Q9BY64 (UDB28_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glucuronosyltransferase 2B28
Short name=UDPGT 2B28
EC=2.4.1.17

Gene names

Name:

UGT2B28

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme has glucuronidating capacity with steroid substrates such as 5-beta-androstane 3-alpha,17-beta-diol, estradiol, ADT, eugenol and bile acids. Only isoform 1 seems to be active. Ref.1
Catalytic activity	UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside. Ref.1
Subcellular location	Microsome membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein Ref.1.
Tissue specificity	Expressed in the liver, breast and kidney. Ref.1
Sequence similarities	Belongs to the UDP-glycosyltransferase family.
Sequence caution	The sequence AAK31809.1 differs from that shown. Reason: Aberrant splicing.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	xenobiotic metabolic process Inferred from direct assay Ref.1. Source: UniProtKB
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glucuronosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BY64-1)

Also known as: I;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BY64-2)

Also known as: II;

The sequence of this isoform differs from the canonical sequence as follows:
     335-335: V → I
     336-529: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 529	505	UDP-glucuronosyltransferase 2B28		PRO_0000036046
Regions
	Transmembrane	495 – 517	23	Helical; Potential
Amino acid modifications
	Glycosylation	315	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	335	1	V → I in isoform 2.		VSP_006710
	Alternative sequence	336 – 529	194	Missing in isoform 2.		VSP_006711
	Natural variant	365	1	L → H. Ref.2 Corresponds to variant rs4235127 [ dbSNP | Ensembl ].		VAR_059847
	Natural variant	447	1	I → R. Ref.2 Corresponds to variant rs6843900 [ dbSNP | Ensembl ].		VAR_060661
	Natural variant	458	1	H → D. Ref.2 Corresponds to variant rs6828191 [ dbSNP | Ensembl ].		VAR_060662
Experimental info
	Sequence conflict	173	1	C → R in BAG70270. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (I) [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 8C75277E964690C1	FASTA	529	60,906
	10 20 30 40 50 60 MALKWTSVLL LIHLGCYFSS GSCGKVLVWT GEYSHWMNMK TILKELVQRG HEVTVLASSA 70 80 90 100 110 120 SILFDPNDAF TLKLEVYPTS LTKTEFENII MQQVKRWSDI QKDSFWLYFS QEQEILWEFH 130 140 150 160 170 180 DIFRNFCKDV VSNKKVMKKL QESRFDIIFA DAFFPCGELL AALLNIPFVY SLCFTPGYTI 190 200 210 220 230 240 ERHSGGLIFP PSYIPVVMSK LSDQMTFMER VKNMIYVLYF DFWFQMCDMK KWDQFYSEVL 250 260 270 280 290 300 GRPTTLFETM GKADIWLMRN SWSFQFPHPF LPNIDFVGGL HCKPAKPLPK EMEEFVQSSG 310 320 330 340 350 360 ENGVVVFSLG SVISNMTAER ANVIATALAK IPQKVLWRFD GNKPDALGLN TRLYKWIPQN 370 380 390 400 410 420 DLLGLPKTRA FITHGGANGI YEAIYHGIPM VGIPLFWDQP DNIAHMKAKG AAVRLDFHTM 430 440 450 460 470 480 SSTDLLNALK TVINDPSYKE NVMKLSIIQH DQPVKPLHRA VFWIEFVMCH KGAKHLRVAA 490 500 510 520 RDLTWFQYHS LDVIGFLLAC VATVIFVVTK FCLFCFWKFA RKGKKGKRD
	Isoform 2 (II) [UniParc]. Checksum: D136BAE9B6A34539	FASTA	335	38,743
	10 20 30 40 50 60 MALKWTSVLL LIHLGCYFSS GSCGKVLVWT GEYSHWMNMK TILKELVQRG HEVTVLASSA 70 80 90 100 110 120 SILFDPNDAF TLKLEVYPTS LTKTEFENII MQQVKRWSDI QKDSFWLYFS QEQEILWEFH 130 140 150 160 170 180 DIFRNFCKDV VSNKKVMKKL QESRFDIIFA DAFFPCGELL AALLNIPFVY SLCFTPGYTI 190 200 210 220 230 240 ERHSGGLIFP PSYIPVVMSK LSDQMTFMER VKNMIYVLYF DFWFQMCDMK KWDQFYSEVL 250 260 270 280 290 300 GRPTTLFETM GKADIWLMRN SWSFQFPHPF LPNIDFVGGL HCKPAKPLPK EMEEFVQSSG 310 320 330 ENGVVVFSLG SVISNMTAER ANVIATALAK IPQKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the UGT2B28 cDNA encoding a novel human steroid conjugating UDP-glucuronosyltransferase." Levesque E., Turgeon D., Carrier J.-S., Montminy V., Beaulieu M., Belanger A. Biochemistry 40:3869-3881(2001) [PubMed: 11300766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"Human protein factory for converting the transcriptome into an in vitro-expressed proteome." Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B. , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N. Nat. Methods 5:1011-1017(2008) [PubMed: 19054851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS HIS-365; ARG-447 AND ASP-458.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF177272 mRNA. Translation: AAK31807.1. AF177273 mRNA. Translation: AAK31808.1. AF177274 mRNA. Translation: AAK31809.1. Sequence problems. AB451456 mRNA. Translation: BAG70270.1.
IPI	IPI00000498. IPI00219784.
RefSeq	NP_001193933.1. NM_001207004.1. NP_444267.1. NM_053039.1.
UniGene	Hs.653154.
3D structure databases
ProteinModelPortal	Q9BY64.
SMR	Q9BY64. Positions 285-446.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9BY64.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Polymorphism databases
DMDM	20140759.
Proteomic databases
PRIDE	Q9BY64.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000335568; ENSP00000334276; ENSG00000135226.
GeneID	54490.
KEGG	hsa:54490.
UCSC	uc003hej.1. human. uc010ihr.1. human.
Organism-specific databases
CTD	54490.
GeneCards	GC04P070194.
H-InvDB	HIX0031374.
HGNC	HGNC:13479. UGT2B28.
HPA	HPA045108.
MIM	606497. gene.
neXtProt	NX_Q9BY64.
PharmGKB	PA37779.
GenAtlas	Search...
Phylogenomic databases
eggNOG	maNOG15751.
GeneTree	ENSGT00560000076681.
HOVERGEN	HBG004033.
InParanoid	Q9BY64.
OMA	WIHELIY.
OrthoDB	EOG4SJ5DW.
PhylomeDB	Q9BY64.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	Q9BY64.
CleanEx	HS_UGT2B28.
Genevestigator	Q9BY64.
GermOnline	ENSG00000135226. Homo sapiens.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
KO	K00699.
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB01544. Flunitrazepam.
NextBio	56811.
SOURCE	Search...

Entry information

Entry name

UDB28_HUMAN

Accession

Primary (citable) accession number: Q9BY64
Secondary accession number(s): B5BUM0, Q9BY62, Q9BY63

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.