ID   PAR6G_HUMAN             Reviewed;         376 AA.
AC   Q9BYG4; A8QM57;
DT   16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   24-JAN-2024, entry version 165.
DE   RecName: Full=Partitioning defective 6 homolog gamma;
DE            Short=PAR-6 gamma;
DE   AltName: Full=PAR6D;
GN   Name=PARD6G; Synonyms=PAR6G;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND INTERACTION WITH RAC1; CDC42;
RP   PRKCI AND PRKCZ.
RC   TISSUE=Neuroblastoma;
RX   PubMed=11260256; DOI=10.1046/j.1365-2443.2001.00404.x;
RA   Noda Y., Takeya R., Ohno S., Naito S., Ito T., Sumimoto H.;
RT   "Human homologues of the Caenorhabditis elegans cell polarity protein PAR6
RT   as an adaptor that links the small GTPases Rac and Cdc42 to atypical
RT   protein kinase C.";
RL   Genes Cells 6:107-119(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA   Noda Y., Kohjima M., Izaki T., Sumimoto H.;
RT   "Splicing variants of the cell polarity protein PAR-6.";
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16177791; DOI=10.1038/nature03983;
RA   Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D.,
RA   Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X.,
RA   Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J.,
RA   Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L.,
RA   Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A.,
RA   Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C.,
RA   Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K.,
RA   Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R.,
RA   Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.;
RT   "DNA sequence and analysis of human chromosome 18.";
RL   Nature 437:551-555(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Placenta;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   INTERACTION WITH RHOQ.
RX   PubMed=10934474; DOI=10.1038/35019573;
RA   Joberty G., Petersen C., Gao L., Macara I.G.;
RT   "The cell-polarity protein Par6 links Par3 and atypical protein kinase C to
RT   Cdc42.";
RL   Nat. Cell Biol. 2:531-539(2000).
CC   -!- FUNCTION: Adapter protein involved in asymmetrical cell division and
CC       cell polarization processes. May play a role in the formation of
CC       epithelial tight junctions. The PARD6-PARD3 complex links GTP-bound Rho
CC       small GTPases to atypical protein kinase C proteins (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with PARD3 (Probable). Interacts with GTP-bound
CC       forms of CDC42, RHOQ/TC10 and RAC1. Interacts with the N-terminal part
CC       of PRKCI and PRKCZ. {ECO:0000269|PubMed:10934474,
CC       ECO:0000269|PubMed:11260256, ECO:0000305}.
CC   -!- INTERACTION:
CC       Q9BYG4; P60953: CDC42; NbExp=5; IntAct=EBI-295417, EBI-81752;
CC       Q9BYG4; Q7KZI7: MARK2; NbExp=2; IntAct=EBI-295417, EBI-516560;
CC       Q9BYG4; Q96L34: MARK4; NbExp=2; IntAct=EBI-295417, EBI-302319;
CC       Q9BYG4; Q8TEW0: PARD3; NbExp=3; IntAct=EBI-295417, EBI-81968;
CC       Q9BYG4; P41743: PRKCI; NbExp=7; IntAct=EBI-295417, EBI-286199;
CC       Q9BYG4; Q05513: PRKCZ; NbExp=7; IntAct=EBI-295417, EBI-295351;
CC       Q9BYG4; P63000: RAC1; NbExp=2; IntAct=EBI-295417, EBI-413628;
CC       Q9BYG4; Q04917: YWHAH; NbExp=2; IntAct=EBI-295417, EBI-306940;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Cell membrane {ECO:0000250}. Cell
CC       junction, tight junction {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9BYG4-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9BYG4-2; Sequence=VSP_047586, VSP_047587;
CC   -!- TISSUE SPECIFICITY: Widely expressed, with a higher expression in fetal
CC       and adult kidney.
CC   -!- DOMAIN: The pseudo-CRIB domain together with the PDZ domain is required
CC       for the interaction with Rho small GTPases. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PAR6 family. {ECO:0000305}.
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DR   EMBL; AB044556; BAB40757.1; -; mRNA.
DR   EMBL; AB178536; BAF92015.1; -; mRNA.
DR   EMBL; AC139100; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC060797; AAH60797.1; -; mRNA.
DR   CCDS; CCDS12022.1; -. [Q9BYG4-1]
DR   RefSeq; NP_115899.1; NM_032510.3. [Q9BYG4-1]
DR   AlphaFoldDB; Q9BYG4; -.
DR   SMR; Q9BYG4; -.
DR   BioGRID; 124135; 42.
DR   ComplexPortal; CPX-6194; PAR cell polarity complex, PARD6G-PRKCI variant.
DR   ComplexPortal; CPX-6195; PAR cell polarity complex, PARD6G-PRKCZ variant.
DR   IntAct; Q9BYG4; 16.
DR   MINT; Q9BYG4; -.
DR   STRING; 9606.ENSP00000343144; -.
DR   iPTMnet; Q9BYG4; -.
DR   PhosphoSitePlus; Q9BYG4; -.
DR   BioMuta; PARD6G; -.
DR   DMDM; 30913175; -.
DR   EPD; Q9BYG4; -.
DR   jPOST; Q9BYG4; -.
DR   MassIVE; Q9BYG4; -.
DR   MaxQB; Q9BYG4; -.
DR   PaxDb; 9606-ENSP00000343144; -.
DR   PeptideAtlas; Q9BYG4; -.
DR   ProteomicsDB; 79638; -. [Q9BYG4-1]
DR   Pumba; Q9BYG4; -.
DR   Antibodypedia; 23537; 121 antibodies from 19 providers.
DR   DNASU; 84552; -.
DR   Ensembl; ENST00000353265.8; ENSP00000343144.3; ENSG00000178184.16. [Q9BYG4-1]
DR   Ensembl; ENST00000470488.2; ENSP00000468735.1; ENSG00000178184.16. [Q9BYG4-2]
DR   GeneID; 84552; -.
DR   KEGG; hsa:84552; -.
DR   MANE-Select; ENST00000353265.8; ENSP00000343144.3; NM_032510.4; NP_115899.1.
DR   UCSC; uc002lny.4; human. [Q9BYG4-1]
DR   AGR; HGNC:16076; -.
DR   CTD; 84552; -.
DR   DisGeNET; 84552; -.
DR   GeneCards; PARD6G; -.
DR   HGNC; HGNC:16076; PARD6G.
DR   HPA; ENSG00000178184; Tissue enhanced (skin).
DR   MIM; 608976; gene.
DR   neXtProt; NX_Q9BYG4; -.
DR   OpenTargets; ENSG00000178184; -.
DR   PharmGKB; PA32939; -.
DR   VEuPathDB; HostDB:ENSG00000178184; -.
DR   eggNOG; KOG3606; Eukaryota.
DR   GeneTree; ENSGT00950000183211; -.
DR   HOGENOM; CLU_040653_2_0_1; -.
DR   InParanoid; Q9BYG4; -.
DR   OMA; TEQSFDK; -.
DR   OrthoDB; 5401122at2759; -.
DR   PhylomeDB; Q9BYG4; -.
DR   TreeFam; TF312899; -.
DR   PathwayCommons; Q9BYG4; -.
DR   Reactome; R-HSA-420029; Tight junction interactions.
DR   SignaLink; Q9BYG4; -.
DR   BioGRID-ORCS; 84552; 9 hits in 1152 CRISPR screens.
DR   ChiTaRS; PARD6G; human.
DR   GenomeRNAi; 84552; -.
DR   Pharos; Q9BYG4; Tbio.
DR   PRO; PR:Q9BYG4; -.
DR   Proteomes; UP000005640; Chromosome 18.
DR   RNAct; Q9BYG4; Protein.
DR   Bgee; ENSG00000178184; Expressed in upper arm skin and 159 other cell types or tissues.
DR   ExpressionAtlas; Q9BYG4; baseline and differential.
DR   GO; GO:0016324; C:apical plasma membrane; IBA:GO_Central.
DR   GO; GO:0005923; C:bicellular tight junction; IEA:UniProtKB-SubCell.
DR   GO; GO:0005938; C:cell cortex; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0120157; C:PAR polarity complex; ISS:ComplexPortal.
DR   GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR   GO; GO:0070160; C:tight junction; NAS:ComplexPortal.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007098; P:centrosome cycle; IBA:GO_Central.
DR   GO; GO:0007163; P:establishment or maintenance of cell polarity; IBA:GO_Central.
DR   GO; GO:0045197; P:establishment or maintenance of epithelial cell apical/basal polarity; ISS:ComplexPortal.
DR   GO; GO:0060341; P:regulation of cellular localization; IBA:GO_Central.
DR   CDD; cd06403; PB1_Par6; 1.
DR   CDD; cd00992; PDZ_signaling; 1.
DR   Gene3D; 2.30.42.10; -; 1.
DR   InterPro; IPR000270; PB1_dom.
DR   InterPro; IPR034868; PB1_Par6.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR036034; PDZ_sf.
DR   PANTHER; PTHR14102; PAR-6-RELATED; 1.
DR   PANTHER; PTHR14102:SF3; PARTITIONING DEFECTIVE 6 HOMOLOG GAMMA; 1.
DR   Pfam; PF00564; PB1; 1.
DR   Pfam; PF00595; PDZ; 1.
DR   SMART; SM00666; PB1; 1.
DR   SMART; SM00228; PDZ; 1.
DR   SUPFAM; SSF54277; CAD & PB1 domains; 1.
DR   SUPFAM; SSF50156; PDZ domain-like; 1.
DR   PROSITE; PS51745; PB1; 1.
DR   PROSITE; PS50106; PDZ; 1.
DR   Genevisible; Q9BYG4; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cell cycle; Cell division; Cell junction;
KW   Cell membrane; Cytoplasm; Membrane; Reference proteome; Tight junction.
FT   CHAIN           1..376
FT                   /note="Partitioning defective 6 homolog gamma"
FT                   /id="PRO_0000112518"
FT   DOMAIN          18..98
FT                   /note="PB1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01081"
FT   DOMAIN          134..151
FT                   /note="Pseudo-CRIB"
FT   DOMAIN          158..251
FT                   /note="PDZ"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00143"
FT   REGION          127..254
FT                   /note="Interaction with PARD3 and CDC42"
FT                   /evidence="ECO:0000250"
FT   REGION          356..376
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         99..107
FT                   /note="EEAERGSLG -> DDGALRPGT (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.2"
FT                   /id="VSP_047586"
FT   VAR_SEQ         108..376
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.2"
FT                   /id="VSP_047587"
SQ   SEQUENCE   376 AA;  40883 MW;  E5A4EF521403CA11 CRC64;
     MNRSFHKSQT LRFYDCSAVE VKSKFGAEFR RFSLDRHKPG KFEDFYKLVV HTHHISNSDV
     TIGYADVHGD LLPINNDDNF CKAVSSANPL LRVFIQKREE AERGSLGAGS LCRRRRALGA
     LRDEGPRRRA HLDIGLPRDF RPVSSIIDVD LVPETHRRVR LHRHGCEKPL GFYIRDGASV
     RVTPHGLEKV PGIFISRMVP GGLAESTGLL AVNDEVLEVN GIEVAGKTLD QVTDMMIANS
     HNLIVTVKPA NQRNNVVRGG RALGSSGPPS DGTAGFVGPP APRVLQNFHP DEAESDEDND
     VVIEGTLEPA RPPQTPGAPA GSLSRVNGAG LAQRLQRDLA LDGGLQRLLS SLRADPRHSL
     ALPPGGVEEH GPAVTL
//