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Protein

Oxysterol-binding protein-related protein 8

Gene

OSBPL8

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner (PubMed:26206935). Binds oxysterol, 25-hydroxycholesterol and cholesterol (PubMed:17428193, PubMed:17991739, PubMed:21698267).4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei485PhosphatidylserineBy similarity1
Binding sitei540PhosphatidylserineBy similarity1
Binding sitei706Phosphatidylinositol 4-phosphateBy similarity1
Binding sitei710Phosphatidylinositol 4-phosphateBy similarity1
Binding sitei714Phosphatidylinositol 4-phosphateBy similarity1

GO - Molecular functioni

  • cholesterol binding Source: BHF-UCL
  • phosphatidylinositol-4-phosphate binding Source: UniProtKB
  • phosphatidylserine binding Source: UniProtKB
  • phospholipid transporter activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000091039-MONOMER.
ReactomeiR-HSA-1482801. Acyl chain remodelling of PS.

Protein family/group databases

TCDBi2.D.1.1.2. the pi4p/ps counter transporter (p/p-ct) family.

Chemistry databases

SwissLipidsiSLP:000001535.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxysterol-binding protein-related protein 8
Short name:
ORP-8
Short name:
OSBP-related protein 8
Gene namesi
Name:OSBPL8
Synonyms:KIAA14511 Publication, ORP8, OSBP10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:16396. OSBPL8.

Subcellular locationi

Isoform 1 :
Isoform 3 :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei871 – 888HelicalSequence analysisAdd BLAST18

GO - Cellular componenti

  • cortical endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • nuclear membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi514 – 515HH → AA: Impaired lipid countertransport between the endoplasmic reticulum and the plasma membrane. 1 Publication2

Organism-specific databases

DisGeNETi114882.
OpenTargetsiENSG00000091039.
PharmGKBiPA32832.

Polymorphism and mutation databases

BioMutaiOSBPL8.
DMDMi39932732.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001003781 – 889Oxysterol-binding protein-related protein 8Add BLAST889

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei14PhosphoserineCombined sources1
Modified residuei65PhosphoserineCombined sources1
Modified residuei68PhosphoserineCombined sources1
Modified residuei314PhosphoserineCombined sources1
Modified residuei328PhosphoserineCombined sources1
Modified residuei342PhosphoserineCombined sources1
Modified residuei807PhosphoserineCombined sources1
Modified residuei808PhosphoserineCombined sources1
Modified residuei810PhosphoserineCombined sources1
Modified residuei814PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BZF1.
MaxQBiQ9BZF1.
PaxDbiQ9BZF1.
PeptideAtlasiQ9BZF1.
PRIDEiQ9BZF1.

PTM databases

iPTMnetiQ9BZF1.
PhosphoSitePlusiQ9BZF1.
SwissPalmiQ9BZF1.

Miscellaneous databases

PMAP-CutDBQ9BZF1.

Expressioni

Tissue specificityi

Widely expressed (PubMed:11735225). Expressed at higher level in macrophages (PubMed:17991739).2 Publications

Gene expression databases

BgeeiENSG00000091039.
CleanExiHS_OSBPL8.
ExpressionAtlasiQ9BZF1. baseline and differential.
GenevisibleiQ9BZF1. HS.

Organism-specific databases

HPAiHPA001309.

Interactioni

Subunit structurei

Interacts with SPAG5 (PubMed:24424245). Interacts with NUP62 (PubMed:21698267).2 Publications

Protein-protein interaction databases

BioGridi125383. 34 interactors.
DIPiDIP-56130N.
IntActiQ9BZF1. 12 interactors.
MINTiMINT-2816858.
STRINGi9606.ENSP00000261183.

Structurei

Secondary structure

1889
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi151 – 157Combined sources7
Beta strandi160 – 163Combined sources4
Beta strandi165 – 172Combined sources8
Beta strandi175 – 180Combined sources6
Beta strandi182 – 184Combined sources3
Beta strandi189 – 192Combined sources4
Turni203 – 205Combined sources3
Beta strandi209 – 213Combined sources5
Beta strandi244 – 246Combined sources3
Helixi250 – 264Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V88NMR-A149-265[»]
ProteinModelPortaliQ9BZF1.
SMRiQ9BZF1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BZF1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini148 – 265PHPROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni420 – 425Phosphatidylinositol 4-phosphate bindingBy similarity6
Regioni420 – 425Phosphatidylserine bindingBy similarity6
Regioni482 – 485Phosphatidylinositol 4-phosphate bindingBy similarity4
Regioni514 – 515Phosphatidylinositol 4-phosphate bindingBy similarity2

Sequence similaritiesi

Belongs to the OSBP family.Curated
Contains 1 PH domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2210. Eukaryota.
ENOG410XRW6. LUCA.
GeneTreeiENSGT00550000074515.
HOGENOMiHOG000233870.
HOVERGENiHBG053375.
InParanoidiQ9BZF1.
KOiK20464.
OMAiDLYQPSF.
OrthoDBiEOG091G0A3R.
PhylomeDBiQ9BZF1.
TreeFamiTF312807.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000648. Oxysterol-bd.
IPR018494. Oxysterol-bd_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR10972. PTHR10972. 1 hit.
PfamiPF01237. Oxysterol_BP. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS01013. OSBP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BZF1-1) [UniParc]FASTAAdd to basket
Also known as: ORP8L1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGGLADGEP DRTSLLGDSK DVLGPSTVVA NSDESQLLTP GKMSQRQGKE
60 70 80 90 100
AYPTPTKDLH QPSLSPASPH SQGFERGKED ISQNKDESSL SMSKSKSESK
110 120 130 140 150
LYNGSEKDSS TSSKLTKKES LKVQKKNYRE EKKRATKELL STITDPSVIV
160 170 180 190 200
MADWLKIRGT LKSWTKLWCV LKPGVLLIYK TQKNGQWVGT VLLNACEIIE
210 220 230 240 250
RPSKKDGFCF KLFHPLEQSI WAVKGPKGEA VGSITQPLPS SYLIIRATSE
260 270 280 290 300
SDGRCWMDAL ELALKCSSLL KRTMIREGKE HDLSVSSDST HVTFYGLLRA
310 320 330 340 350
NNLHSGDNFQ LNDSEIERQH FKDQDMYSDK SDKENDQEHD ESDNEVMGKS
360 370 380 390 400
EESDTDTSER QDDSYIEPEP VEPLKETTYT EQSHEELGEA GEASQTETVS
410 420 430 440 450
EENKSLIWTL LKQVRPGMDL SKVVLPTFIL EPRSFLDKLS DYYYHADFLS
460 470 480 490 500
EAALEENPYF RLKKVVKWYL SGFYKKPKGL KKPYNPILGE TFRCLWIHPR
510 520 530 540 550
TNSKTFYIAE QVSHHPPISA FYVSNRKDGF CLSGSILAKS KFYGNSLSAI
560 570 580 590 600
LEGEARLTFL NRGEDYVMTM PYAHCKGILY GTMTLELGGT VNITCQKTGY
610 620 630 640 650
SAILEFKLKP FLGSSDCVNQ ISGKLKLGKE VLATLEGHWD SEVFITDKKT
660 670 680 690 700
DNSEVFWNPT PDIKQWRLIR HTVKFEEQGD FESEKLWQRV TRAINAKDQT
710 720 730 740 750
EATQEKYVLE EAQRQAARDR KTKNEEWSCK LFELDPLTGE WHYKFADTRP
760 770 780 790 800
WDPLNDMIQF EKDGVIQTKV KHRTPMVSVP KMKHKPTRQQ KKVAKGYSSP
810 820 830 840 850
EPDIQDSSGS EAQSVKPSTR RKKGIELGDI QSSIESIKQT QEEIKRNIMA
860 870 880
LRNHLVSSTP ATDYFLQQKD YFIIFLLILL QVIINFMFK
Length:889
Mass (Da):101,196
Last modified:December 15, 2003 - v3
Checksum:i79DB8055BD15FE95
GO
Isoform 2 (identifier: Q9BZF1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-29: Missing.

Note: No experimental confirmation available.
Show »
Length:874
Mass (Da):99,714
Checksum:i309510B3F9E184DB
GO
Isoform 3 (identifier: Q9BZF1-3) [UniParc]FASTAAdd to basket
Also known as: ORP8S1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.

Show »
Length:847
Mass (Da):96,956
Checksum:i9DD6AB040C2FD20B
GO

Sequence cautioni

The sequence AAG53411 differs from that shown. Reason: Frameshift at position 30.Curated
The sequence BAA95975 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti347M → V in CAH18345 (PubMed:17974005).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0458011 – 42Missing in isoform 3. CuratedAdd BLAST42
Alternative sequenceiVSP_00912015 – 29Missing in isoform 2. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF392452 mRNA. Translation: AAL40665.1.
AB040884 mRNA. Translation: BAA95975.3. Different initiation.
AK289997 mRNA. Translation: BAF82686.1.
CR749542 mRNA. Translation: CAH18345.1.
AC117491 Genomic DNA. No translation available.
AC122687 Genomic DNA. No translation available.
AC124943 Genomic DNA. No translation available.
BC093834 mRNA. Translation: AAH93834.1.
BC101529 mRNA. Translation: AAI01530.1.
BC111728 mRNA. Translation: AAI11729.1.
AF323730 mRNA. Translation: AAG53411.1. Frameshift.
CCDSiCCDS31862.1. [Q9BZF1-1]
CCDS41814.1. [Q9BZF1-3]
RefSeqiNP_001003712.1. NM_001003712.1. [Q9BZF1-3]
NP_001306581.1. NM_001319652.1. [Q9BZF1-3]
NP_001306584.1. NM_001319655.1.
NP_065892.1. NM_020841.4. [Q9BZF1-1]
XP_005268678.1. XM_005268621.4. [Q9BZF1-1]
XP_006719287.1. XM_006719224.2. [Q9BZF1-3]
UniGeneiHs.430849.

Genome annotation databases

EnsembliENST00000261183; ENSP00000261183; ENSG00000091039. [Q9BZF1-1]
ENST00000393249; ENSP00000376939; ENSG00000091039. [Q9BZF1-3]
ENST00000393250; ENSP00000376940; ENSG00000091039. [Q9BZF1-3]
ENST00000611266; ENSP00000478240; ENSG00000091039. [Q9BZF1-3]
GeneIDi114882.
KEGGihsa:114882.
UCSCiuc001sye.2. human. [Q9BZF1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF392452 mRNA. Translation: AAL40665.1.
AB040884 mRNA. Translation: BAA95975.3. Different initiation.
AK289997 mRNA. Translation: BAF82686.1.
CR749542 mRNA. Translation: CAH18345.1.
AC117491 Genomic DNA. No translation available.
AC122687 Genomic DNA. No translation available.
AC124943 Genomic DNA. No translation available.
BC093834 mRNA. Translation: AAH93834.1.
BC101529 mRNA. Translation: AAI01530.1.
BC111728 mRNA. Translation: AAI11729.1.
AF323730 mRNA. Translation: AAG53411.1. Frameshift.
CCDSiCCDS31862.1. [Q9BZF1-1]
CCDS41814.1. [Q9BZF1-3]
RefSeqiNP_001003712.1. NM_001003712.1. [Q9BZF1-3]
NP_001306581.1. NM_001319652.1. [Q9BZF1-3]
NP_001306584.1. NM_001319655.1.
NP_065892.1. NM_020841.4. [Q9BZF1-1]
XP_005268678.1. XM_005268621.4. [Q9BZF1-1]
XP_006719287.1. XM_006719224.2. [Q9BZF1-3]
UniGeneiHs.430849.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V88NMR-A149-265[»]
ProteinModelPortaliQ9BZF1.
SMRiQ9BZF1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125383. 34 interactors.
DIPiDIP-56130N.
IntActiQ9BZF1. 12 interactors.
MINTiMINT-2816858.
STRINGi9606.ENSP00000261183.

Chemistry databases

SwissLipidsiSLP:000001535.

Protein family/group databases

TCDBi2.D.1.1.2. the pi4p/ps counter transporter (p/p-ct) family.

PTM databases

iPTMnetiQ9BZF1.
PhosphoSitePlusiQ9BZF1.
SwissPalmiQ9BZF1.

Polymorphism and mutation databases

BioMutaiOSBPL8.
DMDMi39932732.

Proteomic databases

EPDiQ9BZF1.
MaxQBiQ9BZF1.
PaxDbiQ9BZF1.
PeptideAtlasiQ9BZF1.
PRIDEiQ9BZF1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261183; ENSP00000261183; ENSG00000091039. [Q9BZF1-1]
ENST00000393249; ENSP00000376939; ENSG00000091039. [Q9BZF1-3]
ENST00000393250; ENSP00000376940; ENSG00000091039. [Q9BZF1-3]
ENST00000611266; ENSP00000478240; ENSG00000091039. [Q9BZF1-3]
GeneIDi114882.
KEGGihsa:114882.
UCSCiuc001sye.2. human. [Q9BZF1-1]

Organism-specific databases

CTDi114882.
DisGeNETi114882.
GeneCardsiOSBPL8.
HGNCiHGNC:16396. OSBPL8.
HPAiHPA001309.
MIMi606736. gene.
neXtProtiNX_Q9BZF1.
OpenTargetsiENSG00000091039.
PharmGKBiPA32832.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2210. Eukaryota.
ENOG410XRW6. LUCA.
GeneTreeiENSGT00550000074515.
HOGENOMiHOG000233870.
HOVERGENiHBG053375.
InParanoidiQ9BZF1.
KOiK20464.
OMAiDLYQPSF.
OrthoDBiEOG091G0A3R.
PhylomeDBiQ9BZF1.
TreeFamiTF312807.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000091039-MONOMER.
ReactomeiR-HSA-1482801. Acyl chain remodelling of PS.

Miscellaneous databases

ChiTaRSiOSBPL8. human.
EvolutionaryTraceiQ9BZF1.
GeneWikiiOSBPL8.
GenomeRNAii114882.
PMAP-CutDBQ9BZF1.
PROiQ9BZF1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000091039.
CleanExiHS_OSBPL8.
ExpressionAtlasiQ9BZF1. baseline and differential.
GenevisibleiQ9BZF1. HS.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000648. Oxysterol-bd.
IPR018494. Oxysterol-bd_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR10972. PTHR10972. 1 hit.
PfamiPF01237. Oxysterol_BP. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS01013. OSBP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOSBL8_HUMAN
AccessioniPrimary (citable) accession number: Q9BZF1
Secondary accession number(s): A8K1T2
, E9PE66, E9PE68, Q52LQ3, Q68D75, Q8WXP8, Q9P277
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: December 15, 2003
Last modified: November 30, 2016
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.