Q9BZE4 (NOG1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleolar GTP-binding protein 1

Alternative name(s):
Chronic renal failure gene protein
GTP-binding protein NGB

Gene names

Name:	GTPBP4
Synonyms:	CRFG, NOG1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	634 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the biogenesis of the 60S ribosomal subunit By similarity.
Subcellular location	Nucleus › nucleolus Ref.2 Ref.7 Ref.8.
Sequence similarities	Belongs to the GTP1/OBG family. NOG subfamily.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of DNA replication Inferred from mutant phenotype PubMed 17210637. Source: HGNC negative regulation of cell migration Inferred from direct assay PubMed 17210637. Source: HGNC negative regulation of cell proliferation Inferred from mutant phenotype PubMed 17210637. Source: HGNC negative regulation of cell-cell adhesion Inferred from direct assay PubMed 17210637. Source: HGNC negative regulation of collagen binding Inferred from mutant phenotype PubMed 17210637. Source: HGNC negative regulation of protein ubiquitination Inferred from direct assay PubMed 17210637. Source: HGNC protein stabilization Inferred from direct assay PubMed 17210637. Source: HGNC regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from direct assay PubMed 17210637. Source: HGNC ribosome biogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from direct assay. Source: HPA nuclear membrane Inferred from direct assay. Source: HPA nucleolus Inferred from direct assay. Source: HPA perinuclear region of cytoplasm Inferred from direct assay PubMed 17210637. Source: HGNC
Molecular_function	GTP binding Inferred from direct assay PubMed 17210637. Source: HGNC GTPase activity Inferred from direct assay PubMed 17210637. Source: HGNC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NF2	P35240	9	EBI-1056249,EBI-1014472

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.8
	Chain	2 – 634	633	Nucleolar GTP-binding protein 1		PRO_0000195023
Regions
	Nucleotide binding	175 – 182	8	GTP Potential
	Nucleotide binding	221 – 225	5	GTP Potential
	Nucleotide binding	289 – 292	4	GTP Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.8
	Modified residue	103	1	N6-acetyllysine Ref.13
	Modified residue	468	1	Phosphoserine Ref.11 Ref.12 Ref.14 Ref.16
	Modified residue	470	1	Phosphoserine Ref.11 Ref.12 Ref.14 Ref.16
	Modified residue	472	1	Phosphoserine Ref.11 Ref.12 Ref.14
	Modified residue	522	1	N6-acetyllysine Ref.13
	Modified residue	558	1	Phosphoserine Ref.11
Natural variations
	Natural variant	525	1	R → H. Ref.3 Corresponds to variant rs3207775 [ dbSNP | Ensembl ].		VAR_068801
Experimental info
	Sequence conflict	593	1	Missing in AAD09830. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9BZE4 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 0A05E65E65D6DB62	FASTA	634	73,964
	10 20 30 40 50 60 MAHYNFKKIT VVPSAKDFID LTLSKTQRKT PTVIHKHYQI HRIRHFYMRK VKFTQQNYHD 70 80 90 100 110 120 RLSQILTDFP KLDDIHPFYA DLMNILYDKD HYKLALGQIN IAKNLVDNVA KDYVRLMKYG 130 140 150 160 170 180 DSLYRCKQLK RAALGRMCTV IKRQKQSLEY LEQVRQHLSR LPTIDPNTRT LLLCGYPNVG 190 200 210 220 230 240 KSSFINKVTR ADVDVQPYAF TTKSLFVGHM DYKYLRWQVV DTPGILDHPL EDRNTIEMQA 250 260 270 280 290 300 ITALAHLRAA VLYVMDLSEQ CGHGLREQLE LFQNIRPLFI NKPLIVVANK CDVKRIAELS 310 320 330 340 350 360 EDDQKIFTDL QSEGFPVIET STLTEEGVIK VKTEACDRLL AHRVETKMKG NKVNEVLNRL 370 380 390 400 410 420 HLAIPTRRDD KERPPFIPEG VVARRKRMET EESRKKRERD LELEMGDDYI LDLQKYWDLM 430 440 450 460 470 480 NLSEKHDKIP EIWEGHNIAD YIDPAIMKKL EELEKEEELR TAAGEYDSVS ESEDEEMLEI 490 500 510 520 530 540 RQLAKQIREK KKLKILESKE KNTQGPRMPR TAKKVQRTVL EKEMRSLGVD MDDKDDAHYA 550 560 570 580 590 600 VQARRSRSIT RKRKREDSAP PSSVARSGSC SRTPRDVSGL RDVKMVKKAK TMMKNAQKKM 610 620 630 NRLGKKGEAD RHVFDMKPKH LLSGKRKAGK KDRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a novel GTP-binding protein, NGB." Cheng J.Q., Cao C.H., Testa J.R., Golemis E.A., Jiang C., Yuan W., Nicosia S.V. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification of a novel nuclear guanosine triphosphate-binding protein differentially expressed in renal disease." Laping N.J., Olson B.A., Zhu Y. J. Am. Soc. Nephrol. 12:883-890(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION. Tissue: Kidney.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT HIS-525.
[4]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[7]	"Functional proteomic analysis of human nucleolus." Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J. Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	Bienvenut W.V. Submitted (OCT-2004) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-7; 9-25; 37-42; 53-71; 94-111; 119-125; 146-155; 161-169; 191-213; 217-248; 267-276; 296-330; 353-359; 372-384; 398-425; 429-448; 450-460 AND 526-544, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, SUBCELLULAR LOCATION, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[10]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-468; SER-470; SER-472 AND SER-558, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-468; SER-470 AND SER-472, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[13]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-103 AND LYS-522, MASS SPECTROMETRY.
[14]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-468; SER-470 AND SER-472, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-468 AND SER-470, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF120334 mRNA. Translation: AAD09830.1. AF325353 mRNA. Translation: AAK13444.1. AK001548 mRNA. Translation: BAG50937.1. AK001552 mRNA. Translation: BAA91752.1. AL359878, AC022536 Genomic DNA. Translation: CAI13664.1. CH471072 Genomic DNA. Translation: EAW86526.1. BC038975 mRNA. Translation: AAH38975.1.
IPI	IPI00385042.
RefSeq	NP_036473.2. NM_012341.2.
UniGene	Hs.215766.
3D structure databases
ProteinModelPortal	Q9BZE4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BZE4. 10 interactions.
STRING	9606.ENSP00000354040.
PTM databases
PhosphoSite	Q9BZE4.
Polymorphism databases
DMDM	17368711.
2D gel databases
SWISS-2DPAGE	Q9BZE4.
Proteomic databases
PaxDb	Q9BZE4.
PRIDE	Q9BZE4.
Protocols and materials databases
DNASU	23560.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000360803; ENSP00000354040; ENSG00000107937.
GeneID	23560.
KEGG	hsa:23560.
UCSC	uc001ift.3. human.
Organism-specific databases
CTD	23560.
GeneCards	GC10P001034.
HGNC	HGNC:21535. GTPBP4.
HPA	HPA039618.
neXtProt	NX_Q9BZE4.
PharmGKB	PA134922009.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1084.
HOGENOM	HOG000164071.
HOVERGEN	HBG052642.
KO	K06943.
OMA	HDEWKED.
OrthoDB	EOG48WC1N.
Gene expression databases
ArrayExpress	Q9BZE4.
Bgee	Q9BZE4.
CleanEx	HS_GTPBP4.
Genevestigator	Q9BZE4.
GermOnline	ENSG00000107937. Homo sapiens.
Family and domain databases
InterPro	IPR006073. GTP_binding_domain. IPR024926. NOG1. IPR010674. NOG1_Rossman_fold_dom. IPR012973. NOG_C. IPR005225. Small_GTP-bd_dom. [Graphical view]
Pfam	PF06858. NOG1. 1 hit. PF08155. NOGCT. 1 hit. [Graphical view]
PIRSF	PIRSF038919. NOG1. 1 hit.
PRINTS	PR00326. GTP1OBG.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	GTPBP4. human.
GenomeRNAi	23560.
NextBio	35468847.

Entry information

Entry name

NOG1_HUMAN

Accession

Primary (citable) accession number: Q9BZE4
Secondary accession number(s): B3KMC5 , O95446, Q5T3R8, Q9NVJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2001
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 118 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.