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Protein

Dual specificity protein phosphatase 16

Gene

DUSP16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dual specificity protein phosphatase involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2.2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei244Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • MAP kinase tyrosine/serine/threonine phosphatase activity Source: InterPro
  • phosphatase activity Source: UniProtKB
  • phosphoprotein phosphatase activity Source: UniProtKB
  • protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  • dephosphorylation Source: UniProtKB
  • inactivation of MAPK activity Source: UniProtKB
  • MAPK export from nucleus Source: UniProtKB
  • MAPK phosphatase export from nucleus, leptomycin B sensitive Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

BioCyciZFISH:HS03392-MONOMER.
ReactomeiR-HSA-112409. RAF-independent MAPK1/3 activation.
R-HSA-5675221. Negative regulation of MAPK pathway.
SignaLinkiQ9BY84.
SIGNORiQ9BY84.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 16 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
Mitogen-activated protein kinase phosphatase 7
Short name:
MAP kinase phosphatase 7
Short name:
MKP-7
Gene namesi
Name:DUSP16
Synonyms:KIAA1700, MKP7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:17909. DUSP16.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytoplasmic, membrane-bounded vesicle Source: UniProtKB-SubCell
  • cytosol Source: Ensembl
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi80824.
OpenTargetsiENSG00000111266.
ENSG00000280962.
PharmGKBiPA38475.

Polymorphism and mutation databases

BioMutaiDUSP16.
DMDMi20137933.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000948261 – 665Dual specificity protein phosphatase 16Add BLAST665

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei446Phosphoserine; by MAPK11 Publication1
Modified residuei501PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Ser-446 by MAPK1/ERK2, which prevents its degradation, and thereby stabilizes it and blocks JNK MAPK activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9BY84.
MaxQBiQ9BY84.
PaxDbiQ9BY84.
PeptideAtlasiQ9BY84.
PRIDEiQ9BY84.

PTM databases

DEPODiQ9BY84.
iPTMnetiQ9BY84.
PhosphoSitePlusiQ9BY84.

Expressioni

Gene expression databases

BgeeiENSG00000111266.
CleanExiHS_DUSP16.
ExpressionAtlasiQ9BY84. baseline and differential.
GenevisibleiQ9BY84. HS.

Organism-specific databases

HPAiHPA020326.

Interactioni

Subunit structurei

Interacts with ARRB2.1 Publication

Protein-protein interaction databases

BioGridi123321. 8 interactors.
IntActiQ9BY84. 2 interactors.
STRINGi9606.ENSP00000228862.

Structurei

Secondary structure

1665
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 10Combined sources3
Helixi12 – 19Combined sources8
Beta strandi21 – 24Combined sources4
Beta strandi26 – 30Combined sources5
Helixi34 – 39Combined sources6
Helixi52 – 59Combined sources8
Helixi65 – 71Combined sources7
Beta strandi83 – 88Combined sources6
Helixi95 – 97Combined sources3
Helixi103 – 114Combined sources12
Beta strandi118 – 121Combined sources4
Helixi124 – 131Combined sources8
Helixi133 – 135Combined sources3
Beta strandi160 – 163Combined sources4
Beta strandi166 – 170Combined sources5
Helixi171 – 175Combined sources5
Helixi177 – 183Combined sources7
Beta strandi187 – 190Combined sources4
Helixi203 – 205Combined sources3
Beta strandi206 – 208Combined sources3
Beta strandi213 – 216Combined sources4
Helixi223 – 235Combined sources13
Beta strandi240 – 249Combined sources10
Helixi250 – 262Combined sources13
Helixi267 – 277Combined sources11
Helixi285 – 297Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VSWX-ray2.20A/B5-150[»]
3TG3X-ray2.68A/B/C/D5-138[»]
4YR8X-ray2.40B/D/G/H156-301[»]
ProteinModelPortaliQ9BY84.
SMRiQ9BY84.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9BY84.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 137RhodanesePROSITE-ProRule annotationAdd BLAST116
Domaini227 – 289Tyrosine-protein phosphataseAdd BLAST63

Sequence similaritiesi

Contains 1 rhodanese domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00760000118902.
HOGENOMiHOG000082452.
HOVERGENiHBG005541.
InParanoidiQ9BY84.
KOiK04459.
OMAiQMEFGES.
OrthoDBiEOG091G0249.
PhylomeDBiQ9BY84.
TreeFamiTF105122.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.40.250.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR008343. MKP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR001763. Rhodanese-like_dom.
IPR016130. Tyr_Pase_AS.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR01764. MAPKPHPHTASE.
SMARTiSM00195. DSPc. 1 hit.
SM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BY84-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAHEMIGTQI VTERLVALLE SGTEKVLLID SRPFVEYNTS HILEAININC
60 70 80 90 100
SKLMKRRLQQ DKVLITELIQ HSAKHKVDID CSQKVVVYDQ SSQDVASLSS
110 120 130 140 150
DCFLTVLLGK LEKSFNSVHL LAGGFAEFSR CFPGLCEGKS TLVPTCISQP
160 170 180 190 200
CLPVANIGPT RILPNLYLGC QRDVLNKELM QQNGIGYVLN ASNTCPKPDF
210 220 230 240 250
IPESHFLRVP VNDSFCEKIL PWLDKSVDFI EKAKASNGCV LVHCLAGISR
260 270 280 290 300
SATIAIAYIM KRMDMSLDEA YRFVKEKRPT ISPNFNFLGQ LLDYEKKIKN
310 320 330 340 350
QTGASGPKSK LKLLHLEKPN EPVPAVSEGG QKSETPLSPP CADSATSEAA
360 370 380 390 400
GQRPVHPASV PSVPSVQPSL LEDSPLVQAL SGLHLSADRL EDSNKLKRSF
410 420 430 440 450
SLDIKSVSYS ASMAASLHGF SSSEDALEYY KPSTTLDGTN KLCQFSPVQE
460 470 480 490 500
LSEQTPETSP DKEEASIPKK LQTARPSDSQ SKRLHSVRTS SSGTAQRSLL
510 520 530 540 550
SPLHRSGSVE DNYHTSFLFG LSTSQQHLTK SAGLGLKGWH SDILAPQTST
560 570 580 590 600
PSLTSSWYFA TESSHFYSAS AIYGGSASYS AYSCSQLPTC GDQVYSVRRR
610 620 630 640 650
QKPSDRADSR RSWHEESPFE KQFKRRSCQM EFGESIMSEN RSREELGKVG
660
SQSSFSGSME IIEVS
Length:665
Mass (Da):73,102
Last modified:June 1, 2001 - v1
Checksum:i1BD853FF08460DFF
GO
Isoform 2 (identifier: Q9BY84-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     124-143: GFAEFSRCFPGLCEGKSTLV → ADAAEWDWLCVKCQQYLSKA
     144-665: Missing.

Note: No experimental confirmation available.
Show »
Length:143
Mass (Da):16,078
Checksum:i5213A213AA7E5974
GO

Sequence cautioni

The sequence BAB21791 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05175323T → M.Corresponds to variant rs36049447dbSNPEnsembl.1
Natural variantiVAR_051754366V → M.Corresponds to variant rs3809199dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056981124 – 143GFAEF…KSTLV → ADAAEWDWLCVKCQQYLSKA in isoform 2. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_056982144 – 665Missing in isoform 2. 1 PublicationAdd BLAST522

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052156 mRNA. Translation: BAB40814.1.
AF506796 mRNA. Translation: AAN75120.1.
AY038927 mRNA. Translation: AAK69770.1.
AB051487 mRNA. Translation: BAB21791.1. Different initiation.
AC007619 Genomic DNA. No translation available.
AC092824 Genomic DNA. No translation available.
BC109235 mRNA. Translation: AAI09236.1.
CCDSiCCDS8650.1. [Q9BY84-1]
RefSeqiNP_085143.1. NM_030640.2. [Q9BY84-1]
XP_006719218.1. XM_006719155.2. [Q9BY84-1]
XP_011519158.1. XM_011520856.1. [Q9BY84-1]
XP_011519159.1. XM_011520857.1. [Q9BY84-1]
UniGeneiHs.536535.

Genome annotation databases

EnsembliENST00000228862; ENSP00000228862; ENSG00000111266. [Q9BY84-2]
ENST00000298573; ENSP00000298573; ENSG00000111266. [Q9BY84-1]
ENST00000626413; ENSP00000487512; ENSG00000280962. [Q9BY84-1]
ENST00000628303; ENSP00000487034; ENSG00000280962. [Q9BY84-2]
GeneIDi80824.
KEGGihsa:80824.
UCSCiuc001rao.3. human. [Q9BY84-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052156 mRNA. Translation: BAB40814.1.
AF506796 mRNA. Translation: AAN75120.1.
AY038927 mRNA. Translation: AAK69770.1.
AB051487 mRNA. Translation: BAB21791.1. Different initiation.
AC007619 Genomic DNA. No translation available.
AC092824 Genomic DNA. No translation available.
BC109235 mRNA. Translation: AAI09236.1.
CCDSiCCDS8650.1. [Q9BY84-1]
RefSeqiNP_085143.1. NM_030640.2. [Q9BY84-1]
XP_006719218.1. XM_006719155.2. [Q9BY84-1]
XP_011519158.1. XM_011520856.1. [Q9BY84-1]
XP_011519159.1. XM_011520857.1. [Q9BY84-1]
UniGeneiHs.536535.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VSWX-ray2.20A/B5-150[»]
3TG3X-ray2.68A/B/C/D5-138[»]
4YR8X-ray2.40B/D/G/H156-301[»]
ProteinModelPortaliQ9BY84.
SMRiQ9BY84.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123321. 8 interactors.
IntActiQ9BY84. 2 interactors.
STRINGi9606.ENSP00000228862.

PTM databases

DEPODiQ9BY84.
iPTMnetiQ9BY84.
PhosphoSitePlusiQ9BY84.

Polymorphism and mutation databases

BioMutaiDUSP16.
DMDMi20137933.

Proteomic databases

EPDiQ9BY84.
MaxQBiQ9BY84.
PaxDbiQ9BY84.
PeptideAtlasiQ9BY84.
PRIDEiQ9BY84.

Protocols and materials databases

DNASUi80824.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000228862; ENSP00000228862; ENSG00000111266. [Q9BY84-2]
ENST00000298573; ENSP00000298573; ENSG00000111266. [Q9BY84-1]
ENST00000626413; ENSP00000487512; ENSG00000280962. [Q9BY84-1]
ENST00000628303; ENSP00000487034; ENSG00000280962. [Q9BY84-2]
GeneIDi80824.
KEGGihsa:80824.
UCSCiuc001rao.3. human. [Q9BY84-1]

Organism-specific databases

CTDi80824.
DisGeNETi80824.
GeneCardsiDUSP16.
HGNCiHGNC:17909. DUSP16.
HPAiHPA020326.
MIMi607175. gene.
neXtProtiNX_Q9BY84.
OpenTargetsiENSG00000111266.
ENSG00000280962.
PharmGKBiPA38475.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00760000118902.
HOGENOMiHOG000082452.
HOVERGENiHBG005541.
InParanoidiQ9BY84.
KOiK04459.
OMAiQMEFGES.
OrthoDBiEOG091G0249.
PhylomeDBiQ9BY84.
TreeFamiTF105122.

Enzyme and pathway databases

BioCyciZFISH:HS03392-MONOMER.
ReactomeiR-HSA-112409. RAF-independent MAPK1/3 activation.
R-HSA-5675221. Negative regulation of MAPK pathway.
SignaLinkiQ9BY84.
SIGNORiQ9BY84.

Miscellaneous databases

ChiTaRSiDUSP16. human.
EvolutionaryTraceiQ9BY84.
GeneWikiiDUSP16.
GenomeRNAii80824.
PROiQ9BY84.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000111266.
CleanExiHS_DUSP16.
ExpressionAtlasiQ9BY84. baseline and differential.
GenevisibleiQ9BY84. HS.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.40.250.10. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR008343. MKP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR001763. Rhodanese-like_dom.
IPR016130. Tyr_Pase_AS.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR01764. MAPKPHPHTASE.
SMARTiSM00195. DSPc. 1 hit.
SM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
SSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDUS16_HUMAN
AccessioniPrimary (citable) accession number: Q9BY84
Secondary accession number(s): Q547C7, Q96QS2, Q9C0G3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.