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Reviewed, UniProtKB/Swiss-Prot Q9BYT3 (STK33_HUMAN)

Last modified June 16, 2009. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Serine/threonine-protein kinase 33
    EC=2.7.11.1
Gene names
Name: STK33
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subcellular location

Cytoplasmperinuclear region By similarity.

Tissue specificity

Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system. Ref.1 Ref.5

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological processprotein amino acid phosphorylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentperinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9BYT3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9BYT3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     383-448: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 514514Serine/threonine-protein kinase 33
PRO_0000232644

Regions

Domain116 – 381266Protein kinase
Nucleotide binding122 – 1309ATP By similarity
Compositional bias443 – 4464Poly-Glu

Sites

Active site2381Proton acceptor By similarity
Binding site1451ATP By similarity

Amino acid modifications

Modified residue4691Phosphoserine Ref.6
Modified residue4701Phosphoserine Ref.6

Natural variations

Alternative sequence383 – 44866Missing in isoform 2.
VSP_017939
Natural variant981E → D: dbSNP rs34525052. Ref.7
VAR_041172
Natural variant1601L → V in a lung large cell carcinoma sample; somatic mutation. Ref.7
VAR_041173
Natural variant4361D → E: dbSNP rs3751096. Ref.7
VAR_041174
Natural variant4371A → T: dbSNP rs3751095. Ref.7
VAR_041175
Natural variant4581A → E: dbSNP rs35296353. Ref.7
VAR_041176

Experimental info

Sequence conflict436 – 4372DA → ET in AAH31231. Ref.3
Sequence conflict4511A → T in AAH31231. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: C251691ECB2DAC1A

FASTA51457,831
        10         20         30         40         50         60 
MADSGLDKKS TKCPDCSSAS QKDVLCVCSS KTRVPPVLVV EMSQTSSIGS AESLISLERK 

        70         80         90        100        110        120 
KEKNINRDIT SRKDLPSRTS NVERKASQQQ WGRGNFTEGK VPHIRIENGA AIEEIYTFGR 

       130        140        150        160        170        180 
ILGKGSFGIV IEATDKETET KWAIKKVNKE KAGSSAVKLL EREVNILKSV KHEHIIHLEQ 

       190        200        210        220        230        240 
VFETPKKMYL VMELCEDGEL KEILDRKGHF SENETRWIIQ SLASAIAYLH NNDIVHRDLK 

       250        260        270        280        290        300 
LENIMVKSSL IDDNNEINLN IKVTDFGLAV KKQSRSEAML QATCGTPIYM APEVISAHDY 

       310        320        330        340        350        360 
SQQCDIWSIG VVMYMLLRGE PPFLASSEEK LFELIRKGEL HFENAVWNSI SDCAKSVLKQ 

       370        380        390        400        410        420 
LMKVDPAHRI TAKELLDNQW LTGNKLSSVR PTNVLEMMKE WKNNPESVEE NTTEEKNKPS 

       430        440        450        460        470        480 
TEEKLKSYQP WGNVPDANYT SDEEEEKQST AYEKQFPATS KDNFDMCSSS FTSSKLLPAE 

       490        500        510 
IKGEMEKTPV TPSQGTATKY PAKSGALSRT KKKL 

« Hide

Isoform 2.

Checksum: FA0DE1B89F81956A
Show »

FASTA44850,176

References

« Hide 'large scale' references
[1]"A novel serine/threonine kinase gene, STK33, on human chromosome 11p15.3."
Mujica A.O., Hankeln T., Schmidt E.R.
Gene 280:175-181(2001) [PubMed: 11738831] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Tissue: Uterus.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 246-514 (ISOFORM 2).
Tissue: Stomach.
[5]"Differential expression pattern of the novel serine/threonine kinase, STK33, in mice and men."
Mujica A.O., Brauksiepe B., Saaler-Reinhardt S., Reuss S., Schmidt E.R.
FEBS J. 272:4884-4898(2005) [PubMed: 16176263] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-469 AND SER-470, MASS SPECTROMETRY.
[7]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed: 17344846] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] ASP-98; VAL-160; GLU-436; THR-437 AND GLU-458.

Cross-references

Sequence databases

AJ303380 mRNA. Translation: CAC29064.1.
AK093251 mRNA. Translation: BAC04109.1.
BC031231 mRNA. Translation: AAH31231.1.
AL833011 mRNA. Translation: CAH56302.1.
IPIIPI00302351.
IPI00747557.
RefSeqNP_112168.1.
UniGeneHs.501833

3D structure databases

HSSPHSSP built from PDB template 1NY3 based on UniProtKB P49137.
ModBaseSearch...

PTM databases

PhosphoSiteQ9BYT3.

Proteomic databases

PRIDEQ9BYT3.

Genome annotation databases

EnsemblENSG00000130413. Homo sapiens. [Contig view]
GeneID65975.
KEGGhsa:65975.

Organism-specific databases

GeneCardsGC11M008369.
HGNCHGNC:14568. STK33.
HPAHPA015742.
MIM607670. gene.
PharmGKBPA37900.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ9BYT3.
HOVERGENQ9BYT3.
OMAQ9BYT3. SENETRW.

Enzyme and pathway databases

BRENDA2.7.11.1. 247.

Gene expression databases

ArrayExpressQ9BYT3.
BgeeQ9BYT3.
CleanExHS_STK33.
GermOnlineENSG00000130413. Homo sapiens.

Family and domain databases

InterProIPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio67414.
SOURCESearch...

Entry information

Entry nameSTK33_HUMAN
AccessionPrimary (citable) accession number: Q9BYT3
Secondary accession number(s): Q658S6, Q8NEF5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: June 1, 2001
Last modified: June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents