Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9BY64

- UDB28_HUMAN

UniProt

Q9BY64 - UDB28_HUMAN

Protein

UDP-glucuronosyltransferase 2B28

Gene

UGT2B28

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme has glucuronidating capacity with steroid substrates such as 5-beta-androstane 3-alpha,17-beta-diol, estradiol, ADT, eugenol and bile acids. Only isoform 1 seems to be active.1 Publication

    Catalytic activityi

    UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.1 Publication

    GO - Molecular functioni

    1. glucuronosyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. metabolic process Source: UniProtKB
    2. xenobiotic metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_6784. Glucuronidation.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucuronosyltransferase 2B28 (EC:2.4.1.17)
    Short name:
    UDPGT 2B28
    Gene namesi
    Name:UGT2B28
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:13479. UGT2B28.

    Subcellular locationi

    Microsome membrane 1 Publication; Single-pass membrane protein 1 Publication. Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA37779.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 529505UDP-glucuronosyltransferase 2B28PRO_0000036046Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ9BY64.
    PaxDbiQ9BY64.
    PRIDEiQ9BY64.

    PTM databases

    PhosphoSiteiQ9BY64.

    Expressioni

    Tissue specificityi

    Expressed in the liver, breast and kidney.1 Publication

    Gene expression databases

    BgeeiQ9BY64.
    CleanExiHS_UGT2B28.
    GenevestigatoriQ9BY64.

    Organism-specific databases

    HPAiHPA045108.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000334276.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9BY64.
    SMRiQ9BY64. Positions 285-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei495 – 51723HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the UDP-glycosyltransferase family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1819.
    HOGENOMiHOG000220831.
    HOVERGENiHBG004033.
    InParanoidiQ9BY64.
    KOiK00699.
    OMAiKRWSDIQ.
    OrthoDBiEOG7GBFWS.
    PhylomeDBiQ9BY64.
    TreeFamiTF315472.

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]
    PROSITEiPS00375. UDPGT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9BY64-1) [UniParc]FASTAAdd to Basket

    Also known as: I

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MALKWTSVLL LIHLGCYFSS GSCGKVLVWT GEYSHWMNMK TILKELVQRG    50
    HEVTVLASSA SILFDPNDAF TLKLEVYPTS LTKTEFENII MQQVKRWSDI 100
    QKDSFWLYFS QEQEILWEFH DIFRNFCKDV VSNKKVMKKL QESRFDIIFA 150
    DAFFPCGELL AALLNIPFVY SLCFTPGYTI ERHSGGLIFP PSYIPVVMSK 200
    LSDQMTFMER VKNMIYVLYF DFWFQMCDMK KWDQFYSEVL GRPTTLFETM 250
    GKADIWLMRN SWSFQFPHPF LPNIDFVGGL HCKPAKPLPK EMEEFVQSSG 300
    ENGVVVFSLG SVISNMTAER ANVIATALAK IPQKVLWRFD GNKPDALGLN 350
    TRLYKWIPQN DLLGLPKTRA FITHGGANGI YEAIYHGIPM VGIPLFWDQP 400
    DNIAHMKAKG AAVRLDFHTM SSTDLLNALK TVINDPSYKE NVMKLSIIQH 450
    DQPVKPLHRA VFWIEFVMCH KGAKHLRVAA RDLTWFQYHS LDVIGFLLAC 500
    VATVIFVVTK FCLFCFWKFA RKGKKGKRD 529
    Length:529
    Mass (Da):60,906
    Last modified:June 1, 2001 - v1
    Checksum:i8C75277E964690C1
    GO
    Isoform 2 (identifier: Q9BY64-2) [UniParc]FASTAAdd to Basket

    Also known as: II

    The sequence of this isoform differs from the canonical sequence as follows:
         335-335: V → I
         336-529: Missing.

    Show »
    Length:335
    Mass (Da):38,743
    Checksum:iD136BAE9B6A34539
    GO

    Sequence cautioni

    The sequence AAK31809.1 differs from that shown. Reason: Aberrant splicing.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti173 – 1731C → R in BAG70270. (PubMed:19054851)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti365 – 3651L → H.1 Publication
    Corresponds to variant rs4235127 [ dbSNP | Ensembl ].
    VAR_059847
    Natural varianti447 – 4471I → R.1 Publication
    Corresponds to variant rs6843900 [ dbSNP | Ensembl ].
    VAR_060661
    Natural varianti458 – 4581H → D.1 Publication
    Corresponds to variant rs6828191 [ dbSNP | Ensembl ].
    VAR_060662

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei335 – 3351V → I in isoform 2. 1 PublicationVSP_006710
    Alternative sequencei336 – 529194Missing in isoform 2. 1 PublicationVSP_006711Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF177272 mRNA. Translation: AAK31807.1.
    AF177273 mRNA. Translation: AAK31808.1.
    AF177274 mRNA. Translation: AAK31809.1. Sequence problems.
    AB451456 mRNA. Translation: BAG70270.1.
    CCDSiCCDS3528.1. [Q9BY64-1]
    CCDS56330.1. [Q9BY64-2]
    RefSeqiNP_001193933.1. NM_001207004.1. [Q9BY64-2]
    NP_444267.1. NM_053039.1. [Q9BY64-1]
    XP_005265754.1. XM_005265697.1.
    UniGeneiHs.653154.

    Genome annotation databases

    EnsembliENST00000335568; ENSP00000334276; ENSG00000135226. [Q9BY64-1]
    ENST00000511240; ENSP00000427399; ENSG00000135226. [Q9BY64-2]
    GeneIDi54490.
    KEGGihsa:54490.
    UCSCiuc003hej.3. human. [Q9BY64-1]
    uc010ihr.3. human. [Q9BY64-2]

    Polymorphism databases

    DMDMi20140759.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF177272 mRNA. Translation: AAK31807.1 .
    AF177273 mRNA. Translation: AAK31808.1 .
    AF177274 mRNA. Translation: AAK31809.1 . Sequence problems.
    AB451456 mRNA. Translation: BAG70270.1 .
    CCDSi CCDS3528.1. [Q9BY64-1 ]
    CCDS56330.1. [Q9BY64-2 ]
    RefSeqi NP_001193933.1. NM_001207004.1. [Q9BY64-2 ]
    NP_444267.1. NM_053039.1. [Q9BY64-1 ]
    XP_005265754.1. XM_005265697.1.
    UniGenei Hs.653154.

    3D structure databases

    ProteinModelPortali Q9BY64.
    SMRi Q9BY64. Positions 285-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000334276.

    Chemistry

    ChEMBLi CHEMBL6189.
    DrugBanki DB01544. Flunitrazepam.

    Protein family/group databases

    CAZyi GT1. Glycosyltransferase Family 1.

    PTM databases

    PhosphoSitei Q9BY64.

    Polymorphism databases

    DMDMi 20140759.

    Proteomic databases

    MaxQBi Q9BY64.
    PaxDbi Q9BY64.
    PRIDEi Q9BY64.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000335568 ; ENSP00000334276 ; ENSG00000135226 . [Q9BY64-1 ]
    ENST00000511240 ; ENSP00000427399 ; ENSG00000135226 . [Q9BY64-2 ]
    GeneIDi 54490.
    KEGGi hsa:54490.
    UCSCi uc003hej.3. human. [Q9BY64-1 ]
    uc010ihr.3. human. [Q9BY64-2 ]

    Organism-specific databases

    CTDi 54490.
    GeneCardsi GC04P070194.
    H-InvDB HIX0031374.
    HGNCi HGNC:13479. UGT2B28.
    HPAi HPA045108.
    MIMi 606497. gene.
    neXtProti NX_Q9BY64.
    PharmGKBi PA37779.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1819.
    HOGENOMi HOG000220831.
    HOVERGENi HBG004033.
    InParanoidi Q9BY64.
    KOi K00699.
    OMAi KRWSDIQ.
    OrthoDBi EOG7GBFWS.
    PhylomeDBi Q9BY64.
    TreeFami TF315472.

    Enzyme and pathway databases

    Reactomei REACT_6784. Glucuronidation.

    Miscellaneous databases

    GenomeRNAii 54490.
    NextBioi 56811.
    PROi Q9BY64.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9BY64.
    CleanExi HS_UGT2B28.
    Genevestigatori Q9BY64.

    Family and domain databases

    InterProi IPR002213. UDP_glucos_trans.
    [Graphical view ]
    PANTHERi PTHR11926. PTHR11926. 1 hit.
    Pfami PF00201. UDPGT. 1 hit.
    [Graphical view ]
    PROSITEi PS00375. UDPGT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of the UGT2B28 cDNA encoding a novel human steroid conjugating UDP-glucuronosyltransferase."
      Levesque E., Turgeon D., Carrier J.-S., Montminy V., Beaulieu M., Belanger A.
      Biochemistry 40:3869-3881(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    2. "Human protein factory for converting the transcriptome into an in vitro-expressed proteome."
      Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B.
      , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N.
      Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS HIS-365; ARG-447 AND ASP-458.

    Entry informationi

    Entry nameiUDB28_HUMAN
    AccessioniPrimary (citable) accession number: Q9BY64
    Secondary accession number(s): B5BUM0, Q9BY62, Q9BY63
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3