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Q9BY64 (UDB28_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-glucuronosyltransferase 2B28

Short name=UDPGT 2B28
EC=2.4.1.17
Gene names
Name:UGT2B28
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length529 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme has glucuronidating capacity with steroid substrates such as 5-beta-androstane 3-alpha,17-beta-diol, estradiol, ADT, eugenol and bile acids. Only isoform 1 seems to be active. Ref.1

Catalytic activity

UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside. Ref.1

Subcellular location

Microsome membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein Ref.1.

Tissue specificity

Expressed in the liver, breast and kidney. Ref.1

Sequence similarities

Belongs to the UDP-glycosyltransferase family.

Sequence caution

The sequence AAK31809.1 differs from that shown. Reason: Aberrant splicing.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9BY64-1)

Also known as: I;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9BY64-2)

Also known as: II;

The sequence of this isoform differs from the canonical sequence as follows:
     335-335: V → I
     336-529: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 529505UDP-glucuronosyltransferase 2B28
PRO_0000036046

Regions

Transmembrane495 – 51723Helical; Potential

Amino acid modifications

Glycosylation3151N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence3351V → I in isoform 2.
VSP_006710
Alternative sequence336 – 529194Missing in isoform 2.
VSP_006711
Natural variant3651L → H. Ref.2
Corresponds to variant rs4235127 [ dbSNP | Ensembl ].
VAR_059847
Natural variant4471I → R. Ref.2
Corresponds to variant rs6843900 [ dbSNP | Ensembl ].
VAR_060661
Natural variant4581H → D. Ref.2
Corresponds to variant rs6828191 [ dbSNP | Ensembl ].
VAR_060662

Experimental info

Sequence conflict1731C → R in BAG70270. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (I) [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 8C75277E964690C1

FASTA52960,906
        10         20         30         40         50         60 
MALKWTSVLL LIHLGCYFSS GSCGKVLVWT GEYSHWMNMK TILKELVQRG HEVTVLASSA 

        70         80         90        100        110        120 
SILFDPNDAF TLKLEVYPTS LTKTEFENII MQQVKRWSDI QKDSFWLYFS QEQEILWEFH 

       130        140        150        160        170        180 
DIFRNFCKDV VSNKKVMKKL QESRFDIIFA DAFFPCGELL AALLNIPFVY SLCFTPGYTI 

       190        200        210        220        230        240 
ERHSGGLIFP PSYIPVVMSK LSDQMTFMER VKNMIYVLYF DFWFQMCDMK KWDQFYSEVL 

       250        260        270        280        290        300 
GRPTTLFETM GKADIWLMRN SWSFQFPHPF LPNIDFVGGL HCKPAKPLPK EMEEFVQSSG 

       310        320        330        340        350        360 
ENGVVVFSLG SVISNMTAER ANVIATALAK IPQKVLWRFD GNKPDALGLN TRLYKWIPQN 

       370        380        390        400        410        420 
DLLGLPKTRA FITHGGANGI YEAIYHGIPM VGIPLFWDQP DNIAHMKAKG AAVRLDFHTM 

       430        440        450        460        470        480 
SSTDLLNALK TVINDPSYKE NVMKLSIIQH DQPVKPLHRA VFWIEFVMCH KGAKHLRVAA 

       490        500        510        520 
RDLTWFQYHS LDVIGFLLAC VATVIFVVTK FCLFCFWKFA RKGKKGKRD 

« Hide

Isoform 2 (II) [UniParc].

Checksum: D136BAE9B6A34539
Show »

FASTA33538,743

References

« Hide 'large scale' references
[1]"Isolation and characterization of the UGT2B28 cDNA encoding a novel human steroid conjugating UDP-glucuronosyltransferase."
Levesque E., Turgeon D., Carrier J.-S., Montminy V., Beaulieu M., Belanger A.
Biochemistry 40:3869-3881(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B. expand/collapse author list , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N.
Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS HIS-365; ARG-447 AND ASP-458.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF177272 mRNA. Translation: AAK31807.1.
AF177273 mRNA. Translation: AAK31808.1.
AF177274 mRNA. Translation: AAK31809.1. Sequence problems.
AB451456 mRNA. Translation: BAG70270.1.
CCDSCCDS3528.1. [Q9BY64-1]
CCDS56330.1. [Q9BY64-2]
RefSeqNP_001193933.1. NM_001207004.1. [Q9BY64-2]
NP_444267.1. NM_053039.1. [Q9BY64-1]
XP_005265754.1. XM_005265697.1.
UniGeneHs.653154.

3D structure databases

ProteinModelPortalQ9BY64.
SMRQ9BY64. Positions 285-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000334276.

Chemistry

ChEMBLCHEMBL6189.
DrugBankDB01544. Flunitrazepam.

Protein family/group databases

CAZyGT1. Glycosyltransferase Family 1.

PTM databases

PhosphoSiteQ9BY64.

Polymorphism databases

DMDM20140759.

Proteomic databases

MaxQBQ9BY64.
PaxDbQ9BY64.
PRIDEQ9BY64.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000335568; ENSP00000334276; ENSG00000135226. [Q9BY64-1]
ENST00000511240; ENSP00000427399; ENSG00000135226. [Q9BY64-2]
GeneID54490.
KEGGhsa:54490.
UCSCuc003hej.3. human. [Q9BY64-1]
uc010ihr.3. human. [Q9BY64-2]

Organism-specific databases

CTD54490.
GeneCardsGC04P070194.
H-InvDBHIX0031374.
HGNCHGNC:13479. UGT2B28.
HPAHPA045108.
MIM606497. gene.
neXtProtNX_Q9BY64.
PharmGKBPA37779.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1819.
HOGENOMHOG000220831.
HOVERGENHBG004033.
InParanoidQ9BY64.
KOK00699.
OMAKRWSDIQ.
OrthoDBEOG7GBFWS.
PhylomeDBQ9BY64.
TreeFamTF315472.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.

Gene expression databases

BgeeQ9BY64.
CleanExHS_UGT2B28.
GenevestigatorQ9BY64.

Family and domain databases

InterProIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERPTHR11926. PTHR11926. 1 hit.
PfamPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi54490.
NextBio56811.
PROQ9BY64.
SOURCESearch...

Entry information

Entry nameUDB28_HUMAN
AccessionPrimary (citable) accession number: Q9BY64
Secondary accession number(s): B5BUM0, Q9BY62, Q9BY63
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM