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Protein

UDP-glucuronosyltransferase 2B28

Gene

UGT2B28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme has glucuronidating capacity with steroid substrates such as 5-beta-androstane 3-alpha,17-beta-diol, estradiol, ADT, eugenol and bile acids. Only isoform 1 seems to be active.1 Publication

Catalytic activityi

UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.1 Publication

GO - Molecular functioni

  1. glucuronosyltransferase activity Source: GO_Central

GO - Biological processi

  1. cellular response to hormone stimulus Source: GO_Central
  2. flavonoid biosynthetic process Source: GO_Central
  3. flavonoid glucuronidation Source: GO_Central
  4. metabolic process Source: UniProtKB
  5. xenobiotic metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_6784. Glucuronidation.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucuronosyltransferase 2B28 (EC:2.4.1.17)
Short name:
UDPGT 2B28
Gene namesi
Name:UGT2B28
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:13479. UGT2B28.

Subcellular locationi

Microsome membrane 1 Publication; Single-pass membrane protein 1 Publication. Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei495 – 51723HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37779.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 529505UDP-glucuronosyltransferase 2B28PRO_0000036046Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9BY64.
PaxDbiQ9BY64.
PRIDEiQ9BY64.

PTM databases

PhosphoSiteiQ9BY64.

Expressioni

Tissue specificityi

Expressed in the liver, breast and kidney.1 Publication

Gene expression databases

BgeeiQ9BY64.
CleanExiHS_UGT2B28.
GenevestigatoriQ9BY64.

Organism-specific databases

HPAiHPA045108.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000334276.

Structurei

3D structure databases

ProteinModelPortaliQ9BY64.
SMRiQ9BY64. Positions 285-446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1819.
GeneTreeiENSGT00760000118949.
HOGENOMiHOG000220831.
HOVERGENiHBG004033.
InParanoidiQ9BY64.
KOiK00699.
OMAiKRWSDIQ.
OrthoDBiEOG7GBFWS.
PhylomeDBiQ9BY64.
TreeFamiTF315472.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9BY64-1) [UniParc]FASTAAdd to Basket

Also known as: I

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALKWTSVLL LIHLGCYFSS GSCGKVLVWT GEYSHWMNMK TILKELVQRG
60 70 80 90 100
HEVTVLASSA SILFDPNDAF TLKLEVYPTS LTKTEFENII MQQVKRWSDI
110 120 130 140 150
QKDSFWLYFS QEQEILWEFH DIFRNFCKDV VSNKKVMKKL QESRFDIIFA
160 170 180 190 200
DAFFPCGELL AALLNIPFVY SLCFTPGYTI ERHSGGLIFP PSYIPVVMSK
210 220 230 240 250
LSDQMTFMER VKNMIYVLYF DFWFQMCDMK KWDQFYSEVL GRPTTLFETM
260 270 280 290 300
GKADIWLMRN SWSFQFPHPF LPNIDFVGGL HCKPAKPLPK EMEEFVQSSG
310 320 330 340 350
ENGVVVFSLG SVISNMTAER ANVIATALAK IPQKVLWRFD GNKPDALGLN
360 370 380 390 400
TRLYKWIPQN DLLGLPKTRA FITHGGANGI YEAIYHGIPM VGIPLFWDQP
410 420 430 440 450
DNIAHMKAKG AAVRLDFHTM SSTDLLNALK TVINDPSYKE NVMKLSIIQH
460 470 480 490 500
DQPVKPLHRA VFWIEFVMCH KGAKHLRVAA RDLTWFQYHS LDVIGFLLAC
510 520
VATVIFVVTK FCLFCFWKFA RKGKKGKRD
Length:529
Mass (Da):60,906
Last modified:June 1, 2001 - v1
Checksum:i8C75277E964690C1
GO
Isoform 2 (identifier: Q9BY64-2) [UniParc]FASTAAdd to Basket

Also known as: II

The sequence of this isoform differs from the canonical sequence as follows:
     335-335: V → I
     336-529: Missing.

Show »
Length:335
Mass (Da):38,743
Checksum:iD136BAE9B6A34539
GO

Sequence cautioni

The sequence AAK31809.1 differs from that shown.Aberrant splicing.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731C → R in BAG70270. (PubMed:19054851)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti365 – 3651L → H.1 Publication
Corresponds to variant rs4235127 [ dbSNP | Ensembl ].
VAR_059847
Natural varianti447 – 4471I → R.1 Publication
Corresponds to variant rs6843900 [ dbSNP | Ensembl ].
VAR_060661
Natural varianti458 – 4581H → D.1 Publication
Corresponds to variant rs6828191 [ dbSNP | Ensembl ].
VAR_060662

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei335 – 3351V → I in isoform 2. 1 PublicationVSP_006710
Alternative sequencei336 – 529194Missing in isoform 2. 1 PublicationVSP_006711Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177272 mRNA. Translation: AAK31807.1.
AF177273 mRNA. Translation: AAK31808.1.
AF177274 mRNA. Translation: AAK31809.1. Sequence problems.
AB451456 mRNA. Translation: BAG70270.1.
CCDSiCCDS3528.1. [Q9BY64-1]
CCDS56330.1. [Q9BY64-2]
RefSeqiNP_001193933.1. NM_001207004.1. [Q9BY64-2]
NP_444267.1. NM_053039.1. [Q9BY64-1]
XP_005265754.1. XM_005265697.1.
UniGeneiHs.653154.

Genome annotation databases

EnsembliENST00000335568; ENSP00000334276; ENSG00000135226. [Q9BY64-1]
ENST00000511240; ENSP00000427399; ENSG00000135226. [Q9BY64-2]
GeneIDi54490.
KEGGihsa:54490.
UCSCiuc003hej.3. human. [Q9BY64-1]
uc010ihr.3. human. [Q9BY64-2]

Polymorphism databases

DMDMi20140759.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177272 mRNA. Translation: AAK31807.1.
AF177273 mRNA. Translation: AAK31808.1.
AF177274 mRNA. Translation: AAK31809.1. Sequence problems.
AB451456 mRNA. Translation: BAG70270.1.
CCDSiCCDS3528.1. [Q9BY64-1]
CCDS56330.1. [Q9BY64-2]
RefSeqiNP_001193933.1. NM_001207004.1. [Q9BY64-2]
NP_444267.1. NM_053039.1. [Q9BY64-1]
XP_005265754.1. XM_005265697.1.
UniGeneiHs.653154.

3D structure databases

ProteinModelPortaliQ9BY64.
SMRiQ9BY64. Positions 285-446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000334276.

Chemistry

ChEMBLiCHEMBL6189.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

PTM databases

PhosphoSiteiQ9BY64.

Polymorphism databases

DMDMi20140759.

Proteomic databases

MaxQBiQ9BY64.
PaxDbiQ9BY64.
PRIDEiQ9BY64.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000335568; ENSP00000334276; ENSG00000135226. [Q9BY64-1]
ENST00000511240; ENSP00000427399; ENSG00000135226. [Q9BY64-2]
GeneIDi54490.
KEGGihsa:54490.
UCSCiuc003hej.3. human. [Q9BY64-1]
uc010ihr.3. human. [Q9BY64-2]

Organism-specific databases

CTDi54490.
GeneCardsiGC04P070194.
H-InvDBHIX0031374.
HGNCiHGNC:13479. UGT2B28.
HPAiHPA045108.
MIMi606497. gene.
neXtProtiNX_Q9BY64.
PharmGKBiPA37779.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1819.
GeneTreeiENSGT00760000118949.
HOGENOMiHOG000220831.
HOVERGENiHBG004033.
InParanoidiQ9BY64.
KOiK00699.
OMAiKRWSDIQ.
OrthoDBiEOG7GBFWS.
PhylomeDBiQ9BY64.
TreeFamiTF315472.

Enzyme and pathway databases

ReactomeiREACT_6784. Glucuronidation.

Miscellaneous databases

GenomeRNAii54490.
NextBioi56811.
PROiQ9BY64.
SOURCEiSearch...

Gene expression databases

BgeeiQ9BY64.
CleanExiHS_UGT2B28.
GenevestigatoriQ9BY64.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the UGT2B28 cDNA encoding a novel human steroid conjugating UDP-glucuronosyltransferase."
    Levesque E., Turgeon D., Carrier J.-S., Montminy V., Beaulieu M., Belanger A.
    Biochemistry 40:3869-3881(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
    Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B.
    , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N.
    Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS HIS-365; ARG-447 AND ASP-458.

Entry informationi

Entry nameiUDB28_HUMAN
AccessioniPrimary (citable) accession number: Q9BY64
Secondary accession number(s): B5BUM0, Q9BY62, Q9BY63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: June 1, 2001
Last modified: February 4, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.