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Protein

E3 ubiquitin-protein ligase RNF26

Gene

RNF26

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that plays a key role in endosome organization by retaining vesicles in the perinuclear cloud (PubMed:27368102). Acts as a platform for perinuclear positioning of the endosomal system by mediating ubiquitination of SQSTM1 (PubMed:27368102). Ubiquitinated SQSTM1 attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed:27368102). Also acts as a regulator of type I interferon production in response to viral infection by mediating the formation of 'Lys-11'-linked polyubiquitin chains on TMEM173/STING, leading to stabilize TMEM173/STING (PubMed:25254379). Also required to limit type I interferon response by promoting autophagic degradation of IRF3 (PubMed:25254379).2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.2 Publications
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri380 – 422RING-typePROSITE-ProRule annotationAdd BLAST43

GO - Molecular functioni

  • ubiquitin protein ligase activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • endosome organization Source: UniProtKB
  • negative regulation of defense response to virus Source: UniProtKB
  • protein K11-linked ubiquitination Source: UniProtKB
  • protein localization to perinuclear region of cytoplasm Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
  • regulation of type I interferon production Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF26Curated (EC:2.3.2.272 Publications)
Alternative name(s):
RING finger protein 26Curated
Gene namesi
Name:RNF26Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:14646. RNF26.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei24 – 44HelicalSequence analysisAdd BLAST21
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Transmembranei147 – 169HelicalSequence analysisAdd BLAST23
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Transmembranei220 – 240HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi382I → R: Strongly decreased E3 ubiquitin-protein ligase activity due to impaired interaction with E2 enzymes. Impaired ability to retain vesicles in the perinuclear cloud. 1 Publication1
Mutagenesisi395C → S: Strongly decreased E3 ubiquitin-protein ligase activity. Impaired ability to mediate ubiquitination of TMEM173/STING. 1 Publication1
Mutagenesisi399C → S: Strongly decreased E3 ubiquitin-protein ligase activity. Impaired ability to mediate ubiquitination of TMEM173/STING. 1 Publication1
Mutagenesisi401C → S: Strongly decreased E3 ubiquitin-protein ligase activity. Impaired ability to mediate ubiquitination of TMEM173/STING. 1 Publication1

Organism-specific databases

DisGeNETi79102.
OpenTargetsiENSG00000173456.
PharmGKBiPA34430.

Polymorphism and mutation databases

BioMutaiRNF26.
DMDMi20139692.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000560681 – 433E3 ubiquitin-protein ligase RNF26Add BLAST433

Proteomic databases

EPDiQ9BY78.
PaxDbiQ9BY78.
PeptideAtlasiQ9BY78.
PRIDEiQ9BY78.

PTM databases

iPTMnetiQ9BY78.
PhosphoSitePlusiQ9BY78.

Expressioni

Tissue specificityi

Ubiquitous. Up-regulated in several cancer cell lines.1 Publication

Gene expression databases

BgeeiENSG00000173456.
CleanExiHS_RNF26.
GenevisibleiQ9BY78. HS.

Organism-specific databases

HPAiHPA027620.

Interactioni

Protein-protein interaction databases

BioGridi122549. 27 interactors.
IntActiQ9BY78. 9 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ9BY78.
SMRiQ9BY78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri380 – 422RING-typePROSITE-ProRule annotationAdd BLAST43

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITZ8. Eukaryota.
ENOG410XVCP. LUCA.
GeneTreeiENSGT00390000016584.
HOGENOMiHOG000136849.
HOVERGENiHBG054207.
InParanoidiQ9BY78.
KOiK15693.
OMAiCRRGILQ.
OrthoDBiEOG091G195F.
PhylomeDBiQ9BY78.
TreeFamiTF331813.

Family and domain databases

CDDicd00162. RING. 1 hit.
InterProiView protein in InterPro
IPR001841. Znf_RING.
PROSITEiView protein in PROSITE
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9BY78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAVYLVVNG LGLVLDVLTL VLDLNFLLVS SLLASLAWLL AFVYNLPHTV
60 70 80 90 100
LTSLLHLGRG VLLSLLALIE AVVRFTCGGL QALCTLLYSC CSGLESLKLL
110 120 130 140 150
GHLASHGALR SREILHRGVL NVVSSGHALL RQACDICAIA MSLVAYVINS
160 170 180 190 200
LVNICLIGTQ NLFSLVLALW DAVTGPLWRM TDVVAAFLAH ISSSAVAMAI
210 220 230 240 250
LLWTPCQLAL ELLASAARLL ASFVLVNLTG LVLLACVLAV TVTVLHPDFT
260 270 280 290 300
LRLATQALSQ LHARPSYHRL REDVMRLSRL ALGSEAWRRV WSRSLQLASW
310 320 330 340 350
PNRGGAPGAP QGDPMRVFSV RTRRQDTLPE AGRRSEAEEE EARTIRVTPV
360 370 380 390 400
RGRERLNEEE PPGGQDPWKL LKEQEERKKC VICQDQSKTV LLLPCRHLCL
410 420 430
CQACTEILMR HPVYHRNCPL CRRGILQTLN VYL
Length:433
Mass (Da):47,737
Last modified:June 1, 2001 - v1
Checksum:iC23E36A66C3677E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB055622 mRNA. Translation: BAB40955.1.
AK075379 mRNA. Translation: BAC11580.1.
CH471065 Genomic DNA. Translation: EAW67479.1.
BC000058 mRNA. Translation: AAH00058.1.
BC007534 mRNA. Translation: AAH07534.1.
CCDSiCCDS8419.1.
PIRiJC7678.
RefSeqiNP_114404.1. NM_032015.4.
UniGeneiHs.524084.
Hs.744159.
Hs.745522.

Genome annotation databases

EnsembliENST00000311413; ENSP00000312439; ENSG00000173456.
GeneIDi79102.
KEGGihsa:79102.
UCSCiuc001pwh.4. human.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRNF26_HUMAN
AccessioniPrimary (citable) accession number: Q9BY78
Secondary accession number(s): Q542Y8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: June 1, 2001
Last modified: July 5, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways