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Q9BZE2

- PUS3_HUMAN

UniProt

Q9BZE2 - PUS3_HUMAN

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Protein

tRNA pseudouridine(38/39) synthase

Gene

PUS3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs.By similarity

Catalytic activityi

tRNA uridine(38/39) = tRNA pseudouridine(38/39).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei118 – 1181NucleophileBy similarity
Binding sitei195 – 1951SubstrateBy similarity

GO - Molecular functioni

  1. pseudouridine synthase activity Source: Ensembl
  2. RNA binding Source: InterPro

GO - Biological processi

  1. tRNA pseudouridine synthesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine(38/39) synthase (EC:5.4.99.45)
Alternative name(s):
tRNA pseudouridine synthase 3
tRNA pseudouridylate synthase 3
tRNA-uridine isomerase 3
Gene namesi
Name:PUS3
ORF Names:FKSG32
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:25461. PUS3.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134874048.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 481480tRNA pseudouridine(38/39) synthasePRO_0000057520Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei456 – 4561Phosphothreonine1 Publication
Modified residuei468 – 4681Phosphothreonine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9BZE2.
PaxDbiQ9BZE2.
PRIDEiQ9BZE2.

PTM databases

PhosphoSiteiQ9BZE2.

Expressioni

Gene expression databases

BgeeiQ9BZE2.
CleanExiHS_PUS3.
ExpressionAtlasiQ9BZE2. baseline and differential.
GenevestigatoriQ9BZE2.

Organism-specific databases

HPAiHPA038040.

Interactioni

Protein-protein interaction databases

BioGridi123665. 2 interactions.
STRINGi9606.ENSP00000227474.

Structurei

3D structure databases

ProteinModelPortaliQ9BZE2.
SMRiQ9BZE2. Positions 62-374.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0101.
GeneTreeiENSGT00510000047444.
HOGENOMiHOG000199032.
HOVERGENiHBG053769.
InParanoidiQ9BZE2.
KOiK01855.
OMAiQKPQYSM.
OrthoDBiEOG7S4X6C.
PhylomeDBiQ9BZE2.
TreeFamiTF314428.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BZE2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAYNDTDRNQ TEKLLKRVRE LEQEVQRLKK EQAKNKEDSN IRENSAGAGK
60 70 80 90 100
TKRAFDFSAH GRRHVALRIA YMGWGYQGFA SQENTNNTIE EKLFEALTKT
110 120 130 140 150
RLVESRQTSN YHRCGRTDKG VSAFGQVISL DLRSQFPRGR DSEDFNVKEE
160 170 180 190 200
ANAAAEEIRY THILNRVLPP DIRILAWAPV EPSFSARFSC LERTYRYFFP
210 220 230 240 250
RADLDIVTMD YAAQKYVGTH DFRNLCKMDV ANGVINFQRT ILSAQVQLVG
260 270 280 290 300
QSPGEGRWQE PFQLCQFEVT GQAFLYHQVR CMMAILFLIG QGMEKPEIID
310 320 330 340 350
ELLNIEKNPQ KPQYSMAVEF PLVLYDCKFE NVKWIYDQEA QEFNITHLQQ
360 370 380 390 400
LWANHAVKTH MLYSMLQGLD TVPVPCGIGP KMDGMTEWGN VKPSVIKQTS
410 420 430 440 450
AFVEGVKMRT YKPLMDRPKC QGLESRIQHF VRRGRIEHPH LFHEEETKAK
460 470 480
RDCNDTLEEE NTNLETPTKR VCVDTEIKSI I
Length:481
Mass (Da):55,647
Last modified:February 20, 2007 - v3
Checksum:i54B93653DB333535
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti145 – 1451F → L in BAB70990. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti3 – 31Y → D.2 Publications
Corresponds to variant rs622756 [ dbSNP | Ensembl ].
VAR_030836
Natural varianti46 – 461A → S.2 Publications
Corresponds to variant rs549990 [ dbSNP | Ensembl ].
VAR_030837
Natural varianti460 – 4601E → D.1 Publication
Corresponds to variant rs3088241 [ dbSNP | Ensembl ].
VAR_030838

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF325689 mRNA. Translation: AAG50280.1.
AK055702 mRNA. Translation: BAB70990.1.
AK314251 mRNA. Translation: BAG36917.1.
BC004822 mRNA. Translation: AAH04822.1.
BC013427 mRNA. Translation: AAH13427.2.
CCDSiCCDS8466.1.
RefSeqiNP_001258914.1. NM_001271985.1.
NP_112597.3. NM_031307.3.
UniGeneiHs.660922.

Genome annotation databases

EnsembliENST00000227474; ENSP00000227474; ENSG00000110060.
ENST00000530811; ENSP00000432386; ENSG00000110060.
GeneIDi83480.
KEGGihsa:83480.
UCSCiuc001qcy.2. human.

Polymorphism databases

DMDMi126302589.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF325689 mRNA. Translation: AAG50280.1 .
AK055702 mRNA. Translation: BAB70990.1 .
AK314251 mRNA. Translation: BAG36917.1 .
BC004822 mRNA. Translation: AAH04822.1 .
BC013427 mRNA. Translation: AAH13427.2 .
CCDSi CCDS8466.1.
RefSeqi NP_001258914.1. NM_001271985.1.
NP_112597.3. NM_031307.3.
UniGenei Hs.660922.

3D structure databases

ProteinModelPortali Q9BZE2.
SMRi Q9BZE2. Positions 62-374.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 123665. 2 interactions.
STRINGi 9606.ENSP00000227474.

PTM databases

PhosphoSitei Q9BZE2.

Polymorphism databases

DMDMi 126302589.

Proteomic databases

MaxQBi Q9BZE2.
PaxDbi Q9BZE2.
PRIDEi Q9BZE2.

Protocols and materials databases

DNASUi 83480.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000227474 ; ENSP00000227474 ; ENSG00000110060 .
ENST00000530811 ; ENSP00000432386 ; ENSG00000110060 .
GeneIDi 83480.
KEGGi hsa:83480.
UCSCi uc001qcy.2. human.

Organism-specific databases

CTDi 83480.
GeneCardsi GC11M125763.
H-InvDB HIX0010246.
HGNCi HGNC:25461. PUS3.
HPAi HPA038040.
neXtProti NX_Q9BZE2.
PharmGKBi PA134874048.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0101.
GeneTreei ENSGT00510000047444.
HOGENOMi HOG000199032.
HOVERGENi HBG053769.
InParanoidi Q9BZE2.
KOi K01855.
OMAi QKPQYSM.
OrthoDBi EOG7S4X6C.
PhylomeDBi Q9BZE2.
TreeFami TF314428.

Miscellaneous databases

GenomeRNAii 83480.
NextBioi 72425.
PROi Q9BZE2.

Gene expression databases

Bgeei Q9BZE2.
CleanExi HS_PUS3.
ExpressionAtlasi Q9BZE2. baseline and differential.
Genevestigatori Q9BZE2.

Family and domain databases

Gene3Di 3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPi MF_00171. TruA.
InterProi IPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view ]
PANTHERi PTHR11142. PTHR11142. 1 hit.
Pfami PF01416. PseudoU_synth_1. 2 hits.
[Graphical view ]
SUPFAMi SSF55120. SSF55120. 1 hit.
TIGRFAMsi TIGR00071. hisT_truA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of FKSG32, a novel gene expressed in lung carcinoid."
    Wang Y.-G., Gong L.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASP-3 AND SER-46.
    Tissue: Lung.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASP-3; SER-46 AND ASP-460.
    Tissue: Pancreas and Placenta.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-456 AND THR-468, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPUS3_HUMAN
AccessioniPrimary (citable) accession number: Q9BZE2
Secondary accession number(s): B2RAM0
, Q96D17, Q96J23, Q96NB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: February 20, 2007
Last modified: October 29, 2014
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3