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Q9BYT3

- STK33_HUMAN

UniProt

Q9BYT3 - STK33_HUMAN

Protein

Serine/threonine-protein kinase 33

Gene

STK33

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Serine/threonine protein kinase which phosphorylates VIME. May play a specific role in the dynamic behavior of the intermediate filament cytoskeleton by phosphorylation of VIME By similarity. Not essential for the survival of KRAS-dependent AML cell lines.By similarity1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei145 – 1451ATPPROSITE-ProRule annotation
    Active sitei238 – 2381Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi122 – 1309ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. protein autophosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SignaLinkiQ9BYT3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase 33 (EC:2.7.11.1)
    Gene namesi
    Name:STK33
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:14568. STK33.

    Subcellular locationi

    Cytoplasmperinuclear region By similarity

    GO - Cellular componenti

    1. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA37900.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 514514Serine/threonine-protein kinase 33PRO_0000232644Add
    BLAST

    Post-translational modificationi

    Autophosphorylated.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9BYT3.
    PaxDbiQ9BYT3.
    PRIDEiQ9BYT3.

    PTM databases

    PhosphoSiteiQ9BYT3.

    Expressioni

    Tissue specificityi

    Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system.2 Publications

    Gene expression databases

    ArrayExpressiQ9BYT3.
    BgeeiQ9BYT3.
    CleanExiHS_STK33.
    GenevestigatoriQ9BYT3.

    Organism-specific databases

    HPAiHPA015742.

    Interactioni

    Subunit structurei

    Interacts with VIME.By similarity

    Protein-protein interaction databases

    BioGridi122426. 11 interactions.
    IntActiQ9BYT3. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BYT3.
    SMRiQ9BYT3. Positions 78-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini116 – 381266Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi443 – 4464Poly-Glu

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000293198.
    HOVERGENiHBG094004.
    InParanoidiQ9BYT3.
    KOiK08813.
    OMAiKKMYLVM.
    OrthoDBiEOG712TWR.
    PhylomeDBiQ9BYT3.
    TreeFamiTF314166.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9BYT3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MADSGLDKKS TKCPDCSSAS QKDVLCVCSS KTRVPPVLVV EMSQTSSIGS    50
    AESLISLERK KEKNINRDIT SRKDLPSRTS NVERKASQQQ WGRGNFTEGK 100
    VPHIRIENGA AIEEIYTFGR ILGKGSFGIV IEATDKETET KWAIKKVNKE 150
    KAGSSAVKLL EREVNILKSV KHEHIIHLEQ VFETPKKMYL VMELCEDGEL 200
    KEILDRKGHF SENETRWIIQ SLASAIAYLH NNDIVHRDLK LENIMVKSSL 250
    IDDNNEINLN IKVTDFGLAV KKQSRSEAML QATCGTPIYM APEVISAHDY 300
    SQQCDIWSIG VVMYMLLRGE PPFLASSEEK LFELIRKGEL HFENAVWNSI 350
    SDCAKSVLKQ LMKVDPAHRI TAKELLDNQW LTGNKLSSVR PTNVLEMMKE 400
    WKNNPESVEE NTTEEKNKPS TEEKLKSYQP WGNVPDANYT SDEEEEKQST 450
    AYEKQFPATS KDNFDMCSSS FTSSKLLPAE IKGEMEKTPV TPSQGTATKY 500
    PAKSGALSRT KKKL 514
    Length:514
    Mass (Da):57,831
    Last modified:June 1, 2001 - v1
    Checksum:iC251691ECB2DAC1A
    GO
    Isoform 2 (identifier: Q9BYT3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         383-448: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:448
    Mass (Da):50,176
    Checksum:iFA0DE1B89F81956A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti436 – 4372DA → ET in AAH31231. (PubMed:15489334)Curated
    Sequence conflicti451 – 4511A → T in AAH31231. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti60 – 601K → E.
    Corresponds to variant rs60786172 [ dbSNP | Ensembl ].
    VAR_061746
    Natural varianti98 – 981E → D.1 Publication
    Corresponds to variant rs34525052 [ dbSNP | Ensembl ].
    VAR_041172
    Natural varianti160 – 1601L → V in a lung large cell carcinoma sample; somatic mutation. 1 Publication
    VAR_041173
    Natural varianti436 – 4361D → E.1 Publication
    Corresponds to variant rs3751096 [ dbSNP | Ensembl ].
    VAR_041174
    Natural varianti437 – 4371A → T.1 Publication
    Corresponds to variant rs3751095 [ dbSNP | Ensembl ].
    VAR_041175
    Natural varianti458 – 4581A → E.1 Publication
    Corresponds to variant rs35296353 [ dbSNP | Ensembl ].
    VAR_041176

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei383 – 44866Missing in isoform 2. 1 PublicationVSP_017939Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ303380 mRNA. Translation: CAC29064.1.
    AK093251 mRNA. Translation: BAC04109.1.
    BC031231 mRNA. Translation: AAH31231.1.
    AL833011 mRNA. Translation: CAH56302.1.
    CCDSiCCDS7789.1. [Q9BYT3-1]
    RefSeqiNP_001275987.1. NM_001289058.1.
    NP_001275988.1. NM_001289059.1.
    NP_001275990.1. NM_001289061.1. [Q9BYT3-1]
    NP_112168.1. NM_030906.3. [Q9BYT3-1]
    XP_006718342.1. XM_006718279.1. [Q9BYT3-2]
    UniGeneiHs.501833.

    Genome annotation databases

    EnsembliENST00000315204; ENSP00000320754; ENSG00000130413. [Q9BYT3-1]
    ENST00000396672; ENSP00000379905; ENSG00000130413. [Q9BYT3-1]
    ENST00000444064; ENSP00000415688; ENSG00000130413.
    ENST00000447869; ENSP00000416750; ENSG00000130413. [Q9BYT3-1]
    GeneIDi65975.
    KEGGihsa:65975.
    UCSCiuc001mgi.1. human. [Q9BYT3-1]

    Polymorphism databases

    DMDMi74761329.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ303380 mRNA. Translation: CAC29064.1 .
    AK093251 mRNA. Translation: BAC04109.1 .
    BC031231 mRNA. Translation: AAH31231.1 .
    AL833011 mRNA. Translation: CAH56302.1 .
    CCDSi CCDS7789.1. [Q9BYT3-1 ]
    RefSeqi NP_001275987.1. NM_001289058.1.
    NP_001275988.1. NM_001289059.1.
    NP_001275990.1. NM_001289061.1. [Q9BYT3-1 ]
    NP_112168.1. NM_030906.3. [Q9BYT3-1 ]
    XP_006718342.1. XM_006718279.1. [Q9BYT3-2 ]
    UniGenei Hs.501833.

    3D structure databases

    ProteinModelPortali Q9BYT3.
    SMRi Q9BYT3. Positions 78-441.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122426. 11 interactions.
    IntActi Q9BYT3. 4 interactions.

    Chemistry

    BindingDBi Q9BYT3.
    ChEMBLi CHEMBL6005.
    GuidetoPHARMACOLOGYi 2221.

    PTM databases

    PhosphoSitei Q9BYT3.

    Polymorphism databases

    DMDMi 74761329.

    Proteomic databases

    MaxQBi Q9BYT3.
    PaxDbi Q9BYT3.
    PRIDEi Q9BYT3.

    Protocols and materials databases

    DNASUi 65975.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000315204 ; ENSP00000320754 ; ENSG00000130413 . [Q9BYT3-1 ]
    ENST00000396672 ; ENSP00000379905 ; ENSG00000130413 . [Q9BYT3-1 ]
    ENST00000444064 ; ENSP00000415688 ; ENSG00000130413 .
    ENST00000447869 ; ENSP00000416750 ; ENSG00000130413 . [Q9BYT3-1 ]
    GeneIDi 65975.
    KEGGi hsa:65975.
    UCSCi uc001mgi.1. human. [Q9BYT3-1 ]

    Organism-specific databases

    CTDi 65975.
    GeneCardsi GC11M008369.
    HGNCi HGNC:14568. STK33.
    HPAi HPA015742.
    MIMi 607670. gene.
    neXtProti NX_Q9BYT3.
    PharmGKBi PA37900.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000293198.
    HOVERGENi HBG094004.
    InParanoidi Q9BYT3.
    KOi K08813.
    OMAi KKMYLVM.
    OrthoDBi EOG712TWR.
    PhylomeDBi Q9BYT3.
    TreeFami TF314166.

    Enzyme and pathway databases

    SignaLinki Q9BYT3.

    Miscellaneous databases

    GenomeRNAii 65975.
    NextBioi 67414.
    PROi Q9BYT3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9BYT3.
    Bgeei Q9BYT3.
    CleanExi HS_STK33.
    Genevestigatori Q9BYT3.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel serine/threonine kinase gene, STK33, on human chromosome 11p15.3."
      Mujica A.O., Hankeln T., Schmidt E.R.
      Gene 280:175-181(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Tissue: Uterus.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Testis.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 246-514 (ISOFORM 2).
      Tissue: Stomach.
    5. "Differential expression pattern of the novel serine/threonine kinase, STK33, in mice and men."
      Mujica A.O., Brauksiepe B., Saaler-Reinhardt S., Reuss S., Schmidt E.R.
      FEBS J. 272:4884-4898(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: FUNCTION.
    9. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS [LARGE SCALE ANALYSIS] ASP-98; VAL-160; GLU-436; THR-437 AND GLU-458.

    Entry informationi

    Entry nameiSTK33_HUMAN
    AccessioniPrimary (citable) accession number: Q9BYT3
    Secondary accession number(s): Q658S6, Q8NEF5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2006
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3