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Protein

tRNA pseudouridine(38/39) synthase

Gene

PUS3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs.By similarity

Catalytic activityi

tRNA uridine(38/39) = tRNA pseudouridine(38/39).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei118NucleophileBy similarity1
Binding sitei195SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciZFISH:HS12710-MONOMER.
ReactomeiR-HSA-6782315. tRNA modification in the nucleus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine(38/39) synthase (EC:5.4.99.45)
Alternative name(s):
tRNA pseudouridine synthase 3
tRNA pseudouridylate synthase 3
tRNA-uridine isomerase 3
Gene namesi
Name:PUS3
ORF Names:FKSG32
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:25461. PUS3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000110060.
PharmGKBiPA134874048.

Polymorphism and mutation databases

BioMutaiPUS3.
DMDMi126302589.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000575202 – 481tRNA pseudouridine(38/39) synthaseAdd BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei456PhosphothreonineCombined sources1
Modified residuei466PhosphothreonineCombined sources1
Modified residuei468PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BZE2.
MaxQBiQ9BZE2.
PaxDbiQ9BZE2.
PeptideAtlasiQ9BZE2.
PRIDEiQ9BZE2.

PTM databases

iPTMnetiQ9BZE2.
PhosphoSitePlusiQ9BZE2.

Expressioni

Gene expression databases

BgeeiENSG00000110060.
CleanExiHS_PUS3.
ExpressionAtlasiQ9BZE2. baseline and differential.
GenevisibleiQ9BZE2. HS.

Organism-specific databases

HPAiHPA038040.
HPA063802.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000227474.

Structurei

3D structure databases

ProteinModelPortaliQ9BZE2.
SMRiQ9BZE2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2554. Eukaryota.
COG0101. LUCA.
GeneTreeiENSGT00510000047444.
HOGENOMiHOG000199032.
HOVERGENiHBG053769.
InParanoidiQ9BZE2.
KOiK01855.
OMAiKRVCVDT.
OrthoDBiEOG091G05MF.
PhylomeDBiQ9BZE2.
TreeFamiTF314428.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9BZE2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYNDTDRNQ TEKLLKRVRE LEQEVQRLKK EQAKNKEDSN IRENSAGAGK
60 70 80 90 100
TKRAFDFSAH GRRHVALRIA YMGWGYQGFA SQENTNNTIE EKLFEALTKT
110 120 130 140 150
RLVESRQTSN YHRCGRTDKG VSAFGQVISL DLRSQFPRGR DSEDFNVKEE
160 170 180 190 200
ANAAAEEIRY THILNRVLPP DIRILAWAPV EPSFSARFSC LERTYRYFFP
210 220 230 240 250
RADLDIVTMD YAAQKYVGTH DFRNLCKMDV ANGVINFQRT ILSAQVQLVG
260 270 280 290 300
QSPGEGRWQE PFQLCQFEVT GQAFLYHQVR CMMAILFLIG QGMEKPEIID
310 320 330 340 350
ELLNIEKNPQ KPQYSMAVEF PLVLYDCKFE NVKWIYDQEA QEFNITHLQQ
360 370 380 390 400
LWANHAVKTH MLYSMLQGLD TVPVPCGIGP KMDGMTEWGN VKPSVIKQTS
410 420 430 440 450
AFVEGVKMRT YKPLMDRPKC QGLESRIQHF VRRGRIEHPH LFHEEETKAK
460 470 480
RDCNDTLEEE NTNLETPTKR VCVDTEIKSI I
Length:481
Mass (Da):55,647
Last modified:February 20, 2007 - v3
Checksum:i54B93653DB333535
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145F → L in BAB70990 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0308363Y → D.2 PublicationsCorresponds to variant rs622756dbSNPEnsembl.1
Natural variantiVAR_03083746A → S.2 PublicationsCorresponds to variant rs549990dbSNPEnsembl.1
Natural variantiVAR_030838460E → D.1 PublicationCorresponds to variant rs3088241dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF325689 mRNA. Translation: AAG50280.1.
AK055702 mRNA. Translation: BAB70990.1.
AK314251 mRNA. Translation: BAG36917.1.
BC004822 mRNA. Translation: AAH04822.1.
BC013427 mRNA. Translation: AAH13427.2.
CCDSiCCDS8466.1.
RefSeqiNP_001258914.1. NM_001271985.1.
NP_112597.3. NM_031307.3.
UniGeneiHs.660922.

Genome annotation databases

EnsembliENST00000227474; ENSP00000227474; ENSG00000110060.
ENST00000530811; ENSP00000432386; ENSG00000110060.
GeneIDi83480.
KEGGihsa:83480.
UCSCiuc001qcy.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF325689 mRNA. Translation: AAG50280.1.
AK055702 mRNA. Translation: BAB70990.1.
AK314251 mRNA. Translation: BAG36917.1.
BC004822 mRNA. Translation: AAH04822.1.
BC013427 mRNA. Translation: AAH13427.2.
CCDSiCCDS8466.1.
RefSeqiNP_001258914.1. NM_001271985.1.
NP_112597.3. NM_031307.3.
UniGeneiHs.660922.

3D structure databases

ProteinModelPortaliQ9BZE2.
SMRiQ9BZE2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000227474.

PTM databases

iPTMnetiQ9BZE2.
PhosphoSitePlusiQ9BZE2.

Polymorphism and mutation databases

BioMutaiPUS3.
DMDMi126302589.

Proteomic databases

EPDiQ9BZE2.
MaxQBiQ9BZE2.
PaxDbiQ9BZE2.
PeptideAtlasiQ9BZE2.
PRIDEiQ9BZE2.

Protocols and materials databases

DNASUi83480.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000227474; ENSP00000227474; ENSG00000110060.
ENST00000530811; ENSP00000432386; ENSG00000110060.
GeneIDi83480.
KEGGihsa:83480.
UCSCiuc001qcy.3. human.

Organism-specific databases

CTDi83480.
GeneCardsiPUS3.
H-InvDBHIX0010246.
HGNCiHGNC:25461. PUS3.
HPAiHPA038040.
HPA063802.
MIMi616283. gene.
neXtProtiNX_Q9BZE2.
OpenTargetsiENSG00000110060.
PharmGKBiPA134874048.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2554. Eukaryota.
COG0101. LUCA.
GeneTreeiENSGT00510000047444.
HOGENOMiHOG000199032.
HOVERGENiHBG053769.
InParanoidiQ9BZE2.
KOiK01855.
OMAiKRVCVDT.
OrthoDBiEOG091G05MF.
PhylomeDBiQ9BZE2.
TreeFamiTF314428.

Enzyme and pathway databases

BioCyciZFISH:HS12710-MONOMER.
ReactomeiR-HSA-6782315. tRNA modification in the nucleus and cytosol.

Miscellaneous databases

GenomeRNAii83480.
PROiQ9BZE2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000110060.
CleanExiHS_PUS3.
ExpressionAtlasiQ9BZE2. baseline and differential.
GenevisibleiQ9BZE2. HS.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPUS3_HUMAN
AccessioniPrimary (citable) accession number: Q9BZE2
Secondary accession number(s): B2RAM0
, Q96D17, Q96J23, Q96NB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: February 20, 2007
Last modified: November 30, 2016
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.