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Q9BZJ3 (TRYD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptase delta

EC=3.4.21.59
Alternative name(s):
Delta-tryptase
HmMCP-3-like tryptase III
Mast cell mMCP-7-like
Tryptase-3
Gene names
Name:TPSD1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length242 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type By similarity.

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin. Ref.6

Subunit structure

Homotetramer By similarity.

Subcellular location

Secreted By similarity. Note: Released from the secretory granules upon mast cell activation By similarity.

Tissue specificity

Expressed in colon, lung, heart and synovial tissue. May be specific to mast cells. Ref.6

Sequence similarities

Belongs to the peptidase S1 family. Tryptase subfamily.

Contains 1 peptidase S1 domain.

Caution

Although Ref.2 reported this as a pseudogene, Ref.6 showed it is expressed and has proteolytic activity when expressed in bacterial cells.

Sequence caution

The sequence AAD17861.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAK12909.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

serine-type peptidase activity

Traceable author statement Ref.1. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9BZJ3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9BZJ3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     86-94: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Propeptide26 – 3712Activation peptide By similarity
PRO_0000027483
Chain38 – 242205Tryptase delta
PRO_0000027484

Regions

Domain38 – 242205Peptidase S1

Sites

Active site811Charge relay system By similarity
Active site1281Charge relay system By similarity
Active site2311Charge relay system By similarity

Amino acid modifications

Glycosylation1391N-linked (GlcNAc...) Potential
Disulfide bond66 ↔ 82 By similarity
Disulfide bond162 ↔ 237 By similarity
Disulfide bond195 ↔ 218 By similarity

Natural variations

Alternative sequence86 – 949Missing in isoform 2.
VSP_008319
Natural variant221P → R. Ref.1
Corresponds to variant rs3865205 [ dbSNP | Ensembl ].
VAR_016870
Natural variant251V → A.
Corresponds to variant rs1800984 [ dbSNP | Ensembl ].
VAR_016871
Natural variant831V → M. Ref.1 Ref.5 Ref.6
Corresponds to variant rs3993987 [ dbSNP | Ensembl ].
VAR_016872

Experimental info

Sequence conflict251V → G in AAD17861. Ref.1
Sequence conflict1781P → S in AAL17874. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 16, 2009. Version 2.
Checksum: 6E72D8AE1EDEA2F1

FASTA24226,584
        10         20         30         40         50         60 
MLLLAPQMLS LLLLALPVLA SPAYVAPAPG QALQQTGIVG GQEAPRSKWP WQVSLRVRGP 

        70         80         90        100        110        120 
YWMHFCGGSL IHPQWVLTAA HCVEPDIKDL AALRVQLREQ HLYYQDQLLP VSRIIVHPQF 

       130        140        150        160        170        180 
YIIQTGADIA LLELEEPVNI SSHIHTVTLP PASETFPPGM PCWVTGWGDV DNNVHLPPPY 

       190        200        210        220        230        240 
PLKEVEVPVV ENHLCNAEYH TGLHTGHSFQ IVRDDMLCAG SENHDSCQGD SGGPLVCKVN 


GT 

« Hide

Isoform 2 [UniParc].

Checksum: 74A9069BFC39CF44
Show »

FASTA23325,587

References

« Hide 'large scale' references
[1]"Characterization of genes encoding known and novel human mast cell tryptases on chromosome 16p13.3."
Pallaoro M., Fejzo M.S., Shayesteh L., Blount J.L., Caughey G.H.
J. Biol. Chem. 274:3355-3362(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ARG-22 AND MET-83.
[2]"Human mouse mast cell protease 7-like tryptase genes are pseudogenes."
Min H.-K., Kambe N., Schwartz L.B.
J. Allergy Clin. Immunol. 107:315-321(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence, structure and pathology of the fully annotated terminal 2 Mb of the short arm of human chromosome 16."
Daniels R.J., Peden J.F., Lloyd C., Horsley S.W., Clark K., Tufarelli C., Kearney L., Buckle V.J., Doggett N.A., Flint J., Higgs D.R.
Hum. Mol. Genet. 10:339-352(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The sequence and analysis of duplication-rich human chromosome 16."
Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. expand/collapse author list , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Alternate mRNA splicing in multiple human tryptase genes is predicted to regulate tetramer formation."
Jackson N.E., Wang H.W., Bryant K.J., McNeil H.P., Husain A., Liu K., Tedla N., Thomas P.S., King G.C., Hettiaratchi A., Cairns J., Hunt J.E.
J. Biol. Chem. 283:34178-34187(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 40-178 (ISOFORM 2), VARIANT MET-83.
[6]"Delta tryptase is expressed in multiple human tissues, and a recombinant form has proteolytic activity."
Wang H.-W., McNeil H.P., Husain A., Liu K., Tedla N., Thomas P.S., Raftery M., King G.C., Cai Z.Y., Hunt J.E.
J. Immunol. 169:5145-5152(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 53-242 (ISOFORM 1), ENZYME ACTIVITY, TISSUE SPECIFICITY, VARIANT MET-83.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF098327 Genomic DNA. Translation: AAD17845.1.
AF099147 Genomic DNA. Translation: AAD17861.1. Different initiation.
AF318074 Genomic DNA. Translation: AAK12909.1. Different initiation.
AE006466 Genomic DNA. Translation: AAK61272.1.
AC120498 Genomic DNA. No translation available.
AF421357 mRNA. Translation: AAL86695.1.
AF206664 mRNA. Translation: AAG35694.1.
AY055427 mRNA. Translation: AAL17874.1.
IPIIPI00376200.
IPI00419573.
RefSeqNP_036349.1. NM_012217.2.
UniGeneHs.677789.

3D structure databases

ProteinModelPortalQ9BZJ3.
ModBaseSearch...

Protein family/group databases

MEROPSS01.054.

PTM databases

PhosphoSiteQ9BZJ3.

Polymorphism databases

DMDM239938722.

Proteomic databases

PaxDbQ9BZJ3.
PRIDEQ9BZJ3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000211076; ENSP00000211076; ENSG00000095917.
ENST00000397534; ENSP00000380668; ENSG00000095917.
GeneID23430.
KEGGhsa:23430.
UCSCuc002clb.1. human.

Organism-specific databases

CTD23430.
GeneCardsGC16P001307.
H-InvDBHIX0038551.
HGNCHGNC:14118. TPSD1.
HPACAB002215.
MIM609272. gene.
neXtProtNX_Q9BZJ3.
PharmGKBPA37845.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5640.
HOVERGENHBG013304.
InParanoidQ9BZJ3.
KOK01340.
OMASSHIHTV.
PhylomeDBQ9BZJ3.

Gene expression databases

BgeeQ9BZJ3.
CleanExHS_TPSD1.
GenevestigatorQ9BZJ3.
GermOnlineENSG00000095917. Homo sapiens.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. Pept_Ser_Cys. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBQ9BZJ3.
ChEMBLCHEMBL2402.
GenomeRNAi23430.
NextBio45673.
SOURCESearch...

Entry information

Entry nameTRYD_HUMAN
AccessionPrimary (citable) accession number: Q9BZJ3
Secondary accession number(s): O95824 expand/collapse secondary AC list , Q8TDI6, Q96L36, Q96RZ5, Q9H2Y6, Q9UQI8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: June 16, 2009
Last modified: May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families