Reviewed,
UniProtKB/Swiss-Prot Q9BZJ3 (TRYD_HUMAN)
Last modified
July 7, 2009.
Version 69.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Tryptase delta EC=3.4.21.59 Alternative name(s): Delta-tryptase Mast cell mMCP-7-like Tryptase-3 HmMCP-3-like tryptase III | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) [Complete proteome] | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 242 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type By similarity. |
| Catalytic activity | Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin. Ref.6 |
| Subunit structure | Homotetramer By similarity. |
| Subcellular location | Secreted By similarity. Note: Released from the secretory granules upon mast cell activation By similarity. |
| Tissue specificity | Expressed in colon, lung, heart and synovial tissue. May be specific to mast cells. Ref.6 |
| Sequence similarities | Belongs to the peptidase S1 family. Tryptase subfamily. Contains 1 peptidase S1 domain. |
| Caution | Although Ref.2 reported this as a pseudogene, Ref.6 showed it is expressed and has proteolytic activity when expressed in bacterial cells. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Secreted |
| Coding sequence diversity | Alternative splicing Polymorphism |
| Domain | Signal |
| Molecular function | Hydrolase Protease Serine protease |
| PTM | Disulfide bond Glycoprotein Zymogen |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | proteolysis Inferred from electronic annotation. Source: InterPro |
| Cellular component | extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q9BZJ3-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q9BZJ3-2) The sequence of this isoform differs from the canonical sequence as follows: 86-94: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 25 | 25 | Potential | ||||||||
| Propeptide | 26 – 37 | 12 | Activation peptide By similarity | PRO_0000027483 | |||||||
| Chain | 38 – 242 | 205 | Tryptase delta | PRO_0000027484 | |||||||
Regions | |||||||||||
| Domain | 38 – 242 | 205 | Peptidase S1 | ||||||||
Sites | |||||||||||
| Active site | 81 | 1 | Charge relay system By similarity | ||||||||
| Active site | 128 | 1 | Charge relay system By similarity | ||||||||
| Active site | 231 | 1 | Charge relay system By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 139 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 66 ↔ 82 | By similarity | |||||||||
| Disulfide bond | 162 ↔ 237 | By similarity | |||||||||
| Disulfide bond | 195 ↔ 218 | By similarity | |||||||||
Natural variations | |||||||||||
| Alternative sequence | 86 – 94 | 9 | Missing in isoform 2. | VSP_008319 | |||||||
| Natural variant | 22 | 1 | P → R: dbSNP rs3865205. | VAR_016870 | |||||||
| Natural variant | 25 | 1 | V → A: dbSNP rs1800984. | VAR_016871 | |||||||
| Natural variant | 83 | 1 | V → M: dbSNP rs3993987. | VAR_016872 | |||||||
Experimental info | |||||||||||
| Sequence conflict | 25 | 1 | V → G in AAD17861. Ref.1 | ||||||||
| Sequence conflict | 178 | 1 | P → S in AAL17874. Ref.6 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Characterization of genes encoding known and novel human mast cell tryptases on chromosome 16p13.3." Pallaoro M., Fejzo M.S., Shayesteh L., Blount J.L., Caughey G.H. J. Biol. Chem. 274:3355-3362(1999) [PubMed: 9920877] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ARG-22 AND MET-83. |
| [2] | "Human mouse mast cell protease 7-like tryptase genes are pseudogenes." Min H.-K., Kambe N., Schwartz L.B. J. Allergy Clin. Immunol. 107:315-321(2001) [PubMed: 11174199] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16." Daniels R.J., Peden J.F., Lloyd C., Horsley S.W., Clark K., Tufarelli C., Kearney L., Buckle V.J., Doggett N.A., Flint J., Higgs D.R. Hum. Mol. Genet. 10:339-352(2001) [PubMed: 11157797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. Pennacchio L.A.Nature 432:988-994(2004) [PubMed: 15616553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | "Alternate mRNA splicing in multiple human tryptase genes is predicted to regulate tetramer formation." Jackson N.E., Wang H.W., Bryant K.J., McNeil H.P., Husain A., Liu K., Tedla N., Thomas P.S., King G.C., Hettiaratchi A., Cairns J., Hunt J.E. J. Biol. Chem. 283:34178-34187(2008) [PubMed: 18854315] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 40-178 (ISOFORM 2), VARIANT MET-83. |
| [6] | "Delta tryptase is expressed in multiple human tissues, and a recombinant form has proteolytic activity." Wang H.-W., McNeil H.P., Husain A., Liu K., Tedla N., Thomas P.S., Raftery M., King G.C., Cai Z.Y., Hunt J.E. J. Immunol. 169:5145-5152(2002) [PubMed: 12391231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-242 (ISOFORM 1), ENZYME ACTIVITY, TISSUE SPECIFICITY, VARIANT MET-83. |
Cross-references
Sequence databases | |
|---|---|
| AF098327 Genomic DNA. Translation: AAD17845.1. AF099147 Genomic DNA. Translation: AAD17861.1. Different initiation. AF318074 Genomic DNA. Translation: AAK12909.1. Different initiation. AE006466 Genomic DNA. Translation: AAK61272.1. AC120498 Genomic DNA. No translation available. AF421357 mRNA. Translation: AAL86695.1. AF206664 mRNA. Translation: AAG35694.1. AY055427 mRNA. Translation: AAL17874.1. | |
| IPI | IPI00376200. IPI00419573. |
| UniGene | Hs.677789 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1A0L based on UniProtKB P20231. |
| SMR | Q9BZJ3. Positions 31-235. |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | S01.054. |
PTM databases | |
| PhosphoSite | Q9BZJ3. |
Proteomic databases | |
| PRIDE | Q9BZJ3. |
Genome annotation databases | |
| Ensembl | ENSG00000095917. Homo sapiens. [Contig view] |
| UCSC | uc002clb.1. human. uc010brm.1. human. |
Organism-specific databases | |
| GeneCards | GC16P001246. GC16P001247. |
| H-InvDB | HIX0029969. |
| HGNC | HGNC:14118. TPSD1. |
| HPA | CAB002215. |
| MIM | 609272. gene. |
| PharmGKB | PA37845. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOVERGEN | Q9BZJ3. |
Enzyme and pathway databases | |
| BRENDA | 3.4.21.59. 247. |
Gene expression databases | |
| ArrayExpress | Q9BZJ3. |
| Bgee | Q9BZJ3. |
| CleanEx | HS_TPSD1. |
| GermOnline | ENSG00000095917. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view] |
| Pfam | PF00089. Trypsin. 1 hit. [Graphical view] |
| PRINTS | PR00722. CHYMOTRYPSIN. |
| SMART | SM00020. Tryp_SPc. 1 hit. [Graphical view] |
| PROSITE | PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| SOURCE | Search... |
Entry information
| Entry name | TRYD_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9BZJ3 Secondary accession number(s): O95824 Q9UQI8 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 16 Human chromosome 16: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


