Q9BZG9 (LYNX1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ly-6/neurotoxin-like protein 1

Alternative name(s):
Secreted Ly-6/uPAR domain-containing protein 2
Secreted Ly-6/uPAR-related protein 2
Short name=SLURP-2

Gene names

Name:	LYNX1
Synonyms:	SLURP2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts in different tissues through interaction to nicotinic acetylcholine receptors (nAChRs). In brain, isoform 2 modulates functional properties of nAChRs to prevent excessive excitation, and hence neurodegeneration. Enhances desensitization by increasing both the rate and extent of desensitization of alpha4beta2 nAChRs and slowing recovery from desensitization. Promotes large amplitude ACh-evoked currents through alpha4beta2 nAChRs By similarity. Prevents plasticity in the primary visual cortex late in life By similarity. In keratinocytes, isoform 3 delays differentiation and prevents apoptosis. Ref.2
Subunit structure	Isoform 2 interacts with nAChRs, including alpha4beta2 (CHRNA4/CHRNB2) and alpha7 (CHRNA7) By similarity. Competes with alpha-bungarotoxin for nAChR alpha7 binding. Isoform 3 may interact with heteropentameric nAChRs expressed by keratinocytes. Ref.2
Subcellular location	Isoform 1: Secreted Potential Ref.2. Isoform 2: Cell membrane; Lipid-anchor › GPI-anchor Potential. Cell projection › dendrite By similarity. Note: Detected in Purkinje cells soma and proximal dendrites By similarity. Ref.2 Isoform 3: Secreted Ref.2.
Tissue specificity	Isoform 3 is expressed at highest levels in cervix and esophagus, followed by adult and fetal skin. Expressed at lower levels in brain, lung, stomach, small intestine, colon, rectum, uterus, and thymus. Not detected in spleen nor bone marrow. In the epidermis, predominantly produced by keratinocytes of the suprabasal epidermal compartment (at protein level). In attached gingiva, produced at highest levels by basal cells located in the lowermost epithelial layers (at protein level). Up-regulated 3-fold in psoriatic lesional skin. Detected in serum (at protein level). Ref.1 Ref.2
Miscellaneous	Isoform 2 is considered to be an endogenous "prototoxin", that shares a N-terminal three-finger structure with snake alpha-neurotoxins.
Sequence similarities	Contains 1 UPAR/Ly6 domain.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW dendrite Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BZG9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BZG9-2)

Also known as: LYNX1;

The sequence of this isoform differs from the canonical sequence as follows:
     52-131: SAAEAIWCHQ...CCQTSLCNHD → YYTPTRMKVS...ILLATLWGLL

Note: Contains a potential GPI-anchor site at Gly-93. The propeptide 94-116 may be cleaved out upon GPI-anchor attachment (Potential).

Isoform 3 (identifier: Q9BZG9-3)

Also known as: SLURP-2;

The sequence of this isoform differs from the canonical sequence as follows:
     2-52: TPLLTLILVVLMGLPLAQALDCHVCAYNGDNCFNPMRCPAMVAYCMTTRTS → QLGTGLLLAAVLSLQLA

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 20

20

Potential

Chain

21 – 131

111

Ly-6/neurotoxin-like protein 1

PRO_0000036154

Regions

Domain

59 – 129

71

UPAR/Ly6

Amino acid modifications

Disulfide bond

59 ↔ 81

Potential

Disulfide bond

62 ↔ 68

Potential

Disulfide bond

74 ↔ 102

Potential

Disulfide bond

106 ↔ 122

Potential

Disulfide bond

123 ↔ 128

Potential

Natural variations

Alternative sequence

2 – 52

51

TPLLT…TTRTS → QLGTGLLLAAVLSLQLA in isoform 3.

VSP_044157

Alternative sequence

52 – 131

80

SAAEA…LCNHD → YYTPTRMKVSKSCVPRCFET VYDGYSKHASTTSCCQYDLC NGTGLATPATLALAPILLAT LWGLL in isoform 2.

VSP_044158

Secondary structure

1

.

.

.

.

.

.

.

.

.

131

Helix Strand Turn

Details...

Beta strand

22 – 24

3

Beta strand

26 – 31

6

Beta strand

36 – 38

3

Beta strand

45 – 51

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 3, 2012. Version 3. Checksum: FAD1259D48A036D7	FASTA	131	14,026
	10 20 30 40 50 60 MTPLLTLILV VLMGLPLAQA LDCHVCAYNG DNCFNPMRCP AMVAYCMTTR TSAAEAIWCH 70 80 90 100 110 120 QCTGFGGCSH GSRCLRDSTH CVTTATRVLS NTEDLPLVTK MCHIGCPDIP SLGLGPYVSI 130 ACCQTSLCNH D
	Isoform 2 (LYNX1) [UniParc]. Checksum: BF5827EB64BFE6F6	FASTA	116	12,641
	10 20 30 40 50 60 MTPLLTLILV VLMGLPLAQA LDCHVCAYNG DNCFNPMRCP AMVAYCMTTR TYYTPTRMKV 70 80 90 100 110 SKSCVPRCFE TVYDGYSKHA STTSCCQYDL CNGTGLATPA TLALAPILLA TLWGLL
	Isoform 3 (SLURP-2) [UniParc]. Checksum: 81719A103EC3001A	FASTA	97	10,160
	10 20 30 40 50 60 MQLGTGLLLA AVLSLQLAAA EAIWCHQCTG FGGCSHGSRC LRDSTHCVTT ATRVLSNTED 70 80 90 LPLVTKMCHI GCPDIPSLGL GPYVSIACCQ TSLCNHD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SLURP-2, a novel member of the human Ly-6 superfamily that is up-regulated in psoriasis vulgaris." Tsuji H., Okamoto K., Matsuzaka Y., Iizuka H., Tamiya G., Inoko H. Genomics 81:26-33(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY. Tissue: Skin.
[2]	"SLURP-2: a novel cholinergic signaling peptide in human mucocutaneous epithelium." Arredondo J., Chernyavsky A.I., Jolkovsky D.L., Webber R.J., Grando S.A. J. Cell. Physiol. 208:238-245(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, INTERACTION WITH HETEROPENTAMERIC NACHRS, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[4]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	The MGC Project Team Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Pancreas.
[7]	Southan C., Simms M., Narjis S. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-80 (ISOFORM 2).
[8]	"NMR structure and action on nicotinic acetylcholine receptors of water-soluble domain of human LYNX1." Lyukmanova E.N., Shenkarev Z.O., Shulepko M.A., Mineev K.S., D'Hoedt D., Kasheverov I.E., Filkin S.Y., Krivolapova A.P., Janickova H., Dolezal V., Dolgikh D.A., Arseniev A.S., Bertrand D., Tsetlin V.I., Kirpichnikov M.P. J. Biol. Chem. 286:10618-10627(2011) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 21-93 (ISOFORM 2).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB081838 mRNA. Translation: BAC56859.1.
AY587277 mRNA. Translation: AAT00512.1.
AY358417 mRNA. Translation: AAQ88783.1.
AC083841 Genomic DNA. No translation available.
CH471162 Genomic DNA. Translation: EAW82299.1.
CH471162 Genomic DNA. Translation: EAW82301.1.
CH471162 Genomic DNA. Translation: EAW82302.1.
CH471162 Genomic DNA. Translation: EAW82303.1.
CH471162 Genomic DNA. Translation: EAW82304.1.
CH471162 Genomic DNA. Translation: EAW82306.1.
BC032306 mRNA. No translation available.
BQ685632 mRNA. No translation available.
AF321824 mRNA. Translation: AAK01642.1.

IPI

IPI00289058.

RefSeq

NP_076435.1. NM_023946.2.
NP_803252.1. NM_177457.3.
NP_803253.1. NM_177458.1.
NP_803429.1. NM_177476.2.
NP_803430.1. NM_177477.2.

UniGene

Hs.158665.
Hs.604828.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VYE	model	-	A	1-80	[»]
2L03	NMR	-	A	21-93	[»]

ProteinModelPortal

Q9BZG9.

SMR

Q9BZG9. Positions 21-47.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000337950.

Protein family/group databases

TCDB

8.A.31.1.1. ly-6 neurotoxin-like Protein1 precursor (Lynx1) family.

Polymorphism databases

DMDM

47117907.

Proteomic databases

PaxDb

Q9BZG9.

PeptideAtlas

Q9BZG9.

PRIDE

Q9BZG9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000317543; ENSP00000319846; ENSG00000180155.
ENST00000335822; ENSP00000337950; ENSG00000180155.
ENST00000345173; ENSP00000332495; ENSG00000180155.
ENST00000395192; ENSP00000378618; ENSG00000180155.
ENST00000398906; ENSP00000381878; ENSG00000180155.

GeneID

66004.

KEGG

hsa:66004.

UCSC

uc003yxa.3. human.
uc003yxc.1. human.

Organism-specific databases

CTD

66004.

GeneCards

GC08M143844.

HGNC

HGNC:29604. LYNX1.

HPA

HPA023711.

MIM

606110. gene.

neXtProt

NX_Q9BZG9.

PharmGKB

PA134874147.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG45102.

HOGENOM

HOG000035114.

HOVERGEN

HBG052377.

OMA

RTYYTPY.

PhylomeDB

Q9BZG9.

Gene expression databases

ArrayExpress

Q9BZG9.

Bgee

Q9BZG9.

CleanEx

HS_LYNX1.

Genevestigator

Q9BZG9.

GermOnline

ENSG00000180155. Homo sapiens.

Family and domain databases

InterPro

IPR001526. LY6_UPAR.
[Graphical view]

Pfam

PF00021. UPAR_LY6. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9BZG9.

GenomeRNAi

66004.

NextBio

67514.

SOURCE

Search...

Entry information

Entry name

LYNX1_HUMAN

Accession

Primary (citable) accession number: Q9BZG9
Secondary accession number(s): D3DWI7, G3XAC2, Q86SR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 3, 2012
Last modified:	May 29, 2013
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.