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Protein

Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase

Gene

B3GNT5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc4Cer; paragloboside), resulting in the synthesis of Lc3Cer and neolactopentaosylceramide (nLc5Cer), respectively. Probably plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • brain development Source: Ensembl
  • central nervous system development Source: UniProtKB
  • glycolipid biosynthetic process Source: UniProtKB
  • O-glycan processing Source: Reactome
  • protein glycosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.206. 2681.
ReactomeiR-HSA-913709. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Chemistry

SwissLipidsiSLP:000000768.

Names & Taxonomyi

Protein namesi
Recommended name:
Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase (EC:2.4.1.206)
Alternative name(s):
Lactotriaosylceramide synthase
Short name:
Lc(3)Cer synthase
Short name:
Lc3 synthase
UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5
Short name:
BGnT-5
Short name:
Beta-1,3-Gn-T5
Short name:
Beta-1,3-N-acetylglucosaminyltransferase 5
Short name:
Beta3Gn-T5
Gene namesi
Name:B3GNT5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:15684. B3GNT5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414CytoplasmicSequence analysisAdd
BLAST
Transmembranei15 – 3521Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini36 – 378343LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25221.

Polymorphism and mutation databases

BioMutaiB3GNT5.
DMDMi74733473.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 378378Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferasePRO_0000289209Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi59 – 591N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9BYG0.
PaxDbiQ9BYG0.
PeptideAtlasiQ9BYG0.
PRIDEiQ9BYG0.

PTM databases

PhosphoSiteiQ9BYG0.

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in lung, colon, placenta, testis, pituitary gland and cerebellum. Weakly expressed in brain, liver, spleen, lymph node and thymus.1 Publication

Inductioni

Up-regulated by stimulation with retinoic acid and down-regulated with 12-O-tetradecanoylphorbol-13-acetate (TPA).1 Publication

Gene expression databases

BgeeiENSG00000176597.
CleanExiHS_B3GNT5.
ExpressionAtlasiQ9BYG0. baseline and differential.
GenevisibleiQ9BYG0. HS.

Organism-specific databases

HPAiHPA017292.

Interactioni

Protein-protein interaction databases

BioGridi123846. 1 interaction.
IntActiQ9BYG0. 1 interaction.
STRINGi9606.ENSP00000316173.

Structurei

3D structure databases

ProteinModelPortaliQ9BYG0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000064519.
HOVERGENiHBG101684.
InParanoidiQ9BYG0.
KOiK03766.
OMAiTWGNEKY.
OrthoDBiEOG091G0AXM.
PhylomeDBiQ9BYG0.
TreeFamiTF318639.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9BYG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMLVSGRRV KKWQLIIQLF ATCFLASLMF FWEPIDNHIV SHMKSYSYRY
60 70 80 90 100
LINSYDFVND TLSLKHTSAG PRYQYLINHK EKCQAQDVLL LLFVKTAPEN
110 120 130 140 150
YDRRSGIRRT WGNENYVRSQ LNANIKTLFA LGTPNPLEGE ELQRKLAWED
160 170 180 190 200
QRYNDIIQQD FVDSFYNLTL KLLMQFSWAN TYCPHAKFLM TADDDIFIHM
210 220 230 240 250
PNLIEYLQSL EQIGVQDFWI GRVHRGAPPI RDKSSKYYVS YEMYQWPAYP
260 270 280 290 300
DYTAGAAYVI SGDVAAKVYE ASQTLNSSLY IDDVFMGLCA NKIGIVPQDH
310 320 330 340 350
VFFSGEGKTP YHPCIYEKMM TSHGHLEDLQ DLWKNATDPK VKTISKGFFG
360 370
QIYCRLMKII LLCKISYVDT YPCRAAFI
Length:378
Mass (Da):44,053
Last modified:June 1, 2001 - v1
Checksum:iE19BA966A5E12CD2
GO

Sequence cautioni

The sequence BAD92754 differs from that shown. Reason: Frameshift at position 347. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB045278 mRNA. Translation: BAB40940.1.
AF368169 mRNA. Translation: AAK53403.1.
AJ304505 mRNA. Translation: CAC83093.1.
AB209517 mRNA. Translation: BAD92754.1. Frameshift.
CH471052 Genomic DNA. Translation: EAW78333.1.
CH471052 Genomic DNA. Translation: EAW78334.1.
CH471052 Genomic DNA. Translation: EAW78335.1.
CH471052 Genomic DNA. Translation: EAW78336.1.
BC028058 mRNA. Translation: AAH28058.1.
CCDSiCCDS3244.1.
RefSeqiNP_114436.1. NM_032047.4.
XP_005247880.1. XM_005247823.4.
XP_005247881.1. XM_005247824.3.
XP_005247882.1. XM_005247825.3.
XP_011511527.1. XM_011513225.2.
XP_011511528.1. XM_011513226.2.
XP_011511529.1. XM_011513227.2.
XP_011511530.1. XM_011513228.2.
XP_011511531.1. XM_011513229.2.
UniGeneiHs.718506.

Genome annotation databases

EnsembliENST00000326505; ENSP00000316173; ENSG00000176597.
ENST00000460419; ENSP00000420778; ENSG00000176597.
ENST00000465010; ENSP00000417868; ENSG00000176597.
GeneIDi84002.
KEGGihsa:84002.
UCSCiuc003flk.3. human.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Beta1,3-N-acetylglucosaminyltransferase 5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB045278 mRNA. Translation: BAB40940.1.
AF368169 mRNA. Translation: AAK53403.1.
AJ304505 mRNA. Translation: CAC83093.1.
AB209517 mRNA. Translation: BAD92754.1. Frameshift.
CH471052 Genomic DNA. Translation: EAW78333.1.
CH471052 Genomic DNA. Translation: EAW78334.1.
CH471052 Genomic DNA. Translation: EAW78335.1.
CH471052 Genomic DNA. Translation: EAW78336.1.
BC028058 mRNA. Translation: AAH28058.1.
CCDSiCCDS3244.1.
RefSeqiNP_114436.1. NM_032047.4.
XP_005247880.1. XM_005247823.4.
XP_005247881.1. XM_005247824.3.
XP_005247882.1. XM_005247825.3.
XP_011511527.1. XM_011513225.2.
XP_011511528.1. XM_011513226.2.
XP_011511529.1. XM_011513227.2.
XP_011511530.1. XM_011513228.2.
XP_011511531.1. XM_011513229.2.
UniGeneiHs.718506.

3D structure databases

ProteinModelPortaliQ9BYG0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123846. 1 interaction.
IntActiQ9BYG0. 1 interaction.
STRINGi9606.ENSP00000316173.

Chemistry

SwissLipidsiSLP:000000768.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

PTM databases

PhosphoSiteiQ9BYG0.

Polymorphism and mutation databases

BioMutaiB3GNT5.
DMDMi74733473.

Proteomic databases

MaxQBiQ9BYG0.
PaxDbiQ9BYG0.
PeptideAtlasiQ9BYG0.
PRIDEiQ9BYG0.

Protocols and materials databases

DNASUi84002.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000326505; ENSP00000316173; ENSG00000176597.
ENST00000460419; ENSP00000420778; ENSG00000176597.
ENST00000465010; ENSP00000417868; ENSG00000176597.
GeneIDi84002.
KEGGihsa:84002.
UCSCiuc003flk.3. human.

Organism-specific databases

CTDi84002.
GeneCardsiB3GNT5.
HGNCiHGNC:15684. B3GNT5.
HPAiHPA017292.
MIMi615333. gene.
neXtProtiNX_Q9BYG0.
PharmGKBiPA25221.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2287. Eukaryota.
ENOG410ZZ1B. LUCA.
GeneTreeiENSGT00760000118879.
HOGENOMiHOG000064519.
HOVERGENiHBG101684.
InParanoidiQ9BYG0.
KOiK03766.
OMAiTWGNEKY.
OrthoDBiEOG091G0AXM.
PhylomeDBiQ9BYG0.
TreeFamiTF318639.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.206. 2681.
ReactomeiR-HSA-913709. O-linked glycosylation of mucins.

Miscellaneous databases

GenomeRNAii84002.
PROiQ9BYG0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000176597.
CleanExiHS_B3GNT5.
ExpressionAtlasiQ9BYG0. baseline and differential.
GenevisibleiQ9BYG0. HS.

Family and domain databases

InterProiIPR002659. Glyco_trans_31.
[Graphical view]
PANTHERiPTHR11214. PTHR11214. 1 hit.
PfamiPF01762. Galactosyl_T. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiB3GN5_HUMAN
AccessioniPrimary (citable) accession number: Q9BYG0
Secondary accession number(s): D3DNS5
, Q59FE3, Q7L9Z5, Q8WWP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.