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Q9BZE2 (PUS3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA pseudouridine(38/39) synthase

EC=5.4.99.45
Alternative name(s):
tRNA pseudouridine synthase 3
tRNA pseudouridylate synthase 3
tRNA-uridine isomerase 3
Gene names
Name:PUS3
ORF Names:FKSG32
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length481 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171

Catalytic activity

tRNA uridine(38/39) = tRNA pseudouridine(38/39). HAMAP-Rule MF_00171

Subcellular location

Nucleus Potential HAMAP-Rule MF_00171.

Sequence similarities

Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   Coding sequence diversityPolymorphism
   Molecular functionIsomerase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 481480tRNA pseudouridine(38/39) synthase HAMAP-Rule MF_00171
PRO_0000057520

Sites

Active site1181Nucleophile By similarity
Binding site1951Substrate By similarity

Amino acid modifications

Modified residue21N-acetylalanine Ref.5
Modified residue4561Phosphothreonine Ref.4
Modified residue4681Phosphothreonine Ref.4

Natural variations

Natural variant31Y → D. Ref.2 Ref.3
Corresponds to variant rs622756 [ dbSNP | Ensembl ].
VAR_030836
Natural variant461A → S. Ref.2 Ref.3
Corresponds to variant rs549990 [ dbSNP | Ensembl ].
VAR_030837
Natural variant4601E → D. Ref.3
Corresponds to variant rs3088241 [ dbSNP | Ensembl ].
VAR_030838

Experimental info

Sequence conflict1451F → L in BAB70990. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9BZE2 [UniParc].

Last modified February 20, 2007. Version 3.
Checksum: 54B93653DB333535

FASTA48155,647
        10         20         30         40         50         60 
MAYNDTDRNQ TEKLLKRVRE LEQEVQRLKK EQAKNKEDSN IRENSAGAGK TKRAFDFSAH 

        70         80         90        100        110        120 
GRRHVALRIA YMGWGYQGFA SQENTNNTIE EKLFEALTKT RLVESRQTSN YHRCGRTDKG 

       130        140        150        160        170        180 
VSAFGQVISL DLRSQFPRGR DSEDFNVKEE ANAAAEEIRY THILNRVLPP DIRILAWAPV 

       190        200        210        220        230        240 
EPSFSARFSC LERTYRYFFP RADLDIVTMD YAAQKYVGTH DFRNLCKMDV ANGVINFQRT 

       250        260        270        280        290        300 
ILSAQVQLVG QSPGEGRWQE PFQLCQFEVT GQAFLYHQVR CMMAILFLIG QGMEKPEIID 

       310        320        330        340        350        360 
ELLNIEKNPQ KPQYSMAVEF PLVLYDCKFE NVKWIYDQEA QEFNITHLQQ LWANHAVKTH 

       370        380        390        400        410        420 
MLYSMLQGLD TVPVPCGIGP KMDGMTEWGN VKPSVIKQTS AFVEGVKMRT YKPLMDRPKC 

       430        440        450        460        470        480 
QGLESRIQHF VRRGRIEHPH LFHEEETKAK RDCNDTLEEE NTNLETPTKR VCVDTEIKSI 


I 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of FKSG32, a novel gene expressed in lung carcinoid."
Wang Y.-G., Gong L.
Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASP-3 AND SER-46.
Tissue: Lung.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ASP-3; SER-46 AND ASP-460.
Tissue: Pancreas and Placenta.
[4]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-456 AND THR-468, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF325689 mRNA. Translation: AAG50280.1.
AK055702 mRNA. Translation: BAB70990.1.
AK314251 mRNA. Translation: BAG36917.1.
BC004822 mRNA. Translation: AAH04822.1.
BC013427 mRNA. Translation: AAH13427.2.
RefSeqNP_001258914.1. NM_001271985.1.
NP_112597.3. NM_031307.3.
UniGeneHs.660922.

3D structure databases

ProteinModelPortalQ9BZE2.
SMRQ9BZE2. Positions 62-374.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123665. 1 interaction.
STRING9606.ENSP00000227474.

PTM databases

PhosphoSiteQ9BZE2.

Polymorphism databases

DMDM126302589.

Proteomic databases

PaxDbQ9BZE2.
PRIDEQ9BZE2.

Protocols and materials databases

DNASU83480.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000227474; ENSP00000227474; ENSG00000110060.
ENST00000530811; ENSP00000432386; ENSG00000110060.
GeneID83480.
KEGGhsa:83480.
UCSCuc001qcy.2. human.

Organism-specific databases

CTD83480.
GeneCardsGC11M125763.
H-InvDBHIX0010246.
HGNCHGNC:25461. PUS3.
HPAHPA038040.
neXtProtNX_Q9BZE2.
PharmGKBPA134874048.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0101.
HOGENOMHOG000199032.
HOVERGENHBG053769.
InParanoidQ9BZE2.
KOK01855.
OMAQKPQYSM.
OrthoDBEOG7S4X6C.
PhylomeDBQ9BZE2.
TreeFamTF314428.

Gene expression databases

ArrayExpressQ9BZE2.
BgeeQ9BZE2.
CleanExHS_PUS3.
GenevestigatorQ9BZE2.

Family and domain databases

Gene3D3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPMF_00171. TruA.
InterProIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERPTHR11142. PTHR11142. 1 hit.
PfamPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
SUPFAMSSF55120. SSF55120. 1 hit.
TIGRFAMsTIGR00071. hisT_truA. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi83480.
NextBio72425.
PROQ9BZE2.

Entry information

Entry namePUS3_HUMAN
AccessionPrimary (citable) accession number: Q9BZE2
Secondary accession number(s): B2RAM0 expand/collapse secondary AC list , Q96D17, Q96J23, Q96NB4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: February 20, 2007
Last modified: March 19, 2014
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

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List of human entries with polymorphisms or disease mutations

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Human chromosome 11: entries, gene names and cross-references to MIM