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Q9BZL4 (PP12C_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 1 regulatory subunit 12C
Alternative name(s):
Protein phosphatase 1 myosin-binding subunit of 85 kDa
Short name=Protein phosphatase 1 myosin-binding subunit p85
Gene names
Name:PPP1R12C
Synonyms:LENG3, MBS85
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length782 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates myosin phosphatase activity. Ref.1

Subunit structure

PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12C mediates binding to myosin. Interacts via its N-terminus with PPP1CB. Interacts with IL16. Interacts with the coiled-coil domain of MPRIP. Ref.1 Ref.8

Subcellular location

Cytoplasm. Note: Along actomyosin filaments and stress fibers. Ref.1 Ref.8

Tissue specificity

Ubiquitously expressed. Highly expressed in heart. Ref.1

Post-translational modification

Phosphorylation at Thr-560 is essential for its interaction with PPP1CB.

Sequence similarities

Contains 4 ANK repeats.

Caution

Although assigned as two separate genes (PPP1R12C and LENG3), it is probable that LENG3 does not exist by itself and is a part of the PPP1R12C gene.

Sequence caution

The sequence AF211968 differs from that shown. Reason: Unspliced mRNA.

The sequence BAB15651.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC85179.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainANK repeat
Coiled coil
Repeat
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 21988832. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SMAD3P840222EBI-721802,EBI-347161

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 Ref.3 Ref.4 Ref.7 Ref.13 (identifier: Q9BZL4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.3 (identifier: Q9BZL4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     576-638: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q9BZL4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     410-410: Missing.
     609-609: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q9BZL4-4)

The sequence of this isoform differs from the canonical sequence as follows:
     609-609: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.13
Chain2 – 782781Protein phosphatase 1 regulatory subunit 12C
PRO_0000315863

Regions

Repeat100 – 12930ANK 1
Repeat133 – 16230ANK 2
Repeat226 – 25530ANK 3
Repeat259 – 28830ANK 4
Coiled coil297 – 32933 Potential
Coiled coil681 – 782102 Potential
Compositional bias473 – 52351Pro-rich

Amino acid modifications

Modified residue21N-acetylserine Ref.13
Modified residue5601Phosphothreonine; by CDC42BP and ROCK2 Ref.1 Ref.9
Modified residue6041Phosphoserine Ref.10

Natural variations

Alternative sequence4101Missing in isoform 3.
VSP_030750
Alternative sequence576 – 63863Missing in isoform 2. Ref.3
VSP_052643
Alternative sequence6091Missing in isoform 3 and isoform 4.
VSP_030751
Natural variant4191R → C.
Corresponds to variant rs35849605 [ dbSNP | Ensembl ].
VAR_048310

Experimental info

Sequence conflict4381T → I in AAH08030. Ref.7
Sequence conflict5271R → W in BAB15651. Ref.3
Sequence conflict5371V → A in BAC85179. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 677E7EB1B62C2CC8

FASTA78284,881
        10         20         30         40         50         60 
MSGEDGPAAG PGAAAAAARE RRREQLRQWG ARAGAEPGPG ERRARTVRFE RAAEFLAACA 

        70         80         90        100        110        120 
GGDLDEARLM LRAADPGPGA ELDPAAPPPA RAVLDSTNAD GISALHQACI DENLEVVRFL 

       130        140        150        160        170        180 
VEQGATVNQA DNEGWTPLHV AASCGYLDIA RYLLSHGANI AAVNSDGDLP LDLAESDAME 

       190        200        210        220        230        240 
GLLKAEIARR GVDVEAAKRA EEELLLHDTR CWLNGGAMPE ARHPRTGASA LHVAAAKGYI 

       250        260        270        280        290        300 
EVMRLLLQAG YDPELRDGDG WTPLHAAAHW GVEDACRLLA EHGGGMDSLT HAGQRPCDLA 

       310        320        330        340        350        360 
DEEVLSLLEE LARKQEDLRN QKEASQSRGQ EPQAPSSSKH RRSSVCRLSS REKISLQDLS 

       370        380        390        400        410        420 
KERRPGGAGG PPIQDEDEGE EGPTEPPPAE PRTLNGVSSP PHPSPKSPVQ LEEAPFSRRF 

       430        440        450        460        470        480 
GLLKTGSSGA LGPPERRTAE GAPGAGLQRS ASSSWLEGTS TQAKELRLAR ITPTPSPKLP 

       490        500        510        520        530        540 
EPSVLSEVTK PPPCLENSSP PSRIPEPESP AKPNVPTAST APPADSRDRR RSYQMPVRDE 

       550        560        570        580        590        600 
ESESQRKARS RLMRQSRRST QGVTLTDLKE AEKAAGKAPE SEKPAQSLDP SRRPRVPGVE 

       610        620        630        640        650        660 
NSDSPAQRAE APDGQGPGPQ AAREHRKVGK EWRGPAEGEE AEPADRSQES STLEGGPSAR 

       670        680        690        700        710        720 
RQRWQRDLNP EPEPESEEPD GGFRTLYAEL RRENERLREA LTETTLRLAQ LKVELERATQ 

       730        740        750        760        770        780 
RQERFAERPA LLELERFERR ALERKAAELE EELKALSDLR ADNQRLKDEN AALIRVISKL 


SK 

« Hide

Isoform 2 [UniParc].

Checksum: 6E800246823EC0C4
Show »

FASTA71978,234
Isoform 3 [UniParc].

Checksum: CD10D87B6E68FE49
Show »

FASTA78084,682
Isoform 4 [UniParc].

Checksum: EE25D3C5CF1E4D6F
Show »

FASTA78184,810

References

« Hide 'large scale' references
[1]"Phosphorylation of a novel myosin binding subunit of protein phosphatase 1 reveals a conserved mechanism in the regulation of actin cytoskeleton."
Tan I., Ng C.H., Lim L., Leung T.
J. Biol. Chem. 276:21209-21216(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH PPP1CB, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION AT THR-560.
[2]Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 45-782 (ISOFORM 3).
Tissue: Brain.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 103-782 (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 487-782 (ISOFORM 2).
Tissue: Kidney epithelium and Pericardium.
[4]"Extensive gene duplications and a large inversion characterize the human leukocyte receptor cluster."
Wende H., Volz A., Ziegler A.
Immunogenetics 51:703-713(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 107-191 (ISOFORM 1).
Tissue: Thymus.
[5]Erratum
Wende H., Volz A., Ziegler A.
Immunogenetics 52:3-4(2001)
[6]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 304-782 (ISOFORM 1).
Tissue: Testis.
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 420-782 (ISOFORM 4).
Tissue: Endometrial adenocarcinoma.
[8]"PDZ domain-mediated interaction of interleukin-16 precursor proteins with myosin phosphatase targeting subunits."
Bannert N., Vollhardt K., Asomuddinov B., Haag M., Koenig H., Norley S., Kurth R.
J. Biol. Chem. 278:42190-42199(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IL16, SUBCELLULAR LOCATION.
[9]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic kidney.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-604, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[12]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF312028 mRNA. Translation: AAG60045.1.
AB209452 mRNA. Translation: BAD92689.1.
AK027086 mRNA. Translation: BAB15651.1. Different initiation.
AK129529 mRNA. Translation: BAC85179.1. Different initiation.
AF211968 mRNA. No translation available.
AL137618 mRNA. Translation: CAB70844.1.
BC008030 mRNA. Translation: AAH08030.1.
CCDSCCDS12916.1. [Q9BZL4-1]
PIRT46318.
RefSeqNP_001258547.1. NM_001271618.1. [Q9BZL4-3]
NP_060077.1. NM_017607.3. [Q9BZL4-1]
XP_005259070.1. XM_005259013.2. [Q9BZL4-4]
UniGeneHs.631579.

3D structure databases

ProteinModelPortalQ9BZL4.
SMRQ9BZL4. Positions 16-327, 527-578.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120144. 7 interactions.
IntActQ9BZL4. 9 interactions.
MINTMINT-1488862.
STRING9606.ENSP00000263433.

PTM databases

PhosphoSiteQ9BZL4.

Polymorphism databases

DMDM74752476.

Proteomic databases

MaxQBQ9BZL4.
PaxDbQ9BZL4.
PRIDEQ9BZL4.

Protocols and materials databases

DNASU54776.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000263433; ENSP00000263433; ENSG00000125503. [Q9BZL4-1]
GeneID54776.
KEGGhsa:54776.
UCSCuc002qix.4. human. [Q9BZL4-1]
uc002qiy.4. human. [Q9BZL4-3]

Organism-specific databases

CTD54776.
GeneCardsGC19M055602.
HGNCHGNC:14947. PPP1R12C.
HPAHPA043532.
MIM613245. gene.
neXtProtNX_Q9BZL4.
PharmGKBPA33619.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0666.
HOVERGENHBG104929.
InParanoidQ9BZL4.
KOK17457.
OMAPDGGFRK.
OrthoDBEOG7060Q5.
PhylomeDBQ9BZL4.
TreeFamTF105543.

Gene expression databases

ArrayExpressQ9BZL4.
BgeeQ9BZL4.
CleanExHS_PPP1R12C.
GenevestigatorQ9BZL4.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR017401. Pase-1_reg_su_12A/B/C_euk.
[Graphical view]
PfamPF12796. Ank_2. 2 hits.
[Graphical view]
PIRSFPIRSF038141. PP1_12ABC_vert. 1 hit.
PRINTSPR01415. ANKYRIN.
SMARTSM00248. ANK. 4 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi54776.
NextBio57429.
PROQ9BZL4.
SOURCESearch...

Entry information

Entry namePP12C_HUMAN
AccessionPrimary (citable) accession number: Q9BZL4
Secondary accession number(s): Q59FK8 expand/collapse secondary AC list , Q6ZPD1, Q7L8F7, Q96HW1, Q9H5H5, Q9NT00
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM