Q9BZD4 (NUF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinetochore protein Nuf2
Short name=hNuf2
Short name=hNuf2R
Short name=hsNuf2

Alternative name(s):
Cell division cycle-associated protein 1

Gene names

Name:	NUF2
Synonyms:	CDCA1, NUF2R

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.14
Subunit structure	Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. May interact with AURKB/Aurora-B.
Subcellular location	Nucleus. Chromosome › centromere › kinetochore. Note: Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. Ref.7 Ref.8 Ref.10 Ref.13
Post-translational modification	Can be phosphorylated by AURKA and AURKB. Ref.12 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19
Sequence similarities	Belongs to the NUF2 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Centromere Chromosome Kinetochore Nucleus
Coding sequence diversity	Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Non-traceable author statement Ref.2. Source: UniProtKB mitotic prometaphase Traceable author statement. Source: Reactome
Cellular component	Ndc80 complex Inferred from direct assay Ref.11. Source: UniProtKB condensed chromosome kinetochore Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Traceable author statement. Source: Reactome nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CENPE	Q02224	9	EBI-724102,EBI-1375040
NDC80	O14777	10	EBI-724102,EBI-715849

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 464

464

Kinetochore protein Nuf2

PRO_0000249813

Regions

Region

1 – 385

385

Interaction with the N-terminus of NDC80

Region

386 – 464

79

Interaction with the C-terminus of NDC80 and the SPBC24-SPBC25 subcomplex

Coiled coil

147 – 345

199

Potential

Coiled coil

389 – 459

71

Potential

Amino acid modifications

Modified residue

247

1

Phosphoserine Ref.15 Ref.16 Ref.18 Ref.19

Modified residue

261

1

Phosphothreonine Ref.17

Natural variations

Natural variant

229

1

S → L. Ref.1 Ref.2
Corresponds to variant rs11802875 [ dbSNP | Ensembl ].

VAR_027490

Natural variant

239

1

S → R.
Corresponds to variant rs16852767 [ dbSNP | Ensembl ].

VAR_027491

Secondary structure

1

.

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464

Helix Strand Turn

Details...

Helix

11 – 21

11

Helix

33 – 35

3

Beta strand

37 – 39

3

Helix

42 – 56

15

Helix

62 – 64

3

Helix

76 – 79

4

Helix

83 – 98

16

Helix

106 – 110

5

Helix

114 – 121

8

Helix

125 – 144

20

Helix

147 – 164

18

Sequences

	Sequence		Length	Mass (Da)
	Q9BZD4 [UniParc]. Last modified September 19, 2006. Version 2. Checksum: 250BFED5AD87153B	FASTA	464	54,304
	10 20 30 40 50 60 METLSFPRYN VAEIVIHIRN KILTGADGKN LTKNDLYPNP KPEVLHMIYM RALQIVYGIR 70 80 90 100 110 120 LEHFYMMPVN SEVMYPHLME GFLPFSNLVT HLDSFLPICR VNDFETADIL CPKAKRTSRF 130 140 150 160 170 180 LSGIINFIHF REACRETYME FLWQYKSSAD KMQQLNAAHQ EALMKLERLD SVPVEEQEEF 190 200 210 220 230 240 KQLSDGIQEL QQSLNQDFHQ KTIVLQEGNS QKKSNISEKT KRLNELKLSV VSLKEIQESL 250 260 270 280 290 300 KTKIVDSPEK LKNYKEKMKD TVQKLKNARQ EVVEKYEIYG DSVDCLPSCQ LEVQLYQKKI 310 320 330 340 350 360 QDLSDNREKL ASILKESLNL EDQIESDESE LKKLKTEENS FKRLMIVKKE KLATAQFKIN 370 380 390 400 410 420 KKHEDVKQYK RTVIEDCNKV QEKRGAVYER VTTINQEIQK IKLGIQQLKD AAEREKLKSQ 430 440 450 460 EIFLNLKTAL EKYHDGIEKA AEDSYAKIDE KTAELKRKMF KMST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint." Nabetani A., Koujin T., Tsutsumi C., Haraguchi T., Hiraoka Y. Chromosoma 110:322-334(2001) [PubMed: 11685532] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LEU-229.
[2]	"The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation." Wigge P.A., Kilmartin J.V. J. Cell Biol. 152:349-360(2001) [PubMed: 11266451] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LEU-229.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Chronic myeloid leukemia cell and Lung.
[6]	"hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells." DeLuca J.G., Moree B., Hickey J.M., Kilmartin J.V., Salmon E.D. J. Cell Biol. 159:549-555(2002) [PubMed: 12438418] [Abstract] Cited for: FUNCTION.
[7]	"Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores." DeLuca J.G., Howell B.J., Canman J.C., Hickey J.M., Fang G., Salmon E.D. Curr. Biol. 13:2103-2109(2003) [PubMed: 14654001] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1." Stucke V.M., Baumann C., Nigg E.A. Chromosoma 113:1-15(2004) [PubMed: 15235793] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]	"The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo." Joseph J., Liu S.-T., Jablonski S.A., Yen T.J., Dasso M. Curr. Biol. 14:611-617(2004) [PubMed: 15062103] [Abstract] Cited for: FUNCTION.
[10]	"Timing and checkpoints in the regulation of mitotic progression." Meraldi P., Draviam V.M., Sorger P.K. Dev. Cell 7:45-60(2004) [PubMed: 15239953] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[11]	"Identification of two novel components of the human NDC80 kinetochore complex." Bharadwaj R., Qi W., Yu H. J. Biol. Chem. 279:13076-13085(2004) [PubMed: 14699129] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE NDC80 COMPLEX.
[12]	"Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model." Tien A.-C., Lin M.-H., Su L.-J., Hong Y.-R., Cheng T.-S., Lee Y.-C.G., Lin W.-J., Still I.H., Huang C.-Y.F. Mol. Cell. Proteomics 3:93-104(2004) [PubMed: 14602875] [Abstract] Cited for: INTERACTION WITH AURKB AND NDC80, PHOSPHORYLATION BY AURKA AND AURKB.
[13]	"Architecture of the human Ndc80-Hec1 complex, a critical constituent of the outer kinetochore." Ciferri C., De Luca J., Monzani S., Ferrari K.J., Ristic D., Wyman C., Stark H., Kilmartin J., Salmon E.D., Musacchio A. J. Biol. Chem. 280:29088-29095(2005) [PubMed: 15961401] [Abstract] Cited for: CHARACTERIZATION OF THE NDC80 COMPLEX, SUBCELLULAR LOCATION.
[14]	"Hec1 and Nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites." DeLuca J.G., Dong Y., Hergert P., Strauss J., Hickey J.M., Salmon E.D., McEwen B.F. Mol. Biol. Cell 16:519-531(2005) [PubMed: 15548592] [Abstract] Cited for: FUNCTION.
[15]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[16]	"Phosphoproteome analysis of the human mitotic spindle." Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R. Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, MASS SPECTROMETRY. Tissue: Cervix adenocarcinoma.
[17]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-261, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[18]	"Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis." Wang B., Malik R., Nigg E.A., Korner R. Anal. Chem. 80:9526-9533(2008) [PubMed: 19007248] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[19]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[20]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB050577 mRNA. Translation: BAB59141.1.
AB050578 mRNA. Translation: BAB59142.1.
AF326731 mRNA. Translation: AAK01426.1.
AK093348 mRNA. Translation: BAC04140.1.
AL592435 Genomic DNA. Translation: CAI16706.1.
BC008489 mRNA. Translation: AAH08489.1.
BC021171 mRNA. Translation: AAH21171.2.

IPI

IPI00305887.

RefSeq

NP_113611.2. NM_031423.3.
NP_663735.2. NM_145697.2.

UniGene

Hs.651950.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VE7	X-ray	2.88	C/D	1-169	[»]
3IZ0	electron microscopy	-	D/F	1-169	[»]

ProteinModelPortal

Q9BZD4.

SMR

Q9BZD4. Positions 4-194.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9BZD4. 16 interactions.

MINT

MINT-1390492.

STRING

Q9BZD4.

PTM databases

PhosphoSite

Q9BZD4.

Polymorphism databases

DMDM

115311829.

Proteomic databases

PeptideAtlas

Q9BZD4.

PRIDE

Q9BZD4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000271452; ENSP00000271452; ENSG00000143228.
ENST00000367900; ENSP00000356875; ENSG00000143228.

GeneID

83540.

KEGG

hsa:83540.

UCSC

uc001gcq.1. human.

Organism-specific databases

CTD

83540.

GeneCards

GC01P163291.

H-InvDB

HIX0001271.

HGNC

HGNC:14621. NUF2.

neXtProt

NX_Q9BZD4.

PharmGKB

PA162398215.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG10841.

GeneTree

ENSGT00390000004199.

HOGENOM

HBG713178.

HOVERGEN

HBG082069.

InParanoid

Q9BZD4.

OMA

DILYPKA.

OrthoDB

EOG4D7Z5K.

PhylomeDB

Q9BZD4.

Enzyme and pathway databases

Reactome

REACT_152. Cell Cycle, Mitotic.
REACT_383. DNA Replication.

Gene expression databases

ArrayExpress

Q9BZD4.

Bgee

Q9BZD4.

CleanEx

HS_NUF2.

Genevestigator

Q9BZD4.

GermOnline

ENSG00000143228. Homo sapiens.

Family and domain databases

InterPro

IPR005549. Kinetochore_Nuf2.
[Graphical view]

KO

K11548.

Pfam

PF03800. Nuf2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

72453.

Entry information

Entry name

NUF2_HUMAN

Accession

Primary (citable) accession number: Q9BZD4
Secondary accession number(s): Q8WU69, Q96HJ4, Q96Q78

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	September 19, 2006
Last modified:	January 25, 2012
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.