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Q9BYG5 (PAR6B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Partitioning defective 6 homolog beta

Short name=PAR-6 beta
Short name=PAR-6B
Gene names
Name:PARD6B
Synonyms:PAR6B
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.

Subunit structure

Interacts with PARD3. Interacts with GTP-bound forms of CDC42 and RAC1. Interacts with GTP-bound ARHQ/TC10. Interacts with MPP5 By similarity. Interacts with the N-terminal part of PRKCI and PRKCZ. Part of a complex with PARD3, CDC42 or RAC1 and PRKCI or PRKCZ. Part of a complex with LLGL1 and PRKCI. Interacts with ALS2CR19. Interacts with ECT2. Ref.1 Ref.5 Ref.6 Ref.7

Subcellular location

Cytoplasm. Cell membrane By similarity. Cell junctiontight junction By similarity.

Tissue specificity

Expressed in pancreas and in both adult and fetal kidney. Weakly expressed in placenta and lung. Not expressed in other tissues.

Domain

The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.

The PDZ domain mediates interaction with MPP5 By similarity.

Sequence similarities

Belongs to the PAR6 family.

Contains 1 OPR domain.

Contains 1 PDZ (DHR) domain.

Contains 1 pseudo-CRIB domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
   Cellular componentCell junction
Cell membrane
Cytoplasm
Membrane
Tight junction
   Coding sequence diversityAlternative splicing
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaxonogenesis

Traceable author statement PubMed 14676191. Source: UniProtKB

cell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

cell division

Inferred from electronic annotation. Source: UniProtKB-KW

cell junction assembly

Traceable author statement. Source: Reactome

cell-cell junction assembly

Traceable author statement PubMed 12545177. Source: UniProtKB

cell-cell junction organization

Traceable author statement. Source: Reactome

establishment or maintenance of cell polarity

Traceable author statement PubMed 14676191. Source: UniProtKB

protein complex assembly

Inferred from direct assay PubMed 12545177. Source: UniProtKB

regulation of cell migration

Traceable author statement PubMed 14676191. Source: UniProtKB

tight junction assembly

Traceable author statement. Source: Reactome

   Cellular_componentapical part of cell

Inferred from electronic annotation. Source: Ensembl

cell cortex

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from direct assay. Source: HPA

cytosol

Traceable author statement. Source: Reactome

extracellular vesicular exosome

Inferred from direct assay PubMed 19056867. Source: UniProt

nucleus

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from direct assay PubMed 12545177. Source: UniProtKB

protein complex

Inferred from electronic annotation. Source: Ensembl

tight junction

Inferred from direct assay PubMed 12545177. Source: UniProtKB

   Molecular_functionprotein binding

Inferred from physical interaction Ref.6PubMed 14676191Ref.7. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9BYG5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9BYG5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     97-113: EEADYSAFGTDTLIKKK → GCVLEHSKNNVRMPCLK
     114-370: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Partitioning defective 6 homolog beta
PRO_0000112516

Regions

Domain16 – 9681OPR
Domain133 – 15018Pseudo-CRIB
Domain157 – 25094PDZ
Region126 – 253128Interaction with PARD3 and CDC42 By similarity

Amino acid modifications

Modified residue111Phosphoserine Ref.8

Natural variations

Alternative sequence97 – 11317EEADY…LIKKK → GCVLEHSKNNVRMPCLK in isoform 2.
VSP_053311
Alternative sequence114 – 370257Missing in isoform 2.
VSP_053312

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 79B5392D72B15BDC

FASTA37241,182
        10         20         30         40         50         60 
MNRSHRHGAG SGCLGTMEVK SKFGAEFRRF SLERSKPGKF EEFYGLLQHV HKIPNVDVLV 

        70         80         90        100        110        120 
GYADIHGDLL PINNDDNYHK AVSTANPLLR IFIQKKEEAD YSAFGTDTLI KKKNVLTNVL 

       130        140        150        160        170        180 
RPDNHRKKPH IVISMPQDFR PVSSIIDVDI LPETHRRVRL YKYGTEKPLG FYIRDGSSVR 

       190        200        210        220        230        240 
VTPHGLEKVP GIFISRLVPG GLAQSTGLLA VNDEVLEVNG IEVSGKSLDQ VTDMMIANSR 

       250        260        270        280        290        300 
NLIITVRPAN QRNNVVRNSR TSGSSGQSTD NSLLGYPQQI EPSFEPEDED SEEDDIIIED 

       310        320        330        340        350        360 
NGVPQQIPKA VPNTESLESL TQIELSFESG QNGFIPSNEV SLAAIASSSN TEFETHAPDQ 

       370 
KLLEEDGTII TL 

« Hide

Isoform 2 [UniParc].

Checksum: 65666757F08F7157
Show »

FASTA11512,989

References

« Hide 'large scale' references
[1]"Human homologues of the Caenorhabditis elegans cell polarity protein PAR6 as an adaptor that links the small GTPases Rac and Cdc42 to atypical protein kinase C."
Noda Y., Takeya R., Ohno S., Naito S., Ito T., Sumimoto H.
Genes Cells 6:107-119(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH PRKCI; PRKCZ; RAC1 AND CDC42.
Tissue: Neuroblastoma.
[2]"Splicing variants of the cell polarity protein PAR-6."
Noda Y., Kohjima M., Izaki T., Sumimoto H.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Placenta.
[5]"Multiple splice variants of Par3 and of a novel related gene, Par3L, produce proteins with different binding properties."
Gao L., Macara I.G., Joberty G.
Gene 294:99-107(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ALS2CR19 AND PARD3.
[6]"Mammalian Lgl forms a protein complex with PAR-6 and aPKC independently of PAR-3 to regulate epithelial cell polarity."
Yamanaka T., Horikoshi Y., Sugiyama Y., Ishiyama C., Suzuki A., Hirose T., Iwamatsu A., Shinohara A., Ohno S.
Curr. Biol. 13:734-743(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LLGL1 AND PRKCI.
[7]"Nucleotide exchange factor ECT2 interacts with the polarity protein complex Par6/Par3/protein kinase Czeta (PKCzeta) and regulates PKCzeta activity."
Liu X.F., Ishida H., Raziuddin R., Miki T.
Mol. Cell. Biol. 24:6665-6675(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ECT2.
[8]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB044555 mRNA. Translation: BAB40756.1.
AB178534 mRNA. Translation: BAF92013.1.
AL031680 Genomic DNA. Translation: CAB44747.3.
BC060847 mRNA. Translation: AAH60847.1.
CCDSCCDS33485.1. [Q9BYG5-1]
RefSeqNP_115910.1. NM_032521.2. [Q9BYG5-1]
UniGeneHs.589848.

3D structure databases

ProteinModelPortalQ9BYG5.
SMRQ9BYG5. Positions 17-96, 131-253.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid124145. 16 interactions.
IntActQ9BYG5. 12 interactions.
MINTMINT-5001972.
STRING9606.ENSP00000360672.

PTM databases

PhosphoSiteQ9BYG5.

Polymorphism databases

DMDM30913176.

Proteomic databases

MaxQBQ9BYG5.
PaxDbQ9BYG5.
PRIDEQ9BYG5.

Protocols and materials databases

DNASU84612.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000371610; ENSP00000360672; ENSG00000124171. [Q9BYG5-1]
ENST00000396039; ENSP00000379354; ENSG00000124171. [Q9BYG5-2]
GeneID84612.
KEGGhsa:84612.
UCSCuc002xvo.3. human. [Q9BYG5-1]

Organism-specific databases

CTD84612.
GeneCardsGC20P049348.
HGNCHGNC:16245. PARD6B.
HPAHPA013376.
MIM608975. gene.
neXtProtNX_Q9BYG5.
PharmGKBPA32938.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG315424.
HOGENOMHOG000231716.
HOVERGENHBG053509.
InParanoidQ9BYG5.
KOK06093.
OMAEPQQIPK.
OrthoDBEOG73JKWB.
PhylomeDBQ9BYG5.
TreeFamTF312899.

Enzyme and pathway databases

ReactomeREACT_111155. Cell-Cell communication.

Gene expression databases

ArrayExpressQ9BYG5.
BgeeQ9BYG5.
CleanExHS_PARD6B.
GenevestigatorQ9BYG5.

Family and domain databases

Gene3D2.30.42.10. 1 hit.
InterProIPR000270. OPR_PB1.
IPR001478. PDZ.
[Graphical view]
PfamPF00564. PB1. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTSM00666. PB1. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMSSF50156. SSF50156. 1 hit.
PROSITEPS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiPARD6B.
GenomeRNAi84612.
NextBio35460691.
PROQ9BYG5.
SOURCESearch...

Entry information

Entry namePAR6B_HUMAN
AccessionPrimary (citable) accession number: Q9BYG5
Secondary accession number(s): A2A2A7, Q9Y510
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM