Q9BYG0 (B3GN5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
Short name=BGnT-5
Short name=Beta-1,3-Gn-T5
Short name=Beta-1,3-N-acetylglucosaminyltransferase 5
Short name=Beta3Gn-T5
EC=2.4.1.-

Alternative name(s):
Lactotriaosylceramide synthase
Short name=Lc(3)Cer synthase
Short name=Lc3 synthase

Gene names

Name:

B3GNT5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc4Cer; paragloboside), resulting in the synthesis of Lc3Cer and neolactopentaosylceramide (nLc5Cer), respectively. Probably plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Widely expressed. Highly expressed in lung, colon, placenta, testis, pituitary gland and cerebellum. Weakly expressed in brain, liver, spleen, lymph node and thymus. Ref.1
Induction	Up-regulated by stimulation with retinoic acid and down-regulated with 12-O-tetradecanoylphorbol-13-acetate (TPA). Ref.1
Sequence similarities	Belongs to the glycosyltransferase 31 family.
Sequence caution	The sequence BAD92754.1 differs from that shown. Reason: Frameshift at position 347.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Developmental protein Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	central nervous system development Inferred from direct assay Ref.2. Source: UniProtKB glycolipid biosynthetic process Traceable author statement Ref.2. Source: UniProtKB protein glycosylation Traceable author statement Ref.2. Source: UniProtKB
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide beta-1,3-acetylglucosaminyltransferase activity Inferred from direct assay Ref.2. Source: UniProtKB galactosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5		PRO_0000289209
Regions
	Topological domain	1 – 14	14	Cytoplasmic Potential
	Transmembrane	15 – 35	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	36 – 378	343	Lumenal Potential
Amino acid modifications
	Glycosylation	59	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9BYG0 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: E19BA966A5E12CD2	FASTA	378	44,053
	10 20 30 40 50 60 MRMLVSGRRV KKWQLIIQLF ATCFLASLMF FWEPIDNHIV SHMKSYSYRY LINSYDFVND 70 80 90 100 110 120 TLSLKHTSAG PRYQYLINHK EKCQAQDVLL LLFVKTAPEN YDRRSGIRRT WGNENYVRSQ 130 140 150 160 170 180 LNANIKTLFA LGTPNPLEGE ELQRKLAWED QRYNDIIQQD FVDSFYNLTL KLLMQFSWAN 190 200 210 220 230 240 TYCPHAKFLM TADDDIFIHM PNLIEYLQSL EQIGVQDFWI GRVHRGAPPI RDKSSKYYVS 250 260 270 280 290 300 YEMYQWPAYP DYTAGAAYVI SGDVAAKVYE ASQTLNSSLY IDDVFMGLCA NKIGIVPQDH 310 320 330 340 350 360 VFFSGEGKTP YHPCIYEKMM TSHGHLEDLQ DLWKNATDPK VKTISKGFFG QIYCRLMKII 370 LLCKISYVDT YPCRAAFI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta 1,3-N-acetylglucosaminyltransferase (beta 3Gn-T5), an essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids." Togayachi A., Akashima T., Ookubo R., Kudo T., Nishihara S., Iwasaki H., Natsume A., Mio H., Inokuchi J., Irimura T., Sasaki K., Narimatsu H. J. Biol. Chem. 276:22032-22040(2001) [PubMed: 11283017] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, TISSUE SPECIFICITY, INDUCTION.
[2]	"Cloning of a mouse beta 1,3 N-acetylglucosaminyltransferase GlcNAc(beta 1,3)Gal(beta 1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis." Henion T.R., Zhou D., Wolfer D.P., Jungalwala F.B., Hennet T. J. Biol. Chem. 276:30261-30269(2001) [PubMed: 11384981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	Bennett E.P. Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Beta1,3-N-acetylglucosaminyltransferase 5

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB045278 mRNA. Translation: BAB40940.1. AF368169 mRNA. Translation: AAK53403.1. AJ304505 mRNA. Translation: CAC83093.1. AB209517 mRNA. Translation: BAD92754.1. Frameshift. CH471052 Genomic DNA. Translation: EAW78333.1. CH471052 Genomic DNA. Translation: EAW78334.1. CH471052 Genomic DNA. Translation: EAW78335.1. CH471052 Genomic DNA. Translation: EAW78336.1. BC028058 mRNA. Translation: AAH28058.1.
IPI	IPI00012508.
RefSeq	NP_114436.1. NM_032047.4.
UniGene	Hs.718506.
3D structure databases
ProteinModelPortal	Q9BYG0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9BYG0. 1 interaction.
STRING	Q9BYG0.
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Polymorphism databases
DMDM	74733473.
Proteomic databases
PRIDE	Q9BYG0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000326505; ENSP00000316173; ENSG00000176597. ENST00000460419; ENSP00000420778; ENSG00000176597. ENST00000465010; ENSP00000417868; ENSG00000176597.
GeneID	84002.
KEGG	hsa:84002.
UCSC	uc003flk.1. human.
Organism-specific databases
CTD	84002.
GeneCards	GC03P182971.
H-InvDB	HIX0003898.
HGNC	HGNC:15684. B3GNT5.
HPA	HPA017292.
neXtProt	NX_Q9BYG0.
PharmGKB	PA25221.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17451.
GeneTree	ENSGT00560000076821.
HOGENOM	HBG446090.
HOVERGEN	HBG101684.
InParanoid	Q9BYG0.
OMA	YTAGAAY.
OrthoDB	EOG4PNXH6.
PhylomeDB	Q9BYG0.
Enzyme and pathway databases
BRENDA	2.4.1.206. 2681.
Gene expression databases
ArrayExpress	Q9BYG0.
Bgee	Q9BYG0.
CleanEx	HS_B3GNT5.
Genevestigator	Q9BYG0.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
KO	K03766.
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	73170.

Entry information

Entry name

B3GN5_HUMAN

Accession

Primary (citable) accession number: Q9BYG0
Secondary accession number(s): D3DNS5 , Q59FE3, Q7L9Z5, Q8WWP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.