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Protein

Eukaryotic translation initiation factor 2A

Gene

EIF2A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins.1 Publication

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • ribosome binding Source: UniProtKB
  • translation initiation factor activity Source: UniProtKB
  • tRNA binding Source: UniProtKB

GO - Biological processi

  • positive regulation of signal transduction Source: Ensembl
  • protein phosphorylation Source: Ensembl
  • regulation of translation Source: UniProtKB
  • response to amino acid starvation Source: Ensembl
  • ribosome assembly Source: UniProtKB
  • SREBP signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Enzyme and pathway databases

SIGNORiQ9BY44.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2A
Short name:
eIF-2A
Alternative name(s):
65 kDa eukaryotic translation initiation factor 2A
Cleaved into the following chain:
Gene namesi
Name:EIF2A
ORF Names:CDA02, MSTP004, MSTP089
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:3254. EIF2A.

Subcellular locationi

GO - Cellular componenti

  • blood microparticle Source: UniProtKB
  • cell-cell adherens junction Source: BHF-UCL
  • cytoplasm Source: UniProtKB
  • eukaryotic translation initiation factor 2 complex Source: UniProtKB
  • extracellular space Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi83939.
OpenTargetsiENSG00000144895.
PharmGKBiPA143485453.

Polymorphism and mutation databases

BioMutaiEIF2A.
DMDMi209572747.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004244661 – 585Eukaryotic translation initiation factor 2AAdd BLAST585
Initiator methionineiRemoved; alternate1 Publication
ChainiPRO_00002860762 – 585Eukaryotic translation initiation factor 2A, N-terminally processedAdd BLAST584

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei2N-acetylalanine; in Eukaryotic translation initiation factor 2A, N-terminally processed1 Publication1
Modified residuei5PhosphothreonineCombined sources1
Modified residuei503PhosphoserineCombined sources1
Modified residuei506PhosphoserineCombined sources1
Modified residuei517PhosphoserineCombined sources1
Modified residuei518PhosphothreonineCombined sources1
Modified residuei526PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9BY44.
MaxQBiQ9BY44.
PaxDbiQ9BY44.
PeptideAtlasiQ9BY44.
PRIDEiQ9BY44.

PTM databases

iPTMnetiQ9BY44.
PhosphoSitePlusiQ9BY44.
SwissPalmiQ9BY44.

Expressioni

Tissue specificityi

Widely expressed. Expressed at higher level in pancreas, heart, brain and placenta.1 Publication

Gene expression databases

BgeeiENSG00000144895.
CleanExiHS_EIF2A.
ExpressionAtlasiQ9BY44. baseline and differential.
GenevisibleiQ9BY44. HS.

Organism-specific databases

HPAiHPA036256.

Interactioni

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL

Protein-protein interaction databases

BioGridi123822. 49 interactors.
DIPiDIP-40272N.
IntActiQ9BY44. 8 interactors.
MINTiMINT-3063792.
STRINGi9606.ENSP00000417229.

Structurei

3D structure databases

ProteinModelPortaliQ9BY44.
SMRiQ9BY44.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati56 – 100WD 1Add BLAST45
Repeati101 – 159WD 2Add BLAST59
Repeati160 – 210WD 3Add BLAST51
Repeati211 – 264WD 4Add BLAST54
Repeati265 – 306WD 5Add BLAST42
Repeati307 – 348WD 6Add BLAST42
Repeati349 – 391WD 7Add BLAST43

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili531 – 582Sequence analysisAdd BLAST52

Sequence similaritiesi

Belongs to the WD repeat EIF2A family.Curated
Contains 7 WD repeats.Curated

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2315. Eukaryota.
COG5354. LUCA.
GeneTreeiENSGT00730000111053.
HOGENOMiHOG000193775.
HOVERGENiHBG107842.
InParanoidiQ9BY44.
KOiK15026.
OMAiCARVVTN.
OrthoDBiEOG091G04PE.
PhylomeDBiQ9BY44.
TreeFamiTF105866.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR011387. TIF2A.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08662. eIF2A. 1 hit.
[Graphical view]
PIRSFiPIRSF017222. eIF2A. 1 hit.
SUPFAMiSSF50978. SSF50978. 2 hits.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9BY44-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPSTPLLTV RGSEGLYMVN GPPHFTESTV FPRESGKNCK VCIFSKDGTL
60 70 80 90 100
FAWGNGEKVN IISVTNKGLL HSFDLLKAVC LEFSPKNTVL ATWQPYTTSK
110 120 130 140 150
DGTAGIPNLQ LYDVKTGTCL KSFIQKKMQN WCPSWSEDET LCARNVNNEV
160 170 180 190 200
HFFENNNFNT IANKLHLQKI NDFVLSPGPQ PYKVAVYVPG SKGAPSFVRL
210 220 230 240 250
YQYPNFAGPH AALANKSFFK ADKVTMLWNK KATAVLVIAS TDVDKTGASY
260 270 280 290 300
YGEQTLHYIA TNGESAVVQL PKNGPIYDVV WNSSSTEFCA VYGFMPAKAT
310 320 330 340 350
IFNLKCDPVF DFGTGPRNAA YYSPHGHILV LAGFGNLRGQ MEVWDVKNYK
360 370 380 390 400
LISKPVASDS TYFAWCPDGE HILTATCAPR LRVNNGYKIW HYTGSILHKY
410 420 430 440 450
DVPSNAELWQ VSWQPFLDGI FPAKTITYQA VPSEVPNEEP KVATAYRPPA
460 470 480 490 500
LRNKPITNSK LHEEEPPQNM KPQSGNDKPL SKTALKNQRK HEAKKAAKQE
510 520 530 540 550
ARSDKSPDLA PTPAPQSTPR NTVSQSISGD PEIDKKIKNL KKKLKAIEQL
560 570 580
KEQAATGKQL EKNQLEKIQK ETALLQELED LELGI
Length:585
Mass (Da):64,990
Last modified:October 14, 2008 - v3
Checksum:i63D63C0676F232C9
GO
Isoform 2 (identifier: Q9BY44-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-213: Missing.
     214-219: ANKSFF → MFQEV

Note: No experimental confirmation available.
Show »
Length:371
Mass (Da):41,339
Checksum:i0D4454F18732DBD2
GO
Isoform 3 (identifier: Q9BY44-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     34-58: Missing.

Note: No experimental confirmation available.
Show »
Length:560
Mass (Da):62,289
Checksum:iAE5E408990B0CC21
GO
Isoform 4 (identifier: Q9BY44-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     98-158: Missing.

Note: No experimental confirmation available.
Show »
Length:524
Mass (Da):57,994
Checksum:iE6F35813714656EF
GO

Sequence cautioni

The sequence AAK14926 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAM83402 differs from that shown. Reason: Frameshift at positions 82 and 90.Curated
The sequence AAQ13506 differs from that shown. Reason: Frameshift at position 513.Curated
The sequence AAQ13612 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7L → F in AAQ13612 (Ref. 4) Curated1
Sequence conflicti14 – 16EGL → LRT in AAQ13612 (Ref. 4) Curated3
Sequence conflicti54G → A in AAM83402 (PubMed:12133843).Curated1
Sequence conflicti55N → I in AAM83402 (PubMed:12133843).Curated1
Sequence conflicti84S → P in BAB55058 (PubMed:14702039).Curated1
Sequence conflicti150V → A in AAQ13612 (Ref. 4) Curated1
Sequence conflicti173F → S in AAQ13612 (Ref. 4) Curated1
Sequence conflicti261T → P in AAM83402 (PubMed:12133843).Curated1
Sequence conflicti263G → F in AAM83402 (PubMed:12133843).Curated1
Sequence conflicti268V → L in AAM83402 (PubMed:12133843).Curated1
Sequence conflicti292Y → S in AAM83402 (PubMed:12133843).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03206697T → S.5 PublicationsCorresponds to variant rs1132979dbSNPEnsembl.1
Natural variantiVAR_032067582E → K.1 PublicationCorresponds to variant rs17850813dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0249751 – 213Missing in isoform 2. 1 PublicationAdd BLAST213
Alternative sequenceiVSP_05604734 – 58Missing in isoform 3. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_05604898 – 158Missing in isoform 4. 1 PublicationAdd BLAST61
Alternative sequenceiVSP_024976214 – 219ANKSFF → MFQEV in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF497978 mRNA. Translation: AAM83402.1. Frameshift.
AK027356 mRNA. Translation: BAB55058.1.
AK298586 mRNA. Translation: BAG60776.1.
AK293993 mRNA. Translation: BAG57357.1.
AF212241 mRNA. Translation: AAK14926.1. Different initiation.
AF109358 mRNA. Translation: AAQ13506.1. Frameshift.
AF172818 mRNA. Translation: AAQ13612.1. Different initiation.
AC107426 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78830.1.
CH471052 Genomic DNA. Translation: EAW78831.1.
BC011885 mRNA. Translation: AAH11885.1.
CCDSiCCDS46935.1. [Q9BY44-1]
CCDS82859.1. [Q9BY44-4]
CCDS82861.1. [Q9BY44-3]
RefSeqiNP_001305972.1. NM_001319043.1. [Q9BY44-3]
NP_001305973.1. NM_001319044.1. [Q9BY44-4]
NP_001305974.1. NM_001319045.1.
NP_001305975.1. NM_001319046.1. [Q9BY44-2]
NP_114414.2. NM_032025.4. [Q9BY44-1]
UniGeneiHs.655782.

Genome annotation databases

EnsembliENST00000406576; ENSP00000385292; ENSG00000144895. [Q9BY44-4]
ENST00000460851; ENSP00000417229; ENSG00000144895. [Q9BY44-1]
ENST00000487799; ENSP00000420537; ENSG00000144895. [Q9BY44-3]
GeneIDi83939.
KEGGihsa:83939.
UCSCiuc003eya.4. human. [Q9BY44-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF497978 mRNA. Translation: AAM83402.1. Frameshift.
AK027356 mRNA. Translation: BAB55058.1.
AK298586 mRNA. Translation: BAG60776.1.
AK293993 mRNA. Translation: BAG57357.1.
AF212241 mRNA. Translation: AAK14926.1. Different initiation.
AF109358 mRNA. Translation: AAQ13506.1. Frameshift.
AF172818 mRNA. Translation: AAQ13612.1. Different initiation.
AC107426 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78830.1.
CH471052 Genomic DNA. Translation: EAW78831.1.
BC011885 mRNA. Translation: AAH11885.1.
CCDSiCCDS46935.1. [Q9BY44-1]
CCDS82859.1. [Q9BY44-4]
CCDS82861.1. [Q9BY44-3]
RefSeqiNP_001305972.1. NM_001319043.1. [Q9BY44-3]
NP_001305973.1. NM_001319044.1. [Q9BY44-4]
NP_001305974.1. NM_001319045.1.
NP_001305975.1. NM_001319046.1. [Q9BY44-2]
NP_114414.2. NM_032025.4. [Q9BY44-1]
UniGeneiHs.655782.

3D structure databases

ProteinModelPortaliQ9BY44.
SMRiQ9BY44.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123822. 49 interactors.
DIPiDIP-40272N.
IntActiQ9BY44. 8 interactors.
MINTiMINT-3063792.
STRINGi9606.ENSP00000417229.

PTM databases

iPTMnetiQ9BY44.
PhosphoSitePlusiQ9BY44.
SwissPalmiQ9BY44.

Polymorphism and mutation databases

BioMutaiEIF2A.
DMDMi209572747.

Proteomic databases

EPDiQ9BY44.
MaxQBiQ9BY44.
PaxDbiQ9BY44.
PeptideAtlasiQ9BY44.
PRIDEiQ9BY44.

Protocols and materials databases

DNASUi83939.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000406576; ENSP00000385292; ENSG00000144895. [Q9BY44-4]
ENST00000460851; ENSP00000417229; ENSG00000144895. [Q9BY44-1]
ENST00000487799; ENSP00000420537; ENSG00000144895. [Q9BY44-3]
GeneIDi83939.
KEGGihsa:83939.
UCSCiuc003eya.4. human. [Q9BY44-1]

Organism-specific databases

CTDi83939.
DisGeNETi83939.
GeneCardsiEIF2A.
H-InvDBHIX0200477.
HGNCiHGNC:3254. EIF2A.
HPAiHPA036256.
MIMi609234. gene.
neXtProtiNX_Q9BY44.
OpenTargetsiENSG00000144895.
PharmGKBiPA143485453.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2315. Eukaryota.
COG5354. LUCA.
GeneTreeiENSGT00730000111053.
HOGENOMiHOG000193775.
HOVERGENiHBG107842.
InParanoidiQ9BY44.
KOiK15026.
OMAiCARVVTN.
OrthoDBiEOG091G04PE.
PhylomeDBiQ9BY44.
TreeFamiTF105866.

Enzyme and pathway databases

SIGNORiQ9BY44.

Miscellaneous databases

GeneWikiiEIF2A.
GenomeRNAii83939.
PROiQ9BY44.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000144895.
CleanExiHS_EIF2A.
ExpressionAtlasiQ9BY44. baseline and differential.
GenevisibleiQ9BY44. HS.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR011387. TIF2A.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08662. eIF2A. 1 hit.
[Graphical view]
PIRSFiPIRSF017222. eIF2A. 1 hit.
SUPFAMiSSF50978. SSF50978. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiEIF2A_HUMAN
AccessioniPrimary (citable) accession number: Q9BY44
Secondary accession number(s): A8MPS6
, B4DF96, B4DQ14, D3DNI9, Q5QTR2, Q7Z4E9, Q8NFM1, Q96EW9, Q96K81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: October 14, 2008
Last modified: November 2, 2016
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was initially thought to constitute the ortholog of prokaryotic IF-2 (infB) protein.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.