Q9BYT3 (STK33_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase 33
EC=2.7.11.1

Gene names

Name:

STK33

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine/threonine protein kinase which phosphorylates VIME. May play a specific role in the dynamic behavior of the intermediate filament cytoskeleton by phosphorylation of VIME By similarity. Not essential for the survival of KRAS-dependent AML cell lines. Ref.8
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with VIME By similarity.
Subcellular location	Cytoplasm › perinuclear region By similarity.
Tissue specificity	Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system. Ref.1 Ref.5
Post-translational modification	Autophosphorylated By similarity. Ref.6 Ref.7
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein autophosphorylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	Golgi apparatus Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BYT3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BYT3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     383-448: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 514	514	Serine/threonine-protein kinase 33		PRO_0000232644
Regions
	Domain	116 – 381	266	Protein kinase
	Nucleotide binding	122 – 130	9	ATP By similarity
	Compositional bias	443 – 446	4	Poly-Glu
Sites
	Active site	238	1	Proton acceptor By similarity
	Binding site	145	1	ATP By similarity
Amino acid modifications
	Modified residue	441	1	Phosphoserine Ref.7
	Modified residue	469	1	Phosphoserine Ref.6
	Modified residue	470	1	Phosphoserine Ref.6
	Modified residue	491	1	Phosphothreonine Ref.7
Natural variations
	Alternative sequence	383 – 448	66	Missing in isoform 2.		VSP_017939
	Natural variant	60	1	K → E. Corresponds to variant rs60786172 [ dbSNP | Ensembl ].		VAR_061746
	Natural variant	98	1	E → D. Ref.9 Corresponds to variant rs34525052 [ dbSNP | Ensembl ].		VAR_041172
	Natural variant	160	1	L → V in a lung large cell carcinoma sample; somatic mutation. Ref.9		VAR_041173
	Natural variant	436	1	D → E. Ref.9 Corresponds to variant rs3751096 [ dbSNP | Ensembl ].		VAR_041174
	Natural variant	437	1	A → T. Ref.9 Corresponds to variant rs3751095 [ dbSNP | Ensembl ].		VAR_041175
	Natural variant	458	1	A → E. Ref.9 Corresponds to variant rs35296353 [ dbSNP | Ensembl ].		VAR_041176
Experimental info
	Sequence conflict	436 – 437	2	DA → ET in AAH31231. Ref.3
	Sequence conflict	451	1	A → T in AAH31231. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: C251691ECB2DAC1A	FASTA	514	57,831
	10 20 30 40 50 60 MADSGLDKKS TKCPDCSSAS QKDVLCVCSS KTRVPPVLVV EMSQTSSIGS AESLISLERK 70 80 90 100 110 120 KEKNINRDIT SRKDLPSRTS NVERKASQQQ WGRGNFTEGK VPHIRIENGA AIEEIYTFGR 130 140 150 160 170 180 ILGKGSFGIV IEATDKETET KWAIKKVNKE KAGSSAVKLL EREVNILKSV KHEHIIHLEQ 190 200 210 220 230 240 VFETPKKMYL VMELCEDGEL KEILDRKGHF SENETRWIIQ SLASAIAYLH NNDIVHRDLK 250 260 270 280 290 300 LENIMVKSSL IDDNNEINLN IKVTDFGLAV KKQSRSEAML QATCGTPIYM APEVISAHDY 310 320 330 340 350 360 SQQCDIWSIG VVMYMLLRGE PPFLASSEEK LFELIRKGEL HFENAVWNSI SDCAKSVLKQ 370 380 390 400 410 420 LMKVDPAHRI TAKELLDNQW LTGNKLSSVR PTNVLEMMKE WKNNPESVEE NTTEEKNKPS 430 440 450 460 470 480 TEEKLKSYQP WGNVPDANYT SDEEEEKQST AYEKQFPATS KDNFDMCSSS FTSSKLLPAE 490 500 510 IKGEMEKTPV TPSQGTATKY PAKSGALSRT KKKL
	Isoform 2 [UniParc]. Checksum: FA0DE1B89F81956A	FASTA	448	50,176
	10 20 30 40 50 60 MADSGLDKKS TKCPDCSSAS QKDVLCVCSS KTRVPPVLVV EMSQTSSIGS AESLISLERK 70 80 90 100 110 120 KEKNINRDIT SRKDLPSRTS NVERKASQQQ WGRGNFTEGK VPHIRIENGA AIEEIYTFGR 130 140 150 160 170 180 ILGKGSFGIV IEATDKETET KWAIKKVNKE KAGSSAVKLL EREVNILKSV KHEHIIHLEQ 190 200 210 220 230 240 VFETPKKMYL VMELCEDGEL KEILDRKGHF SENETRWIIQ SLASAIAYLH NNDIVHRDLK 250 260 270 280 290 300 LENIMVKSSL IDDNNEINLN IKVTDFGLAV KKQSRSEAML QATCGTPIYM APEVISAHDY 310 320 330 340 350 360 SQQCDIWSIG VVMYMLLRGE PPFLASSEEK LFELIRKGEL HFENAVWNSI SDCAKSVLKQ 370 380 390 400 410 420 LMKVDPAHRI TAKELLDNQW LTSTAYEKQF PATSKDNFDM CSSSFTSSKL LPAEIKGEME 430 440 KTPVTPSQGT ATKYPAKSGA LSRTKKKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel serine/threonine kinase gene, STK33, on human chromosome 11p15.3." Mujica A.O., Hankeln T., Schmidt E.R. Gene 280:175-181(2001) [PubMed: 11738831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Uterus.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 246-514 (ISOFORM 2). Tissue: Stomach.
[5]	"Differential expression pattern of the novel serine/threonine kinase, STK33, in mice and men." Mujica A.O., Brauksiepe B., Saaler-Reinhardt S., Reuss S., Schmidt E.R. FEBS J. 272:4884-4898(2005) [PubMed: 16176263] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-469 AND SER-470, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-441 AND THR-491, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[8]	"STk33 kinase activity is non-essential in KRAS-dependent cancer cells." Babij C., Zhang Y., Kurzeja R.J., Munzli A., Shehabeldin A., Fernando M., Quon K., Kassner P.D., Ruefli-Brasse A.A., Watson V.J., Fajardo F., Jackson A., Zondlo J., Sun Y., Ellison A.R., Plewa C.A., San Miguel T., Robinson J. , McCarter J., Schwandner R., Judd T., Carnahan J., Dussault I. Cancer Res. 71:5818-5826(2011) [PubMed: 21742770] [Abstract] Cited for: FUNCTION.
[9]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] ASP-98; VAL-160; GLU-436; THR-437 AND GLU-458.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ303380 mRNA. Translation: CAC29064.1. AK093251 mRNA. Translation: BAC04109.1. BC031231 mRNA. Translation: AAH31231.1. AL833011 mRNA. Translation: CAH56302.1.
IPI	IPI00302351. IPI00747557.
RefSeq	NP_112168.1. NM_030906.2.
UniGene	Hs.501833.
3D structure databases
ProteinModelPortal	Q9BYT3.
SMR	Q9BYT3. Positions 111-477.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BYT3. 2 interactions.
STRING	Q9BYT3.
PTM databases
PhosphoSite	Q9BYT3.
Polymorphism databases
DMDM	74761329.
Proteomic databases
PRIDE	Q9BYT3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000315204; ENSP00000320754; ENSG00000130413. ENST00000396672; ENSP00000379905; ENSG00000130413. ENST00000447869; ENSP00000416750; ENSG00000130413.
GeneID	65975.
KEGG	hsa:65975.
UCSC	uc001mgi.1. human.
Organism-specific databases
CTD	65975.
GeneCards	GC11M008369.
H-InvDB	HIX0009423.
HGNC	HGNC:14568. STK33.
HPA	HPA015742.
MIM	607670. gene.
neXtProt	NX_Q9BYT3.
PharmGKB	PA37900.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG06606.
GeneTree	ENSGT00600000084006.
HOGENOM	HBG283285.
HOVERGEN	HBG094004.
InParanoid	Q9BYT3.
OMA	SENETRW.
PhylomeDB	Q9BYT3.
Gene expression databases
ArrayExpress	Q9BYT3.
Bgee	Q9BYT3.
CleanEx	HS_STK33.
Genevestigator	Q9BYT3.
GermOnline	ENSG00000130413. Homo sapiens.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR020645. Ser/Thr_kinase_33. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K08813.
PANTHER	PTHR24347. PTHR24347. 1 hit. PTHR24347:SF20. PTHR24347:SF20. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	67414.
SOURCE	Search...

Entry information

Entry name

STK33_HUMAN

Accession

Primary (citable) accession number: Q9BYT3
Secondary accession number(s): Q658S6, Q8NEF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.