Reviewed, UniProtKB/Swiss-Prot Q9BY64 (UDB28_HUMAN)

Names and origin

Protein names

Recommended name:
    UDP-glucuronosyltransferase 2B28
      Short name=UDPGT 2B28
    EC=2.4.1.17

Gene names

Name:

UGT2B28

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme has glucuronidating capacity with steroid substrates such as 5-beta-androstane 3-alpha,17-beta-diol, estradiol, ADT, eugenol and bile acids. Only isoform 1 seems to be active.
Catalytic activity	UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside.
Subcellular location	Microsome membrane; Single-pass membrane protein Potential. Endoplasmic reticulum membrane; Single-pass membrane protein Potential.
Tissue specificity	Expressed in the liver, breast and kidney.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	xenobiotic metabolic process Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glucuronosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9BY64-1)

Also known as: I;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9BY64-2)

Also known as: II;

The sequence of this isoform differs from the canonical sequence as follows:
     335-335: V → I
     336-529: Missing.

Isoform 3 (identifier: Q9BY64-3)

Also known as: III;

The sequence of this isoform differs from the canonical sequence as follows:
     105-221: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 529	505	UDP-glucuronosyltransferase 2B28		PRO_0000036046
Regions
	Transmembrane	495 – 517	23	Potential
Amino acid modifications
	Glycosylation	315	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	105 – 221	117	Missing in isoform 3.		VSP_006712
	Alternative sequence	335	1	V → I in isoform 2.		VSP_006710
	Alternative sequence	336 – 529	194	Missing in isoform 2.		VSP_006711

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (I) [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 8C75277E964690C1	FASTA	529	60,906
	10 20 30 40 50 60 MALKWTSVLL LIHLGCYFSS GSCGKVLVWT GEYSHWMNMK TILKELVQRG HEVTVLASSA 70 80 90 100 110 120 SILFDPNDAF TLKLEVYPTS LTKTEFENII MQQVKRWSDI QKDSFWLYFS QEQEILWEFH 130 140 150 160 170 180 DIFRNFCKDV VSNKKVMKKL QESRFDIIFA DAFFPCGELL AALLNIPFVY SLCFTPGYTI 190 200 210 220 230 240 ERHSGGLIFP PSYIPVVMSK LSDQMTFMER VKNMIYVLYF DFWFQMCDMK KWDQFYSEVL 250 260 270 280 290 300 GRPTTLFETM GKADIWLMRN SWSFQFPHPF LPNIDFVGGL HCKPAKPLPK EMEEFVQSSG 310 320 330 340 350 360 ENGVVVFSLG SVISNMTAER ANVIATALAK IPQKVLWRFD GNKPDALGLN TRLYKWIPQN 370 380 390 400 410 420 DLLGLPKTRA FITHGGANGI YEAIYHGIPM VGIPLFWDQP DNIAHMKAKG AAVRLDFHTM 430 440 450 460 470 480 SSTDLLNALK TVINDPSYKE NVMKLSIIQH DQPVKPLHRA VFWIEFVMCH KGAKHLRVAA 490 500 510 520 RDLTWFQYHS LDVIGFLLAC VATVIFVVTK FCLFCFWKFA RKGKKGKRD
	Isoform 2 (II). Checksum: D136BAE9B6A34539	FASTA	335	38,743
	10 20 30 40 50 60 MALKWTSVLL LIHLGCYFSS GSCGKVLVWT GEYSHWMNMK TILKELVQRG HEVTVLASSA 70 80 90 100 110 120 SILFDPNDAF TLKLEVYPTS LTKTEFENII MQQVKRWSDI QKDSFWLYFS QEQEILWEFH 130 140 150 160 170 180 DIFRNFCKDV VSNKKVMKKL QESRFDIIFA DAFFPCGELL AALLNIPFVY SLCFTPGYTI 190 200 210 220 230 240 ERHSGGLIFP PSYIPVVMSK LSDQMTFMER VKNMIYVLYF DFWFQMCDMK KWDQFYSEVL 250 260 270 280 290 300 GRPTTLFETM GKADIWLMRN SWSFQFPHPF LPNIDFVGGL HCKPAKPLPK EMEEFVQSSG 310 320 330 ENGVVVFSLG SVISNMTAER ANVIATALAK IPQKI
	Isoform 3 (III). Checksum: 90A554F12D363432	FASTA	412	47,018
	10 20 30 40 50 60 MALKWTSVLL LIHLGCYFSS GSCGKVLVWT GEYSHWMNMK TILKELVQRG HEVTVLASSA 70 80 90 100 110 120 SILFDPNDAF TLKLEVYPTS LTKTEFENII MQQVKRWSDI QKDSFWFQMC DMKKWDQFYS 130 140 150 160 170 180 EVLGRPTTLF ETMGKADIWL MRNSWSFQFP HPFLPNIDFV GGLHCKPAKP LPKEMEEFVQ 190 200 210 220 230 240 SSGENGVVVF SLGSVISNMT AERANVIATA LAKIPQKVLW RFDGNKPDAL GLNTRLYKWI 250 260 270 280 290 300 PQNDLLGLPK TRAFITHGGA NGIYEAIYHG IPMVGIPLFW DQPDNIAHMK AKGAAVRLDF 310 320 330 340 350 360 HTMSSTDLLN ALKTVINDPS YKENVMKLSI IQHDQPVKPL HRAVFWIEFV MCHKGAKHLR 370 380 390 400 410 VAARDLTWFQ YHSLDVIGFL LACVATVIFV VTKFCLFCFW KFARKGKKGK RD

References

[1]

"Isolation and characterization of the UGT2B28 cDNA encoding a novel human steroid conjugating UDP-glucuronosyltransferase." Levesque E., Turgeon D., Carrier J.-S., Montminy V., Beaulieu M., Belanger A. Biochemistry 40:3869-3881(2001) [PubMed: 11300766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING, CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF177272 mRNA. Translation: AAK31807.1. AF177273 mRNA. Translation: AAK31808.1. AF177274 mRNA. Translation: AAK31809.1.
IPI	IPI00000498. IPI00219784. IPI00219785.
RefSeq	NP_444267.1.
UniGene	Hs.653154
3D structure databases
SMR	Q9BY64. Positions 285-446.
ModBase	Search...
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
PTM databases
PhosphoSite	Q9BY64.
Proteomic databases
PRIDE	Q9BY64.
Genome annotation databases
Ensembl	ENSG00000135226. Homo sapiens. [Contig view]
GeneID	54490.
KEGG	hsa:54490.
UCSC	uc003hej.1. human. uc010ihr.1. human.
Organism-specific databases
GeneCards	GC04P070194.
HGNC	HGNC:13479. UGT2B28.
MIM	606497. gene.
PharmGKB	PA37779.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9BY64.
HOVERGEN	Q9BY64.
OMA	Q9BY64. INTATSE.
Enzyme and pathway databases
BRENDA	2.4.1.17. 247.
Reactome	REACT_13433. Biological oxidations.
Gene expression databases
Bgee	Q9BY64.
CleanEx	HS_UGT2B28.
GermOnline	ENSG00000135226. Homo sapiens.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB01544. Flunitrazepam.
NextBio	56811.
SOURCE	Search...

Entry information

Entry name

UDB28_HUMAN

Accession

Primary (citable) accession number: Q9BY64
Secondary accession number(s): Q9BY62, Q9BY63

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	June 1, 2001
Last modified:	July 7, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)