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Q9BYT3 (STK33_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase 33

EC=2.7.11.1
Gene names
Name:STK33
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine/threonine protein kinase which phosphorylates VIME. May play a specific role in the dynamic behavior of the intermediate filament cytoskeleton by phosphorylation of VIME By similarity. Not essential for the survival of KRAS-dependent AML cell lines. Ref.8

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with VIME By similarity.

Subcellular location

Cytoplasmperinuclear region By similarity.

Tissue specificity

Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system. Ref.1 Ref.5

Post-translational modification

Autophosphorylated By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Contains 1 protein kinase domain.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9BYT3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9BYT3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     383-448: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 514514Serine/threonine-protein kinase 33
PRO_0000232644

Regions

Domain116 – 381266Protein kinase
Nucleotide binding122 – 1309ATP By similarity
Compositional bias443 – 4464Poly-Glu

Sites

Active site2381Proton acceptor By similarity
Binding site1451ATP By similarity

Natural variations

Alternative sequence383 – 44866Missing in isoform 2.
VSP_017939
Natural variant601K → E.
Corresponds to variant rs60786172 [ dbSNP | Ensembl ].
VAR_061746
Natural variant981E → D. Ref.9
Corresponds to variant rs34525052 [ dbSNP | Ensembl ].
VAR_041172
Natural variant1601L → V in a lung large cell carcinoma sample; somatic mutation. Ref.9
VAR_041173
Natural variant4361D → E. Ref.9
Corresponds to variant rs3751096 [ dbSNP | Ensembl ].
VAR_041174
Natural variant4371A → T. Ref.9
Corresponds to variant rs3751095 [ dbSNP | Ensembl ].
VAR_041175
Natural variant4581A → E. Ref.9
Corresponds to variant rs35296353 [ dbSNP | Ensembl ].
VAR_041176

Experimental info

Sequence conflict436 – 4372DA → ET in AAH31231. Ref.3
Sequence conflict4511A → T in AAH31231. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: C251691ECB2DAC1A

FASTA51457,831
        10         20         30         40         50         60 
MADSGLDKKS TKCPDCSSAS QKDVLCVCSS KTRVPPVLVV EMSQTSSIGS AESLISLERK 

        70         80         90        100        110        120 
KEKNINRDIT SRKDLPSRTS NVERKASQQQ WGRGNFTEGK VPHIRIENGA AIEEIYTFGR 

       130        140        150        160        170        180 
ILGKGSFGIV IEATDKETET KWAIKKVNKE KAGSSAVKLL EREVNILKSV KHEHIIHLEQ 

       190        200        210        220        230        240 
VFETPKKMYL VMELCEDGEL KEILDRKGHF SENETRWIIQ SLASAIAYLH NNDIVHRDLK 

       250        260        270        280        290        300 
LENIMVKSSL IDDNNEINLN IKVTDFGLAV KKQSRSEAML QATCGTPIYM APEVISAHDY 

       310        320        330        340        350        360 
SQQCDIWSIG VVMYMLLRGE PPFLASSEEK LFELIRKGEL HFENAVWNSI SDCAKSVLKQ 

       370        380        390        400        410        420 
LMKVDPAHRI TAKELLDNQW LTGNKLSSVR PTNVLEMMKE WKNNPESVEE NTTEEKNKPS 

       430        440        450        460        470        480 
TEEKLKSYQP WGNVPDANYT SDEEEEKQST AYEKQFPATS KDNFDMCSSS FTSSKLLPAE 

       490        500        510 
IKGEMEKTPV TPSQGTATKY PAKSGALSRT KKKL 

« Hide

Isoform 2 [UniParc].

Checksum: FA0DE1B89F81956A
Show »

FASTA44850,176

References

« Hide 'large scale' references
[1]"A novel serine/threonine kinase gene, STK33, on human chromosome 11p15.3."
Mujica A.O., Hankeln T., Schmidt E.R.
Gene 280:175-181(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Uterus.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 246-514 (ISOFORM 2).
Tissue: Stomach.
[5]"Differential expression pattern of the novel serine/threonine kinase, STK33, in mice and men."
Mujica A.O., Brauksiepe B., Saaler-Reinhardt S., Reuss S., Schmidt E.R.
FEBS J. 272:4884-4898(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"STk33 kinase activity is non-essential in KRAS-dependent cancer cells."
Babij C., Zhang Y., Kurzeja R.J., Munzli A., Shehabeldin A., Fernando M., Quon K., Kassner P.D., Ruefli-Brasse A.A., Watson V.J., Fajardo F., Jackson A., Zondlo J., Sun Y., Ellison A.R., Plewa C.A., San Miguel T., Robinson J. expand/collapse author list , McCarter J., Schwandner R., Judd T., Carnahan J., Dussault I.
Cancer Res. 71:5818-5826(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] ASP-98; VAL-160; GLU-436; THR-437 AND GLU-458.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ303380 mRNA. Translation: CAC29064.1.
AK093251 mRNA. Translation: BAC04109.1.
BC031231 mRNA. Translation: AAH31231.1.
AL833011 mRNA. Translation: CAH56302.1.
CCDSCCDS7789.1. [Q9BYT3-1]
RefSeqNP_001275987.1. NM_001289058.1.
NP_001275988.1. NM_001289059.1.
NP_001275990.1. NM_001289061.1. [Q9BYT3-1]
NP_112168.1. NM_030906.3. [Q9BYT3-1]
XP_006718342.1. XM_006718279.1. [Q9BYT3-2]
UniGeneHs.501833.

3D structure databases

ProteinModelPortalQ9BYT3.
SMRQ9BYT3. Positions 78-441.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122426. 11 interactions.
IntActQ9BYT3. 2 interactions.

Chemistry

BindingDBQ9BYT3.
ChEMBLCHEMBL6005.
GuidetoPHARMACOLOGY2221.

PTM databases

PhosphoSiteQ9BYT3.

Polymorphism databases

DMDM74761329.

Proteomic databases

MaxQBQ9BYT3.
PaxDbQ9BYT3.
PRIDEQ9BYT3.

Protocols and materials databases

DNASU65975.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000315204; ENSP00000320754; ENSG00000130413. [Q9BYT3-1]
ENST00000396672; ENSP00000379905; ENSG00000130413. [Q9BYT3-1]
ENST00000396673; ENSP00000379906; ENSG00000130413. [Q9BYT3-2]
ENST00000444064; ENSP00000415688; ENSG00000130413.
ENST00000447869; ENSP00000416750; ENSG00000130413. [Q9BYT3-1]
GeneID65975.
KEGGhsa:65975.
UCSCuc001mgi.1. human. [Q9BYT3-1]

Organism-specific databases

CTD65975.
GeneCardsGC11M008369.
HGNCHGNC:14568. STK33.
HPAHPA015742.
MIM607670. gene.
neXtProtNX_Q9BYT3.
PharmGKBPA37900.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000293198.
HOVERGENHBG094004.
InParanoidQ9BYT3.
KOK08813.
OMAKKMYLVM.
OrthoDBEOG712TWR.
PhylomeDBQ9BYT3.
TreeFamTF314166.

Enzyme and pathway databases

SignaLinkQ9BYT3.

Gene expression databases

ArrayExpressQ9BYT3.
BgeeQ9BYT3.
CleanExHS_STK33.
GenevestigatorQ9BYT3.

Family and domain databases

InterProIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERPTHR24347. PTHR24347. 1 hit.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi65975.
NextBio67414.
PROQ9BYT3.
SOURCESearch...

Entry information

Entry nameSTK33_HUMAN
AccessionPrimary (citable) accession number: Q9BYT3
Secondary accession number(s): Q658S6, Q8NEF5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM