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Protein

Kinetochore protein Nuf2

Gene

NUF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:12438418, PubMed:14654001, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:17535814). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).8 Publications

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: UniProtKB
  • mitotic nuclear division Source: UniProtKB-KW
  • sister chromatid cohesion Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein Nuf2
Short name:
hNuf2
Short name:
hNuf2R
Short name:
hsNuf2
Alternative name(s):
Cell division cycle-associated protein 1
Gene namesi
Name:NUF2
Synonyms:CDCA1, NUF2R
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:14621. NUF2.

Subcellular locationi

  • Nucleus
  • Chromosomecentromerekinetochore

  • Note: Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. NDC80 is required for efficient kinetochore localization.

GO - Cellular componenti

  • chromosome, centromeric region Source: UniProtKB
  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • cytosol Source: Reactome
  • membrane Source: UniProtKB
  • Ndc80 complex Source: UniProtKB
  • nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi83540.
OpenTargetsiENSG00000143228.
PharmGKBiPA162398215.

Polymorphism and mutation databases

BioMutaiNUF2.
DMDMi115311829.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002498131 – 464Kinetochore protein Nuf2Add BLAST464

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei171PhosphoserineCombined sources1
Modified residuei247PhosphoserineCombined sources1

Post-translational modificationi

Can be phosphorylated by AURKA and AURKB.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BZD4.
MaxQBiQ9BZD4.
PaxDbiQ9BZD4.
PeptideAtlasiQ9BZD4.
PRIDEiQ9BZD4.

PTM databases

iPTMnetiQ9BZD4.
PhosphoSitePlusiQ9BZD4.

Expressioni

Gene expression databases

BgeeiENSG00000143228.
CleanExiHS_NUF2.
ExpressionAtlasiQ9BZD4. baseline and differential.
GenevisibleiQ9BZD4. HS.

Organism-specific databases

HPAiHPA059692.

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. May interact with AURKB/Aurora-B. Directly interacts with CENPE; this interaction determines CENPE kinetochore localization.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CENPEQ022249EBI-724102,EBI-1375040
NDC80O1477713EBI-724102,EBI-715849

Protein-protein interaction databases

BioGridi123673. 48 interactors.
DIPiDIP-36119N.
IntActiQ9BZD4. 42 interactors.
MINTiMINT-1390492.
STRINGi9606.ENSP00000271452.

Structurei

Secondary structure

1464
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 21Combined sources11
Helixi25 – 29Combined sources5
Helixi33 – 35Combined sources3
Beta strandi37 – 39Combined sources3
Helixi42 – 57Combined sources16
Helixi62 – 64Combined sources3
Helixi76 – 79Combined sources4
Turni80 – 82Combined sources3
Helixi83 – 98Combined sources16
Helixi106 – 110Combined sources5
Helixi114 – 144Combined sources31
Helixi147 – 164Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VE7X-ray2.88C/D1-169[»]
3IZ0electron microscopy-D/F1-169[»]
ProteinModelPortaliQ9BZD4.
SMRiQ9BZD4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BZD4.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 385Interaction with the N-terminus of NDC80Add BLAST385
Regioni386 – 464Interaction with the C-terminus of NDC80 and the SPBC24-SPBC25 subcomplexAdd BLAST79

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili147 – 345Sequence analysisAdd BLAST199
Coiled coili389 – 459Sequence analysisAdd BLAST71

Sequence similaritiesi

Belongs to the NUF2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4438. Eukaryota.
ENOG410ZI4C. LUCA.
GeneTreeiENSGT00390000004199.
HOGENOMiHOG000290701.
HOVERGENiHBG082069.
InParanoidiQ9BZD4.
KOiK11548.
OMAiDCYAKID.
OrthoDBiEOG091G0YDU.
PhylomeDBiQ9BZD4.
TreeFamiTF101067.

Family and domain databases

InterProiIPR005549. Kinetochore_Nuf2.
[Graphical view]
PfamiPF03800. Nuf2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BZD4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METLSFPRYN VAEIVIHIRN KILTGADGKN LTKNDLYPNP KPEVLHMIYM
60 70 80 90 100
RALQIVYGIR LEHFYMMPVN SEVMYPHLME GFLPFSNLVT HLDSFLPICR
110 120 130 140 150
VNDFETADIL CPKAKRTSRF LSGIINFIHF REACRETYME FLWQYKSSAD
160 170 180 190 200
KMQQLNAAHQ EALMKLERLD SVPVEEQEEF KQLSDGIQEL QQSLNQDFHQ
210 220 230 240 250
KTIVLQEGNS QKKSNISEKT KRLNELKLSV VSLKEIQESL KTKIVDSPEK
260 270 280 290 300
LKNYKEKMKD TVQKLKNARQ EVVEKYEIYG DSVDCLPSCQ LEVQLYQKKI
310 320 330 340 350
QDLSDNREKL ASILKESLNL EDQIESDESE LKKLKTEENS FKRLMIVKKE
360 370 380 390 400
KLATAQFKIN KKHEDVKQYK RTVIEDCNKV QEKRGAVYER VTTINQEIQK
410 420 430 440 450
IKLGIQQLKD AAEREKLKSQ EIFLNLKTAL EKYHDGIEKA AEDSYAKIDE
460
KTAELKRKMF KMST
Length:464
Mass (Da):54,304
Last modified:September 19, 2006 - v2
Checksum:i250BFED5AD87153B
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027490229S → L.2 PublicationsCorresponds to variant rs11802875dbSNPEnsembl.1
Natural variantiVAR_027491239S → R.Corresponds to variant rs16852767dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050577 mRNA. Translation: BAB59141.1.
AB050578 mRNA. Translation: BAB59142.1.
AF326731 mRNA. Translation: AAK01426.1.
AK093348 mRNA. Translation: BAC04140.1.
AL592435 Genomic DNA. Translation: CAI16706.1.
BC008489 mRNA. Translation: AAH08489.1.
BC021171 mRNA. Translation: AAH21171.2.
CCDSiCCDS1245.1.
RefSeqiNP_113611.2. NM_031423.3.
NP_663735.2. NM_145697.2.
UniGeneiHs.651950.

Genome annotation databases

EnsembliENST00000271452; ENSP00000271452; ENSG00000143228.
ENST00000367900; ENSP00000356875; ENSG00000143228.
GeneIDi83540.
KEGGihsa:83540.
UCSCiuc001gcq.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050577 mRNA. Translation: BAB59141.1.
AB050578 mRNA. Translation: BAB59142.1.
AF326731 mRNA. Translation: AAK01426.1.
AK093348 mRNA. Translation: BAC04140.1.
AL592435 Genomic DNA. Translation: CAI16706.1.
BC008489 mRNA. Translation: AAH08489.1.
BC021171 mRNA. Translation: AAH21171.2.
CCDSiCCDS1245.1.
RefSeqiNP_113611.2. NM_031423.3.
NP_663735.2. NM_145697.2.
UniGeneiHs.651950.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VE7X-ray2.88C/D1-169[»]
3IZ0electron microscopy-D/F1-169[»]
ProteinModelPortaliQ9BZD4.
SMRiQ9BZD4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123673. 48 interactors.
DIPiDIP-36119N.
IntActiQ9BZD4. 42 interactors.
MINTiMINT-1390492.
STRINGi9606.ENSP00000271452.

PTM databases

iPTMnetiQ9BZD4.
PhosphoSitePlusiQ9BZD4.

Polymorphism and mutation databases

BioMutaiNUF2.
DMDMi115311829.

Proteomic databases

EPDiQ9BZD4.
MaxQBiQ9BZD4.
PaxDbiQ9BZD4.
PeptideAtlasiQ9BZD4.
PRIDEiQ9BZD4.

Protocols and materials databases

DNASUi83540.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000271452; ENSP00000271452; ENSG00000143228.
ENST00000367900; ENSP00000356875; ENSG00000143228.
GeneIDi83540.
KEGGihsa:83540.
UCSCiuc001gcq.2. human.

Organism-specific databases

CTDi83540.
DisGeNETi83540.
GeneCardsiNUF2.
HGNCiHGNC:14621. NUF2.
HPAiHPA059692.
MIMi611772. gene.
neXtProtiNX_Q9BZD4.
OpenTargetsiENSG00000143228.
PharmGKBiPA162398215.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4438. Eukaryota.
ENOG410ZI4C. LUCA.
GeneTreeiENSGT00390000004199.
HOGENOMiHOG000290701.
HOVERGENiHBG082069.
InParanoidiQ9BZD4.
KOiK11548.
OMAiDCYAKID.
OrthoDBiEOG091G0YDU.
PhylomeDBiQ9BZD4.
TreeFamiTF101067.

Enzyme and pathway databases

ReactomeiR-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2500257. Resolution of Sister Chromatid Cohesion.
R-HSA-5663220. RHO GTPases Activate Formins.
R-HSA-68877. Mitotic Prometaphase.

Miscellaneous databases

EvolutionaryTraceiQ9BZD4.
GeneWikiiNUF2.
GenomeRNAii83540.
PROiQ9BZD4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000143228.
CleanExiHS_NUF2.
ExpressionAtlasiQ9BZD4. baseline and differential.
GenevisibleiQ9BZD4. HS.

Family and domain databases

InterProiIPR005549. Kinetochore_Nuf2.
[Graphical view]
PfamiPF03800. Nuf2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUF2_HUMAN
AccessioniPrimary (citable) accession number: Q9BZD4
Secondary accession number(s): Q8WU69, Q96HJ4, Q96Q78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: November 30, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.