Q9BYG5 (PAR6B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Partitioning defective 6 homolog beta
Short name=PAR-6 beta
Short name=PAR-6B

Gene names

Name:	PARD6B
Synonyms:	PAR6B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
Subunit structure	Interacts with PARD3. Interacts with GTP-bound forms of CDC42 and RAC1. Interacts with GTP-bound ARHQ/TC10. Interacts with MPP5 By similarity. Interacts with the N-terminal part of PRKCI and PRKCZ. Part of a complex with PARD3, CDC42 or RAC1 and PRKCI or PRKCZ. Part of a complex with LLGL1 and PRKCI. Interacts with ALS2CR19. Interacts with ECT2. Ref.1 Ref.4 Ref.5 Ref.6
Subcellular location	Cytoplasm. Cell membrane By similarity. Cell junction › tight junction By similarity.
Tissue specificity	Expressed in pancreas and in both adult and fetal kidney. Weakly expressed in placenta and lung. Not expressed in other tissues.
Domain	The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases. The PDZ domain mediates interaction with MPP5 By similarity.
Sequence similarities	Belongs to the PAR6 family. Contains 1 OPR domain. Contains 1 PDZ (DHR) domain. Contains 1 pseudo-CRIB domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cell junction Cell membrane Cytoplasm Membrane Tight junction
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axonogenesis Traceable author statement PubMed 14676191. Source: UniProtKB cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW establishment or maintenance of cell polarity Traceable author statement PubMed 14676191. Source: UniProtKB protein complex assembly Inferred from direct assay PubMed 12545177. Source: UniProtKB regulation of cell migration Traceable author statement PubMed 14676191. Source: UniProtKB tight junction assembly Traceable author statement. Source: Reactome
Cellular_component	apical part of cell Inferred from electronic annotation. Source: Compara cell cortex Inferred from electronic annotation. Source: Compara cytosol Traceable author statement. Source: Reactome nucleus Inferred from electronic annotation. Source: Compara plasma membrane Inferred from direct assay PubMed 12545177. Source: UniProtKB protein complex Inferred from electronic annotation. Source: Compara tight junction Inferred from direct assay PubMed 12545177. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC42	P60953	7	EBI-295391,EBI-81752
PARD3	Q8TEW0	4	EBI-295391,EBI-81968
PNMA1	Q8ND90	2	EBI-295391,EBI-302345
PRKCI	P41743	9	EBI-295391,EBI-286199
PRKCZ	Q05513	3	EBI-295391,EBI-295351
RAC1	P63000	3	EBI-295391,EBI-413628
YWHAH	Q04917	2	EBI-295391,EBI-306940

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Partitioning defective 6 homolog beta		PRO_0000112516
Regions
	Domain	16 – 96	81	OPR
	Domain	133 – 150	18	Pseudo-CRIB
	Domain	157 – 250	94	PDZ
	Region	126 – 253	128	Interaction with PARD3 and CDC42 By similarity
Amino acid modifications
	Modified residue	11	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q9BYG5 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 79B5392D72B15BDC	FASTA	372	41,182
	10 20 30 40 50 60 MNRSHRHGAG SGCLGTMEVK SKFGAEFRRF SLERSKPGKF EEFYGLLQHV HKIPNVDVLV 70 80 90 100 110 120 GYADIHGDLL PINNDDNYHK AVSTANPLLR IFIQKKEEAD YSAFGTDTLI KKKNVLTNVL 130 140 150 160 170 180 RPDNHRKKPH IVISMPQDFR PVSSIIDVDI LPETHRRVRL YKYGTEKPLG FYIRDGSSVR 190 200 210 220 230 240 VTPHGLEKVP GIFISRLVPG GLAQSTGLLA VNDEVLEVNG IEVSGKSLDQ VTDMMIANSR 250 260 270 280 290 300 NLIITVRPAN QRNNVVRNSR TSGSSGQSTD NSLLGYPQQI EPSFEPEDED SEEDDIIIED 310 320 330 340 350 360 NGVPQQIPKA VPNTESLESL TQIELSFESG QNGFIPSNEV SLAAIASSSN TEFETHAPDQ 370 KLLEEDGTII TL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human homologues of the Caenorhabditis elegans cell polarity protein PAR6 as an adaptor that links the small GTPases Rac and Cdc42 to atypical protein kinase C." Noda Y., Takeya R., Ohno S., Naito S., Ito T., Sumimoto H. Genes Cells 6:107-119(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH PRKCI; PRKCZ; RAC1 AND CDC42. Tissue: Neuroblastoma.
[2]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[4]	"Multiple splice variants of Par3 and of a novel related gene, Par3L, produce proteins with different binding properties." Gao L., Macara I.G., Joberty G. Gene 294:99-107(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ALS2CR19 AND PARD3.
[5]	"Mammalian Lgl forms a protein complex with PAR-6 and aPKC independently of PAR-3 to regulate epithelial cell polarity." Yamanaka T., Horikoshi Y., Sugiyama Y., Ishiyama C., Suzuki A., Hirose T., Iwamatsu A., Shinohara A., Ohno S. Curr. Biol. 13:734-743(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LLGL1 AND PRKCI.
[6]	"Nucleotide exchange factor ECT2 interacts with the polarity protein complex Par6/Par3/protein kinase Czeta (PKCzeta) and regulates PKCzeta activity." Liu X.F., Ishida H., Raziuddin R., Miki T. Mol. Cell. Biol. 24:6665-6675(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ECT2.
[7]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB044555 mRNA. Translation: BAB40756.1. AL031680 Genomic DNA. Translation: CAB44747.3. BC060847 mRNA. Translation: AAH60847.1.
IPI	IPI00019025.
RefSeq	NP_115910.1. NM_032521.2.
UniGene	Hs.589848.
3D structure databases
ProteinModelPortal	Q9BYG5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BYG5. 12 interactions.
STRING	9606.ENSP00000360672.
PTM databases
PhosphoSite	Q9BYG5.
Polymorphism databases
DMDM	30913176.
Proteomic databases
PaxDb	Q9BYG5.
PRIDE	Q9BYG5.
Protocols and materials databases
DNASU	84612.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000371610; ENSP00000360672; ENSG00000124171.
GeneID	84612.
KEGG	hsa:84612.
UCSC	uc002xvo.3. human.
Organism-specific databases
CTD	84612.
GeneCards	GC20P049348.
HGNC	HGNC:16245. PARD6B.
HPA	HPA013376.
MIM	608975. gene.
neXtProt	NX_Q9BYG5.
PharmGKB	PA32938.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG315424.
HOGENOM	HOG000231716.
HOVERGEN	HBG053509.
InParanoid	Q9BYG5.
KO	K06093.
OMA	KKKPHIV.
OrthoDB	EOG4640CN.
PhylomeDB	Q9BYG5.
Enzyme and pathway databases
Reactome	REACT_111155. Cell-Cell communication.
Gene expression databases
ArrayExpress	Q9BYG5.
Bgee	Q9BYG5.
CleanEx	HS_PARD6B.
Genevestigator	Q9BYG5.
GermOnline	ENSG00000124171. Homo sapiens.
Family and domain databases
InterPro	IPR000270. OPR_PB1. IPR001478. PDZ. [Graphical view]
Pfam	PF00564. PB1. 1 hit. PF00595. PDZ. 1 hit. [Graphical view]
SMART	SM00666. PB1. 1 hit. SM00228. PDZ. 1 hit. [Graphical view]
SUPFAM	SSF50156. PDZ. 1 hit.
PROSITE	PS50106. PDZ. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	84612.
NextBio	74474.
SOURCE	Search...

Entry information

Entry name

PAR6B_HUMAN

Accession

Primary (citable) accession number: Q9BYG5
Secondary accession number(s): Q9Y510

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.