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Q9BYT8

- NEUL_HUMAN

UniProt

Q9BYT8 - NEUL_HUMAN

Protein

Neurolysin, mitochondrial

Gene

NLN

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A.By similarity

    Catalytic activityi

    Preferential cleavage in neurotensin: 10-Pro-|-Tyr-11.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi497 – 4971Zinc; catalyticPROSITE-ProRule annotation
    Active sitei498 – 4981PROSITE-ProRule annotation
    Metal bindingi501 – 5011Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi504 – 5041Zinc; catalyticPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. metalloendopeptidase activity Source: InterPro
    3. peptide binding Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM03.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neurolysin, mitochondrial (EC:3.4.24.16)
    Alternative name(s):
    Angiotensin-binding protein
    Microsomal endopeptidase
    Short name:
    MEP
    Mitochondrial oligopeptidase M
    Neurotensin endopeptidase
    Gene namesi
    Name:NLN
    Synonyms:AGTBP, KIAA1226
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:16058. NLN.

    Subcellular locationi

    Mitochondrion intermembrane space By similarity. Cytoplasm By similarity

    GO - Cellular componenti

    1. mitochondrial intermembrane space Source: UniProtKB-SubCell
    2. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA31651.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3737MitochondrionBy similarityAdd
    BLAST
    Chaini38 – 704667Neurolysin, mitochondrialPRO_0000028575Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei664 – 6641N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ9BYT8.
    PaxDbiQ9BYT8.
    PeptideAtlasiQ9BYT8.
    PRIDEiQ9BYT8.

    PTM databases

    PhosphoSiteiQ9BYT8.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9BYT8.
    BgeeiQ9BYT8.
    CleanExiHS_NLN.
    GenevestigatoriQ9BYT8.

    Organism-specific databases

    HPAiHPA031862.

    Interactioni

    Protein-protein interaction databases

    BioGridi121555. 3 interactions.
    IntActiQ9BYT8. 1 interaction.
    STRINGi9606.ENSP00000370372.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BYT8.
    SMRiQ9BYT8. Positions 37-701.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M3 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0339.
    HOGENOMiHOG000245985.
    HOVERGENiHBG000238.
    InParanoidiQ9BYT8.
    KOiK01393.
    OMAiYENCLQV.
    OrthoDBiEOG7SR4KW.
    PhylomeDBiQ9BYT8.
    TreeFamiTF300459.

    Family and domain databases

    Gene3Di1.10.1370.10. 2 hits.
    1.20.1050.40. 1 hit.
    3.40.390.10. 1 hit.
    InterProiIPR024079. MetalloPept_cat_dom.
    IPR024077. Neurolysin/TOP_dom2.
    IPR024080. Neurolysin/TOP_N.
    IPR001567. Pept_M3A_M3B.
    [Graphical view]
    PfamiPF01432. Peptidase_M3. 1 hit.
    [Graphical view]
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9BYT8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIARCLLAVR SLRRVGGSRI LLRMTLGREV MSPLQAMSSY TVAGRNVLRW    50
    DLSPEQIKTR TEELIVQTKQ VYDAVGMLGI EEVTYENCLQ ALADVEVKYI 100
    VERTMLDFPQ HVSSDKEVRA ASTEADKRLS RFDIEMSMRG DIFERIVHLQ 150
    ETCDLGKIKP EARRYLEKSI KMGKRNGLHL PEQVQNEIKS MKKRMSELCI 200
    DFNKNLNEDD TFLVFSKAEL GALPDDFIDS LEKTDDDKYK ITLKYPHYFP 250
    VMKKCCIPET RRRMEMAFNT RCKEENTIIL QQLLPLRTKV AKLLGYSTHA 300
    DFVLEMNTAK STSRVTAFLD DLSQKLKPLG EAEREFILNL KKKECKDRGF 350
    EYDGKINAWD LYYYMTQTEE LKYSIDQEFL KEYFPIEVVT EGLLNTYQEL 400
    LGLSFEQMTD AHVWNKSVTL YTVKDKATGE VLGQFYLDLY PREGKYNHAA 450
    CFGLQPGCLL PDGSRMMAVA ALVVNFSQPV AGRPSLLRHD EVRTYFHEFG 500
    HVMHQICAQT DFARFSGTNV ETDFVEVPSQ MLENWVWDVD SLRRLSKHYK 550
    DGSPIADDLL EKLVASRLVN TGLLTLRQIV LSKVDQSLHT NTSLDAASEY 600
    AKYCSEILGV AATPGTNMPA TFGHLAGGYD GQYYGYLWSE VFSMDMFYSC 650
    FKKEGIMNPE VGMKYRNLIL KPGGSLDGMD MLHNFLKREP NQKAFLMSRG 700
    LHAP 704
    Length:704
    Mass (Da):80,652
    Last modified:June 1, 2001 - v1
    Checksum:i80136688D79BBEDF
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti79 – 791G → S.
    Corresponds to variant rs34339013 [ dbSNP | Ensembl ].
    VAR_062224
    Natural varianti323 – 3231S → G.
    Corresponds to variant rs34063558 [ dbSNP | Ensembl ].
    VAR_054002
    Natural varianti372 – 3721K → R.
    Corresponds to variant rs6863012 [ dbSNP | Ensembl ].
    VAR_054003
    Natural varianti417 – 4171S → G.
    Corresponds to variant rs2289884 [ dbSNP | Ensembl ].
    VAR_054004
    Natural varianti704 – 7041P → S.
    Corresponds to variant rs6860508 [ dbSNP | Ensembl ].
    VAR_024594

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ300837 mRNA. Translation: CAC27329.1.
    AB033052 mRNA. Translation: BAA86540.2.
    CCDSiCCDS3989.1.
    RefSeqiNP_065777.1. NM_020726.4.
    UniGeneiHs.247460.

    Genome annotation databases

    EnsembliENST00000380985; ENSP00000370372; ENSG00000123213.
    GeneIDi57486.
    KEGGihsa:57486.
    UCSCiuc003juf.3. human.

    Polymorphism databases

    DMDMi20139130.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ300837 mRNA. Translation: CAC27329.1 .
    AB033052 mRNA. Translation: BAA86540.2 .
    CCDSi CCDS3989.1.
    RefSeqi NP_065777.1. NM_020726.4.
    UniGenei Hs.247460.

    3D structure databases

    ProteinModelPortali Q9BYT8.
    SMRi Q9BYT8. Positions 37-701.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 121555. 3 interactions.
    IntActi Q9BYT8. 1 interaction.
    STRINGi 9606.ENSP00000370372.

    Protein family/group databases

    MEROPSi M03.002.

    PTM databases

    PhosphoSitei Q9BYT8.

    Polymorphism databases

    DMDMi 20139130.

    Proteomic databases

    MaxQBi Q9BYT8.
    PaxDbi Q9BYT8.
    PeptideAtlasi Q9BYT8.
    PRIDEi Q9BYT8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000380985 ; ENSP00000370372 ; ENSG00000123213 .
    GeneIDi 57486.
    KEGGi hsa:57486.
    UCSCi uc003juf.3. human.

    Organism-specific databases

    CTDi 57486.
    GeneCardsi GC05P065018.
    HGNCi HGNC:16058. NLN.
    HPAi HPA031862.
    MIMi 611530. gene.
    neXtProti NX_Q9BYT8.
    PharmGKBi PA31651.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0339.
    HOGENOMi HOG000245985.
    HOVERGENi HBG000238.
    InParanoidi Q9BYT8.
    KOi K01393.
    OMAi YENCLQV.
    OrthoDBi EOG7SR4KW.
    PhylomeDBi Q9BYT8.
    TreeFami TF300459.

    Miscellaneous databases

    GeneWikii NLN_(gene).
    GenomeRNAii 57486.
    NextBioi 63764.
    PROi Q9BYT8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9BYT8.
    Bgeei Q9BYT8.
    CleanExi HS_NLN.
    Genevestigatori Q9BYT8.

    Family and domain databases

    Gene3Di 1.10.1370.10. 2 hits.
    1.20.1050.40. 1 hit.
    3.40.390.10. 1 hit.
    InterProi IPR024079. MetalloPept_cat_dom.
    IPR024077. Neurolysin/TOP_dom2.
    IPR024080. Neurolysin/TOP_N.
    IPR001567. Pept_M3A_M3B.
    [Graphical view ]
    Pfami PF01432. Peptidase_M3. 1 hit.
    [Graphical view ]
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of human neurolysin, an oligopeptidase of family M3."
      Chen J.M., Rawlings N.D., Barrett A.J.
      Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O.
      DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
      Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
      DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION.
    4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    5. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNEUL_HUMAN
    AccessioniPrimary (citable) accession number: Q9BYT8
    Secondary accession number(s): Q9ULJ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Peptidase families
      Classification of peptidase families and list of entries
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3