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Protein

Polymerase delta-interacting protein 3

Gene

POLDIP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Involved in nuclear mRNA export; probably mediated by assoociation with the TREX complex.2 Publications

GO - Molecular functioni

GO - Biological processi

  • mRNA 3'-end processing Source: Reactome
  • mRNA export from nucleus Source: Reactome
  • poly(A)+ mRNA export from nucleus Source: UniProtKB
  • positive regulation of translation Source: UniProtKB
  • RNA export from nucleus Source: Reactome

Keywordsi

Molecular functionRNA-binding
Biological processmRNA transport, Translation regulation, Transport

Enzyme and pathway databases

ReactomeiR-HSA-109688 Cleavage of Growing Transcript in the Termination Region
R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-HSA-72187 mRNA 3'-end processing
SIGNORiQ9BY77

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase delta-interacting protein 3
Alternative name(s):
46 kDa DNA polymerase delta interaction protein
Short name:
p46
S6K1 Aly/REF-like target
Short name:
SKAR
Gene namesi
Name:POLDIP3
Synonyms:KIAA1649, PDIP46
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000100227.17
HGNCiHGNC:23782 POLDIP3
MIMi611520 gene
neXtProtiNX_Q9BY77

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi383S → A: Reduces in vitro phosphorylation by RPS6KB1. Abolishes in vitro phosphorylation by RPS6KB1; when associated with A-385. 1 Publication1
Mutagenesisi385S → A: Reduces in vitro phosphorylation by RPS6KB1. Reduces in vitro phosphorylation by RPS6KB1; when associated with A-383. 1 Publication1

Organism-specific databases

DisGeNETi84271
OpenTargetsiENSG00000100227
PharmGKBiPA134899124

Polymorphism and mutation databases

BioMutaiPOLDIP3
DMDMi50403796

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000817222 – 421Polymerase delta-interacting protein 3Add BLAST420

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei5PhosphoserineCombined sources1
Modified residuei33Omega-N-methylarginineCombined sources1
Modified residuei44PhosphoserineCombined sources1
Modified residuei127PhosphoserineCombined sources1 Publication1
Modified residuei140PhosphothreonineCombined sources1
Cross-linki200Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei204PhosphoserineCombined sources1
Modified residuei215PhosphoserineCombined sources1
Modified residuei217PhosphoserineCombined sources1
Cross-linki223Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei244PhosphoserineCombined sources1
Cross-linki248Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei275PhosphoserineCombined sources1 Publication1
Cross-linki372Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei383Phosphoserine; by RPS6KB11 Publication1
Modified residuei385Phosphoserine; by RPS6KB11 Publication1
Cross-linki418Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Phosphorylated at Ser-383 and Ser-385 by RPS6KB1.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9BY77
MaxQBiQ9BY77
PaxDbiQ9BY77
PeptideAtlasiQ9BY77
PRIDEiQ9BY77

PTM databases

iPTMnetiQ9BY77
PhosphoSitePlusiQ9BY77

Miscellaneous databases

PMAP-CutDBQ9BY77

Expressioni

Gene expression databases

BgeeiENSG00000100227
CleanExiHS_POLDIP3
ExpressionAtlasiQ9BY77 baseline and differential
GenevisibleiQ9BY77 HS

Organism-specific databases

HPAiHPA018419
HPA048790

Interactioni

Subunit structurei

Interacts with POLD2. Interacts with NCBP1 and EIF4A3. Associates with the multiprotein exon junction complex (EJC). Interacts with RPS6KB1 (activated). Interacts with ERH. Interacts with THOC2, DDX39B and ZC3H11A; the interactions are ATP-dependent and indicative for an association with the TREX complex.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NCBP1Q091613EBI-1776152,EBI-464743

Protein-protein interaction databases

BioGridi123998, 56 interactors
IntActiQ9BY77, 28 interactors
MINTiQ9BY77
STRINGi9606.ENSP00000252115

Structurei

3D structure databases

ProteinModelPortaliQ9BY77
SMRiQ9BY77
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini280 – 351RRMPROSITE-ProRule annotationAdd BLAST72

Phylogenomic databases

eggNOGiKOG0533 Eukaryota
ENOG4111JAW LUCA
GeneTreeiENSGT00390000018868
HOVERGENiHBG053550
InParanoidiQ9BY77
KOiK22414
PhylomeDBiQ9BY77
TreeFamiTF313312

Family and domain databases

CDDicd12681 RRM_SKAR, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR034785 PDIP3
IPR034784 PDIP3_RRM
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PANTHERiPTHR19965:SF34 PTHR19965:SF34, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BY77-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADISLDELI RKRGAAAKGR LNARPGVGGV RSRVGIQQGL LSQSTRTATF
60 70 80 90 100
QQRFDARQKI GLSDARLKLG VKDAREKLLQ KDARFRIKGK VQDAREMLNS
110 120 130 140 150
RKQQTTVPQK PRQVADAREK ISLKRSSPAA FINPPIGTVT PALKLTKTIQ
160 170 180 190 200
VPQQKAMAPL HPHPAGMRIN VVNNHQAKQN LYDLDEDDDG IASVPTKQMK
210 220 230 240 250
FAASGGFLHH MAGLSSSKLS MSKALPLTKV VQNDAYTAPA LPSSIRTKAL
260 270 280 290 300
TNMSRTLVNK EEPPKELPAA EPVLSPLEGT KMTVNNLHPR VTEEDIVELF
310 320 330 340 350
CVCGALKRAR LVHPGVAEVV FVKKDDAITA YKKYNNRCLD GQPMKCNLHM
360 370 380 390 400
NGNVITSDQP ILLRLSDSPS MKKESELPRR VNSASSSNPP AEVDPDTILK
410 420
ALFKSSGASV TTQPTEFKIK L
Length:421
Mass (Da):46,089
Last modified:July 19, 2004 - v2
Checksum:i39A4A2362852E020
GO
Isoform 2 (identifier: Q9BY77-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     151-179: Missing.

Show »
Length:392
Mass (Da):42,895
Checksum:i79AA8DA00FD5D6C6
GO

Sequence cautioni

The sequence BAB33368 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011056151 – 179Missing in isoform 2. 3 PublicationsAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY422990 Genomic DNA Translation: AAQ94604.1
AB055760 mRNA Translation: BAB33368.2 Different initiation.
AL160111 mRNA Translation: CAB77058.1
AL160112 mRNA Translation: CAB77059.1
CR456456 mRNA Translation: CAG30342.1
AK291623 mRNA Translation: BAF84312.1
AK291774 mRNA Translation: BAF84463.1
Z93241 Genomic DNA No translation available.
BC049840 mRNA Translation: AAH49840.1
BC056912 mRNA Translation: AAH56912.2
BC095411 mRNA Translation: AAH95411.1
DQ887818 mRNA Translation: ABI74675.1
CCDSiCCDS14038.1 [Q9BY77-1]
CCDS14039.1 [Q9BY77-2]
RefSeqiNP_115687.2, NM_032311.4 [Q9BY77-1]
NP_835237.1, NM_178136.2 [Q9BY77-2]
UniGeneiHs.505802

Genome annotation databases

EnsembliENST00000252115; ENSP00000252115; ENSG00000100227 [Q9BY77-1]
ENST00000348657; ENSP00000252116; ENSG00000100227 [Q9BY77-2]
GeneIDi84271
KEGGihsa:84271
UCSCiuc003bcu.4 human [Q9BY77-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPDIP3_HUMAN
AccessioniPrimary (citable) accession number: Q9BY77
Secondary accession number(s): A8K6F8
, A8K6V9, Q009A7, Q5H972, Q6PGN6, Q7Z6Z0, Q9NSP5, Q9NSP6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: July 19, 2004
Last modified: May 23, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health