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Q9BZE2

- PUS3_HUMAN

UniProt

Q9BZE2 - PUS3_HUMAN

Protein

tRNA pseudouridine(38/39) synthase

Gene

PUS3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 3 (20 Feb 2007)
      Previous versions | rss
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    Functioni

    Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs.By similarity

    Catalytic activityi

    tRNA uridine(38/39) = tRNA pseudouridine(38/39).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei118 – 1181NucleophileBy similarity
    Binding sitei195 – 1951SubstrateBy similarity

    GO - Molecular functioni

    1. pseudouridine synthase activity Source: Ensembl
    2. RNA binding Source: InterPro

    GO - Biological processi

    1. tRNA pseudouridine synthesis Source: Ensembl

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA pseudouridine(38/39) synthase (EC:5.4.99.45)
    Alternative name(s):
    tRNA pseudouridine synthase 3
    tRNA pseudouridylate synthase 3
    tRNA-uridine isomerase 3
    Gene namesi
    Name:PUS3
    ORF Names:FKSG32
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:25461. PUS3.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134874048.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 481480tRNA pseudouridine(38/39) synthasePRO_0000057520Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei456 – 4561Phosphothreonine1 Publication
    Modified residuei468 – 4681Phosphothreonine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9BZE2.
    PaxDbiQ9BZE2.
    PRIDEiQ9BZE2.

    PTM databases

    PhosphoSiteiQ9BZE2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9BZE2.
    BgeeiQ9BZE2.
    CleanExiHS_PUS3.
    GenevestigatoriQ9BZE2.

    Organism-specific databases

    HPAiHPA038040.

    Interactioni

    Protein-protein interaction databases

    BioGridi123665. 2 interactions.
    STRINGi9606.ENSP00000227474.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BZE2.
    SMRiQ9BZE2. Positions 62-374.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0101.
    HOGENOMiHOG000199032.
    HOVERGENiHBG053769.
    InParanoidiQ9BZE2.
    KOiK01855.
    OMAiQKPQYSM.
    OrthoDBiEOG7S4X6C.
    PhylomeDBiQ9BZE2.
    TreeFamiTF314428.

    Family and domain databases

    Gene3Di3.30.70.580. 1 hit.
    3.30.70.660. 1 hit.
    HAMAPiMF_00171. TruA.
    InterProiIPR020103. PsdUridine_synth_cat_dom.
    IPR001406. PsdUridine_synth_TruA.
    IPR020097. PsdUridine_synth_TruA_a/b_dom.
    IPR020095. PsdUridine_synth_TruA_C.
    IPR020094. PsdUridine_synth_TruA_N.
    [Graphical view]
    PANTHERiPTHR11142. PTHR11142. 1 hit.
    PfamiPF01416. PseudoU_synth_1. 2 hits.
    [Graphical view]
    SUPFAMiSSF55120. SSF55120. 1 hit.
    TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9BZE2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAYNDTDRNQ TEKLLKRVRE LEQEVQRLKK EQAKNKEDSN IRENSAGAGK    50
    TKRAFDFSAH GRRHVALRIA YMGWGYQGFA SQENTNNTIE EKLFEALTKT 100
    RLVESRQTSN YHRCGRTDKG VSAFGQVISL DLRSQFPRGR DSEDFNVKEE 150
    ANAAAEEIRY THILNRVLPP DIRILAWAPV EPSFSARFSC LERTYRYFFP 200
    RADLDIVTMD YAAQKYVGTH DFRNLCKMDV ANGVINFQRT ILSAQVQLVG 250
    QSPGEGRWQE PFQLCQFEVT GQAFLYHQVR CMMAILFLIG QGMEKPEIID 300
    ELLNIEKNPQ KPQYSMAVEF PLVLYDCKFE NVKWIYDQEA QEFNITHLQQ 350
    LWANHAVKTH MLYSMLQGLD TVPVPCGIGP KMDGMTEWGN VKPSVIKQTS 400
    AFVEGVKMRT YKPLMDRPKC QGLESRIQHF VRRGRIEHPH LFHEEETKAK 450
    RDCNDTLEEE NTNLETPTKR VCVDTEIKSI I 481
    Length:481
    Mass (Da):55,647
    Last modified:February 20, 2007 - v3
    Checksum:i54B93653DB333535
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti145 – 1451F → L in BAB70990. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti3 – 31Y → D.2 Publications
    Corresponds to variant rs622756 [ dbSNP | Ensembl ].
    VAR_030836
    Natural varianti46 – 461A → S.2 Publications
    Corresponds to variant rs549990 [ dbSNP | Ensembl ].
    VAR_030837
    Natural varianti460 – 4601E → D.1 Publication
    Corresponds to variant rs3088241 [ dbSNP | Ensembl ].
    VAR_030838

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF325689 mRNA. Translation: AAG50280.1.
    AK055702 mRNA. Translation: BAB70990.1.
    AK314251 mRNA. Translation: BAG36917.1.
    BC004822 mRNA. Translation: AAH04822.1.
    BC013427 mRNA. Translation: AAH13427.2.
    CCDSiCCDS8466.1.
    RefSeqiNP_001258914.1. NM_001271985.1.
    NP_112597.3. NM_031307.3.
    UniGeneiHs.660922.

    Genome annotation databases

    EnsembliENST00000227474; ENSP00000227474; ENSG00000110060.
    ENST00000530811; ENSP00000432386; ENSG00000110060.
    GeneIDi83480.
    KEGGihsa:83480.
    UCSCiuc001qcy.2. human.

    Polymorphism databases

    DMDMi126302589.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF325689 mRNA. Translation: AAG50280.1 .
    AK055702 mRNA. Translation: BAB70990.1 .
    AK314251 mRNA. Translation: BAG36917.1 .
    BC004822 mRNA. Translation: AAH04822.1 .
    BC013427 mRNA. Translation: AAH13427.2 .
    CCDSi CCDS8466.1.
    RefSeqi NP_001258914.1. NM_001271985.1.
    NP_112597.3. NM_031307.3.
    UniGenei Hs.660922.

    3D structure databases

    ProteinModelPortali Q9BZE2.
    SMRi Q9BZE2. Positions 62-374.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 123665. 2 interactions.
    STRINGi 9606.ENSP00000227474.

    PTM databases

    PhosphoSitei Q9BZE2.

    Polymorphism databases

    DMDMi 126302589.

    Proteomic databases

    MaxQBi Q9BZE2.
    PaxDbi Q9BZE2.
    PRIDEi Q9BZE2.

    Protocols and materials databases

    DNASUi 83480.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000227474 ; ENSP00000227474 ; ENSG00000110060 .
    ENST00000530811 ; ENSP00000432386 ; ENSG00000110060 .
    GeneIDi 83480.
    KEGGi hsa:83480.
    UCSCi uc001qcy.2. human.

    Organism-specific databases

    CTDi 83480.
    GeneCardsi GC11M125763.
    H-InvDB HIX0010246.
    HGNCi HGNC:25461. PUS3.
    HPAi HPA038040.
    neXtProti NX_Q9BZE2.
    PharmGKBi PA134874048.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0101.
    HOGENOMi HOG000199032.
    HOVERGENi HBG053769.
    InParanoidi Q9BZE2.
    KOi K01855.
    OMAi QKPQYSM.
    OrthoDBi EOG7S4X6C.
    PhylomeDBi Q9BZE2.
    TreeFami TF314428.

    Miscellaneous databases

    GenomeRNAii 83480.
    NextBioi 72425.
    PROi Q9BZE2.

    Gene expression databases

    ArrayExpressi Q9BZE2.
    Bgeei Q9BZE2.
    CleanExi HS_PUS3.
    Genevestigatori Q9BZE2.

    Family and domain databases

    Gene3Di 3.30.70.580. 1 hit.
    3.30.70.660. 1 hit.
    HAMAPi MF_00171. TruA.
    InterProi IPR020103. PsdUridine_synth_cat_dom.
    IPR001406. PsdUridine_synth_TruA.
    IPR020097. PsdUridine_synth_TruA_a/b_dom.
    IPR020095. PsdUridine_synth_TruA_C.
    IPR020094. PsdUridine_synth_TruA_N.
    [Graphical view ]
    PANTHERi PTHR11142. PTHR11142. 1 hit.
    Pfami PF01416. PseudoU_synth_1. 2 hits.
    [Graphical view ]
    SUPFAMi SSF55120. SSF55120. 1 hit.
    TIGRFAMsi TIGR00071. hisT_truA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of FKSG32, a novel gene expressed in lung carcinoid."
      Wang Y.-G., Gong L.
      Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASP-3 AND SER-46.
      Tissue: Lung.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASP-3; SER-46 AND ASP-460.
      Tissue: Pancreas and Placenta.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-456 AND THR-468, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPUS3_HUMAN
    AccessioniPrimary (citable) accession number: Q9BZE2
    Secondary accession number(s): B2RAM0
    , Q96D17, Q96J23, Q96NB4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 27, 2004
    Last sequence update: February 20, 2007
    Last modified: October 1, 2014
    This is version 98 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3