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Q9BYT8 (NEUL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neurolysin, mitochondrial

EC=3.4.24.16
Alternative name(s):
Angiotensin-binding protein
Microsomal endopeptidase
Short name=MEP
Mitochondrial oligopeptidase M
Neurotensin endopeptidase
Gene names
Name:NLN
Synonyms:AGTBP, KIAA1226
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length704 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A By similarity.

Catalytic activity

Preferential cleavage in neurotensin: 10-Pro-|-Tyr-11.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Mitochondrion intermembrane space By similarity. Cytoplasm By similarity.

Sequence similarities

Belongs to the peptidase M3 family.

Ontologies

Keywords
   Cellular componentCytoplasm
Mitochondrion
   Coding sequence diversityPolymorphism
   DomainTransit peptide
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmitochondrial intermembrane space

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

peptide binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3737Mitochondrion By similarity
Chain38 – 704667Neurolysin, mitochondrial
PRO_0000028575

Sites

Active site4981 By similarity
Metal binding4971Zinc; catalytic By similarity
Metal binding5011Zinc; catalytic By similarity
Metal binding5041Zinc; catalytic By similarity

Amino acid modifications

Modified residue6641N6-acetyllysine Ref.5

Natural variations

Natural variant791G → S.
Corresponds to variant rs34339013 [ dbSNP | Ensembl ].
VAR_062224
Natural variant3231S → G.
Corresponds to variant rs34063558 [ dbSNP | Ensembl ].
VAR_054002
Natural variant3721K → R.
Corresponds to variant rs6863012 [ dbSNP | Ensembl ].
VAR_054003
Natural variant4171S → G.
Corresponds to variant rs2289884 [ dbSNP | Ensembl ].
VAR_054004
Natural variant7041P → S.
Corresponds to variant rs6860508 [ dbSNP | Ensembl ].
VAR_024594

Sequences

Sequence LengthMass (Da)Tools
Q9BYT8 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 80136688D79BBEDF

FASTA70480,652
        10         20         30         40         50         60 
MIARCLLAVR SLRRVGGSRI LLRMTLGREV MSPLQAMSSY TVAGRNVLRW DLSPEQIKTR 

        70         80         90        100        110        120 
TEELIVQTKQ VYDAVGMLGI EEVTYENCLQ ALADVEVKYI VERTMLDFPQ HVSSDKEVRA 

       130        140        150        160        170        180 
ASTEADKRLS RFDIEMSMRG DIFERIVHLQ ETCDLGKIKP EARRYLEKSI KMGKRNGLHL 

       190        200        210        220        230        240 
PEQVQNEIKS MKKRMSELCI DFNKNLNEDD TFLVFSKAEL GALPDDFIDS LEKTDDDKYK 

       250        260        270        280        290        300 
ITLKYPHYFP VMKKCCIPET RRRMEMAFNT RCKEENTIIL QQLLPLRTKV AKLLGYSTHA 

       310        320        330        340        350        360 
DFVLEMNTAK STSRVTAFLD DLSQKLKPLG EAEREFILNL KKKECKDRGF EYDGKINAWD 

       370        380        390        400        410        420 
LYYYMTQTEE LKYSIDQEFL KEYFPIEVVT EGLLNTYQEL LGLSFEQMTD AHVWNKSVTL 

       430        440        450        460        470        480 
YTVKDKATGE VLGQFYLDLY PREGKYNHAA CFGLQPGCLL PDGSRMMAVA ALVVNFSQPV 

       490        500        510        520        530        540 
AGRPSLLRHD EVRTYFHEFG HVMHQICAQT DFARFSGTNV ETDFVEVPSQ MLENWVWDVD 

       550        560        570        580        590        600 
SLRRLSKHYK DGSPIADDLL EKLVASRLVN TGLLTLRQIV LSKVDQSLHT NTSLDAASEY 

       610        620        630        640        650        660 
AKYCSEILGV AATPGTNMPA TFGHLAGGYD GQYYGYLWSE VFSMDMFYSC FKKEGIMNPE 

       670        680        690        700 
VGMKYRNLIL KPGGSLDGMD MLHNFLKREP NQKAFLMSRG LHAP 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of human neurolysin, an oligopeptidase of family M3."
Chen J.M., Rawlings N.D., Barrett A.J.
Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O.
DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[4]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ300837 mRNA. Translation: CAC27329.1.
AB033052 mRNA. Translation: BAA86540.2.
RefSeqNP_065777.1. NM_020726.4.
UniGeneHs.247460.

3D structure databases

ProteinModelPortalQ9BYT8.
SMRQ9BYT8. Positions 37-701.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121555. 3 interactions.
IntActQ9BYT8. 1 interaction.
STRING9606.ENSP00000370372.

Protein family/group databases

MEROPSM03.002.

PTM databases

PhosphoSiteQ9BYT8.

Polymorphism databases

DMDM20139130.

Proteomic databases

PaxDbQ9BYT8.
PeptideAtlasQ9BYT8.
PRIDEQ9BYT8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000380985; ENSP00000370372; ENSG00000123213.
GeneID57486.
KEGGhsa:57486.
UCSCuc003juf.3. human.

Organism-specific databases

CTD57486.
GeneCardsGC05P065018.
HGNCHGNC:16058. NLN.
HPAHPA031862.
MIM611530. gene.
neXtProtNX_Q9BYT8.
PharmGKBPA31651.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0339.
HOGENOMHOG000245985.
HOVERGENHBG000238.
InParanoidQ9BYT8.
KOK01393.
OMALKETNNY.
OrthoDBEOG7SR4KW.
PhylomeDBQ9BYT8.
TreeFamTF300459.

Gene expression databases

ArrayExpressQ9BYT8.
BgeeQ9BYT8.
CleanExHS_NLN.
GenevestigatorQ9BYT8.

Family and domain databases

Gene3D1.10.1370.10. 2 hits.
1.20.1050.40. 1 hit.
3.40.390.10. 1 hit.
InterProIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiNLN_(gene).
GenomeRNAi57486.
NextBio63764.
PROQ9BYT8.
SOURCESearch...

Entry information

Entry nameNEUL_HUMAN
AccessionPrimary (citable) accession number: Q9BYT8
Secondary accession number(s): Q9ULJ4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: June 1, 2001
Last modified: March 19, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM