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Protein

Partitioning defective 6 homolog beta

Gene

PARD6B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.

GO - Biological processi

  1. axonogenesis Source: UniProtKB
  2. cell-cell junction assembly Source: UniProtKB
  3. cell-cell junction organization Source: Reactome
  4. cell cycle Source: UniProtKB-KW
  5. cell division Source: UniProtKB-KW
  6. cell junction assembly Source: Reactome
  7. establishment or maintenance of cell polarity Source: UniProtKB
  8. protein complex assembly Source: UniProtKB
  9. regulation of cell migration Source: UniProtKB
  10. tight junction assembly Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

ReactomeiREACT_19373. Tight junction interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Partitioning defective 6 homolog beta
Short name:
PAR-6 beta
Short name:
PAR-6B
Gene namesi
Name:PARD6B
Synonyms:PAR6B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:16245. PARD6B.

Subcellular locationi

Cytoplasm. Cell membrane By similarity. Cell junctiontight junction By similarity

GO - Cellular componenti

  1. apical part of cell Source: Ensembl
  2. cell cortex Source: Ensembl
  3. cytoplasm Source: HPA
  4. cytosol Source: Reactome
  5. extracellular vesicular exosome Source: UniProtKB
  6. nucleus Source: Ensembl
  7. plasma membrane Source: UniProtKB
  8. protein complex Source: Ensembl
  9. tight junction Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Tight junction

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA32938.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Partitioning defective 6 homolog betaPRO_0000112516Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9BYG5.
PaxDbiQ9BYG5.
PRIDEiQ9BYG5.

PTM databases

PhosphoSiteiQ9BYG5.

Expressioni

Tissue specificityi

Expressed in pancreas and in both adult and fetal kidney. Weakly expressed in placenta and lung. Not expressed in other tissues.

Gene expression databases

BgeeiQ9BYG5.
CleanExiHS_PARD6B.
GenevestigatoriQ9BYG5.

Organism-specific databases

HPAiHPA013376.

Interactioni

Subunit structurei

Interacts with PARD3. Interacts with GTP-bound forms of CDC42 and RAC1. Interacts with GTP-bound ARHQ/TC10. Interacts with MPP5 (By similarity). Interacts with the N-terminal part of PRKCI and PRKCZ. Part of a complex with PARD3, CDC42 or RAC1 and PRKCI or PRKCZ. Part of a complex with LLGL1 and PRKCI. Interacts with ALS2CR19. Interacts with ECT2.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC42P609537EBI-295391,EBI-81752
PARD3Q8TEW04EBI-295391,EBI-81968
PNMA1Q8ND902EBI-295391,EBI-302345
PRKCIP417439EBI-295391,EBI-286199
PRKCZQ055133EBI-295391,EBI-295351
RAC1P630003EBI-295391,EBI-413628
YWHAHQ049172EBI-295391,EBI-306940

Protein-protein interaction databases

BioGridi124145. 31 interactions.
IntActiQ9BYG5. 12 interactions.
MINTiMINT-5001972.
STRINGi9606.ENSP00000360672.

Structurei

3D structure databases

ProteinModelPortaliQ9BYG5.
SMRiQ9BYG5. Positions 17-96, 131-253.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 9681OPRAdd
BLAST
Domaini133 – 15018Pseudo-CRIBAdd
BLAST
Domaini157 – 25094PDZPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 253128Interaction with PARD3 and CDC42By similarityAdd
BLAST

Domaini

The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
The PDZ domain mediates interaction with MPP5.By similarity

Sequence similaritiesi

Belongs to the PAR6 family.Curated
Contains 1 OPR domain.Curated
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 pseudo-CRIB domain.Curated

Phylogenomic databases

eggNOGiNOG315424.
GeneTreeiENSGT00390000005255.
HOGENOMiHOG000231716.
HOVERGENiHBG053509.
InParanoidiQ9BYG5.
KOiK06093.
OMAiEPQQIPK.
OrthoDBiEOG73JKWB.
PhylomeDBiQ9BYG5.
TreeFamiTF312899.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR000270. OPR_PB1.
IPR001478. PDZ.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9BYG5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRSHRHGAG SGCLGTMEVK SKFGAEFRRF SLERSKPGKF EEFYGLLQHV
60 70 80 90 100
HKIPNVDVLV GYADIHGDLL PINNDDNYHK AVSTANPLLR IFIQKKEEAD
110 120 130 140 150
YSAFGTDTLI KKKNVLTNVL RPDNHRKKPH IVISMPQDFR PVSSIIDVDI
160 170 180 190 200
LPETHRRVRL YKYGTEKPLG FYIRDGSSVR VTPHGLEKVP GIFISRLVPG
210 220 230 240 250
GLAQSTGLLA VNDEVLEVNG IEVSGKSLDQ VTDMMIANSR NLIITVRPAN
260 270 280 290 300
QRNNVVRNSR TSGSSGQSTD NSLLGYPQQI EPSFEPEDED SEEDDIIIED
310 320 330 340 350
NGVPQQIPKA VPNTESLESL TQIELSFESG QNGFIPSNEV SLAAIASSSN
360 370
TEFETHAPDQ KLLEEDGTII TL
Length:372
Mass (Da):41,182
Last modified:June 1, 2001 - v1
Checksum:i79B5392D72B15BDC
GO
Isoform 2 (identifier: Q9BYG5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     97-113: EEADYSAFGTDTLIKKK → GCVLEHSKNNVRMPCLK
     114-370: Missing.

Show »
Length:115
Mass (Da):12,989
Checksum:i65666757F08F7157
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei97 – 11317EEADY…LIKKK → GCVLEHSKNNVRMPCLK in isoform 2. 1 PublicationVSP_053311Add
BLAST
Alternative sequencei114 – 370257Missing in isoform 2. 1 PublicationVSP_053312Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB044555 mRNA. Translation: BAB40756.1.
AB178534 mRNA. Translation: BAF92013.1.
AL031680 Genomic DNA. Translation: CAB44747.3.
BC060847 mRNA. Translation: AAH60847.1.
CCDSiCCDS33485.1. [Q9BYG5-1]
RefSeqiNP_115910.1. NM_032521.2. [Q9BYG5-1]
UniGeneiHs.589848.

Genome annotation databases

EnsembliENST00000371610; ENSP00000360672; ENSG00000124171. [Q9BYG5-1]
ENST00000396039; ENSP00000379354; ENSG00000124171. [Q9BYG5-2]
GeneIDi84612.
KEGGihsa:84612.
UCSCiuc002xvo.3. human. [Q9BYG5-1]

Polymorphism databases

DMDMi30913176.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB044555 mRNA. Translation: BAB40756.1.
AB178534 mRNA. Translation: BAF92013.1.
AL031680 Genomic DNA. Translation: CAB44747.3.
BC060847 mRNA. Translation: AAH60847.1.
CCDSiCCDS33485.1. [Q9BYG5-1]
RefSeqiNP_115910.1. NM_032521.2. [Q9BYG5-1]
UniGeneiHs.589848.

3D structure databases

ProteinModelPortaliQ9BYG5.
SMRiQ9BYG5. Positions 17-96, 131-253.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124145. 31 interactions.
IntActiQ9BYG5. 12 interactions.
MINTiMINT-5001972.
STRINGi9606.ENSP00000360672.

PTM databases

PhosphoSiteiQ9BYG5.

Polymorphism databases

DMDMi30913176.

Proteomic databases

MaxQBiQ9BYG5.
PaxDbiQ9BYG5.
PRIDEiQ9BYG5.

Protocols and materials databases

DNASUi84612.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371610; ENSP00000360672; ENSG00000124171. [Q9BYG5-1]
ENST00000396039; ENSP00000379354; ENSG00000124171. [Q9BYG5-2]
GeneIDi84612.
KEGGihsa:84612.
UCSCiuc002xvo.3. human. [Q9BYG5-1]

Organism-specific databases

CTDi84612.
GeneCardsiGC20P049348.
HGNCiHGNC:16245. PARD6B.
HPAiHPA013376.
MIMi608975. gene.
neXtProtiNX_Q9BYG5.
PharmGKBiPA32938.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG315424.
GeneTreeiENSGT00390000005255.
HOGENOMiHOG000231716.
HOVERGENiHBG053509.
InParanoidiQ9BYG5.
KOiK06093.
OMAiEPQQIPK.
OrthoDBiEOG73JKWB.
PhylomeDBiQ9BYG5.
TreeFamiTF312899.

Enzyme and pathway databases

ReactomeiREACT_19373. Tight junction interactions.

Miscellaneous databases

GeneWikiiPARD6B.
GenomeRNAii84612.
NextBioi35460691.
PROiQ9BYG5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BYG5.
CleanExiHS_PARD6B.
GenevestigatoriQ9BYG5.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR000270. OPR_PB1.
IPR001478. PDZ.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human homologues of the Caenorhabditis elegans cell polarity protein PAR6 as an adaptor that links the small GTPases Rac and Cdc42 to atypical protein kinase C."
    Noda Y., Takeya R., Ohno S., Naito S., Ito T., Sumimoto H.
    Genes Cells 6:107-119(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH PRKCI; PRKCZ; RAC1 AND CDC42.
    Tissue: Neuroblastoma.
  2. "Splicing variants of the cell polarity protein PAR-6."
    Noda Y., Kohjima M., Izaki T., Sumimoto H.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  3. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  5. "Multiple splice variants of Par3 and of a novel related gene, Par3L, produce proteins with different binding properties."
    Gao L., Macara I.G., Joberty G.
    Gene 294:99-107(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ALS2CR19 AND PARD3.
  6. "Mammalian Lgl forms a protein complex with PAR-6 and aPKC independently of PAR-3 to regulate epithelial cell polarity."
    Yamanaka T., Horikoshi Y., Sugiyama Y., Ishiyama C., Suzuki A., Hirose T., Iwamatsu A., Shinohara A., Ohno S.
    Curr. Biol. 13:734-743(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LLGL1 AND PRKCI.
  7. "Nucleotide exchange factor ECT2 interacts with the polarity protein complex Par6/Par3/protein kinase Czeta (PKCzeta) and regulates PKCzeta activity."
    Liu X.F., Ishida H., Raziuddin R., Miki T.
    Mol. Cell. Biol. 24:6665-6675(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ECT2.
  8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPAR6B_HUMAN
AccessioniPrimary (citable) accession number: Q9BYG5
Secondary accession number(s): A2A2A7, Q9Y510
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: June 1, 2001
Last modified: February 4, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.