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Protein

Serine/threonine-protein kinase 33

Gene

STK33

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase which phosphorylates VIME. May play a specific role in the dynamic behavior of the intermediate filament cytoskeleton by phosphorylation of VIME (By similarity). Not essential for the survival of KRAS-dependent AML cell lines.By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei145 – 1451ATPPROSITE-ProRule annotation
Active sitei238 – 2381Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi122 – 1309ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. protein autophosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ9BYT3.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 33 (EC:2.7.11.1)
Gene namesi
Name:STK33
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:14568. STK33.

Subcellular locationi

  1. Cytoplasmperinuclear region By similarity

GO - Cellular componenti

  1. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37900.

Polymorphism and mutation databases

BioMutaiSTK33.
DMDMi74761329.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 514514Serine/threonine-protein kinase 33PRO_0000232644Add
BLAST

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9BYT3.
PaxDbiQ9BYT3.
PRIDEiQ9BYT3.

PTM databases

PhosphoSiteiQ9BYT3.

Expressioni

Tissue specificityi

Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system.2 Publications

Gene expression databases

BgeeiQ9BYT3.
CleanExiHS_STK33.
ExpressionAtlasiQ9BYT3. baseline and differential.
GenevestigatoriQ9BYT3.

Organism-specific databases

HPAiHPA015742.
HPA056855.

Interactioni

Subunit structurei

Interacts with VIME.By similarity

Protein-protein interaction databases

BioGridi122426. 11 interactions.
IntActiQ9BYT3. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9BYT3.
SMRiQ9BYT3. Positions 78-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini116 – 381266Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi443 – 4464Poly-Glu

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00780000121878.
HOGENOMiHOG000293198.
HOVERGENiHBG094004.
InParanoidiQ9BYT3.
KOiK08813.
OMAiENETRWI.
OrthoDBiEOG712TWR.
PhylomeDBiQ9BYT3.
TreeFamiTF314166.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BYT3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADSGLDKKS TKCPDCSSAS QKDVLCVCSS KTRVPPVLVV EMSQTSSIGS
60 70 80 90 100
AESLISLERK KEKNINRDIT SRKDLPSRTS NVERKASQQQ WGRGNFTEGK
110 120 130 140 150
VPHIRIENGA AIEEIYTFGR ILGKGSFGIV IEATDKETET KWAIKKVNKE
160 170 180 190 200
KAGSSAVKLL EREVNILKSV KHEHIIHLEQ VFETPKKMYL VMELCEDGEL
210 220 230 240 250
KEILDRKGHF SENETRWIIQ SLASAIAYLH NNDIVHRDLK LENIMVKSSL
260 270 280 290 300
IDDNNEINLN IKVTDFGLAV KKQSRSEAML QATCGTPIYM APEVISAHDY
310 320 330 340 350
SQQCDIWSIG VVMYMLLRGE PPFLASSEEK LFELIRKGEL HFENAVWNSI
360 370 380 390 400
SDCAKSVLKQ LMKVDPAHRI TAKELLDNQW LTGNKLSSVR PTNVLEMMKE
410 420 430 440 450
WKNNPESVEE NTTEEKNKPS TEEKLKSYQP WGNVPDANYT SDEEEEKQST
460 470 480 490 500
AYEKQFPATS KDNFDMCSSS FTSSKLLPAE IKGEMEKTPV TPSQGTATKY
510
PAKSGALSRT KKKL
Length:514
Mass (Da):57,831
Last modified:June 1, 2001 - v1
Checksum:iC251691ECB2DAC1A
GO
Isoform 2 (identifier: Q9BYT3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     383-448: Missing.

Note: No experimental confirmation available.

Show »
Length:448
Mass (Da):50,176
Checksum:iFA0DE1B89F81956A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti436 – 4372DA → ET in AAH31231 (PubMed:15489334).Curated
Sequence conflicti451 – 4511A → T in AAH31231 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti60 – 601K → E.
Corresponds to variant rs60786172 [ dbSNP | Ensembl ].
VAR_061746
Natural varianti98 – 981E → D.1 Publication
Corresponds to variant rs34525052 [ dbSNP | Ensembl ].
VAR_041172
Natural varianti160 – 1601L → V in a lung large cell carcinoma sample; somatic mutation. 1 Publication
VAR_041173
Natural varianti436 – 4361D → E.1 Publication
Corresponds to variant rs3751096 [ dbSNP | Ensembl ].
VAR_041174
Natural varianti437 – 4371A → T.1 Publication
Corresponds to variant rs3751095 [ dbSNP | Ensembl ].
VAR_041175
Natural varianti458 – 4581A → E.1 Publication
Corresponds to variant rs35296353 [ dbSNP | Ensembl ].
VAR_041176

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei383 – 44866Missing in isoform 2. 1 PublicationVSP_017939Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303380 mRNA. Translation: CAC29064.1.
AK093251 mRNA. Translation: BAC04109.1.
BC031231 mRNA. Translation: AAH31231.1.
AL833011 mRNA. Translation: CAH56302.1.
CCDSiCCDS7789.1. [Q9BYT3-1]
RefSeqiNP_001275987.1. NM_001289058.1.
NP_001275988.1. NM_001289059.1.
NP_001275990.1. NM_001289061.1. [Q9BYT3-1]
NP_112168.1. NM_030906.3. [Q9BYT3-1]
UniGeneiHs.501833.

Genome annotation databases

EnsembliENST00000315204; ENSP00000320754; ENSG00000130413. [Q9BYT3-1]
ENST00000396672; ENSP00000379905; ENSG00000130413. [Q9BYT3-1]
ENST00000447869; ENSP00000416750; ENSG00000130413. [Q9BYT3-1]
GeneIDi65975.
KEGGihsa:65975.
UCSCiuc001mgi.1. human. [Q9BYT3-1]

Polymorphism and mutation databases

BioMutaiSTK33.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303380 mRNA. Translation: CAC29064.1.
AK093251 mRNA. Translation: BAC04109.1.
BC031231 mRNA. Translation: AAH31231.1.
AL833011 mRNA. Translation: CAH56302.1.
CCDSiCCDS7789.1. [Q9BYT3-1]
RefSeqiNP_001275987.1. NM_001289058.1.
NP_001275988.1. NM_001289059.1.
NP_001275990.1. NM_001289061.1. [Q9BYT3-1]
NP_112168.1. NM_030906.3. [Q9BYT3-1]
UniGeneiHs.501833.

3D structure databases

ProteinModelPortaliQ9BYT3.
SMRiQ9BYT3. Positions 78-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122426. 11 interactions.
IntActiQ9BYT3. 4 interactions.

Chemistry

BindingDBiQ9BYT3.
ChEMBLiCHEMBL6005.
GuidetoPHARMACOLOGYi2221.

PTM databases

PhosphoSiteiQ9BYT3.

Polymorphism and mutation databases

BioMutaiSTK33.
DMDMi74761329.

Proteomic databases

MaxQBiQ9BYT3.
PaxDbiQ9BYT3.
PRIDEiQ9BYT3.

Protocols and materials databases

DNASUi65975.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000315204; ENSP00000320754; ENSG00000130413. [Q9BYT3-1]
ENST00000396672; ENSP00000379905; ENSG00000130413. [Q9BYT3-1]
ENST00000447869; ENSP00000416750; ENSG00000130413. [Q9BYT3-1]
GeneIDi65975.
KEGGihsa:65975.
UCSCiuc001mgi.1. human. [Q9BYT3-1]

Organism-specific databases

CTDi65975.
GeneCardsiGC11M008369.
HGNCiHGNC:14568. STK33.
HPAiHPA015742.
HPA056855.
MIMi607670. gene.
neXtProtiNX_Q9BYT3.
PharmGKBiPA37900.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00780000121878.
HOGENOMiHOG000293198.
HOVERGENiHBG094004.
InParanoidiQ9BYT3.
KOiK08813.
OMAiENETRWI.
OrthoDBiEOG712TWR.
PhylomeDBiQ9BYT3.
TreeFamiTF314166.

Enzyme and pathway databases

SignaLinkiQ9BYT3.

Miscellaneous databases

GenomeRNAii65975.
NextBioi67414.
PROiQ9BYT3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BYT3.
CleanExiHS_STK33.
ExpressionAtlasiQ9BYT3. baseline and differential.
GenevestigatoriQ9BYT3.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel serine/threonine kinase gene, STK33, on human chromosome 11p15.3."
    Mujica A.O., Hankeln T., Schmidt E.R.
    Gene 280:175-181(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Uterus.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 246-514 (ISOFORM 2).
    Tissue: Stomach.
  5. "Differential expression pattern of the novel serine/threonine kinase, STK33, in mice and men."
    Mujica A.O., Brauksiepe B., Saaler-Reinhardt S., Reuss S., Schmidt E.R.
    FEBS J. 272:4884-4898(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: FUNCTION.
  9. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS [LARGE SCALE ANALYSIS] ASP-98; VAL-160; GLU-436; THR-437 AND GLU-458.

Entry informationi

Entry nameiSTK33_HUMAN
AccessioniPrimary (citable) accession number: Q9BYT3
Secondary accession number(s): Q658S6, Q8NEF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: June 1, 2001
Last modified: April 29, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.