Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase

Gene

B3GNT5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc4Cer; paragloboside), resulting in the synthesis of Lc3Cer and neolactopentaosylceramide (nLc5Cer), respectively. Probably plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • brain development Source: Ensembl
  • central nervous system development Source: UniProtKB
  • glycolipid biosynthetic process Source: UniProtKB
  • O-glycan processing Source: Reactome
  • protein glycosylation Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000176597-MONOMER
BRENDAi2.4.1.206 2681
ReactomeiR-HSA-913709 O-linked glycosylation of mucins
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT31 Glycosyltransferase Family 31

Chemistry databases

SwissLipidsiSLP:000000768

Names & Taxonomyi

Protein namesi
Recommended name:
Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase (EC:2.4.1.206)
Alternative name(s):
Lactotriaosylceramide synthase
Short name:
Lc(3)Cer synthase
Short name:
Lc3 synthase
UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5
Short name:
BGnT-5
Short name:
Beta-1,3-Gn-T5
Short name:
Beta-1,3-N-acetylglucosaminyltransferase 5
Short name:
Beta3Gn-T5
Gene namesi
Name:B3GNT5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000176597.11
HGNCiHGNC:15684 B3GNT5
MIMi615333 gene
neXtProtiNX_Q9BYG0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 14CytoplasmicSequence analysisAdd BLAST14
Transmembranei15 – 35Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini36 – 378LumenalSequence analysisAdd BLAST343

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi84002
OpenTargetsiENSG00000176597
PharmGKBiPA25221

Polymorphism and mutation databases

BioMutaiB3GNT5
DMDMi74733473

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002892091 – 378Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferaseAdd BLAST378

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi59N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9BYG0
PaxDbiQ9BYG0
PeptideAtlasiQ9BYG0
PRIDEiQ9BYG0

PTM databases

PhosphoSitePlusiQ9BYG0

Expressioni

Tissue specificityi

Widely expressed. Highly expressed in lung, colon, placenta, testis, pituitary gland and cerebellum. Weakly expressed in brain, liver, spleen, lymph node and thymus.1 Publication

Inductioni

Up-regulated by stimulation with retinoic acid and down-regulated with 12-O-tetradecanoylphorbol-13-acetate (TPA).1 Publication

Gene expression databases

BgeeiENSG00000176597
CleanExiHS_B3GNT5
ExpressionAtlasiQ9BYG0 baseline and differential
GenevisibleiQ9BYG0 HS

Organism-specific databases

HPAiHPA017292

Interactioni

Protein-protein interaction databases

BioGridi123846, 1 interactor
IntActiQ9BYG0, 2 interactors
STRINGi9606.ENSP00000316173

Structurei

3D structure databases

ProteinModelPortaliQ9BYG0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2287 Eukaryota
ENOG410ZZ1B LUCA
GeneTreeiENSGT00760000118879
HOGENOMiHOG000064519
HOVERGENiHBG101684
InParanoidiQ9BYG0
KOiK03766
OMAiTWGNEKY
OrthoDBiEOG091G0AXM
PhylomeDBiQ9BYG0
TreeFamiTF318639

Family and domain databases

InterProiView protein in InterPro
IPR002659 Glyco_trans_31
PANTHERiPTHR11214 PTHR11214, 1 hit
PfamiView protein in Pfam
PF01762 Galactosyl_T, 1 hit

Sequencei

Sequence statusi: Complete.

Q9BYG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMLVSGRRV KKWQLIIQLF ATCFLASLMF FWEPIDNHIV SHMKSYSYRY
60 70 80 90 100
LINSYDFVND TLSLKHTSAG PRYQYLINHK EKCQAQDVLL LLFVKTAPEN
110 120 130 140 150
YDRRSGIRRT WGNENYVRSQ LNANIKTLFA LGTPNPLEGE ELQRKLAWED
160 170 180 190 200
QRYNDIIQQD FVDSFYNLTL KLLMQFSWAN TYCPHAKFLM TADDDIFIHM
210 220 230 240 250
PNLIEYLQSL EQIGVQDFWI GRVHRGAPPI RDKSSKYYVS YEMYQWPAYP
260 270 280 290 300
DYTAGAAYVI SGDVAAKVYE ASQTLNSSLY IDDVFMGLCA NKIGIVPQDH
310 320 330 340 350
VFFSGEGKTP YHPCIYEKMM TSHGHLEDLQ DLWKNATDPK VKTISKGFFG
360 370
QIYCRLMKII LLCKISYVDT YPCRAAFI
Length:378
Mass (Da):44,053
Last modified:June 1, 2001 - v1
Checksum:iE19BA966A5E12CD2
GO

Sequence cautioni

The sequence BAD92754 differs from that shown. Reason: Frameshift at position 347.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB045278 mRNA Translation: BAB40940.1
AF368169 mRNA Translation: AAK53403.1
AJ304505 mRNA Translation: CAC83093.1
AB209517 mRNA Translation: BAD92754.1 Frameshift.
CH471052 Genomic DNA Translation: EAW78333.1
CH471052 Genomic DNA Translation: EAW78334.1
CH471052 Genomic DNA Translation: EAW78335.1
CH471052 Genomic DNA Translation: EAW78336.1
BC028058 mRNA Translation: AAH28058.1
CCDSiCCDS3244.1
RefSeqiNP_114436.1, NM_032047.4
XP_005247880.1, XM_005247823.4
XP_005247881.1, XM_005247824.3
XP_005247882.1, XM_005247825.3
XP_011511527.1, XM_011513225.2
XP_011511528.1, XM_011513226.2
XP_011511529.1, XM_011513227.2
XP_011511530.1, XM_011513228.2
XP_011511531.1, XM_011513229.2
XP_016862798.1, XM_017007309.1
UniGeneiHs.718506

Genome annotation databases

EnsembliENST00000326505; ENSP00000316173; ENSG00000176597
ENST00000460419; ENSP00000420778; ENSG00000176597
ENST00000465010; ENSP00000417868; ENSG00000176597
GeneIDi84002
KEGGihsa:84002
UCSCiuc003flk.3 human

Similar proteinsi

Entry informationi

Entry nameiB3GN5_HUMAN
AccessioniPrimary (citable) accession number: Q9BYG0
Secondary accession number(s): D3DNS5
, Q59FE3, Q7L9Z5, Q8WWP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 1, 2001
Last modified: February 28, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health