Reviewed, UniProtKB/Swiss-Prot Q9BZG2 (PPAT_HUMAN)

Names and origin

Protein names

Recommended name:
    Testicular acid phosphatase
    EC=3.1.3.2

Gene names

Name:

ACPT

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Dephosphorylates receptor tyrosine-protein kinase erbB-4 and inhibits the ligand-induced proteolytic cleavage. Ref.2
Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Subunit structure	Homodimer Probable.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Tissue specificity	Expressed mainly in the testis. Also expressed in the brain where they are enriched at the post-synaptic sites. Expressed at lower levels in the trachea, prostate, bone marrow, spinal cord, colon, fetal brain, heart, thymus, fetal liver, spleen, leukocytes, ovary, small intestine, pancreas and skeletal muscle. Expression is significantly lower in testicular cancer tissues than in normal testicular tissues. Isoform 3 is expressed in the testis, trachea, prostate and bone marrow. Ref.1
Induction	Up-regulated by mibolerone (a synthetic androgen) and dihydrotestosterone (DHT) and is down-regulated by estrogen and progestin. Ref.1
Post-translational modification	Glycosylated Probable.
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BZG2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BZG2-2)

Also known as: Variant 3;

The sequence of this isoform differs from the canonical sequence as follows:
     91-183: Missing.

Isoform 3 (identifier: Q9BZG2-3)

Also known as: Truncated variant 1; Truncated variant 2;

The sequence of this isoform differs from the canonical sequence as follows:
     39-92: FRHGDRAPLA...RFLRSRYEAF → RRPHPGLPLA...PPCGHEAWAS
     93-426: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 426	400	Testicular acid phosphatase		PRO_0000259643
Regions
	Topological domain	27 – 393	367	Extracellular Potential
	Transmembrane	394 – 414	21	Potential
	Topological domain	415 – 426	12	Cytoplasmic Potential
Sites
	Active site	41	1	Nucleophile By similarity
	Active site	289	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
	Glycosylation	269	1	N-linked (GlcNAc...) Potential
	Glycosylation	330	1	N-linked (GlcNAc...) Potential
	Glycosylation	339	1	N-linked (GlcNAc...) Potential
	Disulfide bond	159 ↔ 378		By similarity
	Disulfide bond	214 ↔ 312		By similarity
	Disulfide bond	353 ↔ 357		By similarity
Natural variations
	Alternative sequence	39 – 92	54	FRHGD…RYEAF → RRPHPGLPLAPPGLALTSPV PRYSAMATGPRWPPTPWTHT RRWPPPCGHEAWAS in isoform 3.		VSP_021493
	Alternative sequence	91 – 183	93	Missing in isoform 2.		VSP_021494
	Alternative sequence	93 – 426	334	Missing in isoform 3.		VSP_021495

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: BE930398041DB061	FASTA	426	46,090
	10 20 30 40 50 60 MAGLGFWGHP AGPLLLLLLL VLPPRALPEG PLVFVALVFR HGDRAPLASY PMDPHKEVAS 70 80 90 100 110 120 TLWPRGLGQL TTEGVRQQLE LGRFLRSRYE AFLSPEYRRE EVYIRSTDFD RTLESAQANL 130 140 150 160 170 180 AGLFPEAAPG SPEARWRPIP VHTVPVAEDK LLRFPMRSCP RYHELLREAT EAAEYQEALE 190 200 210 220 230 240 GWTGFLSRLE NFTGLSLVGE PLRRAWKVLD TLMCQQAHGL PLPAWASPDV LRTLAQISAL 250 260 270 280 290 300 DIGAHVGPPR AAEKAQLTGG ILLNAILANF SRVQRLGLPL KMVMYSAHDS TLLALQGALG 310 320 330 340 350 360 LYDGHTPPYA ACLGFEFRKH LGNPAKDGGN VTVSLFYRND SAHLPLPLSL PGCPAPCPLG 370 380 390 400 410 420 RFYQLTAPAR PPAHGVSCHG PYEAAIPPAP VVPLLAGAVA VLVALSLGLG LLAWRPGCLR ALGGPV
	Isoform 2 (Variant 3). Checksum: 0C0237096567B30F	FASTA	333	35,403
	10 20 30 40 50 60 MAGLGFWGHP AGPLLLLLLL VLPPRALPEG PLVFVALVFR HGDRAPLASY PMDPHKEVAS 70 80 90 100 110 120 TLWPRGLGQL TTEGVRQQLE LGRFLRSRYE GFLSRLENFT GLSLVGEPLR RAWKVLDTLM 130 140 150 160 170 180 CQQAHGLPLP AWASPDVLRT LAQISALDIG AHVGPPRAAE KAQLTGGILL NAILANFSRV 190 200 210 220 230 240 QRLGLPLKMV MYSAHDSTLL ALQGALGLYD GHTPPYAACL GFEFRKHLGN PAKDGGNVTV 250 260 270 280 290 300 SLFYRNDSAH LPLPLSLPGC PAPCPLGRFY QLTAPARPPA HGVSCHGPYE AAIPPAPVVP 310 320 330 LLAGAVAVLV ALSLGLGLLA WRPGCLRALG GPV
	Isoform 3 (Truncated variant 1) (Truncated variant 2). Checksum: DF4B45C6D2009A31	FASTA	92	9,912
	10 20 30 40 50 60 MAGLGFWGHP AGPLLLLLLL VLPPRALPEG PLVFVALVRR PHPGLPLAPP GLALTSPVPR 70 80 90 YSAMATGPRW PPTPWTHTRR WPPPCGHEAW AS

References

[1]	"Molecular cloning of a novel human acid phosphatase gene (ACPT) that is highly expressed in the testis." Yousef G.M., Diamandis M., Jung K., Diamandis E.P. Genomics 74:385-395(2001) [PubMed: 11414767] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1; 2 AND 3), INDUCTION, TISSUE SPECIFICITY.
[2]	"Regulation of ErbB4 phosphorylation and cleavage by a novel histidine acid phosphatase." Fleisig H., El-Din El-Husseini A., Vincent S.R. Neuroscience 127:91-100(2004) [PubMed: 15219672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, GLYCOSYLATION. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF321918 Genomic DNA. Translation: AAK09393.1. AF321918 Genomic DNA. Translation: AAK09395.1. AF321918 Genomic DNA. Translation: AAK09396.1.
IPI	IPI00001603. IPI00011500. IPI00289055.
RefSeq	NP_149059.1.
UniGene	Hs.293394
3D structure databases
HSSP	HSSP built from PDB template 1ND5 based on UniProtKB P15309.
ModBase	Search...
Proteomic databases
PRIDE	Q9BZG2.
Genome annotation databases
Ensembl	ENSG00000142513. Homo sapiens. [Contig view]
GeneID	93650.
KEGG	hsa:93650.
Organism-specific databases
GeneCards	GC19P055985.
HGNC	HGNC:14376. ACPT.
MIM	606362. gene.
PharmGKB	PA24450.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9BZG2.
OMA	Q9BZG2. EGPLVFV.
Enzyme and pathway databases
BRENDA	3.1.3.2. 247.
Gene expression databases
ArrayExpress	Q9BZG2.
Bgee	Q9BZG2.
CleanEx	HS_ACPT.
GermOnline	ENSG00000142513. Homo sapiens.
Family and domain databases
InterPro	IPR000560. Histidine_acid_Pase. [Graphical view]
Pfam	PF00328. Acid_phosphat_A. 1 hit. [Graphical view]
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	78184.
SOURCE	Search...

Entry information

Entry name

PPAT_HUMAN

Accession

Primary (citable) accession number: Q9BZG2
Secondary accession number(s): Q9BZG3, Q9BZG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)