Q9BYV6 (TRI55_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tripartite motif-containing protein 55

Alternative name(s):
Muscle-specific RING finger protein 2
Short name=MuRF-2
Short name=MuRF2
RING finger protein 29

Gene names

Name:	TRIM55
Synonyms:	MURF2, RNF29

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May regulate gene expression and protein turnover in muscle cells By similarity.
Subunit structure	Homooligomer and heterooligomer Probable. Interacts with titin/TTN. Interacts with myosins. Interacts with SQSTM1 and NBR1. Isoform 4 may not able to interact with isoform 1, isoform 2 and isoform 3. Probably interacts with TRIM63 and TRIM54. Ref.1 Ref.2 Ref.7
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Nuclear under atrophic conditions and upon mechanical signals. Localizes to the sarcomeric M-band in cardiomyocytes. Colocalizes in part with microtubules By similarity.
Tissue specificity	Highly expressed in muscle. Low-level expression in liver. Ref.1
Sequence similarities	Contains 1 B box-type zinc finger. Contains 1 COS domain. Contains 1 RING-type zinc finger.
Caution	It is uncertain whether Met-1 or Met-17 is the initiator.
Sequence caution	The sequence AAP35876.1 differs from that shown. Reason: Erroneous initiation. The sequence CAC32839.1 differs from that shown. Reason: Erroneous initiation. The sequence CAC32840.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Muscle protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Non-traceable author statement Ref.1. Source: UniProtKB nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	signal transducer activity Non-traceable author statement Ref.1. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-2341179,EBI-2341179

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BYV6-1)

Also known as: p60;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BYV6-2)

Also known as: p50;

The sequence of this isoform differs from the canonical sequence as follows:
     412-507: Missing.

Isoform 3 (identifier: Q9BYV6-3)

Also known as: p60B;

The sequence of this isoform differs from the canonical sequence as follows:
     509-548: IGFEAPPLQGQAAAPASGSGADSEPARHIFSFSWLNSLNE → ELVICLALLAFLILHYIWSQIQCLIFTLMDWI

Isoform 4 (identifier: Q9BYV6-4)

Also known as: p27;

The sequence of this isoform differs from the canonical sequence as follows:
     202-508: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	Tripartite motif-containing protein 55		PRO_0000056286
Regions
	Domain	269 – 327	59	COS
	Zinc finger	10 – 66	57	RING-type
	Zinc finger	103 – 145	43	B box-type
	Coiled coil	168 – 248	81	Potential
Natural variations
	Alternative sequence	202 – 508	307	Missing in isoform 4.		VSP_015996
	Alternative sequence	412 – 507	96	Missing in isoform 2.		VSP_015997
	Alternative sequence	509 – 548	40	IGFEA…NSLNE → ELVICLALLAFLILHYIWSQ IQCLIFTLMDWI in isoform 3.		VSP_015998
	Natural variant	343	1	K → R. Corresponds to variant rs7843605 [ dbSNP | Ensembl ].		VAR_052144
Experimental info
	Sequence conflict	102	1	E → G in CAC43019. Ref.2
	Sequence conflict	102	1	E → G in CAC43020. Ref.2
	Sequence conflict	345	1	G → E in CAC43019. Ref.2
	Sequence conflict	345	1	G → E in CAC43020. Ref.2
	Sequence conflict	345	1	G → E in CAD24432. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (p60) [UniParc]. Last modified October 25, 2005. Version 2. Checksum: 2AA2E4D4F5C3E3A1	FASTA	548	60,466
	10 20 30 40 50 60 MSASLNYKSF SKEQQTMDNL EKQLICPICL EMFTKPVVIL PCQHNLCRKC ASDIFQASNP 70 80 90 100 110 120 YLPTRGGTTM ASGGRFRCPS CRHEVVLDRH GVYGLQRNLL VENIIDIYKQ ESTRPEKKSD 130 140 150 160 170 180 QPMCEEHEEE RINIYCLNCE VPTCSLCKVF GAHKDCQVAP LTHVFQRQKS ELSDGIAILV 190 200 210 220 230 240 GSNDRVQGVI SQLEDTCKTI EECCRKQKQE LCEKFDYLYG ILEERKNEMT QVITRTQEEK 250 260 270 280 290 300 LEHVRALIKK YSDHLENVSK LVESGIQFMD EPEMAVFLQN AKTLLKKISE ASKAFQMEKI 310 320 330 340 350 360 EHGYENMNHF TVNLNREEKI IREIDFYRED EDEEEEEGGE GEKEGEGEVG GEAVEVEEVE 370 380 390 400 410 420 NVQTEFPGED ENPEKASELS QVELQAAPGA LPVSSPEPPP ALPPAADAPV TQGEVVPTGS 430 440 450 460 470 480 EQTTESETPV PAAAETADPL FYPSWYKGQT RKATTNPPCT PGSEGLGQIG PPGSEDSNVR 490 500 510 520 530 540 KAEVAAAAAS ERAAVSGKET SAPAATSQIG FEAPPLQGQA AAPASGSGAD SEPARHIFSF SWLNSLNE
	Isoform 2 (p50) [UniParc]. Checksum: 47E656DC77628A45	FASTA	452	50,823
	10 20 30 40 50 60 MSASLNYKSF SKEQQTMDNL EKQLICPICL EMFTKPVVIL PCQHNLCRKC ASDIFQASNP 70 80 90 100 110 120 YLPTRGGTTM ASGGRFRCPS CRHEVVLDRH GVYGLQRNLL VENIIDIYKQ ESTRPEKKSD 130 140 150 160 170 180 QPMCEEHEEE RINIYCLNCE VPTCSLCKVF GAHKDCQVAP LTHVFQRQKS ELSDGIAILV 190 200 210 220 230 240 GSNDRVQGVI SQLEDTCKTI EECCRKQKQE LCEKFDYLYG ILEERKNEMT QVITRTQEEK 250 260 270 280 290 300 LEHVRALIKK YSDHLENVSK LVESGIQFMD EPEMAVFLQN AKTLLKKISE ASKAFQMEKI 310 320 330 340 350 360 EHGYENMNHF TVNLNREEKI IREIDFYRED EDEEEEEGGE GEKEGEGEVG GEAVEVEEVE 370 380 390 400 410 420 NVQTEFPGED ENPEKASELS QVELQAAPGA LPVSSPEPPP ALPPAADAPV TQIGFEAPPL 430 440 450 QGQAAAPASG SGADSEPARH IFSFSWLNSL NE
	Isoform 3 (p60B) [UniParc]. Checksum: 5F017CFB82982E81	FASTA	540	60,174
	10 20 30 40 50 60 MSASLNYKSF SKEQQTMDNL EKQLICPICL EMFTKPVVIL PCQHNLCRKC ASDIFQASNP 70 80 90 100 110 120 YLPTRGGTTM ASGGRFRCPS CRHEVVLDRH GVYGLQRNLL VENIIDIYKQ ESTRPEKKSD 130 140 150 160 170 180 QPMCEEHEEE RINIYCLNCE VPTCSLCKVF GAHKDCQVAP LTHVFQRQKS ELSDGIAILV 190 200 210 220 230 240 GSNDRVQGVI SQLEDTCKTI EECCRKQKQE LCEKFDYLYG ILEERKNEMT QVITRTQEEK 250 260 270 280 290 300 LEHVRALIKK YSDHLENVSK LVESGIQFMD EPEMAVFLQN AKTLLKKISE ASKAFQMEKI 310 320 330 340 350 360 EHGYENMNHF TVNLNREEKI IREIDFYRED EDEEEEEGGE GEKEGEGEVG GEAVEVEEVE 370 380 390 400 410 420 NVQTEFPGED ENPEKASELS QVELQAAPGA LPVSSPEPPP ALPPAADAPV TQGEVVPTGS 430 440 450 460 470 480 EQTTESETPV PAAAETADPL FYPSWYKGQT RKATTNPPCT PGSEGLGQIG PPGSEDSNVR 490 500 510 520 530 540 KAEVAAAAAS ERAAVSGKET SAPAATSQEL VICLALLAFL ILHYIWSQIQ CLIFTLMDWI
	Isoform 4 (p27) [UniParc]. Checksum: 21951B79C1B89B37	FASTA	241	26,841
	10 20 30 40 50 60 MSASLNYKSF SKEQQTMDNL EKQLICPICL EMFTKPVVIL PCQHNLCRKC ASDIFQASNP 70 80 90 100 110 120 YLPTRGGTTM ASGGRFRCPS CRHEVVLDRH GVYGLQRNLL VENIIDIYKQ ESTRPEKKSD 130 140 150 160 170 180 QPMCEEHEEE RINIYCLNCE VPTCSLCKVF GAHKDCQVAP LTHVFQRQKS ELSDGIAILV 190 200 210 220 230 240 GSNDRVQGVI SQLEDTCKTI EIGFEAPPLQ GQAAAPASGS GADSEPARHI FSFSWLNSLN E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain." Centner T., Yano J., Kimura E., McElhinny A.S., Pelin K., Witt C.C., Bang M.-L., Trombitas K., Granzier H., Gregorio C.C., Sorimachi H., Labeit S. J. Mol. Biol. 306:717-726(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, INTERACTION WITH TRIM63 AND TRIM54. Tissue: Heart.
[2]	"Transient association of titin and myosin with microtubules in nascent myofibrils directed by the MURF2 RING-finger protein." Pizon V., Iakovenko A., van der Ven P.F.M., Kelly R., Fatu C., Fuerst D.O., Karsenti E., Gautel M. J. Cell Sci. 115:4469-4482(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), OLIGOMERIZATION, INTERACTION WITH TTN AND MYOSIN. Tissue: Heart muscle and Skeletal muscle.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Heart.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Muscle.
[6]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 17-548 (ISOFORM 2).
[7]	"The kinase domain of titin controls muscle gene expression and protein turnover." Lange S., Xiang F., Yakovenko A., Vihola A., Hackman P., Rostkova E., Kristensen J., Brandmeier B., Franzen G., Hedberg B., Gunnarsson L.G., Hughes S.M., Marchand S., Sejersen T., Richard I., Edstroem L., Ehler E., Udd B., Gautel M. Science 308:1599-1603(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TTN; SQSTM1 AND NBR1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ291712 mRNA. Translation: CAC32839.1. Different initiation. AJ291712 mRNA. Translation: CAC32840.1. Different initiation. AJ243488 mRNA. Translation: CAC43019.1. AJ243489 mRNA. Translation: CAC43020.1. AJ277493 mRNA. Translation: CAC81835.1. AJ431704 mRNA. Translation: CAD24432.1. AK091310 mRNA. Translation: BAG52332.1. AK091728 mRNA. Translation: BAG52405.1. CH471068 Genomic DNA. Translation: EAW86894.1. CH471068 Genomic DNA. Translation: EAW86895.1. BC007750 mRNA. Translation: AAH07750.2. BT007212 mRNA. Translation: AAP35876.1. Different initiation.
IPI	IPI00011473. IPI00216729. IPI00375443. IPI00375444.
RefSeq	NP_149047.2. NM_033058.2. NP_908973.1. NM_184085.1. NP_908974.1. NM_184086.1. NP_908975.1. NM_184087.1.
UniGene	Hs.85524.
3D structure databases
ProteinModelPortal	Q9BYV6.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BYV6. 11 interactions.
MINT	MINT-4723378.
PTM databases
PhosphoSite	Q9BYV6.
Polymorphism databases
DMDM	78099806.
Proteomic databases
PaxDb	Q9BYV6.
PRIDE	Q9BYV6.
Protocols and materials databases
DNASU	84675.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000276573; ENSP00000276573; ENSG00000147573. ENST00000315962; ENSP00000323913; ENSG00000147573. ENST00000350034; ENSP00000332302; ENSG00000147573. ENST00000353317; ENSP00000297348; ENSG00000147573.
GeneID	84675.
KEGG	hsa:84675.
UCSC	uc003xvu.3. human. uc003xvv.3. human. uc003xvw.3. human. uc003xvx.3. human.
Organism-specific databases
CTD	84675.
GeneCards	GC08P067039.
HGNC	HGNC:14215. TRIM55.
HPA	HPA038793.
MIM	606469. gene.
neXtProt	NX_Q9BYV6.
PharmGKB	PA34432.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG310224.
HOVERGEN	HBG071242.
InParanoid	Q9BYV6.
KO	K10654.
OMA	EIDFYRE.
OrthoDB	EOG4WQ12R.
PhylomeDB	Q9BYV6.
Gene expression databases
Bgee	Q9BYV6.
CleanEx	HS_TRIM55.
Genevestigator	Q9BYV6.
GermOnline	ENSG00000147573. Homo sapiens.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR017903. COS_domain. IPR000315. Znf_B-box. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF00643. zf-B_box. 1 hit. [Graphical view]
SMART	SM00336. BBOX. 1 hit. SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS51262. COS. 1 hit. PS50119. ZF_BBOX. 1 hit. PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	84675.
NextBio	74689.
SOURCE	Search...

Entry information

Entry name

TRI55_HUMAN

Accession

Primary (citable) accession number: Q9BYV6
Secondary accession number(s): B3KRC0 , B3KRJ3, Q53XX3, Q8IUD9, Q8IUE4, Q96DV2, Q96DV3, Q9BYV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	October 25, 2005
Last modified:	May 1, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.