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Protein

Tryptase delta

Gene

TPSD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.By similarity

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei81Charge relay systemBy similarity1
Active sitei128Charge relay systemBy similarity1
Active sitei231Charge relay systemBy similarity1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: InterPro
  • serine-type peptidase activity Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciZFISH:HS01837-MONOMER.

Protein family/group databases

MEROPSiS01.054.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptase delta (EC:3.4.21.59)
Alternative name(s):
Delta-tryptase
HmMCP-3-like tryptase III
Mast cell mMCP-7-like
Tryptase-3
Gene namesi
Name:TPSD1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:14118. TPSD1.

Subcellular locationi

  • Secreted By similarity

  • Note: Released from the secretory granules upon mast cell activation.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi23430.
OpenTargetsiENSG00000095917.
PharmGKBiPA37845.

Chemistry databases

ChEMBLiCHEMBL2095193.

Polymorphism and mutation databases

BioMutaiTPSD1.
DMDMi239938722.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
PropeptideiPRO_000002748326 – 37Activation peptideBy similarityAdd BLAST12
ChainiPRO_000002748438 – 242Tryptase deltaAdd BLAST205

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi66 ↔ 82PROSITE-ProRule annotation
Glycosylationi139N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi162 ↔ 237PROSITE-ProRule annotation
Disulfide bondi195 ↔ 218PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9BZJ3.
PeptideAtlasiQ9BZJ3.
PRIDEiQ9BZJ3.

PTM databases

iPTMnetiQ9BZJ3.
PhosphoSitePlusiQ9BZJ3.

Expressioni

Tissue specificityi

Expressed in colon, lung, heart and synovial tissue. May be specific to mast cells.1 Publication

Gene expression databases

BgeeiENSG00000095917.
CleanExiHS_TPSD1.
ExpressionAtlasiQ9BZJ3. baseline and differential.
GenevisibleiQ9BZJ3. HS.

Organism-specific databases

HPAiCAB002215.
CAB032871.
HPA040182.
HPA049554.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9606.ENSP00000211076.

Structurei

3D structure databases

ProteinModelPortaliQ9BZJ3.
SMRiQ9BZJ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 242Peptidase S1PROSITE-ProRule annotationAdd BLAST205

Sequence similaritiesi

Belongs to the peptidase S1 family. Tryptase subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118810.
HOVERGENiHBG013304.
InParanoidiQ9BZJ3.
KOiK01340.
OMAiWINENVF.
OrthoDBiEOG091G0AH5.
PhylomeDBiQ9BZJ3.
TreeFamiTF351676.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BZJ3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLLAPQMLS LLLLALPVLA SPAYVAPAPG QALQQTGIVG GQEAPRSKWP
60 70 80 90 100
WQVSLRVRGP YWMHFCGGSL IHPQWVLTAA HCVEPDIKDL AALRVQLREQ
110 120 130 140 150
HLYYQDQLLP VSRIIVHPQF YIIQTGADIA LLELEEPVNI SSHIHTVTLP
160 170 180 190 200
PASETFPPGM PCWVTGWGDV DNNVHLPPPY PLKEVEVPVV ENHLCNAEYH
210 220 230 240
TGLHTGHSFQ IVRDDMLCAG SENHDSCQGD SGGPLVCKVN GT
Length:242
Mass (Da):26,584
Last modified:June 16, 2009 - v2
Checksum:i6E72D8AE1EDEA2F1
GO
Isoform 2 (identifier: Q9BZJ3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     86-94: Missing.

Show »
Length:233
Mass (Da):25,587
Checksum:i74A9069BFC39CF44
GO

Sequence cautioni

The sequence AAD17861 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAK12909 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25V → G in AAD17861 (PubMed:9920877).Curated1
Sequence conflicti178P → S in AAL17874 (PubMed:12391231).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01687022P → R.1 PublicationCorresponds to variant rs3865205dbSNPEnsembl.1
Natural variantiVAR_01687125V → A.Corresponds to variant rs1800984dbSNPEnsembl.1
Natural variantiVAR_01687283V → M.3 PublicationsCorresponds to variant rs3993987dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00831986 – 94Missing in isoform 2. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098327 Genomic DNA. Translation: AAD17845.1.
AF099147 Genomic DNA. Translation: AAD17861.1. Different initiation.
AF318074 Genomic DNA. Translation: AAK12909.1. Different initiation.
AE006466 Genomic DNA. Translation: AAK61272.1.
AC120498 Genomic DNA. No translation available.
AF421357 mRNA. Translation: AAL86695.1.
AF206664 mRNA. Translation: AAG35694.1.
AY055427 mRNA. Translation: AAL17874.1.
CCDSiCCDS10432.1. [Q9BZJ3-1]
RefSeqiNP_036349.1. NM_012217.2. [Q9BZJ3-1]
UniGeneiHs.677789.

Genome annotation databases

EnsembliENST00000211076; ENSP00000211076; ENSG00000095917. [Q9BZJ3-1]
GeneIDi23430.
KEGGihsa:23430.
UCSCiuc002clb.2. human. [Q9BZJ3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098327 Genomic DNA. Translation: AAD17845.1.
AF099147 Genomic DNA. Translation: AAD17861.1. Different initiation.
AF318074 Genomic DNA. Translation: AAK12909.1. Different initiation.
AE006466 Genomic DNA. Translation: AAK61272.1.
AC120498 Genomic DNA. No translation available.
AF421357 mRNA. Translation: AAL86695.1.
AF206664 mRNA. Translation: AAG35694.1.
AY055427 mRNA. Translation: AAL17874.1.
CCDSiCCDS10432.1. [Q9BZJ3-1]
RefSeqiNP_036349.1. NM_012217.2. [Q9BZJ3-1]
UniGeneiHs.677789.

3D structure databases

ProteinModelPortaliQ9BZJ3.
SMRiQ9BZJ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000211076.

Chemistry databases

ChEMBLiCHEMBL2095193.

Protein family/group databases

MEROPSiS01.054.

PTM databases

iPTMnetiQ9BZJ3.
PhosphoSitePlusiQ9BZJ3.

Polymorphism and mutation databases

BioMutaiTPSD1.
DMDMi239938722.

Proteomic databases

PaxDbiQ9BZJ3.
PeptideAtlasiQ9BZJ3.
PRIDEiQ9BZJ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000211076; ENSP00000211076; ENSG00000095917. [Q9BZJ3-1]
GeneIDi23430.
KEGGihsa:23430.
UCSCiuc002clb.2. human. [Q9BZJ3-1]

Organism-specific databases

CTDi23430.
DisGeNETi23430.
GeneCardsiTPSD1.
H-InvDBHIX0038551.
HGNCiHGNC:14118. TPSD1.
HPAiCAB002215.
CAB032871.
HPA040182.
HPA049554.
MIMi609272. gene.
neXtProtiNX_Q9BZJ3.
OpenTargetsiENSG00000095917.
PharmGKBiPA37845.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118810.
HOVERGENiHBG013304.
InParanoidiQ9BZJ3.
KOiK01340.
OMAiWINENVF.
OrthoDBiEOG091G0AH5.
PhylomeDBiQ9BZJ3.
TreeFamiTF351676.

Enzyme and pathway databases

BioCyciZFISH:HS01837-MONOMER.

Miscellaneous databases

GeneWikiiTPSD1.
GenomeRNAii23430.
PROiQ9BZJ3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000095917.
CleanExiHS_TPSD1.
ExpressionAtlasiQ9BZJ3. baseline and differential.
GenevisibleiQ9BZJ3. HS.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRYD_HUMAN
AccessioniPrimary (citable) accession number: Q9BZJ3
Secondary accession number(s): O95824
, Q8TDI6, Q96L36, Q96RZ5, Q9H2Y6, Q9UQI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: June 16, 2009
Last modified: November 2, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Although PubMed:11174199 reported this as a pseudogene, PubMed:12391231 showed it is expressed and has proteolytic activity when expressed in bacterial cells.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.