. [Q9BZL4-1]
XP_005259070.1. XM_005259013.2. [Q9BZL4-4]
UniGeneiHs.631579.

Genome annotation databases

EnsembliENST00000263433; ENSP00000263433; ENSG00000125503. [Q9BZL4-1]
GeneIDi54776.
KEGGihsa:54776.
UCSCiuc002qix.4. human. [Q9BZL4-1]
uc002qiy.4. human. [Q9BZL4-3]

Polymorphism databases

DMDMi74752476.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF312028 mRNA. Translation: AAG60045.1 .
AB209452 mRNA. Translation: BAD92689.1 .
AK027086 mRNA. Translation: BAB15651.1 . Different initiation.
AK129529 mRNA. Translation: BAC85179.1 . Different initiation.
AF211968 mRNA. No translation available.
AL137618 mRNA. Translation: CAB70844.1 .
BC008030 mRNA. Translation: AAH08030.1 .
CCDSi CCDS12916.1. [Q9BZL4-1 ]
PIRi T46318.
RefSeqi NP_001258547.1. NM_001271618.1. [Q9BZL4-3 ]
NP_060077.1. NM_017607.3. [Q9BZL4-1 ]
XP_005259070.1. XM_005259013.2. [Q9BZL4-4 ]
UniGenei Hs.631579.

3D structure databases

ProteinModelPortali Q9BZL4.
SMRi Q9BZL4. Positions 16-315, 527-578.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120144. 9 interactions.
IntActi Q9BZL4. 9 interactions.
MINTi MINT-1488862.
STRINGi 9606.ENSP00000263433.

PTM databases

PhosphoSitei Q9BZL4.

Polymorphism databases

DMDMi 74752476.

Proteomic databases

MaxQBi Q9BZL4.
PaxDbi Q9BZL4.
PRIDEi Q9BZL4.

Protocols and materials databases

DNASUi 54776.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000263433 ; ENSP00000263433 ; ENSG00000125503 . [Q9BZL4-1 ]
GeneIDi 54776.
KEGGi hsa:54776.
UCSCi uc002qix.4. human. [Q9BZL4-1 ]
uc002qiy.4. human. [Q9BZL4-3 ]

Organism-specific databases

CTDi 54776.
GeneCardsi GC19M055602.
HGNCi HGNC:14947. PPP1R12C.
HPAi HPA043532.
MIMi 613245. gene.
neXtProti NX_Q9BZL4.
PharmGKBi PA33619.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0666.
GeneTreei ENSGT00760000119141.
HOVERGENi HBG104929.
InParanoidi Q9BZL4.
KOi K17457.
OMAi PDGGFRK.
OrthoDBi EOG7060Q5.
PhylomeDBi Q9BZL4.
TreeFami TF105543.

Miscellaneous databases

GenomeRNAii 54776.
NextBioi 57429.
PROi Q9BZL4.
SOURCEi Search...

Gene expression databases

Bgeei Q9BZL4.
CleanExi HS_PPP1R12C.
ExpressionAtlasi Q9BZL4. baseline.
Genevestigatori Q9BZL4.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017401. Pase-1_reg_su_12A/B/C_euk.
[Graphical view ]
Pfami PF12796. Ank_2. 2 hits.
[Graphical view ]
PIRSFi PIRSF038141. PP1_12ABC_vert. 1 hit.
PRINTSi PR01415. ANKYRIN.
SMARTi SM00248. ANK. 4 hits.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Phosphorylation of a novel myosin binding subunit of protein phosphatase 1 reveals a conserved mechanism in the regulation of actin cytoskeleton."
    Tan I., Ng C.H., Lim L., Leung T.
    J. Biol. Chem. 276:21209-21216(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH PPP1CB, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION AT THR-560.
  2. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 45-782 (ISOFORM 3).
    Tissue: BrainImported.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 103-782 (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 487-782 (ISOFORM 2).
    Tissue: Kidney epitheliumImported and PericardiumImported.
  4. "Extensive gene duplications and a large inversion characterize the human leukocyte receptor cluster."
    Wende H., Volz A., Ziegler A.
    Immunogenetics 51:703-713(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 107-191 (ISOFORM 1).
    Tissue: Thymus.
  5. Erratum
    Wende H., Volz A., Ziegler A.
    Immunogenetics 52:3-4(2001)
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 304-782 (ISOFORM 1).
    Tissue: Testis.
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 420-782 (ISOFORM 4).
    Tissue: Endometrial adenocarcinomaImported.
  8. "PDZ domain-mediated interaction of interleukin-16 precursor proteins with myosin phosphatase targeting subunits."
    Bannert N., Vollhardt K., Asomuddinov B., Haag M., Koenig H., Norley S., Kurth R.
    J. Biol. Chem. 278:42190-42199(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IL16, SUBCELLULAR LOCATION.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-604, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  12. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPP12C_HUMAN
AccessioniPrimary (citable) accession number: Q9BZL4
Secondary accession number(s): Q59FK8
, Q6ZPD1, Q7L8F7, Q96HW1, Q9H5H5, Q9NT00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3